Effects of Interleukin-1β, Tumor Necrosis Factor-α and Interferon-γ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

Kwon, Young-Man,Kim, Se-Won,Ko, Seon-Yle The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of 30μM SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration(3μM) of SNP enhanced it. Althogh the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone matabolism and bone pathophysiology.

Carotenoids 식이와 알코올이 혈액학적 성분과 간조직에 미치는 영향에 관한 연구

조만희,김연선,이상한,우기민,장예진,김창세 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.2

Alcohol is well known agent which can damage the human tissues such as liver via stimulating lipid peroxidation and storage, denaturation of macromolecules, and inhibiting protein metabolism. On the other hand, carotenoids in addition to vitamins A, C, E and lipoic acid, play important roles in protecting these oxidative damages as well as preventing the production of free radicals. This study was carried out to elucidate the precise effects of alcohol administration into rats on the antioxidative functions of dietary carotenoids and isolated β-carotene, and to find out any parameters to uncover more detailed biochemical mechanisms of these agents. For these purposes, two different approaches were performed: 1) examination of the changes in hematological parameters (e.g., total proteins, A/G ratio, ALT/AST et. al) and statistical correlations among inter- and intragroups, 2) examination of the histopathological changes by an electron microscope. The results were analyzed and summarized as following; (1) Carrot diet for one week caused a slight increase in albumin. A/G ratio and AST levels, and a slight decrease in ALT, ALP, BUN and uric acid levels. Two-week alcohol administration following carrot diet increased total proteins, albumin A/G ratio and BUN levels. However, both groups were shown to have little significant changes in cholesterol concentrations. (2) Cheese diet for one week caused a significant increase in total proteins, albumin, AST, ALP, uric acid and total cholesterol levels, and a significant decrease in A/G ratio and glucose concentrations. However, two-week β-carotene diet following cheese intake increased total proteins albumin, A/G ratio, BUN and total cholesterol levels, whereas AST, ALT, ALP and uric acid levels were decreased. (3) Alcohol administration for one week caused an increase in AST activities and a decrease in total proteins, albumin, ALT, ALP and BUN levels. A successive β-carotene diet following alcohol administration increased total proteins, albumin, ALT, ALP and BUN levels, whereas decreased AST and uric acid levels. However, there were not significant changes in A/G ratio, glucose and total cholesterol levels in both groups. (4) β-carotene diet for one week caused a slight increase in albumin, glucose, AST and uric acid levels, and a slight decrease in ALP and BUN levels. Two-weeks alcohol administration following β-carotene diet increased albumin, glucose, BUN and total cholesterol levels, whereas decreased AST, ALT, ALP and uric acid levels. (5) Although the pathological investigation on the liver did not reveal significant changes, cheese diet group (CH-BC/1-CH) was shown to have some lipid deposits. Some results were unexpected and different from typical hematological changes shown by other researchers. Nonetheless, these results strongly suggest that the liver damage or hepatism caused by alcohol intake affects many kinds of biochemical metabolisms, which results in significant changes in many hematological parameters. In addition, dietary carotenoid and isolated β-carotene were shown to have protective roles against the biochemical changes by alcohol intake.

