Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.

참복과 어류 1미기록종, Takifugu oblongus (폭포무늬복: 국명신칭)의 형태 및 골격

한경호,김춘철,윤성민 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

이 실험에 사용된 Takifugu oblongus (Bloch)는 2001년 7월에 동중국해에서 중저층 트롤로 채집된 5마리(체장 195.0∼265.3 ㎜)로 표본의 형질을 조사하기 위하여 외부형태를 관찰한 후에 계수·계측하였으며, 골격의 형태는 삶아서 채골하여 관찰하였다. T. oblongus는 등지느러미 줄기수는 12∼13개이며, 뒷지느러미 줄기수는 10∼11개, 가슴지느러미 줄기수는 16개, 꼬리지느러미 줄기수는 11개이다. 척추골은 19∼20개(미부봉상골 포함), 새조골은 6개이다. 그리고 체색은 등쪽에서 배쪽으로 폭포무늬의 황색의 띠가 가로줄로 내려와 있었고, 등지느러미 바로 앞쪽의 경우 띠가 한선으로 연결된 점이 특징이다. 이러한 특징으로 한국 미기록 어종인 T. oblongus을 "폭포무늬복"로 신칭하였다. The oblong toadfish, Tukifugu oblongus (Bloch), of the family Tetraodontidae was studied for the first time in Korea. The specimens were collected by an intermediate-bottom-layer trawl in East China Sea in July 2001. The species is characterized by brownish above with pale spots (waterfall design), yellowish white below, narrow dark bars on sides, teeth fused into a beaklike dental plate with a median suture giving two teeth in each jaw, 12 to 13 dorsal fin rays, 16 pectoral fin rays, 10 to 11 anal fin rays, 11 caudal fin rays, 19 to 20 vertebrae and 6 branchiostegal rays. A new Korean name "Pok-po-moo-nyi-bok" is proposed for the species.

상어 간유 흡인에 의한 급성 지방성 폐렴 1예 : 증례보고

임성경,옥철호,김희규,장태원,정만홍 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

저자들은 상어 간유를 과량 복용하고 기침, 호흡곤란을 주소로 내원한 48세 여자 환자에서 방사선 소견과 기관지 폐포 세척액 검사를 통해 급성 지방성 폐렴으로 진단하고 스테로이드에 불응하는 경과를 취하자 우측 전폐 기관지 폐포 세척술을 시행하여 폐 병변과 증상의 호전을 보인 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Acute lipoid pneumonia is uncommon condition of the lungs that results from the aspiration of vegetable, animal, or mineral oils. Shark liver oil (so called Squalene) is taken as a traditional remedy in some Asian countries, and is used widely also in cosmetics. It results in foreign body type inflammatory reaction of the lung and can show various clinical presentations from asymptomatic incidental finding to severe pneumonia leading to acute respiratory failure. Although many cases have been reported, severe lipoid pneumonia requiring steroid or whole lung lavage for treatment is rare. We experienced a case of acute lipoid pneumonia of 48 year old female with dyspnea and cough caused by regular ingestion of large amount Shark liver oil, treated by whole lung lavage. We reported this case with a brief review of related literatures.

하순에 발생한 우췌성상피세포암의 Karapandzic flap을 이용한 치험례

서경성,류동목,이상철,김여갑,이백수 大韓顎顔面成形再建外科學會 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.6

The verrucous carcinoma which was classified by Ackerman in 1948 is generally well differentiated and slow growing. After its radical surgery, it is rarely recurrent of carcinoma. Tobacco chewing is a most important factor in the etiology of these lesion. It frequently affect males over 55 year old age and usually appears on buccal and mandibular alveolar mucosa. The continuing effort by plastic surgeon to reconstruc lips successfully has been accompanied by numerous publications on the subject. More recent communications have dealt with modifications or refinements of existing procedures. In 1974, Karapandzic reported the use of arterialied flaps to reconstruct lips following excisions of cancers. We have treated 65 year old male patient rapid growing verrucous carcinoma on lower lip with Karapandzic flap. Postoperatively the results showed good prognosis. So we report the result of its treatment and case with review of literature.

