Collagenase와 esterase가 상아질 접착강도와 nanoleakage에 미치는 영향

정영정,한세현,김종철,이상훈,김정욱,김영재,장기택 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.3

본 연구는 상아질 접착계면에서 collagenase와 esterase가 접착강도와 극미세누출에 미치는 영향을 살펴보기 위해 시행하였다. 발치된 치아의 교합면 상아질에 Single Bond 2(SB)와 Clearfil SE bond(SE)를 사용하여 상아질-레진 접착시편을 제작하고,시편을 인산완충용액(PBS)에 24시간(I),또는 PBS(II),collagenase 용액(III),esterase 용액(IV)에 4주간 보관 한 후 질산은 용액으로 염색하였다. 시편의 미세인장접착강도(μTBS)와 질산은 침투 면적을 측정하여 다음과 같은 결과를 얻었다. 1. SB군의 접착강도가 II∼IV군에서 SE군에 비해 낮은 값을 나타내었다(p<O.05). SB군은 II∼IV군의 접착강도가 I군에 비해 낮은 값을 보였으나(p<O.05),SE군의 접착강도는 I∼IV군간에 차이를 보이지 않았다(p>O.05). 2. SB군의 질산은 침투 면적이 SE군에 비해 높았으며(p<O.05),SB군과 SE군에서 질산은 침투 면적은 I ∼ IV군간에 차이를 보이지 않았다(p>O.05). 3. 접착강도와 질산은 침투 면적은 SE군의 I, II,III군에서 음의 상관관계를 보였다(p<O.05). The purpose of this study was to evaluate the effects of collagenase and esterase on dentin bond strength and nanoleakage. Resin composites were bonded to occlusal dentin of premolars with Single Bond 2(SB) and Clearfil SE Bond(SE). After the microtensile specimens were prepared and stored in PBS for 24 hours(I) or, PBS(II), collagenase(III), esterase(IV) solution for 4 weeks, the specimens were stained with silver nitrate solution. Microtensile bond strength(μTBS) and silver penetration area were measured and, the results were as follows: 1. For group II, III, and IV, the bond strengths of SB were lower than those of SB(p<0.05). The bond strengths of SB II, III, and IV were lower than that of SB I(p<0.05). There was no difference among the bond strengths of SE I ∼ IV groups(p>0.05). 2. Silver penetration areas of SB were higher than those of SE for all storage groups(p<0.05). In SB and SE groups, there was no significant difference of silver penetration area among I ∼ IV groups(p>0.05). 3. SE I, II, and III showed inverse relationship between the bond strengths and the silver penetration areas(p<0.05).

Cu(In,Ga)Se₂ 나노입자을 이용한 광흡수층 치밀화에 따른 Se 분위기의 열처리 효과

윤경훈(Yoon, Kyung-Hoon),김기현(Kim, Ki-Hyun),안세진(Ahn, Se-Jin),안병태(Ahn, Byung-Tae) 한국신재생에너지학회 2006 한국신재생에너지학회 학술대회논문집 Vol.2006 No.06

나노입자를 이용하여 치밀한 Cu(In,Ga)Se₂ 태양전지용 광흡수층을 제조하기 위해 먼저, 콜로이달 방법으로 합성된 20nm이하의 CIGS 나노입자를 저가의 스프레이 법을 이용하여 CIGS 막을 제조하였다. 제조된 CIGS막을 two-zone RTP (rapid temperature Process) 방법으로 Se 분위기 안에서 열처리를 행하였다. 입자의 치밀화를 위해 기판의 온도, Se 증발온도와 수송가스의 유량을 조절하여 CIGS 입자성장을 행하였다. 그러나, Se의 증발온도가 높을수록 CIGS와 MO 박막 사이에서 MoSe₂ 층이 형성되었다. 형성된 MoSe₂층의 부피 팽창으로 인해 하부의 유리기판과 Mo층 사이에서 peeling off 현상이 발생했다. 이러한 Peeling off현상을 억제하면서 CIGS 나노입자 성장을 하기 위해, Se 공급을 빨리 할 수 있도록 Se의 증기압을 높였으며, 최적조건에서 급속 열처리 공정을 통해 CIGS 나노입자 성장과 치밀화를 위한 소결거동을 관찰하였다.

