응급실에서 기관내 삽관후 발생한 후두육아종 1례

조남수,김용배,조수형,송민엽,송성용 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

Endotracheal intubation has been used widely for the maintenance of airway in the emergency patient and CPR in the acute airway obstruction patient and to treat respiratory insufficiency. Endotracheal intubation has many advantages but it may cause several complications which rate about 4% of endotracheal intubation patient. Among these complications, laryngeal granuloma is a relatively rare complication arising about 1 case per 10,000∼20,000 cases. Authors report a case of laryngeal granuloma which developed after endotracheal intubation required during emergency treatment. Case: A 55-year old female patient visited Chosun University Hospital Emergency Room complaining of dyspnea, cyanosis and sweating on Feb. 8th 1995. According to past history, she had a little obesity, dyspnea on exercise and mild hypertension. At arrival, blood pressure was 240/110mmHg, pulse rate 150 rates/min, respiratory rate 12/min, body temperature 36.5℃ and cyanosis with sweating on whole body was observed. And the mental state was semicoma. At 5 minutes after arrival, sudden respiratory arrest developed and immediate endotracheal intubation which emergency treatment was performed it was difficult to intubate endotracheally due to short neck and obesity. At 1 hour after arrival, general condition of the patient recovered well and extubation was done. The patient was transferred to internal medicine department and discharged after 12 days of medical treatment. At 2 months after discharge, the patient felt a progressive hoarseness and foreign body sensation of larynx. She was diagnosed as laryngeal granuloma at ENT department of Chosun University. She underwent the surgical operation and discharged in good health after 7 days of admission.

Changes in cAMP Response and Testosterone to 17β-Estradiol to FSH in Isolated Rat Sertoli Cells-Age and Dose Related Differences

Cho, Sung Hoon,Lee, Byung Churl,Seong, Sang Min CATHOLIC MEDICAL CENTER 1986 Bulletin of the Clinical Research Institute Vol.14 No.1

The Seroli cell was identified as> the main primary target site for the action of follicle stimulating hormone (FSH) in the testis. FSH has been known to bind specifically to Sertoli cell and to evoke a series of biochemical responses such as increased accumulation of cyclic AMP, production of androgen binding protein, and steroid metabolism. Recently age related changes in FSH stimulation of cAMP and aromatization of testosterone of 17β-estradiol were demonstrated. The purpose of this study was to investigate the changes in cAMP and testosterone to 17β-estradiol aromatization responses of FSH with dose differences in isolated rat Sertoli cells of different age. Methods for preparation of rat Sertoli cells were adapted from those used to prepare rat Sertoli cells. Homogenous preparations of primary Sertoli cell cultures were obtained from the testis of the rat of different age: prepubertal (14 days old rat), pubertal (18 days old rat) and adult (60 days old rat). For cAMP determinations, cells were stimulated for 30 min at 37?C with different dose of FSH in media containing 0.1 mg/m]l l-methyl-3-isobutylxanthine. cAMP assays were performed in duplicate using radioimmunoassay kit. FSH stimulated aromatization of testosterone of 17j3-estradiol was determined in cells stimulated with different dose of FSH in media containing 0.5 μmol/ml testosterone. 170-estradiol assays were performed in duplicate by means of specific radioimmunoassay. The results were as follows: 1. Sertoli cells from adult rats responded to FSH stimulation with over 30-fold rise at 5 min. At 30 min, cAMP is significantly lower and down to baseline level by 60 min. 2. There were age-related differences in Sertoli cell cAMP response after 30 min of FSH stimulation. Sertoli cells from pubertal rats responded most sensitively to FSH with a dose response relationship. 3. The heighest aromatase enzyme activity in converting testosterone to 17(3-estradiol was found in pubertal rat Sertoli cells. Sertoli cells from pubertal rats demonstrated a dose-response of FSH stimulation of testosterone to estradiol conversion. In conclusion, these results demonstrate that the age and dose-related differences in rat Sertoli cell response to FSH, and also provide direct evidence that the Sertoli cells represent a primary target site for FSH activity in the testes.

