상아질 접착에서 collagenase와 esterase가 미세인장결합강도에 미치는 영향

정영정,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.2

상아질-레진 접착강도에 대한 collagenase와 esterase의 영향을 살펴보기 위해, 소구치의 교합면 상아질에 Single Bond 2와 Clearfil SE Bond로 접착을 시행하고 미세 시편을 제작하여 PBS, collagenase 용액, esterase 용액에 4주간 보관한 후 미세인장결합강도를 측정, 비교하여 다음과 같은 결론을 얻었다. 1. 모든 보관 용액에서 Single Bond 2의 미세인장결합강도는 Clearfil SE Bond보다 유의하게 낮았다(p<0.05). 2. Single Bond 2의 미세인장결합강도는 collagenase군이 PBS군, esterase군보다 낮았다(p>0.05). 3. Clearfil SE Bond의 미세인장결합강도는 esterase군이 PBS군에 비해 낮았으나(p>0.05), collagenase군보다는 높았다(p>0.05). Collagenase군은 PBS군에 비해 유의하게 낮은 미세인장결합강도를 보였다(p<0.05). The purpose of this study was to evaluate the effect of collagenase and esterase on the microtensile bond strength (μTBS) in dentin bonding. After resin composites were bonded to occlusal dentin, μTBS specimens were formed and stored in PBS, collagenase, or esterase solution. After 4-week storage, μTBS was determined and, the results were as follows: 1. μTBS values of Single Bond 2 were lower than those of Clearfil SE Bond for all storage medium (p<0.05). 2. In single Bond 2 group, collagenase solution lowered bond strength more than PBS and esterase solution (p>0.05). 3. In Clearfil SE Bond group, esterase solution lowered bond strength more than PBS(p>0.05). Collagenase solution lowered bond strength more than esterase solution(p>0.05) and PBS(p<0.05).

뇌종양 환자의 제왕절개술을 위한 전신마취 : 증례 보고 A case report

이헌근,전세현,손주태,신일우,정영균 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.4

A 28-year-old primigravida at 33 weeks gestation was referred to our hospital because of aggravation of seizures which occurred intermittently for the past 2 years. A brain magnetic resonance imaging scan taken at 33 weeks gestation showed a large brain tumor (7 × 5 × 4 cm) in the left high frontal lobe. She had a cesarean section under general anesthesia at 38 weeks and 1 day gestation. General anesthesia was induced with thiopental 250 mg, succinyleholine 75 mg and lidocaine 60 mg after a vecuronium 0.8 mg intravenous injection. The hyperventilation and mannitol used to reduce increased intracranial pressure (ICP) in this patient may be harmful and detrimental to the fetus before delivery. Achieving both ICP control and fetal well being at the same time during a cesarean section can be challenging to anesthesiologists. We report the anesthetic management of a cesarean section in a patient with a large brain tumor. (Korean J Anesthesiol 2002; 43: 515~519)

한국인 강박장애와 Val-158-Met Catechol-O-Methyltransferase 유전자 다형성 간의 상관성 연구

천근아,김세주,김찬형,구민성,남윤영,박성혁,이홍식 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.5