담낭암의 발생에서 담췌관합류이상의 역할 규명과 암화과정에 관한 연구

한기준,송시영,정재복,이세준,김호근,강진경 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.4

논문(論文) : 아라가야(阿羅伽耶)의 성립기반과 영역의 변천

김세기 ( Se Ki Kim ) 대구사학회 2012 대구사학 Vol.106 No.-

The Formation and Territory of Aragaya deducing from Archaeological Sources Kim, Se-ki [Abstract] Aragaya, existed in Haman, was the state which formed and developed from Anya, a small country belong to the Three Han States, in the third century. There have been negative views about the existence of Anya, because a wooden grave proving the formation of small countries didn`t excavate at Haman. But recently, a large scale of dolmens on the boundaries of graves were excavated at Jindongri. When we deduce from the distinctions of the scale and facilities contained therein, we can conclude that a high level of political authority was developed at Haman. According to archaeological materials, the people established the foundation of early state at Haman were the group constructed dolmens at Jondongri. The early state at Haman was formed at Jindongri, unified a Haman basin and the group built dolmens around it, and transferred the center to the Dohangri area. That has the root of Anya, and the territories were included the central zone of Haman basin, the coastal area of Jindongri, and the district of Gunbuk around Nam River. Anya was developed to Anra on the strength of the foreign trade by utilizing the southern coast and the advantage of waterways along Nam River and Nakdong River in the late of third and the early of fourth centuries. And the state expanded to the Chilwon area in this century. The main form of grave was changed to pit tomb in the fifth century. The ancient graves of Dohangri, the complex of Aragaya graves, had some characteristics, such as the very fine plane form flattened to more than 5.5:1 ratio drapery, the structure of a single room, and the burial of the living with the dead inside the central room. Therefore, Aragaya was developed from the period of using pit tomb as a main form of grave.

질소 및 셀레늄 분위기 열처리가 나노 입자 Cu(In, Ga) Se₂ 광흡수층의 치밀화에 미치는 영향

김기현(Kim, Ki-Hyun),안세진(Ahn, Se-Jin),전영갑(Chun, Young-Gab),박병옥(Park, Byun-Ok),윤경훈(Yoon, Kyung-Hoon) 한국신재생에너지학회 2005 한국신재생에너지학회 학술대회논문집 Vol.2005 No.06

나노 입자 분무 기법을 이용한 Cu(In,;Ga)Se₂ (CIGS) 광흡수층 제조 기법은 고진공 장치를 사용하지 않는다는 점에서 대면적 저가형 CIGS 태양전지 양산에 적합한 차세대 기술로 인식되고 있다. 그러나 일반적으로 스프레이 된 상태의 CIGS충 자체는 태양전지 제조에 적합하지 않은데 이는 스프레이 막의 다공성 구조 때문이다. 본 연구에서는 나노입자 분무 기법을 이용하여 증착한 CIGS 광흡수층막을 질소 또는 셀레늄 분위기에서 열처리함으로써 태양전지 제조에 적합한 치밀한 구조의 CIGS 광흡수충을 제조하고자 하였다. 실험 결과, 질소 분위기 500?C의 온도에서 1시간 열처리하여도 CIGS 나노 입자의 성장은 거의 일어나지 않는 것으로 나타났다. 반면 셀레늄 분위기 500?C의 온도에서 30분 열처리시 입자 크기가 1{mu}m이상인 치밀한 광흡수층을 얻을 수 있었다. 본 결과는 CIGS 나노 입자의 입자 성장 반응에서 열에너지 단독에 의한 표면 에너지 감소 효과는 미미하며 셀레늄 증기의 역할이 더욱 크다는 것을 의미하는 것이다.

비진공 나노입자 코팅법을 이용한 CIGS 박막 태양전지 제조

안세진(Ahn, Se-Jin),김기현(Kim, Ki-Hyun),윤경훈(Yoon, Kyung-Hoon) 한국신재생에너지학회 2006 한국신재생에너지학회 학술대회논문집 Vol.2006 No.06

A non-vacuum process for Cu(In,Ga)Se₂ (CIGS) thin film solar cells from nanoparticle precursors was described in this work CIGS nanoparticle precursors was prepared by a low temperature colloidal route by reacting the starting materials (CuI,;InI₃,;GaI₃;and;Na₂Se) in organic solvents, by which fine CIGS nanoparticles of about 20nm in diameter were obtained. The nanoparticle precursors were mixed with organic binder material for the rheology of the mixture to be adjusted for the doctor blade method. After depositing the mixture of CIGS with binder on Mo/glass substrate, the samples were preheated on the hot plate in air to evaporate remaining solvents ud to burn the organic binder material. Subsequently, the resultant (porous) CIGS/Mo/glass simple was selenized in a two-zone Rapid Thermal Process (RTP) furnace in order to get a solar ceil applicable dense CIGS absorber layer. Complete solar cell structure was obtained by depositing. The other layers including CdS buffer layer, ZnO window layer and Al electrodes by conventional methods. The resultant solar cell showed a conversion efficiency of 0.5%.