Purification and Characterization of Helicobacter pylori ${\gamma}$-Glutamyltranspeptidase

Song, Jae-Young,Choi, Yeo-Jeong,Kim, Jeong-Min,Kim, Yoo-Ree,Jo, Jin-Seong,Park, Jin-Sik,Park, Hee-Jin,Song, Yun-Gyu,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Youn, Hee-Shang,Cho, Myung-Je,Rhee, Kw The Korean Society for Microbiology 2011 Journal of Bacteriology and Virology Vol.41 No.4

Gamma-glutamyltranspeptidase (GGT) was purified to electrophoretic homogeneity from the cell extract of H. pylori. The purified enzyme consisted of heavy and light subunits with molecular weights of 38 kDa and 21 kDa, respectively. N-terminal amino acid sequence of heavy and light subunits revealed that H. pylori GGT was processed into 3 parts for a signal peptide of 27 amino acid residues, a heavy subunit of 352 residues, and a light subunit of 188 residues during translation. The reaction rate for hydrolysis of ${\gamma}$-GpNA was 84.4 ${\mu}mol/min$ per milligram of protein, and that for the ${\gamma}$-glutamyl transfer from ${\gamma}$-GpNA to gly-gly was 23.8 ${\mu}mol/min$ per milligram of protein. The apparent Km values of H. pylori GGT for ${\gamma}$-glutamyl compounds were on the order of $10^{-3}$ to $10^{-4}$ M and those for acceptor peptides and amino acids were on the order of $10^{-1}$ to $10^{-2}$ M. The GGT protein kept approximately 80% of the initial enzymatic activity on incubation at $60^{\circ}C$ for 15 min. The optimum temperature and pH for reactions of both hydrolysis and transpeptidation were $40^{\circ}C$ and 9.0, respectively. The transpeptidation and hydrolysis reactions catalyzed by H. pylori GGT were strongly inhibited by L-Gln and moderately inhibited by L-Ala, L-Ser, ${\beta}$-chloro-L-Ala, and L-Glu. These results demonstrated that the biochemical properties of H. pylori GGT are different from those of other bacterial GGTs. Further, H. pylori GGT might degrade glutathione in the gastric mucous layer of humans if the enzyme could be secreted in the bacterial niches.

