성격의 변화를 수반한 Wilson씨병 1례

유세종,이상렬,박민철 圓光大學校 醫科大學 神經精神科學敎室 1994 圓光精神醫學 Vol.10 No.2

We had a 22 year old male patient with Wilson's disease showing neuropsychiatric symptoms. He showed neuropsychiatric symptoms such as personality change, regressed and childish behavior, wing-beating tremor and rigidity on the upper extrmity, open mouth, drooling, bradykinesia, dysarthria and dysphagia. In brain MRI findings, bilateral high signal intensity on T2WIs were observed in basal ganglia, thalamus, central midbrain and periaqueductal pons. In liver scan, splenomegaly was detected. At a slit-lamp examination, we detected the brownish colored pigments(Kayser-Fleisher ring) on Descement's membrain of both eye corneas. Laboratory findings were a low serum ceruloplasmin(2.63㎎/dl, normal: 25~45㎎/dl), low serum copper level(32㎍/dl, normal: 80~130㎍/dl), and an increased 24 hous urine-copper excretion level(258㎍/day, normal: 15~30㎍/day). Therefore, we diagnose the patient as having wilson's disease and we started medication treatment with penicillamine.

Escherichia coli에서 발현된 재조합 인간 상피세포 증식인자의 정제 및 특성

박세철,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

E. coli BL21(DE3, pYHB101)을 이용한 rhEGF의 최적 생산조건은 25℃, 48시간 배양에서 변형된 MBL 배지에 glucose를 10g /l 첨가한 배지로 배양한 경우이었고 유도물질로 1mM IPTG를 사용하여 2시간 배양 후에 유도 배양하였을 때 최대 rhEGF가 68.7 mg/l가 발현되었다. 배양액으로부터 Amberlite XAD-7, ultrafiltration, DEAE Sepharose column chromatography 정제 과정을 거쳐 rhEGF가 정제되었으며 이때 정제도는 267배이었으며 66.6%의 회수율을 보였다. 정제된 rhEGF는 HPLC 분석 결과 2개의 분획으로 나누어졌으며 N말단 아미노산 서열 분석결과 Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His로 나타났다. 또한 5'-bromo-2'-deoxy-uridine (BrdU)의 삽입에 의한 DNA 생성능을 조사하였다. 상기의 결과로 E. coli BL21(DE3, pYHB101)에 의하여 생산된 rhEGF는 nhEGF와 동일한 것으로 추정되었다. Recombinant human epidermal growth factor (rhEGF) was produced strain was cultured at 25℃ for 48 hours in the modified MBS medium containing 10 g/l glucose with 1 mM IPTG induction at 2 hours after inoculation. The rhEGF was purified upto 267 folds by Amberlite XAD-7 chromatography, ultrafiltration, and DEAE Sepharose fast fow ion exchange chromatography with an overall yield of 66.6%. The purfied rhEGF was further separated into two fractions by HPLC. The N-terminal amino acid sequence of the second fraction was Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His. The effect of rhEGF on the DNA synthesis was examined using in vitro biological assay based on the incorporation of 5'-bromo-2'-deoxy-uridine (BrdU). The purified rhEGF shows no difference with natural human epidermal growth factor (nhEGF) in N-terminal amino acids residues and biological activity. From the results, we concluded that rhEGF produced from E. coli harboring the plasmid pYHB101 was apparently the same as nhEGF.

교통사고로 발생한 대량 환자의 중증도 분류와 이송의 분석

오세현,정용택,김병철,이부수 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Triage and transportations are important and life-saving medical task performed at the site of mutiple-casualty incidents(MCIs). We pursued three road traffic accidents victims to find out any problem in triaging and transporting them, and to provide an information for equipping an local disaster planning. Methods: The medical records of 70 consecutive patients who were transported to five emergency facilities in Kangnung, on three MCIs from 1998 to 2000, were analysed, retrospectively. The intervals and types of transport vehicles were analysed. We compared the prehospital time between severity-based groups, which were graded into 3 groups according to the Triage score(0,1~8,9). Results: Among the 70 patients, 33 patients(47.1%), 21 patients(30%), and 16 patients(22.9%) were transported to Level I, Level II, and Level III trauma centers, respectively. The time elapsed from scenes to the Level I trauma center was significantly shorter(p=0.003) than to others, but no differences in the mean Triage score between trauma centers were seen(p>0.05). The 119 rescue services transported 58.6%(41 patients) of victims to emergency facilities, and 58.5%(24 patients) of them was concentrated to Level I. There were no significant differences in the prehospital time between three Triage score groups(p>0.05). Conclusion: There was no medical control such as triage and transportation in the cases of three MCIs. In the construction of emergency medical service system(EMSS) preparing MCIs or disasters, we suggest the integration and unification of 119 rescue services and emergency medical information centers for effective medical control. We propose to making a new and simple triage guideline. The prehospital personnel should be educated and disaster drills should be performed under the guideline.