급성기 뇌졸중 환자 자살 사고

김세주,김영신,최낙경,서동향,이병철,이만홍 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.2

연구목적: 일반적으로 자살과 자살 사고의 가장 주된 위험 인자는 우울증, 불안장애와 같은 정신과적 질환의 동반 유무이다. 그러나 정신과적 질환 외에 신체적 질환의 동반 유무 또한 자살 및 자살 사고에 영향을 미치는 것으로 알려져 있다. 일반적인 신체 질환과 마찬가지로 뇌졸증 환자들에 있어 자살율이 증가한다는 몇몇의 보고들이 있다. 그러나 우리나라에서는 뇌졸중 환자의 자살 사고에 대한 연구가 보고된 바가 없다. 따라서 본 연구에서는 급성기 뇌졸중 환자들을 대상으로, 자살 사고의 발현율을 조사하고, 자살 사고 유무에 따른 사회문화적 변인 및 신경과적, 정신과적 임상 양상에 있어서의 차이에 대해 알아보고자 하였다. 방법: 1999년 7월부터 2000년 6월까지 한림대학교 성심병원 뇌졸중 센터에 입원한 77명의 급성기 뇌졸중 환자를 대상으로 하였다. Beck Suicidal Ideation Scale(BSIS)을 이용하여 자살 사고를 평가하였고, 우울 증상과 불안 증상은 각각 Beck Depression Inventory(BDI)와 Beck Anxiety Inventory(BAI)를 사용하였다. 사회적 지지체계의 정도는 사회적 지지체계 척도(Social Support Scale)를 사용하였으며, 뇌졸중 후 신경학적 장애의 정도는 National Institutes of Health Stroke Scale(NIHSS)와 Barthel's Index를 사용하였다. 결과: 약 35%의 뇌졸중 환자들이 심한 자살 사고를 보고하였다. 심한 자살 사고군에서 심하지 않은 자살 사고군에 비해 BDI, BAI 그리고 사회적 지지체계 척도 점수가 높은 반면, Barthel's index와 NIHSS는 두 군간에 유의한 차이를 보이지 않았다. 심한 자살 사고를 지닌 뇌졸중 환자의 대부분은 우울 증상이나 불안 증상을 가지고 있었으나, 일부의 환자들은 우울 증상이나 불안 증상 없이 자살 사고를 보고하였다. 결론: 급성기 뇌졸중 환자들의 많은 수가 정신과적 평가와 개입이 필요한 자살 사고를 가지고 있었다. 따라서 모든 급성기 뇌졸중 환자들을 대상으로 정기적으로 불안 및 우울 증상에 대한 평가와 더불어 직접 또는 간접적인 방법을 통한 자살 사고의 평가가 반드시 필요할 것으로 생각된다. Objective: The presence of psychiatric disorders including depression and anxiety disorders is considered to be the most important risk factor of suicide. Also, suicidal risk is known to be increased in patients who have serious medical illnesses. Like in patients with other medicalillness, some authors reported that suicidal risk is increased in patients with stroke. But there have been no reports with korean patients. The aim of this study is to investigate the frequency of suicidal ideation of patients in the acute stage of stroke, and to examine the demographic characteristics, and psychiatric and neurological symptoms between the patients with and without suicidal ideation. Method: Seventy seven hospitalized stroke patients at the Hallym Stroke Center from July of 1999 to June of 2000 were included in this study. Beck Suicidal Ideation Scale(BSIS) was used to evaluate suicidal ideation. Beck Depression Inventory(BDI) and Beck Anxiety Inventory(BAI) for depression and aniety, and Social Support Scale(SSS) for social support system of the stroke patients were used. Neurologic disabilities were rated with National Institutes of Health Stroke Scale(NIHSS) and Barthel's Index. Results: Thirty five percent(N=27) of the stroke patients reported severe suicidal ideation. Scores of BDI, BAI and SSS were higher in the patients with severe suicidal ideation than their counterpart. There were no differences in Barthel's index score and NIHSS between two groups. Most patients with severe suicidal ideation had depressive or anxiety symptoms. But patients without depressive or anxiety symptoms also reported severe suicidal ideation. Conclusions: Thirty five percent of stroke patients in acute stage of their illness have severe suicidal ideation that requires careful psychiatric evaluation and intervention. Inclusion of routine assessment of suicidal ideation in these patients is recommended.

HPLC를 이용한 Benfuracarb의 측정법에 관한 연구

조만희,김창세,이상한,조경환 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.2

The measurements of pesticide Benfuracarb were studied and following conditions were proposed as a most good methods; 1. There were detected at 274 nm by means of Hitachi 200-20 spectrophotometer. 2. Pesticide Benfuracarb mesurement was performed by means of ISCO model 2350, spherisorb ODS column, 80% acetonitrile as a solvent phase, flow rate 0.5 ml/min, chart speed 0.5 cm/min, and 25℃ oven temperature. 3. The relationships between the concentration and absorbance of pesticide Benfuracarb were linear from 5 to 50 ppm. 4. Deproteinzation and the recovery rate would be used satisfactorily in Folin-Wu.

重回歸分析에 의한 勞動者 雇傭豫測에 관한 硏究 : 5人以上 事業體 勤勞者 中心으로 Based on the Employees in the Working Places which have 5 or more full-time Workers

吳世萬,李秉源 동국대학교 경영대학원 1991 經營論叢-東國大學校 經營大學院 Vol.15 No.-

The purpose of this study is to forcast the employment of the employees in the working places which have 5 or more than full-time workers. The main conclusions of this study are summarized as follows: First, the employment is decided on the three factors which are POP(population), GNP/CPI(Gross National Product divide by Consumer's Price Index), and GNP/INC(Gross National Product divide by Income). Also, the flexibilities for each factor are 0.3, 3.7 & 4.6, and GNP is considered the most flexible factor for the employment. Second, it is estimated that 6,268 thousand persons will be employed in 1995, and it is expected that the employment will grow to 2.9% yearly during 6 year 1990∼1995, while the employment has grown up by 7.1% yearly during the last 14 years between 1976∼1989.