급성췌장염을 동반한 특발성 양측성 횡격막 마비 1례

박주경,소정일,류진호,김성근,허탁,민용일 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Bilateral diaphragmatic paralysis(BDP) is a rare disorder, which can be secondary to spinal cord injury, motor neuron disease, myopathy, noninfectious polyneuropathy, infection, iced saline cardioplegia performed during cardiac surgery, or idiopathic causes. There may be typical presentations such as dyspnea, paradoxical respiratory movement, and hypercapnic respiratory failure. It needs to exclude above secondary causes to consider idiopathic bilateral diaphragmatic paralysis. We report a 51-years-old man who presented with idiopathic bilateral diaphragmatic paralysis combined with acute pancreatitis. We couldn't find out the association of these two clinical conditions. The patient was improved by mechanical ventilation.

청소년의 자전거 운동이 운동성 심장 허혈 증세에 미치는 영향

고성경 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.1

[Purpose] The purpose of this study was to investigate the ischemic cardiac symptom during the treadmill exercise in adolescent cycle athletes. [Methods] Subjects were 13-16 years old and healthy. They were classified into three groups: sedentary control (n=14), leisure-exercise (n=15), cycle-athletes (n=14). During the maximal treadmill exercise, ST segment depression was measured in V5 lead of ECG. Duke score and annual cardiovascular mortality(CVM) were calculated by Mark' Equations. One-way ANOVA was used to analyze the differences among the groups. [Results.] HR, SBP, DBP and blood lipid profiles were not difference among the groups, but maximal oxygen uptake and exercise duration of cycle-athletes were significantly higher than control. ST segment of control during treadmill exercise were significantly more depressed than leisure-exercise(at exercise 3min, maximal exercise and recovery 3min) and cycle-athletes(at rest, exercise 3,6min, maximal exercise and recovery 3min). ST segment depression at maximal exercise was correlated with total cholesterol(r=-.534) and triglycerides(r=-.589) in control, and total cholesterol(r=-.536) in leisure-exercise. CVM during 5 years were 2.94% in control which was significantly higher than -1.20% of cycle-athletes. [Conclusion] In conclusion, participation in physical training or leisure sports during adolescence seem to prevent ischemic cardiac symptom with exercise.

流體로 채워진 圓筒 Channel內 捧 集合體의 振動解析

鄭炅勳,李聖哲 全北大學校 1993 論文集 Vol.36 No.-

Free vibration of the tube bundle in a channel filled with stationary liquid is investigated. Added mass coefficients which are based on velocity potential theory are calculated and used to computing the natural frequencies and mode shapes of tube bundle in liquid. The natural frequencies of the tube bundle are obtained as a function of the spacing between tubes and verified by an experiment. The experimental results show good agreement with the computer predictions. As the spacing between the tubes is small, namely gap to diameter(G/D) becomes small, coupled natural frequencies are widely distributed. A wide spacing tube bundle goes the results of single tube in an infinite liquid.

수학과 교육과정 실제 및 분석

김민경,노선숙,유현주,조성민,차인숙 이화여자대학교 사범대학 교과교육연구소 2001 교과교육학연구 Vol.5 No.1

21세기 지식기반사회는 자기 주도적으로 정보를 처리하고 해석하며 판단하는 능력과 지적 가치를 창조할 수 있는 능력을 길러주는 학교수학 교육을 요구하고 있다. 이에 수학교육과정에 담겨져야 할 수학적 지식의 구조․내용․범위, 지식기반 사회에 적합한 수학과 교수-학습, 평가, 교사역할 등 역시 새로운 관점에서 재조명될 필요가 있으며, 이러한 연구는 학교 현장에 대한 이해에서 출발하고 개인의 필요성과 사회의 요구를 반영한 것이어야 한다. 이에 본 연구에서는 지식기반사회에 대비하기 위한 수학과 교육과정 개발 연구의 기초 단계로 교사, 학생, 학부모를 대상으로 현장 설문 조사를 전국 규모로 실시하였다. 설문 조사는 Goodlad의 학교 교육과정 탐구의 개념적 구도를 바탕으로 한국 교육의 실정을 반영하여 설계되었다. 수학 교육과정 실체의 다양한 국면을 조사하기 위한 설문 내용은 목적 및 목표, 교과내용, 교수-학습방법, 평가, 수학적 지식의 성격, 교육정보화, 교사교육, 사교육, 교육과정일반으로 구성되었다. 본 고에서는 Goodlad의 교육과정 탐구모형과 그 모형에 기초하여 작성된 본 연구의 수학교육과정 탐구모형, 실시된 설문조사의 방법 및 절차를 소개하였고, 설문조사의 주된 결과를 토대로 수학교육과정 개발연구에의 시사점을 찾고자 하였다. This study is to provide suggestions for the development of better mathematics curriculum model of Korea based on a national survey of teachers (527), students (1314), and parents (638). It is our belief that understanding the perceptions and experiences of those involved in the process of teaching and learning of mathematics education needs to be the first priority in any effort to explore better mathematics curriculum model. For the study, Goodlad's conceptual system for curriculum inquiry has been used, but modified to take into account Korean context and the nature of mathematics curriculum. In this paper we introduce the Goodlad's curriculum inquiry model and mathematics curriculum inquiry model based on the Goodlad's conceptual system as well as the survey process. We made few recommendations from the main results of the survey for the research and development of mathematics curriculum model in Korea.

