The Prognostic Significance of the Overexpression of HER-2/ neu in Korean Gastric Carcinomas and the In Vitro Effects of Anti-HER-2/neu Antibody on Cell Growth in the Gastric Carcinoma Cell Lines

Seock-Ah Im,Kyung Eun Lee,Eunmi Nam,Seung Hyun Nam,Do Yeun Kim,Chu-Myong Seong,Hae-Young Park,Woon Sup Han,Ju-Young Seoh,Soon Nam Lee 대한암학회 2003 Cancer Research and Treatment Vol.35 No.2

학령기 아동의 아토피 피부염 예방 및 관리를 위한 영양교육 프로그램 개발 및 적용

강석아 ( Seock Ah Kang ),최화정 ( Hwa Jung Choi ),김정현 ( Jung Hyun Kim ) 한국식품영양학회 2012 韓國食品營養學會誌 Vol.25 No.2

초등학교 3학년 아동을 대상으로 아토피 피부염 예방과 관리를 위한 영양교육을 실시하였다. 3학년 총 6개 반 중 3개반은 영양교육군(교육군), 나머지 3개 반은 비교육군(대조군)으로 선정하여 전체를 대상으로 영양교육(전) 설문조사를 실시하고, 실험군 97명을 대상으로 약 6주 동안 영양교육을 실시한 후 3학년 전체를 대상으로 영양교육(후) 설문조사를 실시하고, 총 191명을 대상으로 분석을 실시하여 영양교육 효과를 평가하였다. 본 연구의 목적은 아토피 예방 및 관리를 위한 영양교육을 통하여 잘못된 식습관을 교정하고, 올바른 식습관을 형성을 토대로 식행동 변화를 도모하여 궁극적으로 아토피를 이기는 식생활을 실천할 수 있도록 하고자 하는것으로, 아토피 피부염을 예방하고 관리하기 위한 방안으로써 영양교육의 필요성을 제시하고자 한다. 영양교육은 총 6차시로 진행되었으며, 각 차시별 교육내용을 살펴보면 1ㆍ2차시는 식품첨가물의 유해성을, 3차시는 설탕(당)의 유해성을, 4차시는 아토피와 편식과의 관계를 이해하고, 5차시는 아토피 관련 식생활 정보를 습득하고, 6차시에는 제철(교육 당시 7월) 과일을 이용하여 오미자 화채를 만드는 실습을 하였다. 식생활 태도 조사 결과, 영양교육 전ㆍ후에 따라 대조군의 경우는 식생활 태도 점수가 평균 3.98(SD=0.59)에서 사후 평균 3.99(SD=0.71)점으로 나타나 변화를 보이지 않았으나, 교육군에서는 아토피 피부염 관련 영양교육 후 식생활 태도 점수가 평균 3.84(SD=0.59)에서 평균 4.16(SD=0.65)으로 유의적으로 증가한 것으로 조사되었다. 영양교육 프로그램 실시전ㆍ후에 두 집단 사이 그리고 집단 내에 유의한 차이가 있는 지를 조사하기 위해 각각 paired t-test를 실시한 결과, 대조군의 경우 전ㆍ후 차이가 없었고, 교육군의 경우는 전ㆍ후 유의한 차이가 있는 것으로 나타났다. 간식행동(간식 먹는 횟수, 섭취 이유, 간식 종류, 간식 섭취 장소, 간식 구매 시 고려사항)에 대해 조사한 결과, 아토피 피부염 관련 교육을 받은 교육군의 경우, 간식 종류로 집에서 만들어 주는 간식이 40.2%에서 57.7%로 17.5%가 증가하였고, 간식을 섭취하는 장소가 집으로 응답한 비율이 68%에서 81.4%로 13.4%가 증가하였다. 주변동질성검정을 통하여 대조군과 교육군의 각 항목별 아토피 피부염 관련 영양교육 프로그램 적용 후 사전-사후 검사에 대한 차이를 살펴본 결과, 교육군의 경우 교육군에서 아이스크림, 빵류, 초콜릿류 등에서 통계적으로 유의하게 섭취 빈도가 감소함을 보였으며(p<0.01), 스넥류, 사탕 & 캐러멜류, 스포츠 & 이온음료류도 통계적으로 유의하게 섭취 빈도가 감소함을 보였다(p<0.05). 대조군과 교육군의 간식 섭취 빈도에 관한 사전 조사 결과는 통계적으로 유의한 차이를 보이지 않았으나, 교육군에게 영양교육을 적용 후 사후 검사를 한 결과에서는 초콜릿류의 간식 섭취 빈도가 통계적으로 유의하게 감소한 것을 알 수 있었다. 아토피 피부염 관련 영양교육 프로그램을 적용한 결과, 교육군에서 간식 종류에 따라 선호도의 유의적인 감소를 보였다. 즉, 튀김류, 스넥류, 초콜릿류에서 통계적으로 유의한 선호도의 감소를 보였으며(p<0.05), 빵의 경우도 통계적으로 유의한 선호도가 감소된 것을 알 수 있었다(p<0.01). 이상의 연구는 아토피 피부염 관련 영양교육 프로그램을 적용하여 사전-사후 검사를 한 결과, 전반적인 식생활 태도는 물론 아토피 피부염에 직접적으로 관련이 있는 간식행동(간식 섭취 장소, 간식 종류, 간식섭취 빈도)과 간식의 종류별에따른 선호도에 유의적인 변화를 보였다. 즉, 아토피 피부염의 예방 및 관리에 도움이 될 수 있도록 긍정적인 방향으로 개선되었음을 알 수 있었다. 그러므로 아토피 피부염 예방과 관리를 위한 영양교육의 효과가 있는 것으로 판단되며, 보다 실효성 있는 교육 효과를 위해서는 교육기회의 확대와 아토피 관련 정책 연구 시 교육현장에서 활용할 수 있는 다양한 영양교육 자료 개발이 요구된다. Atopic related skin symptoms must be controlled continually. Because it is involved with food, eating behaviors play an important role in the prevention and management of atopic dermatitis. The aim of this study was to develop and apply nutrition education program for the prevention and management of atopic dermatitis on elementary school students. After applying the nutrition education program, our research attempts to evaluate the effectiveness of such nutrition education program. This study examined the effects of a nutrition education program on atopic dermatitis-related snacking behaviors, eating attitudes, and food behaviors. Subjects were 3rd grade elementary school students in Daejeon. A 6-week nutrition education program was implemented to 97 children as the educated group and another 94 children were included in the study as the control group. A self-administered questionnaire was used to assess the effects of nutrition education program. As results, eating attitudes reached significant improvement by the nutrition education program(p<0.05). In a paired t-test, the control group did not show significant difference, but the educated group showed a significant difference. In snacking behaviors, the two groups did not show statistical difference except for some considering factors. Also, there were no significant differences of the two groups after nutrition education program, except the snacking place after the nutrition education group. However, in the educated group, the types of snacks made by mothers increased 17.5% from 40.2% to 57.7%, and in-house as snacking place increased 13.4% from 68% to 81.4%. In the results of before and after the nutrition education program, the program showed some effect on the prevention and management of atopic dermatitis. Therefore, these results showed foundation for nutritional education and various educational programs are needed afterward to increase the effect of nutrition education in the prevention and management policy related to atopic dermatitis.