목적 COMT(Catechol-O-Methyltransferase) 효소는 도파민성과 노어아드레날린성 체계의 조절에서 매우 중요한 작용을 하며 염색제 22q11에 위치해 있다. 일부의 강박장애 환자들은 도파민 차단제에 좋은 치료 반응을 보이며 이런 현상은 COMT가 강박장애의 병인론에 역할을 하고 있을 가능성을 말해준다. Val-158-Met COMT 유전자의 다형성은 효소의 감소를 유도하는 L(저활성) 대림형질, 활성의 증가를 유발하는 H(고활성) 대립형질로 알려져 있으며 L 대립형질 또는 LL 유전형이 강박장애와 연관이 있다는 보고가 있으나, 아직까지 강박장애와 COMT 다형성 사이의 연관성에 대한 연구결과들은 일관되지 않다. 따라서 본 연구에서는 한국인 강박장애 환자들을 대상으로 Val-158-Met COMT 유전자의 다형성과의 연관성을 알아보고자 하였다. 방법 2002년 3월부터 2004년도 2월까지 약 24개월동안 서울지역 일 대학병원과 경기지역 일 대학병원 강박장애 클리닉을 방문하여 강박장애로 진단받은 124명의 환자들을 연구대상으로 하였고 정상 대조군으로는 나이와 성별을 고려하여 자원자 170명이 선별되어 포함되었다. 강박장애군과 정상 대조군의 혈액으로부터 유전자 추출 후 COMT 유전자 형벌 (genotyping)을 시행하였다. 다형성을 H/H 와 H/L, L/L 유전형으로 나눈 뒤 강박장애와 정상대조군 사이의 유전자 빈도를 카이 검정법으로 비교하여 연관성을 관찰하었다. 또한 강박장애 환자들에게 시행한 YBOCS로부터 측정한 강박장애 심각도 및 Hamilton Depression Raing Scale (HDRS) 총점과 COMT 유전자 다형성 사이의 연관성을 비교하였다. 결과 본 연구 결과 한국인 강박장애와 COMT 다형성 간에는 유의한 상관성이 관찰되지 않았다. YBOCS 심각도와 COMT 유전자형과의 유의한 연관성이 관찰되지 않았다. 그러나, 강박장애 환자군들의 COMT 유전자형 세 군 사이의 우울증상 점수에서는 유의한 차이가 관찰되었다. 결론 한국인에서 강박장애와 COMT 유전자 다형성 사이에는 연관성이 없었다. 그러나, COMT의 H 대립형질과 강박장애 군에서 공존하는 우울증상 척도가 유의한 연관성을 보인 현상은 COMT 유전자의 다형성이 강박장애의 공존 우울증상에 영향을 미칠 수 있는 가능성을 시사한다고 생각된다. Objectives : The definite cause of obsessive-compulsive disorder (OCD) is still unknown. Evidences from familial, twin and segregation studies support the role of a genetic factor. There are also growing evidence indicating that OCD has specific neurochemical and neuroanatomical basis. Derived from the effectiveness of treatment with dopamine receptor blocker in certain part of OCD patients (eg. SSRI treatment-resistant OCD), several candidate genes related to dopamine dysregulation have been hypothesized to play an important role in the development of OCD. One of them is the Catechol-O-Methyltransferase (COMT) gene. The aim of this study was to investigate the association between COMT and OCD in Korean population. Methods : 124 OCD patients and 170 normal controls participated in this study. Genomic DNA was extracted from their blood. The genotypes and allele frequencies of the COMT polymorphism between OCD group and control group were compared. We investigated the association between severity of OCD assessed by Yale-Brown obsessive compulsive symptom scale (YBOCS) and Hamilton depression rating scale (HDRS) scores and COMT polymorphism. Results : In this case-control study, we could not find any association between COMT gene polymorphism and development of OCD. In OCD group, patients with H/H genotype had significantly higher scores for the HDRS than those with H/L or L/L genotype. Conclusion : In this study, there was no difference in genotype distributions of COMT between OCD and control groups. However, H/H genotype of COMT gene polymorphism might be related to depressive symptoms in OCD patients.

수도관의 생물막 형성에 미치는 잔류염소와 파이프 재질의 영향

박세근,박재우,성권식,최성찬,김영관 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

This laboratory study examined the impact of free chlorine residual and pipe material on the formation of biofilm in drinking water distribution pipe surfaces. Result of heterotrophic plate counts(HPC) of the biofilm in the tap water- supplied reactor averaged 2.17×105CFU/cm2 on PVC and 2.43×105CFU/cm2 on STS 316, respectively. HPCs on the surface exposed to the tap water containing 0.2mg/L of free chlorinne residual averaged 4.24×104CFU/cm2 on PVC and 6.54×104CFU/cm2 on STS 316, respectively Average of HPC/Total direct counts in the tap water-supplied reactor ranged from 1.08%(PVC) to 1.26%(STS 316) and from 0.38%(PVC) to 0.65%(STS 316) in the reactor supplemented with disinfectant, resfectively. No correlation was observed between disinfectant addition and biofilm density. With regard to the biofilm formation, little difference existed between PVC and STS 316. Yellow and red pigmented bacteria were the dominant expressions in bulk fluid, whereas non-pigmented bacteria were found dominant in the biofilm. Pink/red pigmented bacteria were found to be facultative anaerobic, while yellow pigmented bacteria and non-pigmented bacteria were found to be obligate aerobic.

고령토로부터 알루미나 추출에 관한 연구(Ⅲ):염산에 의한 추출

최세영,이용근,이희수,김형국 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.1

The preparation conditions of alumina powder extracted by hydrochloric acid from domestic San-Cheong kaolin have been investigated. In order to obtain best conditions to extract alumina by hydrochloric acid, experiments carried out with various acid concentrations, reaction temperatures, reaction times, and pH. The optimum conditions were 20 wt.-% for hydrochloric aicd concentration, 100℃ for reaction temperature, 7hrs for reaction time and pH8. The yield of alumina found to be 89.2wt.-% under these conditions. Two drying methods, direct and ethanol distillation drying method to control the precipitated agglomeration have deen compared. The ethanol distillation drying method revealed more narrow particle size distribution and less agglomerate than the direct drying method.