요크셔테리어견에서 발생한 기능성 양측성 난소 과립막세포종

오승준,정성목,김명철 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

A 12-year-o1d, intact female Yorkshire terrier was presented with a vulvar enlargement and progressive vaginal discharge purulently for 3 weeks. On radiographic, ultrasonographic and computed tomographic evaluation, a large mass was detected in left upper abdomen which measured about 3x4 centimeters. And a lesser large mass was detected in right upper abdomen and it had several discrete anechoic follicles and large cysts. Moreover, an enlargement of uterus was detected in a caudal abdomen. The masses and the uterus were removed by complete ovariohysterectomy and the resected specimen was histopathologically examined. Based on the gross, histopathologic findings, and the hormonal test, the masses were definitely diagnosed as bilateral functional malignant granulosa cell tumor (GCT). Until now, the dog has been successfully managed more than 4 months without complications.

임신부 뇨로부터 정제된 인간 상피세포 증식 인자 유사체의 in vitro bioassay 및 특성

박세철,전재현,남정현,권태종,고인영,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

벤조산흡착, 음이온 교환수지, 단일클론항체을 이용한 immunoaffinity chromatography를 통하여 임신부 뇨로부터 천연의 hEGF를 정제하였다. 정제된 hEGF는 μ Bonda C_18 column을 사용한 HPLC 분석을 통하여 4개의 fraction으로 분리가 가능하였으며 western blot과 double immunodiffusion 실험 결과, 각각의 fraction이 hEGF의 특성을 가진 유사체인 것을 알 수 있었다. 또한 hEGF 표준 물질과의 spiking 및 아미노산 분석 등을 통하여 두 번째 fraction이 nhEGF와 동일한 것으로 확인하였다. nhEGF 및 그 유사체의 생물학적 활성 비교를 위하여 NIH 3T3 세포주에서 5'-Brdu incorporation 측정을 위한 labelling 시간, 혈청 농도의 최적 조건을 결정하였다. NIH 3T3 세포주의 DNA 합성능은 0.2% FCS가 포함된 저혈청 배지에서 hEGF가 0.1~10 ng/ml 농도로 첨가하였을 때 증가하는 경향을 나타냈다. HPLC를 통하여 분리된 두번째 hEGF 유사체가 다른 유사체보다도 생물학적인 활성이 우수하였으며, rhEGF 표준물질과의 spiking 및 아미노산 서열 분석등을 통하여 nhEGF로 밝혀졌다. 임신부의 뇨의 hEGF 유사체 함량중 natural hEGF는 46%이었다. Natural human epidermal growth facto (nhEGF) was purified from pregnant human urine by benzoic acid adsorption, DEAE-Sepharose ion exchange, and immunoaffinity chromatography. The purified nhEGF was further separated into four fractions using Bondapak C_18 HPLC system. Following characterization by Western blot and double immunodiffusion, we found that each fraction corresponds to four derivatives of the nhEGF. For biological analysis of nhEGF, we optimized the labeling time and serum concentration for the incorporation of 5-bromo-2'-deoxy-uridine (BrdU), a non-radioactive alternative for [^3H]-thymidine uptake, into NIH 3T3 cells. The DNA synthesis of NIH 3T3 cells was gradually increased at the nhEGF concentrations between 0.1~10 ng/ml in the Dulbecco's Modified Eagles Medium (DMEM) containing 0.2% Fetal calf serum (FCS). When we assayed the biological activity of four fractions, the activity of the second fraction was superior to that of the others.

밀폐에 의한 기계설비 소음의 감쇠 예측

윤세철,오종민 경희대학교 환경연구소 1997 環境硏究 論文集 Vol.7 No.-

The considerations in the noise control by enclosure are the rise of temperature and sound pressure, transmission loss, absorption coefficient of the materials, the structure of the soundproof panels, an opening and coincidence frequency. But it is very difficult that we obtain the accurate data about those in design, so, the noise reduction after enclosing does not correspond. The difference of the noise reduction between the calculation and the measurement was average 4.8dB

환경불확실성에 따른 품질경영 활동과 경영성과에 관한 연구

정상철,오세구 충남대학교 경상대학부설 경영경제연구소 1997 경영논집 Vol.13 No.2

본 연구는 품질경영에 대한 이론적 배경을 고찰하고, 우리나라 제조업체의 실질적인 품질 경영활동과 경영성과간의 관계에 대하여 분석하였다. 본 연구는 기존의 대다수의 연구들이 취하던 품질경영의 성과측정에 그치지 않고 변수들간의 구조분석을 통하여 실제 어떤 활동이 성과에 주된 역할을 하는지에 대하여 살펴보았다. 실증분석결과 동기부여, 신기술개발등이 재무성과, 비재무성과에 공통적으로 큰 영향을 미치는 활동으로 확인되었다.

