An Update on Contraception in Polycystic Ovary Syndrome

Seda Hanife Oguz,Bulent Okan Yildiz 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.2

Polycystic ovary syndrome (PCOS) is a common endocrine disorder in reproductive-aged women, characterized by hyperandrogenism, oligo/anovulation, and polycystic ovarian morphology. Combined oral contraceptives (COCs), along with lifestyle modifications, represent the first-line medical treatment for the long-term management of PCOS. Containing low doses of estrogen and different types of progestin, COCs restore menstrual cyclicity, improve hyperandrogenism, and provide additional benefits such as reducing the risk of endometrial cancer. However, potential cardiometabolic risk associated with these agents has been a concern. COCs increase the risk of venous thromboembolism (VTE), related both to the dose of estrogen and the type of progestin involved. Arterial thrombotic events related to COC use occur much less frequently, and usually not a concern for young patients. All patientsdiagnosed with PCOS should be carefully evaluated for cardiometabolic risk factors at baseline, before initiating a COC. Age, smoking, obesity, glucose intolerance or diabetes, hypertension, dyslipidemia, thrombophilia, and family history of VTE should be recorded. Patients should be re-assessed at consecutive visits, more closely if any baseline cardiometabolic risk factor is present. Individual risk assessment is the key in order to avoid unfavorable outcomes related to COC use in women with PCOS.

Emulsifier-Free Emulsion Polymerization of Methyl Methacrylate Containing Hydrophilic Magnetite Nanoparticles

Seda Beyaz,Taner Tanrisever,Hakan Kockar 한국고분자학회 2010 Macromolecular Research Vol.18 No.12

Emulsifier-free emulsion polymerization of methyl methacrylate was carried out in the presence of hydrophilic magnetite nanoparticles using a persulfate initiator. Before the magnetic nanoparticles were placed into the polymerization system, they were dispersed and stabilized electrostatically with perchloric acid in water (magnetic sol). For magnetic nanoparticles in the sol, the hydrodynamic radius and polydispersity index (PDI) measured by dynamic light scattering was 18.8 nm and PDI of 0.282, respectively. The latex sizes also were determined to be 125-223 nm with a PDI of 0.009-0.065. Magnetic measurements by vibrating sample magnetometry confirmed that the magnetite nanoparticles and magnetic poly(methyl methacrylate) nanospheres exhibited superparamagnetism with zero coercivity. The saturation magnetization of the magnetic polymeric nanospheres ranged from 0.2 to 1.19 emu/g depending on the magnetite content. The polymer chain terminations were affected by the addition of magnetite nanoparticles to the polymerization system. In addition, the magnetite that reduced the average molecular weight of the polymers obtained by gel permeation chromatography should be defined as a transfer agent.

Enhancing Citric Acid Production of Yarrowia lipolytica by Mutagenesis and Using Natural Media Containing Carrot Juice and Celery Byproducts

Seda Karasu Yalcin 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.3

In order to enhance citric acid production by Yarrowia lipolytica 57, mutation and selection treatments were performed by using UV-irradiation and/or ethyl methane sulfonate (EMS) as a chemical mutagen. Totally 18 mutant strains were demonstrated by testing of 1,234colonies and then used in citric acid production studies. Chemical mutagenesis was found as more effective in enhancing citric acid production than UV-induced mutagenesis. Maximum citric acid concentration (50.1 g/L) and yield obtained by the chemical mutant Y. lipolytica K-168 exceeded that of the initial strain by 57%. Growth and citric acid production of this strain was further examined in natural fermentation media containing carrot juice or celery byproducts. Maximum citric acid concentration reached to 62.6 g/L in diluted carrot juice medium supplemented with glucose. It was determined that enriched carrot juice may serve as a good nutrient source and could be used for citric acid production by K-168strain.