손실 최소화를 위한 피더 재구성

김세호,좌종근 濟州大學校工科大學産業技術硏究所 1996 尖端技術硏究所論文集 Vol.7 No.2

Feeder reconfiguration is performed by opening/closing two types of switches. tie and sectionalizing switches. A whole feeder. or part of a feeder. may be served from another feeder by reclosing a tie switch linking the two while an appropriate sectionalizing switch must be opened to maintain radial structures. In loss reduction. the problem is to identify tie and sectionalizing switches that should be closed and opened. respectively, to achieve a maximum loss reduction. In this paper. it is introduced to propose the reconfiguration plan for loss minimization by comparison of two feeders' loss reduction formulars.

21세기의 초등학교 무용교육방향에 관한 연구

김세정,이근모 釜山大學校 師範大學 1998 교사교육연구 Vol.35 No.-

This study is aimed at objectifying several procedures for desiable direction of basic dance instruction. The procedures are first, consideration of 〈Journal of Korean Elementary school Dance Education Society〉 to seek current goal and emphasized point of dance instruction. Second, revealing educational value different from that of established by studying realistic and axiom definition of dance. Third, seeking education requirement necessary for fostering as desirable social component necessary for future by arrangement of opinion of future scholar to measuring desirable direction of 21century. Finally, predicting the desirable direction of 21century by these considerations mentioned previously. The result is that the direction of future dance education to foster desirable human for 21century should not emphasize only the body, creation, activity, etc emphasized as current motion education and inspiration of union consciousness should be include folk dance able to inspire union consciousness.

지방교육자치제 실시에 대한 평가연구

안세근 건국대학교 중원인문연구소 1998 중원인문논총 Vol.17 No.-

Educational local autonomy system aims at enhancing the right to receive education for students, teachers and parents, by guaranteeing the educational autonomy and specialization and peculiarity of the local education according to the democratic process. However, the current educational local autonomy system focusing on the structural aspects excessively is far from the "educational autonomy," which draws the residents' participation and the "self-involved autonomy," which enables them to experience themselves. The main purpose of this research is the evaluation of teachers on the reality of the current educational autonomy. The results are as follows: First, 65% teachers up to 225 said that the current educational autonomy system is necessary but should be improved. Second, 154 teachers(45%) show Consequently, teachers on the educational spot have no idea about the system or have little interest. Third, after executing the educational autonomy system there are more affairs in school, and still interference and control of the upper institutions. And all the more instructions and requirements are increased. Fourth, executing the educational autonomy system cannot provide the enhancement of the status as teacher, and what makes worse, the system is rather inclined to make their status lowered. Therefore, the teachers are likely to think the system in a negative manner. Consequently, the teachers of the unit school recognize the need on the system, however, they regard it negatively so that they burden more affairs and more interference and control from the upper institutions. The educational autonomy system should put an important emphasis on the change of the unit school. the unit school is the ground where the educational activities are accomplished. Educational administration should play the role of support and encouragement for well-performed education. Thus, for executing the fundamental purpose of the educational autonomy system, the inteference and control should be diminished, and the service administration will be adopted to support and stimulate the unit school.