일 장기 수용시설 노인들의 인지기능장애와 우울증상의 유병율

조맹제,함봉진,이부영,김성윤,신영민,이창인 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.5

목 적 : 본 연구는 수용시설의 노인들에서 인지기능장애와 우울증상의 유병율을 조사하기 위한 것이다. 방 법 : 한국의 한 수용시설인 꽃동네에 거주하는 60세 이상 노인 169명을 대상으로 한국판 Mini-Mental State Examination(MMSE-K). Short Portable Mental State Questionnaire(SPMSQ), 축약판 Geriatric Depression Scale(SGDS). 그리고 the Center for Epidemiologic Studies Depression (CES-D)을 적용하였다. 결 과 : 전체 169명중 153명(남자=72명, 여자=79명, 그리고 누락=2명)이 검사를 완성하였다. 연령의 범위는 60에서 102세였고, 평균연령은 74.17±8.27(남자71.76±8.14. 여자76.14±7.78)세였다. 교육기간의 범위는 무학에서 16년이었고, 평균교육기간은 3.04±3.93(남자 4.13±4.23, 여자 2.01±3.38)년이었다. 인지기능장애의 유병율은 MMSE-K≤23의 기준으로 56.2%, SPMSQ≤6의 기준으로 26.1% 로 평가되었다. 비교적 분명한 인지기능장애의 기준으로 MMSE-K≤19를 적용하였을 경우 유병율은 25.5%로 이는 SPMSQ≤6을 기준으로 하여 얻은 결과와 유사한 수준이었다. 우울증상의 유병율은 SGDS≥10의 기준으로 19%, CES-D≥25의 기준으로 23.5%로 평가되었다. 비교적 가벼운 우울증상까지 선별하기 위하여 SGDS≥8을 기준으로 할 경우 유병율은 39.2%로 이는 CES-D≥20을 기준으로 하여 얻은 결과와 일치하는 수준이었다. MMSE-K와 SPMSQ, SGDS와 CES-D, MMES-K와CES-D, MMES-K와 SGDS, 그리 고·SPMSQ와 SGDS 사이에 의미있는 상관관계가 관찰되었다. 결 론 : 수용시설 노인들에서 인지기능장애와 우울증상의 유병율은 매우 높은 수준이며, 이러한 결과는 수용시설 노인들에 대한 보다 집중적의 의료서비스가 필요함을 시사한다. Objectives : This study was to investigate the prevalence of cognitive impairment and depressive symptomatology among the long-term institutionalized elderly people. Methods : Mini-Mental State Examination-Korean version(MMSE-K), Short Portable Mental State Questionnaire(SPMSQ), Short version of Geriatric Depression Scale(SGDS), and the Center for Epidemiologic Studies Depression(CES-D) were administerd to 169 elderly residents in an institution, 'Got Dong Rae'. Results : One hundred fifty-three(males=72, females=79, and missing=2) completed the entire examination. Age ranged from 60 to 102 years and the mean of age was 74.17±8.27(males 71.76±8.14, females 76.14±7.78) years. Duration of education ranged from 0 to 16 years and the mean of duration of education was 3.04±3.93(males 4.13±4.23, females 2.01±3.38) years. The prevalence of cognitive impairment was estimated as 56.2% by MMSE-K≤23 and 26.1% by SPMSQ≤6. The prevalence of relatively definite cognitive impairment defined by MMSE-K≤19 was 25.5%, which was similar to the result achieved by using SPMSQ≤6. The prevalence of depressive symptomatology was estimated as 19% by SGDS≥10 and 23.5% by CES-D≥25. In the case of using SGDS≥8 as cutoff-point to screen mild as well as severe depressive symptomatology, the prevalence was 39.2%. It was identical to the result achieved by using CES-D≥20. Significant correlations were observed between MMSE-K and SPMSQ, between SGDS and CES-D, between MMSE-K and CES-D, between MMSE-K and SGDS, and between SPMSQ and SGDS. Conclusion : The prevalencies of cognitive impairment and depressive symptomatology were high in institutionalized elderly people. This results suggests that more intensive mental health care is needed for long-term institutionalized elderly people.

200番체 通過率이 다짐效果에 미치는 影響

趙誠正,閔興基 충북대학교 농업과학기술연구소 1991 農業科學硏究 Vol.9 No.2

This study was conducted to investigate on the influence of the passing percent of No. 200 sieve on the compaction effect of adjusted soil sample, compared with the effect of natural soil sample, decreasing the passing percent of No. 200 sieve of natural soil sample. The results obtained were as follows ; 1.In the case of the passing percent of No. 200 sieve was much too small (A soil sample : 23%) or too large (B soil sample), though the passing percent of No. 200 sieve was decreased, the influence affecting the change of maximum dry density did not appear, but the optimum moisture content was decreased. 2.There Was a tendeney that the optimum moisture content was increased conversely, whereas the maximum dry density of adjusted soil sample was decreased.

딸기 와인 개발을 위한 최적 발효 조건

김민기, 박미성, 엄은경, 조은혜, 이종수 배재대학교 자연과학연구소 2016 自然科學論文集 Vol.27 No.1

Optimal fermentation condition for development of new strawberry wine were investigated. Commercial yeast, Fermivin was good for brewing of strawberry wine and its optimal addition concentration was also 1%. Maximal ethanol content of strawberry wine was obtained when raw strawberry juice(24 brix) was fermented by 1% Fermivin at 25℃ for 7 days and its antioxidant activity was 78.5%. 새로운 딸기 와인을 개발하기 위한 최적 발효조건을 연구하였다. 시판효모인 페미빈이 딸기와인 제조에 제일 좋았고 1%가 최적 첨가 농도이었다. 딸기와인의 최대 에탄올 생성량은 생딸기주스 (24brix)를 1% 페미빈으로 25℃에서 7일 발효시켰을 때 얻어졌고 이 발효액의 항산화활성은 78.5% 이었다.

