Molecular Genotyping of Diverse Bacteria Using Repetitive DNA sequence-based PCR

Kim,K.-S.,Oh,C.-Y.,Seo,H.-A. 中央大學校 遺傳工學硏究所 1999 遺傳工學硏究論集 Vol.12 No.1

ERIC(enterobacterial repetitive intergenic consensus) 염기서열과 REP(repetitive extragenic palindromic) 염기서열의 생물학적 기능과 다양한 세균 균주들의 분자생물학적 동정에의 응용에 관하여 고찰하였다. ERIC 혹은 REP 염기서열은 염기서열 그 자체는 진화론적으로 상당히 보존되었으나 그들의 염색체상의 위치는 세균 종마다 다르다. 그러므로, ERIC 혹은 REP 염기서열과 상보적인 PCR용 primer를 고안하여 연쇄중합 반응을 시키면, ERIC 혹은 REP 염기서열 사이로부터 증폭된 크기가 다른 PCR 생산 DNA단편들이 만들어진다. 이때 증폭된 크기가 다른 DNA 단편들을 이용하여 각 세균 균주마다 고유한 DNA fingerprinting pattern이 결정될 수 있다. 그렇게 결정된 pattern들을 서로 비교하여 검체 균주간의 상보성과 유전학적 연관성 등이 결정될 수 있다. 그러므로 ERIC 혹은 REP 염기서열을 이용한 PCR반응은 식품으로부터 분리될 수 있는 다양한 세균 균주의 동정에 이용될 수 있는 최근에 개발된 새로운 분자생물학적 기법이다. A family of interspersed repetitive DNA elements in prokaryotic genomes, emphasizing on enterobacterial repetitive intergenic consensus (ERIC) sequences and repetitive extragenic palindromic(REP) sequences, have been discussed in terms of their biological functions and their applications on genotyping diverse bacterial strains. ERIC or REP sequences are highly conserved at the nucleotide sequence level, but their chromosomal locations differ between species. Several features of ERIC sequences resemble those of REP sequences although the nucleotide sequence is entirely different. ERIC or REP sequences can be used as oligonucleotide primer binding sites for polymerase chain reaction (PCR)-mediated genomic fingerprinting. By using outwardly facing oligonucleotide primers complementary to ERIC or REP sequences. PCR reactions can amplify differently sized DNA fragments consisting of sequences lying between elements. Consequently, the multiple amplicons of different sizes can be used to establish DNA fingerprinting patterns specific for individual bacterial isolates. The established patterns can be compared to estimate relative degrees of similarity between isolates and to determine whether isolates are clonally related. Therefore, the ERIC or REP sequence-based PCR fingerprinting can be a powerful tool to discriminate diverse foodborne bacterial isolates from each other.

Exploring Challenges and Strategies in Promoting Korean Culture through Museum Exhibition in the United States

Seoha Min,진병호 한국학중앙연구원 한국학중앙연구원 2017 Korea Journal Vol.57 No.2

A number of countries have attempted to promote their national image to increase global competitiveness. As one way of promoting the national image, we have focused on the museum venue, which significantly facilitates the experience of new cultures. This study explores the challenges and strategies in promoting Korean culture through museum exhibitions in the United States. By examining the presentation of Korean culture through museum exhibitions in the United States, this research will benefit other countries striving to promote their national images by maximizing available cultural resources. To achieve its research purposes, this study conducted two phases: (1) we counted, categorized, and analyzed Korean cultural objects exhibited by representative museums online to understand how Korean culture has been presented; and (2) interviews were conducted of curators currently involved with Korean art to explore challenges they have experienced in promoting Korean cultural objects via museum exhibitions. Based on the collected data, we were able to identify several themes regarding the challenges and promotional strategies of museums exhibiting Korean cultural objects. The findings of this study will provide curators and museums with practical insights into promoting Korean culture through museum exhibitions.

Gendered role communication in marketing blue-collar occupational gear and clothing in the United States

Seoha Min 한국의류학회 2015 Fashion and Textiles Vol.2 No.1

Considering the need for gender equity in the workforce, this research explores social construction of gender; how that construction relates to work; and, subsequently, how gender construction influences the design and marketing of work clothing and personal protective equipment (PPE) for women working in blue-collar occupations. To explore the phenomenon, a content analysis of products and advertising images in seven websites selling blue-collar work clothes and PPE was used. Collected data suggests work clothing and PPE designed for women is limited in terms of size, design, and number of products available. It was also found that there exists a cultural discomfort with portraying women as competent blue-collar professionals in the advertisement of work clothing. The research provides a discussion of guiding ideas for improvement in the design and marketing of products for women workers in blue-collar occupations. Findings suggest design and marketing images could be improved to better reflect and appeal to women purchasing and using these products.