경영전략과 아웃소싱의 적합 관계와 기업성과에 관한 연구

유세준,최근영 한국중소기업학회 2001 中小企業硏究 Vol.23 No.4

본 연구의 목적은 경영전략과 아웃소싱의 적합성이 기업성과에 어떠한 영향을 미치는가를 살펴보는데 있으며 구체적인 내용은 다음과 같다. 첫째, 국내 기업을 대상으로 경영전략, 아웃소싱 유형을 분석, 도출하고, 둘째, 각 유형에 따른 조직의 경영전략, 아웃소싱의 상황을 조사하여, 경영전략과 아웃소싱간의 관련성을 파악하고자 하며, 셋째, 기업 성과지표로서, 조직 유효성을 파악하여, 전략과 아웃소싱의 적합성과 기업성과는 어떠한 관계가 있는가를 살펴보았다. 본 연구는 상장제조업( 2001년, 6월말 기준) 433개 업체를 모집단으로 하여 우편조사와 방문조사를 병행하여 2001년 8월 1개월간 실시하였다. 회수된 설문서는 141개 였으며 회수율은 32.5%였다. 연구결과를 요약하면 다음과 같다. 첫째, 차별화 경영전략이 원가우위 경영전략 유형에 비하여 조직유효성으로 측정한 기업성과가 높은 것으로 나타났다. 따라서 경영전략 유형에 따라 기업성과는 영향을 받고 있다는 사실이 실증적으로 입증되었다. 둘째, 아웃소싱유형에 따라 기업성과에는 차이가 없는 것으로 나타났다. 즉, 아웃소싱 유형에 따라서 기업성과는 영향을 받지 않고 있다는 사실이 실증적으로 입증되었다고 할 수 있다. 셋째, 경영전략과 아웃소싱유형의 적합 관계에 따라 기업성과를 분석한 결과 적합한 기업은 적합하지 않은 기업보다, 조직유효성이 높은 것으로 나타났고 통계적으로 유의적인 차이가 발견되었다. 이 같은 발견은 우리에게 시사하는 바가 크다. 결국 경영전략과 아웃소싱유형의 적합관계는 장기적으로 볼 때, 기업의 수익성에도 영향을 미치고, 조직유효성에도 긍정적인 영향을 미칠 것이란 점을 유추해 볼 때, 경영전략을 감안한 탄력적인 아웃소싱 유형의 설계는 매우 중요하다는 사실을 알 수 있다. The study in the integrated approach of combining the strategy, outsourcing, and the performance attempted to accomplish the purpose of the study as follows: First, the purpose of the study was to analyze and to induce the types of the strategy of the growing firm in Korea. Second, it was to find out the relationships among the types of strategy, outsourcing, and the performance, in the process of examining the strategic situation and the outsourcing of the organization according to each type of the performance. Third, it was to find out the relationships between the adaptability of the managerial strategy and the performance of the firm, and outsourcing and the performance of the firm, in the process of identifying the effectiveness of organization. The study tried not only to analyze the relationships between the types of the strategy and outsourcing and the performances of the firm but also to identify the integrated relationship among the type of the strategy and outsourcing. In order to accomplish the purposes of the study, the study tried to measure the variables by collecting the positive data of the research questionnaire against the manufacturing firms. The measurable data returned was 141 of the respondents of the research questionnaire. The findings of the study were summarized as follows; First, the study found out the differences to the characteristics of the types of the strategy according to the environment. The characteristics of the innovational types was shown high in terms of the effectiveness. Second, the study found out no differences in terms of the effectiveness according to the characteristics of the types of the outsourcing pattern. Third, the study found out that the adaptive firm than the non-adaptive firm was shown high in terms of the effectiveness. For the effectiveness the differences was shown statistically significant. In the conclusion, the findings of the study would give a contribution to accomplish the innovation of the outsourcing system of the firm in korea.

PelB Signal Sequence로 유도된 제조합 인간 상피세포 증식인자 분비 발현 벡터의 제조

박세철,남정현,김정근,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

전사개시 단계중에 삽입되는 N말단의 methionine이 제거되고 pelB signal sequence를 삽입하여 인체 epidermal growth factor (hEGF)가 직접 분비발현 되도록 고안된 T7과 tac promoter에 의하여 각각 발현되는 pYHB101과 pYHB2를 제조하였다. 또한 T7 promoter에 의하여 발현되지만 signal sequence를 삽입하지 않은 pYHB1도 제조하여 각각 제조된 재조합 plasmid들의 발현 및 분비효율을 비교하였다. pelB signal sequence hEGF 유전자를 갖도록 제조된 pYHB101을 포함하고 있는 균주가 pYHB1보다 균체 성장속도는 낮았지만 pYHB101을 1 mM IPTG로 5시간 induction 하였을 때 13 mg/l의 rhEGF가 분비발현되어 T7 promoter의 발현 체계를 갖는 경우가 tac promoter보다 높은 발현 효율을 보였다. 이때 pYHB101에서 발현되는 rhEGF의 발현량은 준최적 조건에서 14 mg/l이었다. We have designed nucleotide sequences of hEGF structural gene to eliminate the N-terminal methionine residue incorporated during the translation initiation step, and constructed recombinant human epidermal growth factor (rhEGF) secretion plasmids pYHB101, and pYHB2 in which pelB signal sequence-hEGF gene was expressed under the control of the T7, and tac promoter, respectively. We also constructed pYHB1 vector which contains rhEGF gene controlled by T7 promoter. The transformant with pYHB101 showed relatively slow growth pattern compared to the transformant with pYHB1. However, we observed that the transformant with pYHB101 secreted rhEGF of 13 mg/l significantly after 5 hr induction with 1 mM IPTG and that the T7 promoter was more effective than tac promoter when connected to pelB signal sequence. The amount of rhEGF was 14 mg/l under the sub-optimized condition.