복섬(Takifugu niphobles)자치어의 골격발달

한경호,오성현,서원일 한국어류학회 2003 韓國魚類學會誌 Vol.15 No.3

복섬의 부화 직 후 자어는 평균 전장 2.14 ㎜로 골격의 골화가 전혀 일어나지 않았으며, 부화 후 1일째 평균 전장 2.38 ㎜의 자어는 두개골에 복추골, 섭이와 관련하는 턱부분에 전상악골과 치골이 먼저 골화가 시작되었다. 부화 후 10일째 자어는 평균 전장 4.24㎜로, 두개골에 전이골과 익이골이 골화되었고, 내장골의 새개부에는 하새개골, 간새개골이 골화하여 새개부가 완성되고, 견대부의 후쇄골이 골화하기 시작하였다. 부화 후 28일째 후기자어의 평균 전장은 7.60㎜로 두개골에 상이골과 상후두골이 골화하여 두개골이 완성되었고, 척추골, 미골 및 담기골이 거의 동시에 골화되었다. 부화 후 35일째 치어는 평균 전장이 8.60㎜로 구개부에 후익상골이 골화하여 내장골이 골화가 완료되므로서, 모든 골격의 골화가 완성되었다. Eggs of Takifugu niphobles (Jordan et Snyder) collected on the shores of Dolsan, Yeosu, Korea, in July 1998, were artificially hatched and the larvae reared in the laboratory to investigate osteological development of larvae and juveniles. The newly hatched larvae attained a mean size of 2.38 ㎜ in total length (TL) and had an ossified dentary, premaxillary and clavicle at this stage. At 10 days after hatching, the larvae (mean 4.24 ㎜ in TL) had ossified prootic, pterotic, subopercle, and interopercle, and the postclavicle had begun to ossify. The ossification of the cranium, vertebrae, caudal skeleton and pterygiophore was complete in the larvae (mean 7.60 ㎜ in TL) at 28 days, and ossification of all bones was complete in the juveniles (mean 8.61 ㎜ in TL) at 35 days.

경추손상이 의심되는 환자에서 경추전방 연부조직 폭 측정의 의의

박주경,김성근,윤한덕,허탁,조석주,민용일 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

Prevertebral soft tissue swelling of cervical spine lateral radiogram is well known as an indirect evidence of occult cervical spine injury. But the clinical and statistical value of it has been of debate. We tried to analyse the value of cervical prevertebral soft tissue width as a screening test for cervical spine injury by comparison of two categorized study groups; the control group, traumatized patients without cervical spine injury and the injury and the injury group, traumatized patients with bony cervical injury. The injury group consisted of patients admitted between September 1993 and December 1994 with an ED diagnosis of bony cervical injury. The control group consisted of patients admitted between January 1994 and June 1994 who received cervical spine lateral radiograph because of suspicion of cervical spine injury or as a routine check. In both group, we sampled the patients who received cervical spine lateral rediograph within 24 hours after injury and excluded the patients less than 15 years old and more than 65 years old, In the control group, we confirmed the patients had no problem in cervical spine of follow-up. 101 control patients and 68 injury patients were identified. The injury group was further divided into two subgroups: those with injuries at C1-C3 to the upper injury subgroup and those with injuries at C4-C7 to the lower injury sub group. The prevertebral soft tissue widths of injury group were larger than of control group at C2(mean 6.1mm versus 4.5mm) and also at C6(mean 13.4mm versus 11.2mm). Especially upper injury subgroup had quite large value of C2 prevertebral soft tissue width(mean 14.1mm). Nevertheless, both in C2 and C6, there is no cutoff value with acceptable sensitivity and specificity as a screening test. But the 7mm at C2 and 14mm at C6 had high specificity(90%) and were thought to be a good radiologic indicators of further evaluation.