Probabilistic behavior of semi-active isolated buildings under pulse-like earthquakes

Seda Öncü-Davas,Cenk Alhan 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.3

Seismic isolation systems employ structural control that protect both buildings and vibration-sensitive contents from destructive effects of earthquakes. Structural control is divided into three main groups: passive, active, and semi-active. Among them, semi-active isolation systems, which can reduce floor displacements and accelerations concurrently, has gained importance in recent years since they don’t require large power or pose stability problems like active ones. However, their seismic performance may vary depending on the variations that may be observed in the mechanical properties of semi-active devices and/or seismic isolators. Uncertainties relating to isolators can arise from variations in geometry, boundary conditions, material behavior, or temperature, or aging whereas those relating to semi-active control devices can be due to thermal changes, inefficiencies in calibrations, manufacturing errors, etc. For a more realistic evaluation of the seismic behavior of semi-active isolated buildings, such uncertainties must be taken into account. Here, the probabilistic behavior of semi-active isolated buildings under historical pulse-like near-fault earthquakes is evaluated in terms of their performance in preserving structural integrity and protecting vibration-sensitive contents considering aforementioned uncertainties via Monte-Carlo simulations of 3-story and 9-story semi-active isolated benchmark buildings. The results are presented in the form of fragility curves and probability of failure profiles.

Enhanced cycling stability performance for supercapacitor application of NiCoAl-LDH nanofoam on modified graphite substrate

Seda Polat,Gülten Atun 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.99 No.-

The high capacitive, cost-effective, non-toxic nickel–cobalt layered double hydroxide nanofoampseudocapacitive electrode materials doped with aluminium (NiCoAl-LDH) have been hydrothermallysynthesized on the electrochemically modified graphite (G) substrate with zinc and copper (Zn/G and Cu/Zn/G). The Al3+ diffusion in the LDH during the hydrothermal synthesis resulted in an ultrathin nanofoammorphological structure well adapted to the entire surface of Zn/G and Cu/Zn/G. The high arealcapacitance of the best efficient NiCoAl-LDH/Cu/Zn/G electrode of 2.17 F cm 2 at 5 mA cm 2 decreases to1.83 mF cm 2 at 75 mA cm 2 showing an excellent rate capability of 84%. An asymmetric supercapacitor(ASC) designed with graphite as negative electrode exhibits an energy density of 29.3 Wh kg 1 at a powerdensity of 575 W kg 1. It still remains at 5.6 Wh kg 1 at a higher power density of 3477 W kg 1 at adischarge time of 5.8 s indicating ultra-fast energy storage ability of the G//NiCoAl-LDH/Cu/Zn/G device. Its cyclic tests were also made by constructing a coin-cell-type device for industrial applications. Thecapacitance of the coin-cell operating within 1.6 V was protected around 100% even after over 10,000charge–discharge cycles at the current densities up to 1.8 mA cm 2.

Probiotic Characteristics of Lactobacillus brevis KT38-3 Isolated from an Artisanal Tulum Cheese

Seda Hacıoglu,Buket Kunduhoglu 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.6

Probiotics are living microorganisms that, when administered in adequate amounts, provide a health benefit to the host and are considered safe. Most probiotic strains that are beneficial to human health are included in the “Lactic acid bacteria” (LAB) group. The positive effects of probiotic bacteria on the host’s health are speciesspecific and even strain-specific. Therefore, evaluating the probiotic potential of both wild and novel strains is essential. In this study, the probiotic characteristics of Lactobacillus brevis KT38-3 were determined. The strain identification was achieved by 16S rRNA sequencing. API-ZYM test kits were used to determine the enzymatic capacity of the strain. L. brevis KT38-3 was able to survive in conditions with a broad pH range (pH 2-7), range of bile salts (0.3%-1%) and conditions that simulated gastric juice and intestinal juice. The percentage of autoaggregation (59.4%), coaggregation with E. coli O157:H7 (37.4%) and hydrophobicity were determined to be 51.1%, 47.4%, and 52.7%, respectively. L. brevis KT38-3 produced β-galactosidase enzymes and was able ferment lactose. In addition, this strain was capable of producing antimicrobial peptides against the bacteria tested, including methicillin and/or vancomycin-resistant bacteria. The cellfree supernatants of the strain had high antioxidant activities (DPPH: 54.9% and ABTS: 48.7%). Therefore, considering these many essential in vitro probiotic properties, L. brevis KT38-3 has the potential to be used as a probiotic supplement. Supporting these findings with in vivo experiments to evaluate the potential health benefits will be the subject of our future work.