C++클래스 프로그래밍 지원 도구

오세만,연주흠 東國大學校 1992 東國論叢 Vol.31 No.-

The C++ language is object-oriented programming language which is derived from C language. Although C++ language is based on conventional programming language, it has few drawbacks in use. Particularly, the class programming which enables the reuse of the program is very hard without a supporting tool. When we program the software package using C++ ckasses, we need an information about the relating classes. To accomplish this, we have implemented a system which aids the C++ class programming. The main works of this system are that information about classes in acquired automatically and programmed classes can be added to the class library with very little effort. The system extracts information form classes by parsing the C++ source code and manages class library based on class inheritance hierarchy. And then this information about classes and the class source code are provided when the class library is browsed. So we can do the C++ class programming easily by using the system.

이미지 데이터베이스를 위한 정보 검색 시스템의 설계 및 구현

김만순,오세봉,김미연,민은미,박미영,정문숙,문현수,김상욱 강원대학교 정보통신연구소 2002 정보통신논문지 Vol.6 No.-

This paper presents design and implementation of an information retrieval system for a large volue of an image database. Our system supports three kinds of queries: attribute-based queries, keyword-based queries, and color-based queries. The attribute-based query is the most-typical query that finds target images based on their attribute values. The keyword-based query is to find target images by referring to their description information their attribute values. Finally, the color-based query is to search for target images by comparing the colors of a query image with those of images in the database. In this paper, we presents the system architecture and approaches adopted in our system for supporting the above three queries effectively and efficiently. We also discuss the user interface of our system that enables users to manipulate our system easily and conveniently.

트리 패턴 매칭 최적화

오세만,김정숙 東國大學校 1995 東國論叢 Vol.34 No.-

It is necessary to optimize target machine independent intermediate code since recent researches on complier development are interested retargetable optimization compiler that can produce the code which can be applied for various target machine. there are various kinds of intermediate languages which are designed for the structures of each compiler. We designed and implemented the intermediate code optimizer that performs the optimization with tree pattern matching techniques by selecting EM intermediate language that is abstract machine code and was used an amsterdam Compiler Kit(ACK). This optimizer system consists of two parts : the tree pattern generator and the tree pattern matcher. The tree pattern generator accepts a table which has 645 optimization patterns and was presented by ACK system. And it generates the tree pattern which is suitable for the tree pattern matcher. The tree pattern matcher produces optimized tree in accordance to constructed tree pattern and performs the actual tree pattern optimization actions. As a result, compare to the most of the traditional string pattern matching methods, it gives about 25% of the code reduction effect for the source code - EM code - and it can reduce re-scan numbers for the basic block. We also found that the optimized code affected to program execution time, and contributed to improve the execution time by about 36%.

AST의 자동적인 구성

오세만,박진기 東國大學校 1990 論文集 Vol.29 No.-

The task of constructing a complier for a particular source language is complex and large. The complexity of compilation process can be reduced if a compiler is designed modularly. So an intermediate from is needed to interface the phases of the compiler. One type of intermediate form is a tree-structured code which is a suitable form for optimization compilers. An AST is a tree-structured intermediate code which does contain the information necessary to next phase of syntax analysis. Most compliers use AST as an intermediate form because of the efficient representation of the source program. The design and obtaining of AST is important in the compiler design process. In this paper, we have implemented the automatic AST generation system. For this work, we designed GDL which is similar to grammar definition in formal language theory and able to describe the structures of AST. And then we implemented the GDLTS that generates the parser constructing the AST automatically according to the specification of GDL. This system can be used as a tool for constructing AST of source program being complied and also used as AST designing tool.

S-아데노실-L-메치오닌 의존성 메칠화 반응의 억제인자에 관한 연구

조만희,김창세,이상한 순천향대학교 1992 논문집 Vol.15 No.4

Protein carboxyl methyltransferase is an enzyme whose function in eucaryotic cells remains controversial. Early studies suggested that protein carboxymethylation subserved a regulatory, post-translational role in such diverse processes as purification and characterization of protein enzyme. In this paper, we present the purification and characterization of the activties of protein methylase and SAM(S-Adensoyl-L-Methionine)synthetase were examined inhibitor protein methylase Ⅱ by Bio-Gel p-6 column(1.8cm i.d×112cm) and μBondapak C18 column (3.9mmi.d×30cm) High-performance liquid chromatography. The column was eluted with linear gradient of acetonitrile(0-10% in water) in 30 min run with 0.33% gradient slope/min at a flow rate of 1.0ml/min, and the elution was monitored at 214nm.