솔잎(Pinus densifloras Z.)의 조성분이 전통 약주 발효에 미치는 영향

조남지,조민자,조규성 안성산업대학교 1995 論文集 Vol.27 No.-

藥酒 製造시 솔잎 添加 效果를 調査하기 위하여 솔잎 抽出物 첨가가 S. cerevisiae의 生育에 미치는 影響과, 솔잎분말을 添加한 제국 과정 중의 酵素 活性 및 약주 발효 과정 중 pH, 總酸, 알코올의 含量 變化를 調査하였다. 솔잎의 일반 성분으로는 수분 54.80%, 단백질 8.18%, 조지방 2.07%, 섬유 23.13%, 조회분 1.10%였다. 솔잎 첨가구가 대조구에 비하여 S.cerevisiae의 증식을 억제하는 효과가 있었다. 제국 과정 중의 amylase 활성은 모든 시험구에서 제국 24시간에서 최고의 활성을 나타내었고, 솔잎 2.0%구는 대조구에 비하여 높은 활성을 보였다. 산성 protease 활성은 솔잎 첨가구가 대조구에 비하여 효소활성이 높았으며, 솔잎 분말 2.0% 첨가구는 발효 24시간에서 활성이 가장 높았다. pH는 솔잎의 첨가량이 증가할수록 높았으나 발효기간이 경과할수록 감소하였다. 총산의 변화는 발효기간이 길어질수록 높았고, 0.1, 0.5, 1.0% 솔잎첨가구는 대조구에 비하여 총산의 함량이 높았으며 첨가량이 많아질수록 낮아졌다. 솔잎 분말의 첨가량이 증가할수록 알코올의 함량은 대조구보다 낮았으나, 첨가량에 따른 큰 영향은 나타나지 않았다. The study is to investigate the effects of addition of pine needles's extract on the growth of Saccharomyces cerevisiae and to investigate the changes of α-amylase activity, acid-protease activity, pH, total acidity, alcohol content during alcohol fermentation with variation of the amount of pine needles powder. The composition of pine needles sample were as follows : moisture 54.8%, crude protein 8.18%, crude lipid 2.07%, crude fiber 23.13%, crude ash 1.10%. Treatment groups with pine needles extract have an inhibitory effect on the growth of S. cerevisiae companre to control group. The highest activity of α-amylase was shown at 24 hours fermentation in all treatments and the only treated group with 2.0% addition of pine needles powder had higher amylase activity than that of control group. The activity of acid-protease in the treatments was higher than that of controls and the highest protease activity of 2.0% treated group was shown at 24 hours fermentation. The pH was increased in the adding more pine needles powder but it would be decreased with the lapse of fermentation time. The change of total acidity was increased with the longer fermentation. Although the content of total acidity was higher in 0.1, 0.5 and 1.0% treated groups than that of control group, the small in addition of pine needles powder the less total acid content. Although the alcohol content of treated groups were a little lower than that of control group, there were no different effect on the alcohol content as the additive amount of pine needles powder.

SOL-GEL법을 이용한 강유전체 BaTiO₃박막 및 Ceramics 제조

조채룡,이수재,장민수,김호종,정세영,노동택,김성철 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

Concentrated solution was prepared for a sol-gel process of BaTiO₃. Barium hydroxide and Ti-isopropoxide were used as the raw materials with methanol and isopropanol as solvents. Dense ferroelectric polycrystalline BaTiO₃thin films with the perovskite structure were fabricated by spin coating of the prepared concentrated solution on a few substrates. From XRD investigation, it was confirmed that tetragonal phase of polycrystalline BaTiO₃powders are formed after heat treatment above 550℃ in air and grain-oriented BaTiO₃thin films are obtained by coating of 0.06M concentrated solution on the substrates. It was observed that surfaces of thin films deposited on the substrates by our methods were formed crack-free and uniformly. The thickness of thin film which depends the concentration of the solution was 400∼900Å for 1 time coating, which was confirmed by SEM analysis. Ceramics and thin films show dielectric constant of 4600 (at 1㎑) and capacitance 300∼800㎊ (at 1㎑) at room temperature, respectively.