Role power dynamics within the bridal gown selection process

Seoha Min,Lina M. Ceballos,Jennifer Yurchisin 한국의류학회 2018 Fashion and Textiles Vol.5 No.1

In this study, the researchers explore the decision-making process of brides when selecting their wedding dresses by examining the power of the significant people involved in the selection process. Within a framework of role theory and symbolic interactionism, qualitative data from an open-ended survey with 71 brides were collected online and analyzed. The interpretations revealed two main themes that explain how social power is determined and how different actors use bases of power to influence the selection of the wedding dress, when considered as a group decision. The six main categories further explained the bases of power used by the actors involved in the wedding dress selection process. This research extends the understanding of the bases of power and social roles in the specific context of the ritual of matrimony. Managerial implications are included for wedding retailers seeking to further understand the dynamics among family members during wedding preparations, so they can provide more effective guidance and support to the bride-to-be during the selling process.

A 24-hour acute fungicide ecotoxicity assessment method based on yeast (Kazachstania barnettii) growth

Seoha Lee,Sohyun Lee,Haneur Lee,Jihae Park,Taejun Han,Hojun Lee 환경독성보건학회 2023 한국독성학회 심포지움 및 학술발표회 Vol.2023 No.11

해수를 활용한 나트륨기반 전기화학적 에너지저장 및 자원화 기술

김서해(Seohae Kim),진효(Hyo Jin),이왕근(Wang-Geun Lee) 한국신재생에너지학회 2023 한국신재생에너지학회 학술대회논문집 Vol.2023 No.6

Effect of X-ray Irradiation on Developmental Stages of Drosophila suzukii (Diptera: Drosophilidae)

Jeongmin Kim,Seohae Lee,Junheon Kim,Chung Gyoo Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Spotted wing drosophila (SWD), Drosophila suzukii, is one of the most harmful pests causing serious damages to blueberries in Northern America and Europe. It is essential to seek alternatives to methyl bromide to ensure dis-infestation of this pest from agricultural products. We investigated the effect of X-ray irradiation (0, 50, 100, 200 and 300 Gy) on development and reproduction of SWD to meet this standard. When eggs were irradiated with the doses, some portion of the eggs hatched even at 300 Gy. The larvae hatched from the irradiated eggs did not pupate at 300 Gy, and they could not develop to adults even at 50 Gy. When larvae were irradiated, they developed up to pupa in some proportion. However above 100 Gy, there was no adult emergence. When pupae were irradiated, some of them emerged to adults. However, the adults could not produce eggs at all above 100 Gy. When adults were irradiated, oviposition occurred normally, but fecundity and hatchability were decreased as irradiation dose went up.

Effect of Gamma Ray Irradiation on the Development of Drosophila suzukii (Diptera: Drosophilidae)

Jeongmin Kim,Seohae Lee,Junheon Kim,Chung Gyoo Park 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

Spotted wing drosophila, Drosophila suzukii, native to eastern and southeastern Asia, has become one of the most harmful pests causing serious damage to fruits such as blueberry, cherry, and others in Northern America and Europe. For quarantine sanitary purpose, methyl bromide (MeBr) has been used for a long time. However, it is under regulation because of its adverse effect of ozone layer depletion. Thus, alternative strategies to MeBr are needed to meet the quarantine requirement. In this study, we investigated effects of gamma ray irradiation (0, 50, 100, 200 and 300 Gy) on the development and reproduction of D. suzukii to offer a guideline in using gamma ray for dis-infestation of this pest. The higher doses of gamma ray caused more adverse effects on development and hatchability of D. suzukii. Development and oviposition of D. suzukii was significantly depressed at above certain doses, respective to each developmental stage. When eggs were treated with the doses of gamma ray, eggs hatched in some ratios at all doses. Even though some larvae developed to pupae under 100 Gy irradiation, all these pupae did not emerge to adults at all doses. When larvae were treated with gamma ray, some of them pupated at each doses. However these pupae did not emerge to adults at all under all doses. When pupae were irradiated, the emerged adults did not oviposit eggs above 100 Gy. When adults were irradiated, oviposition occurred normally, but fecundity and hatchability of the eggs were reciprocal to the doses.