본태성고혈압 환자에서의 Doppler 심초음파 소견에 대한 연구

임석아(Seock Ah Im),정혜경(Hye Kyung Jung),정성애(Sung Ae Jung),박정은(Jung En Park),신길자(Gil Ja Shin),이우형(Woo Hyung Lee) 대한내과학회 1995 대한내과학회지 Vol.48 No.1

N/A Objectives: Systemic arterial hypertension causes structural and functional changes of myocardium and coronary circulation, increased tendency to ventricular arrhythmia, ischemic heart disease, and congestive heart failure. In recent years, the echocardiogram has been developed as noninvasive method for the evaluation of left ventricular performance. Estimation of systolic and diastolic function of left ventricle and measurement of left ventricular mass may predict outcome of hypertensive patients. Methods: We measured systolic and diastolic function of left ventricle and left ventricular mass with M-mode, two-dimensional, and Doppler echocardiography in 62 cases of hypertensives and 20 cases of healthy adults from November 1992 to August 1993. Results: 1) Hypertensive group was 62 cases (M:F=33:29), the mean age was 56±13 years. Normotensive group was 20 cases (M:F=8:12), the mean age was 53±10 years. There was no significant differences in age distribution, sex ratio, height, weight, and body mass index. 2) On M-mode echocardiographic measurements, interventricular septum thickness and LV posterior wall thickness were significantly increased in hypertensive group (14.4±2.9㎜, 13.9±2.9㎜) than normotensive group (10.7±1.1㎜, 10.3±1.6㎜) (p<0.01). 3) Ejection fraction was decreased in hypertensive group (55.8±12%) than normotensive group (63.0±6.7%) but within normal range. 4) There was no significant differences of cardiac output and cardiac index between hypertensive group (4.69±1.151/min., 3.20±2.641/min./㎡) and normotensive group (4.88±1.211/min., 3.02±0.791/min./㎡). 5) There was no significant differences of peak E velocity between hypertensive group (72.8±22.4㎝/sec) and normotensive group (77.1±16.3㎝/sec). Peak A velocity was significantly increased in hypertensive group (83.1±22.2㎝/sec) than normotensive group (62.6±15.0㎝/sec) (p<0.01). Peak E/A ratio was significantly decreased in hypertensive group (0.91±0.28) than normotensive group (1.22±0.25) (p<0.01). Mitral valve deceleration time was significantly prolonged in hypertensive group (240.0±48.4msec) than normotensive group (240.0±48.4msec) (p<0.01). Conclusion: Diastolic dysfunction and left ventricular hypertrophy were prominent in hypertensive group but systolic function was relatively saved.