생체분해성 고분자를 이용한 사시수술용 제형개발 및 효용성에 대한 연구

민병무,김용백,김승영,김창식,박근성,길숙종,조항진,이성복,노승무,송규상,강대영,조준식,양준묵,정경수,최선웅,이진호,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

A new device, muscle clamping system was developed to facilitate exact quatifying technique A strabismus surgery, and reduce the risk of complications. The device is composed of a lower fixing body with three jaws and an upper supporting body. They are used to clip an extraocular muscle and fix it to the sclera with a single bite. Superior rectus recession on 16 rabbit eyes were performed with this new device. Conjunctival injection, muscle adhesion strength, and light microscopic findings were examined at 1, 2, 4 and 8 weeks postoperatively. The Conjunctival injection were minimal, adhesion power ranged from 420 to 600 gram gravity, which is sufficient in withstand the normal pull of human extraocular muscle. In microscopic exam, some Inflammatory cells and fibrosis were found. The new device was technically easy, fast, and accurate, so it may be useful in stabismus surgery.

시험도로 연속철근콘크리트 포장에서의 수평균열에 관한 연구

조병위,김성민 慶熙大學校 大學院 院友會 2005 高凰論集 Vol.37 No.-

콘크리트 슬레브 증간깊이에 발생한 수평균열에 의한 스폴링, 펀치아웃같은 손상들이 연속철근콘크리트 포장(CRCP)에서 관찰되었다. 수평균열의 원인을 밝혀내기 위해서 현장실험과 더불어 수치해석을 수행하였으더 코어를 채취하여 콘크리트 슬래브 내에 수평균열의 분포를 조사하였다. 수평균열의 원인들을 파악하기 위해 설계, 재료 및 환경에 관련된 다양한 변수들을 현장 실험 및 수치해석시 고려하였고 CRCP의 수치해석 모델은 유한요소법을 사용하며 개발하였으며, 이 모델에 의해 소사된 CRCP내의 전단 및 인장응력 분포로 현장실험으로부터 얻은 결과들을 확인하였다. 횡방향 근열부근에서 슬래브 중간깊이에 위치한 철근부근에서 가장 높은 전단 및 인장응력이 발생함을 수치해석 결과를 통해 확인하였다. 수평균열이 할생한 단면과 그렇지 않은 단면의 란성계수와 열팽창계수같은 현장타설 콘크리트의 물성치 및 시공단계에서의 환경 조건들에 대한 평가가 수치해석결과가 타당함을 됫받침해준다. Severe spalling and half-depth punchout failures associated with horizontal cracks at the mid-depth of concrete slabs were observed in continuously reinforced concrete pavement (CRCP). To identify the causes of those horizontal cracks, field observations, laboratory tests, and numerical analyses were conducted. The extent of horizontal cracking in the concrete slab was investigated with coring. Several variables relative to design, material, and environment were considered in the field and laboratory studies to evaluate possible causes of horizontal cracking. Numerical models of CRCP were developed using the finite element discretization and the shear and normal tensile stress distributions in CROP were investigated with the models to verify the results obtained from the field and laboratory studies. Numerical analysis results show that maximum shear and normal tensile stresses develop near the depth of steel bars at transverse cracks. Evaluations of in-situ concrete material properties, such as modulus of elasticity and thermal expansion coefficient, from sections with and without horizontal cracks and environmental conditions during construction all support the results of the numerical analysis.