Evaluation of shear bond strength of repair acrylic resin to Co-Cr alloy

Seda Cengiz,Duygu Sarac,Seniha Baba,Tolga Kulunk,safak Kulunk 대한치과보철학회 2014 The Journal of Advanced Prosthodontics Vol.6 No.4

PURPOSE The purpose of this study was to investigate the impact of different surface treatment methods and thermal ageing on the bond strength of autopolymerizing acrylic resin to Co-Cr. MATERIALS AND METHODS Co-Cr alloy specimens were divided into five groups according to the surface conditioning methods. C: No treatment; SP: flamed with the Silano-Pen device; K: airborne particle abrasion with Al2O3; Co: airborne particle abrasion with silica-coated Al2O3; KSP: flamed with the Silano-Pen device after the group K experimental protocol. Then, autopolymerized acrylic resin was applied to the treated specimen surfaces. All the groups were divided into two subgroups with the thermal cycle and water storage to determine the durability of the bond. The bond strength test was applied in an universal test machine and treated Co-Cr alloys were analyzed by scanning electron microscope (SEM). Two-way analysis of variance (ANOVA) was used to determine the significant differences among surface treatments and thermocycling. Their interactons were followed by a multiple comparison' test performed uing a post hoc Tukey HSD test (α=.05). RESULTS Surface treatments significantly increased repair strengths of repair resin to Co-Cr alloy. The repair strengths of Group K, and Co significantly decreased after 6,000 cycles (P<.001). CONCLUSION Thermocycling lead to a significant decrease in shear bond strength for air abrasion with silica-coated aluminum oxide particles. On the contrary, flaming with Silano-Pen did not cause a significant reduction in adhesion after thermocycling.

Description of Pediatric Tuberculosis Evaluated in a Referral Center in Istanbul Turkey

Seda Geylani Gulec,Leyla Telhan,Tanyel Koçkaya,Ela Erdem,Banu Bayraktar,Ayse Palanduz 연세대학교의과대학 2012 Yonsei medical journal Vol.53 No.6

Purpose: Diagnosis of tuberculosis (TB) in children is more challenging than in adults. This study aimed to describe demographical, clinical and laboratory findings of children diagnosed with tuberculosis in Turkey, including the issues of contact tracing, culture positivity and forms of the disease. Materials and Methods: Clinical and laboratory data of 51 children with a mean age of 8.0±4.6 years who were diagnosed with TB were retrospectively reviewed. Main diagnostic tools included tuberculin skin test, chest X-ray, sputum/gastric aspirate culture with sensitivity testing,and direct microscopy for acid-fast bacilli on available samples. Clinical characteristics and outcomes of the patients were examined. Results: Thirty-six (70.6%) children were diagnosed with intra-thoracic and 15 (29.4%) with extra-thoracic tuberculosis. Twenty-eight of the patients had a positive Bacillus Calmette-Guérin vaccine scar (28/51, 54.9%) and 23/51 (45.1%) had a positive tuberculin skin test. An adult TB contact was identified in 27 (52.9%) of the cases. On direct microscopy, acid-fast bacilli were found in nine (17.6%) patients and positive culture for Mycobacterium tuberculosis was found in 19 (37.3%). Drug resistance to isoniazid was detected in four (7.8%). One patient with nephrotic syndrome and miliary tuberculosis died during follow-up. All other patients responded well to the treatment. Conclusion: Focusing on active contact tracing among all household contacts of tuberculous cases may be helpful in early identification and controlling childhood disease, even in regions with low disease prevalence. Adopting a suspicious and proactive approach in this particular age group is warranted.