Src as a Therapeutic Target in Biliary Tract Cancer

Nam, Ah-Rong,Kim, Ji-Won,Park, Ji Eun,Bang, Ju-Hee,Jin, Mei Hua,Lee, Kyung-Hun,Kim, Tae-Yong,Han, Sae-Won,Im, Seock-Ah,Kim, Tae-You,Oh, Do-Youn,Bang, Yung-Jue American Association for Cancer Research 2016 Molecular Cancer Therapeutics Vol.15 No.7

<P>Src, a nonreceptor tyrosine kinase, is involved in a number of cancer-related signaling pathways and aberrantly activated in biliary tract cancer (BTC). This study aimed to elucidate the potential role of Src as a therapeutic target in BTC. We tested bosutinib, an orally active c-Src/Abl kinase inhibitor, alone or in combination with cytotoxic agents using 9 human BTC cell lines: SNU-245, SNU-308, SNU-478, SNU-869, SNU-1079, SNU-1196, HuCCT1, TFK-1, and EGI-1. Of these, SNU-308 and SNU-478 were relatively sensitive to bosutinib. Bosutinib abrogated phosphorylation of Src and its downstream molecules, and significantly increased G(1) cell-cycle arrest and apoptosis. Bosutinib significantly inhibited cell migration and invasion and decreased epithelial-mesenchymal transition markers. Bosutinib combined with gemcitabine or cisplatin showed synergistic antiproliferative and antimigratory effects. In addition, this combination further inhibited phosphorylation of Src and its downstream molecules and decreased epithelial-mesenchymal transition marker expression compared with bosutinib alone. We established a SNU-478 xenograft model for in vivo experiments, because SNU-478 was more tumorigenic than SNU-308. Bosutinib combined with gemcitabine or cisplatin showed significantly more potent antitumor effects than bosutinib alone. Bosutinib combined with gemcitabine further decreased Ki-67 expression and Src phosphorylation, and further increased TUNEL expression. Our data suggest that Src might be a potential therapeutic target in BTC. Bosutinib demonstrated promising antitumor activity alone or in combination with gemcitabine or cisplatin in BTC cells, which supports further clinical development in patients with advanced BTC. (C) 2016 AACR.</P>

Antiangiogenesis Treatment for Gliomas: Transfer of Antisense - Vascular Endothelial Growth Factor Inhibits Tumor Growth in Vivo

Im, Seock-Ah,Mazano, Cankelaria Gomez,Fueyo, Juan,Liu, Ta-Jen,Ke, Li-Dao,Jeong Soo Kim,Lee, Ho-Young,Steck, Peter A.,Kyritsis, Athanassios P.,Yung, W.K. Alfred 가톨릭대학교 2000 Bulletin of The Catholic Research Institutes of Me Vol.28 No.-

Presently, there is no effective treatment for glioblastoma, the most malignant and common brain tumor. Angiogenic factors are potentially optimal targets for therapeutic strategies because they are essential for tumor growth and progression. In this study, we sought a strategy for efficiently delivering and antisinse cDNA molecule of the vascular endothelial growth factor(VEGF) to glioma cells. The recombinant adenoviral vector Ad5CMV-αVEGF carried the coding sequence of wild-type VEGF165 cDNA in an antisense orientation. Infection of U-87 MG malignant glioma cells with the Ad5CMV-αVEGF resulted in reduction of the level of the endogenous VEGF mRNA and drastically decreased the production of the targeted secretory form of the VEGF protein. Treatment of human glioma tumors established in nude mice with intralesional injection of Ad5CMV-αVEGF inhibited tumor growth. Taken together, these findings indicate that the efficient down-regulation of the VEGF produced by tumoral cells using antisense strategies has an antitumor effect in vivo, This is the first time that an adenoviral vector is used to transfer antisense VEGF sequence into glioma cells in an animal model, and our results suggest that this system may have a clinical and therapeutic utility. (Cancer Researcg 59:895-900, 1999)

Phase II trial of preoperative paclitaxel, gemcitabine, and trastuzumab combination therapy in HER2 positive stage II/III breast cancer: The Korean Cancer Study Group BR 07-01