주택형 태양광발전시설의 전압품질 분석

김세호,김철성 제주대학교 공과대학 첨단기술연구소 2005 尖端技術硏究所論文集 Vol.16 No.2

According to the economy growth and altitude of industrial society life, demands for the electricity enlarge We are faced with serious situation lacking of energy and changes surrounding the earth environment. Related experts commonly have agreed that strong measures must be taken to reserve long-term stability of electricity supply. Meanwhile, solar photovoltatic power generation is drawing most attention from the experts. Because this system uses unlimited and unpolluted solar energy, It doesn't need costs of fuel and doesn't have no air pollution and no waste matter. The paper investigates the influences of Solar photovoltatic power generation system on voltage and harmonics

공동주택 바닥충격음 차단성능 평가

윤세철,오종민 慶熙大學校 地球環境硏究所 2002 지구환경논문집 Vol.13 No.-

In apartment buildings, floor-impact sound has been regarded as the major source which induces complaints from residents. It is mainly due to the use of light-weight structures and the lack of researches in terms of the sound transmission characteristics through the structures of a receiving room. The vibration produced by the impact on one part of an apartment building would travel as far as the other parts of structure only with with a little alleviation. As a result, the impact sound from upstairs has been regarded as a main source of noise causing discontentment among occupants. This study was carried out to measure the floor-impact sound levels and evaluate the insulation performance of floor-impact sound for the K apartment buildings which complaints induced and the newly-founded Y apartment buildings. The major results of this study are as followers; 1. The comparison showed that the light-impact sound levels and the levels of estimated light-impact sound ranged from 51.0dB to 73.9dB and 60dB to 66dB in the K apartment buildings, 50.2dB to 70.6dB and 57dB to 62dB in the Y apartment buildings. 2. The levels of estimated impact sound exceed the criteria of light-impact noise insulation 58dB by 6∼8dB and the criteria of heavy-impact noise insulation 50dB by 4∼6dB in the K apartment buildings and 4dB, 1dB in the Y apartment buildings, respectively.

시차주사열량분석기를 이용한 치과용 접착시멘트의 경화기전 및 반응열에 관한 연구

김철위,이용근,윤태호,김효종,남세진 大韓齒科器材學會 2000 대한치과재료학회지 Vol.27 No.3

The working time of dental cement has a close relation with the setting time, where the setting reaction can be said as the result of the chemical reaction between cement liquid and powder. The purpose of this experiment was to investigate the setting characteristics and setting rate of dental cements by measuring the exothermic heat(Joule/gram) from the setting reaction at specified times using a differential scanning calorimeter (Differential Scanning Calorimeter, DSC 204, TASC 414/3A Controller, Netzche, Germany). Graphs of time-exothermic heat at 37℃ isothermal condition, showing the setting reaction of dental cements were plotted. Three types of luting cements studied were zinc-phosphate cement (ZPC), polycarboxylate cement (PCC), and glass ionomer cement (GIC). The effects of powder-liquid ratio on the setting characteristics of cements were also included. The amount of heat (calory) released per one gram of cement was calculated at each specified time, and was analyzed. ANOVA and Scheff's multiple comparison test (p=0.05) were used for statistical analysis using SPSS/PC+. The setting rate of cement was not constant during the setting reaction, and different setting characteristics were observed depending on the type of cement and powder/liquid ratio. Generally, 70∼90% of the setting reaction, measured by the exothermic heat release, occurred within 5 minutes after mixing. The exothermic heat released by ZPC was very high immediately after mixing, and the duration of the heat release was prolonged compared with other cements. The exothermic heat release of PCC finished most rapidly. It was generally observed that the amount of heat release (rise in temperature) per one gram of cement was highest for ZPC, followed by GIC, and then PCC. However, heat release during the first 2∼5 minutes after mixing was highest for GIC, followed by ZPC and then PCC. According to the Scheff's multiple comparison test, the duration of the total setting reaction did not show any significant difference among the cements(p>0.05), and also among the different power/liquid ratio groups(p>0.05). It was observed that more heat was released with the lower powder/liquid ratio than the higher powder/liquid ratio.