Human Papillomavirus Genotype Distribution and E6/E7 Oncogene Expression in Turkish Women with Cervical Cytological Findings

Tezcan, Seda,Ozgur, Didem,Ulger, Mahmut,Aslan, Gonul,Gurses, Iclal,Serin, Mehmet Sami,Giray, Burcu Gurer,Dilek, Saffet,Emekdas, Gurol Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

Background: Infection with certain human papillomavirus (HPV) genotypes is the most important risk factor related with cervical cancer. The objective of the present study was to investigate the prevalence of HPV infection, the distribution of HPV genotypes and HPV E6/E7 oncogene mRNA expression in Turkish women with different cervical cytological findings in Mersin province, Southern Turkey. Materials and Methods: A total of 476 cytological samples belonging to women with normal and abnormal cervical Pap smears were enrolled in the study. For the detection and genotyping assay, a PCR/direct cycle sequencing approach was used. E6/E7 mRNA expression of HPV-16, 18, 31, 33, and 45 was determined by type-specific real-time NASBA assay (NucliSENS EasyQ$^{(R)}$HPV v1.1). Results: Of the 476 samples, 106 (22.3%) were found to be positive for HPV DNA by PCR. The presence of HPV was significantly more common (p<0.001) in HSIL (6/8, 75%) when compared with LSIL (6/14, 42.9%), ASC-US (22/74, 29.7%) and normal cytology (72/380, 18.9%). The most prevalent genotypes were, in descending order of frequency, HPV genotype 66 (22.6%), 16 (20.8%), 6 (14.2%), 31 (11.3%), 53 (5.7%), and 83 (4.7%). HPV E6/E7 oncogene mRNA positivity (12/476, 2.5%) was lower than DNA positivity (38/476, 7.9%). Conclusions: Our data present a wide distribution of HPV genotypes in the analyzed population. HPV genotypes 66, 16, 6, 31, 53 and 83 were the predominant types and most of them were potential carcinogenic types. Because of the differences between HPV E6/E7 mRNA and DNA positivity, further studies are required to test the role of mRNA testing in the triage of women with abnormal cervical cytology or follow up of HPV DNA positive and cytology negative. These epidemiological data will be important to determine the future impact of vaccination on HPV infected women in our region.

Investigation of Ocular Neovascularization–Related Genes and Oxidative Stress in Diabetic Rat Eye Tissues After Resveratrol Treatment

Atiye Seda Yar,Sevda Menevse,Irem Dogan,Ebru Alp,Volkan Ergin,Ahmet Cumaoglu,Aysel Aricioglu,Abdullah Ekmekci,Adnan Menevse 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.4

Changes in vascular endothelial growth factor (VEGF), angiotensin-converting enzyme (ACE), matrix metalloproteinase (MMP)-9, and endothelial nitric oxide synthase (eNOS) mRNA expression profiles and oxidative stress in the eye tissue microenviroment may have important roles in ocular neovascularization and permeability in proliferative diabetic retinopathy. The present study investigated the effects of resveratrol (RSV) treatment on the mRNA expression profile of VEGF, ACE, MMP-9, and eNOS, which are associated with vascular neovascularization, and glutathione, protein carbonyl,and nitrite–nitrate levels, which are markers of oxidative stress in eyes of diabetic rats. Twenty-four Wistar albino male rats were divided into four groups. After diabetes induction with streptozotocin (10 mg/kg/day) RSV was administered to the RSV and diabetes mellitus (DM) + RSV groups for 4 weeks. The mRNA levels were measured by quantitative real-time polymerase chain reaction assay, and biochemical estimations were determined with spectrophotometric assays in eye homogenates. The mRNA expression levels of VEGF, ACE, and MMP-9 were increased in the DM group compared with the control group, and RSV treatment decreased their mRNA levels. Expression of eNOS mRNA was increased in the RSV and DM groups and decreased in the DM+ RSV group. Nitrite–nitrate levels and protein carbonyl content were increased and glutathione levels were decreased in the DM group compared with controls. Consequently, these data suggest that RSV suppressed the expression of eNOS, which is actively involved in the inflammation and healing process in chronic diabetes. Although oxidative stress was increased in eye tissue from diabetic rats, mRNA levels of VEGF, MMP-9, and ACE genes associated with vascular remodeling did not change in diabetic eyes.