Im, Seock-Ah,Lee, Keun Seok,Ro, Jungsil,Lee, Eun Sook,Kwon, Youngmee,Ahn, Jin-Hee,Ahn, Jin Seok,Kim, Jee Hyun,Kang, Han Sung,Shin, Kyung Hwan,Noh, Dong-Young,Park, In-Ae,Kim, Sung-Bae,Im, Young Hyuck Springer-Verlag 2012 Breast cancer research and treatment Vol.132 No.2

가토의 망막 색소상피 세포내 Acid Phosphatase의 분포 및 활성

석귀덕,서영아 대구효성가톨릭대학교 자연과학연구소 1990 基礎科學硏究論集 Vol.4 No.-

Lysosomal enzyme activity in the retinal pigment epithelium (RPE) of rabbit eyes was measured using acid phosphatase(ACPase) as an enzyme marker. Distributions of ACPase in RPE were measured by standard enzymological techniques. The REP and choroidaltissues were first homogenated and then centrifuged. Sediments were RPE which wasconfirmed electron microscopically. In the subcellular fractions of RPE, the specific activity of the ACPase was most Prominent in the Iysosomal fraction (80.15± 26.82?g p-nitrophe-nol/mg protein by Fukuzawa method). The nuclear (31.60± 19.38), mitochondrial (27.87± 18.23) and soluble fraction (53.63± 21.70) also showed the specific of the enzyme.The preparation of microsomal fraction showed a trace of the specific acticity of the engyme.

An Efficient Synthesis of an Apoptosis Inducer, F-3-2-5 by Using Octanol-Accelerated Baylis-Hillman Reaction

Kang, Seock-Yong,Park, Kwang-Su,Kim, Jin-Young,Chong, You-Hoon,Choo, Hyun-Ah Korean Chemical Society 2007 Bulletin of the Korean Chemical Society Vol.28 No.2

Immediate Effects of Soft Tissue Massage on Posterior Shoulder Muscle Tightness: A Preliminary Study

( Sil Ah Choi ),( Ji Hyun Lee ),( Tae Lim Yoon ),( Heon Seock Cynn ) 한국전문물리치료학회 2012 한국전문물리치료학회지 Vol.19 No.4

Posterior shoulder muscle tightness is frequently observed in shoulder impingement syndrome because tightness in the posterior portion of the shoulder muscles can cause anterior and superior translation of the humeral head in relation to the glenoid fossa. The purpose of this study was to determine the immediate effects of soft tissue massage on acromiohumeral distance (AHD), anterior translation of the humeral head, and glenohumeral (GH) range of motion (ROM) in subjects with posterior shoulder muscle tightness. Twenty-seven subjects with greater than 10° difference in the range of GH horizontal adduction between right and left sides were recruited. The range of GH horizontal adduction and internal rotation were measured by a digital inclinometer. The AHD and anterior translation of the humeral head were measured using ultrasonography. A paired t-test was used to compare AHD, anterior translation of the humeral head, and the range of GH horizontal adduction and internal rotation before and after soft tissue massage. The results showed that AHD increased significantly (p<.05) and the anterior translation of humeral head decreased slightly, but not significantly (p=.40) after the soft tissue massage. Furthermore, the ROM of horizontal adduction and internal rotation in the GH joint increased significantly after the soft tissue massage (p<.05). These findings indicate that soft tissue massage on posterior shoulder muscle tightness is an effective method to increase AHD and ROM in the horizontal adduction and internal rotation of the GH joint.

염색체 이상과 동반된 원발성 알도스테론증 환자의 8년 후 추적 관찰 1예 보고

임석아,남은미,박시훈,신길자,이우형,심봉석 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.1

8년전 양측성 부신증식에 의한 원발성 알토스테론증으로 진단받고 spironolactone 및 칼슘차단제를 투여하여 혈압이 조절되던 환제에서 최근 혈압이 조절되지 않아 전산화 단층 촬영을 제디행한 결과 부신선종이 동반된 것을 발견하고 부신 적출술 후 소량의 항고혈압제로 혈압이 조절되었던 1예를 보고하는 바이다. We describe an unusual 30-year-old female patient with a history of refractory hypertension and hypokalemia. She was diagnosed as primary aldosteronoism with bilateral adrenal hyperplasia 8 years age and blood pressure has been controlled with spironolactone 200mg/day, nifedipine 40mg/day, Cardura 4mg/day and oral potassium supplement till these days. Recently refractory high blood pressure was developed and about 5*4*4.5cm sized left a-drenal mass was observed by abdominal CT. The hypertension and hypokalemia was controlled by left adrenalectomy.