Ga 함량에 따른 Cu(In<SUB>x</SUB>Ga<SUB>1-x</SUB>)Se₂ 태양전지의 특성분석

김석기(Seok Ki Kim),권세한(Se Han Kwon),이두열(Doo Yeol Lee),이정철(Jeong Churl Lee),강기환(Ki Whan Kang),윤경훈(Kyung Hoon Yoon),안병태(Byung Tae Ahn),송진수(Jinsoo Song) 대한전기학회 1998 대한전기학회 학술대회 논문집 Vol.1998 No.7

담낭암 발생에서 담췌관합류이상의 역할 규명과 암화과정에 관한 연구

강진경,한기준,이세준,송시영,정재복,김호근 대한소화기학회 1998 대한소화기학회지 Vol.32 No.4

Baekground/Aims: To clarify the careinogenesis of gallbladder cancer (GBC) in patients with anomalous pancreaticobiliary ductal union (APBDU), we investigated the expression of mutated p53 protein and c-erbB2, the cell proliferation indices of Ki-67 and the histopathologic changes in gallbladder carcinoma and noncaneeraus mucosa which are associated with APBDU. Methods: In the clinical review, we analysed 62 patients (27 patients of GBC with APBDU and 35 patients of GBC without APBDU) who were diagnosed in Sevrance Hospital from January, 1992 to December, 1994. Immunohistochemical staining and histologic examination were performed on the specimens obtained from surgically resected gallbladders from the patients. For comparative study, the specimens were grogped as follows: 5 cases of carcinoma with APBDU, 4 eases of noneancerous mucosa with APBDU associated with GBC, 11 cases of noncancerous mucosa with APBDU not associated with GBC, 19 cases of carcinoma without APBDU and 7 cases of chronic inflammatory mucosa of gallbladder stones as controls. Results, GBC developed in 33.3% of patients with APBDU (especially, 66.7% of PC type) and showed no gallbladder stones. The frequency of papillary carcinoma was higher and depth of invasion was less in GBC with APBDU than in GBC without APBDU. Among 15 cases of noncancerous mucosa with APBDU, isolated dysplasia and adenomyomatous hyperplasia were noted in 10 cases and 4 cases, respectively. In one case of APBDU with GBC, the expression of p53 protein was noted in the focal area of low grade dysplasia. Cell proliferation indices of Ki-67 were stepwise increased with the progression of histologic findings from inflammation, papiUary hyperplasia, metaplasia and adenomyomatous hyperplasia to dysplasia. Particularly, remarkable increase was observed in adenomyomatous hyperplasia and dysplasia. Conclusions: The high incidence of isolated dysplasia and adenomyoma accompanying with increased cell proliferative indices seems to be closely related to th carcinogenesis of GBC in patients with APBDU. Additionally, the mutation of p53 may contribute to early event of carcinogenesis in some patients with APBDU.

불면을 호소하는 뇌실질내출혈 환자 치험 1례

김경묵,김세원,서유나,정우상,문상관,진철,조기호,권승원,Kim, Gyeong-muk,Kim, Se-won,Seo, Yu-na,Jung, Woo-sang,Moon, Sang-Kwan,Jin, Chul,Cho, Ki-ho,Kwon, Seungwon 대한중풍순환신경학회 2019 대한중풍.순환신경학회지 Vol.20 No.1

A case of a 46-year-old Korean male with post-stroke insomnia that induced by intracranial hemorrhage was presented. He was treated with Transcutaneous electric acupoint stimulation (TEAS) at BL62 and KI6 once per a day for 17 days. We recorded the patient's sleep time and time of asleep, and used PSQI-K to assess severity of patient's insomnia symptom. After using TEAS, there was improvement in patient's insomnia severity and the time of sleep. From the result of this case, electrical stimulation at acupoint can may be effective in treating post-stroke insomnia.

Effects of High Cholesterol Diet on Newly Generated Cells in the Dentate Gyrus of C57BL/6N and C3H/HeN Mice

KIM, Il Yong,HWANG, In Koo,CHOI, Ji Won,YOO, Ki-Yeon,KIM, Yo Na,YI, Sun Shin,WON, Moo-Ho,LEE, In Se,YOON, Yeo Sung,SEONG, Je Kyung Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.6

<P>In this study, we observed and compared the effects of a high cholesterol diet (HCD) on cell proliferation and differentiation in the subgranular zone of the dentate gyrus of C57BL/6N (B6, susceptible strain) and C3H/HeN (C3H, resistant strain) mice. Ki67 (a marker for cell proliferation) positive cells) were significantly decreased in HCD-fed B6 mice compared to those in B6 (49.7%) and C3H mice fed a low cholesterol diet (LCD). In addition, doublecortin (DCX, a marker for cell differentiation or neuroblasts)-immunoreactive cells in HCD-fed B6 mice were significantly decreased compared to those in LCD-fed B6 and C3H mice. These results suggest that B6 strains are sensitive to HCD, which impairs cell proliferation and differentiation.</P>