‘중형선고’ 논란에 따른 형벌포퓰리즘에 대한 실증 연구 : 비정형 빅데이터 분석방법론의 적용

최우철 ( Woochul Choi ),박서하 ( Seoha Park ),노승국 ( Seungkook Roh ) 대한범죄학회 2021 한국범죄학 Vol.15 No.1

2020년 세상에 드러났던 n번방 사건은 범행의 잔혹성으로 국민의 공분을 샀다. 하지만 n번방 사건 관련 인터넷 기사의 댓글에서 누리꾼들이 범죄에 대한 분노나 피해자에 대해 안타까움을 나타내는 경우도 많았지만, 시작되지도 않은 범죄자들에 대한 재판에 대해 가벼운 형이 선고될 것이라며 우려를 나타내는 경향 또한 확인할 수 있었다. 이와 같은 사법부 판결에 대한 적대적 여론은 n번방 사건과 관련된 기사에서 처음 나타난 것이 아니라 다른 많은 범죄 관련 기사에서도 일상적으로 확인할 수 있었다. 더 높은 형량을 요구하는 이런 댓글들이 어떻게 등장했는지 살피기 위해, 본 연구는 20년간의 “중형 선고” 관련 기사와 댓글을 분석해 엄벌주의와 형벌 포퓰리즘 이론의 적용 가능성을 검토했다. 엄벌주의는 하나의 정립된 이론으로써 그 내용이 비교적 명확하지만, 형벌 포퓰리즘 이론은 최근 제시된 새로운 견해에 대해 정치권으로부터 형성되는 경향인지 국민으로부터 요구되는 경향인지에 대한 실증적 연구가 부재했다. 분석 결과 실제로 엄벌주의 경향과 국민적 요구의 형벌 포퓰리즘이 나타난다면 사법부 판결에 대한 불만의 해소책 마련 또한 필요했다. 데이터 분석 결과는 다음과 같았다. 기사에 대한 분석에서는 언론에서 주로 다뤄지는 중형 선고 관련 범죄의 유형과, 선고에서 나타난 형량의 변화를 알 수 있었는데, 부정적 여론과 징역형 최고형의 상향에도 불구하고 전반적인 형량의 분포는 변하지 않았고, 몇몇 극단값만 추가되었음을 확인할 수 있었다. 댓글에 대한 분석에서는 기사의 내용과 비교해 사람들이 관심을 많이 가진 범죄유형에 대해 알 수 있었고, 중형 선고와 관련된 많은 댓글에서 사법처리와 관련된 단어가 부정적인 맥락에서 쓰였음을 확인할 수 있었다. 또한 사형, 무기의 빈도가 매우 높게 나타나 강한 처벌을 바라는 경향을 확인 할 수 있었다. 본 연구에서는 이에 대해 국민적 형벌 포퓰리즘이 나타나고 있음을 확인하고, 형벌의 목적 면에서의 분석을 통해 기 현상의 원인과 대응책에 대해 논한다. The “Nth Room” case, which was revealed at 2020, cause public outrage for its brutality. However, comments from Internet articles featuring the case showed that Internet users often expressed anger over the crime or sorrow over the victim, but they also expressed concern that the trial for criminals which didn’t even start will give them light sentences. This hostile public opinion on the judicial decisions was not first seen in articles related to the case, but was routinely identified in many other criminal-related articles. To examine how these comments demanding higher senteces appeared, this study analyzed articles and their comments related to “harsh sentencing” over the period of 20 years to examine the applicability of Punitiveness and Penal Populism. While Punitiveness is a well established theory and its contents are relatively clear, there has been no empirical study of the recent proposed new perspective of Penal Populism about whether it is a tendency formed by politicians or a demand from the people. If the analysis actually shows a tendency to Punitiveness and Penal Populism, it is also necessary to come up with measures to resolve complaints about the judicial decisions. The results of the data analysis were as follows. The analysis of the news articles showed the type of crime mainly dealt with in the media and the change in the sentencing, which indicated that the overall distribution of sentences remained unchanged but only a few extreme sentences were added despite negative public opinion and demand of harsher sentences. The analysis of the comments showed the types of crimes people were interested in compared to the content fo the artile, and many comments related to the Punitiveness confirmed that the words related to judical processing were used in a negative context. In addition, the frequency of the word “death penalty” and “life-time” was very high, indicating the tendency to seek strong punishment. In this study, we confirm that people-demanded Penal Populism is emerging and discus te causes and countermeasures of pheomena through analysis in terms of the purpose of punishment.

Development and Evaluation of a Patient Garment Based on Functional-Modesty-Aesthetic (FMA) Model

Helen S. Koo,Seoha Min 한국복식학회 2014 International journal of costume and fashion Vol.14 No.2

Recent research suggested that hospitals have not responded to consumer demand for the improved aesthetic and comfort of their patient garments. Thus a new patient garment has been developed considering human factors based on the Functional-Modesty-Aesthetics model. Based on the reviewed literature and the model, necessary design requirements were identified. Then the patient garment was evaluated in comparison to existing ones with regard to potential patients’ satisfaction levels. As a result, the developed patient garment received better evaluations in the aspects of overall aesthetic satisfaction, color satisfaction, and willingness to wear than the existing patient garments.