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Harris, Sarina G.,Shuler, Michael L. The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4
The blood-brain barrier (BBB) is composed of the brain capillaries, which are lined by endothelial cells displaying extremely tight intercellular junctions. Several attempts at creating an in vitro model of the BBB have been met with moderate success as brain capillary endothelial cells lose their barrier properties when isolated in cell culture. This may be due to a lack of recreation of the in vivo endothelial cellular environment in these models, including nearly constant contact with astrocyte foot processes. This work is motivated by the hypothesis that growing endothelial cells on one side of an ultra-thin, highly porous membrane and differentiating astrocyte or astrogliomal cells on the opposite side will lead to a higher degree of interaction between the two cell types and therefore to an improved model. Here we describe our initial efforts towards testing this hypothesis including a procedure for membrane fabrication and methods for culturing endothelial cells on these membranes. We have fabricated a 1 $\mu\textrm{m}$ thick, 2.0 $\mu\textrm{m}$ pore size, and 55% porous membrane with a very narrow pore size distribution from low-stress silicon nitride (SiN) utilizing techniques from the microelectronics industry. We have developed a base, acid, autoclave routine that prepares the membranes for cell culture both by cleaning residual fabrication chemicals from the surface and by increasing the hydrophilicity of the membranes (confirmed by contact angle measurements). Gelatin, fibronectin, and a 50/50 mixture of the two proteins were evaluated as potential basement membrane protein treatments prior to membrane cell seeding. All three treatments support adequate attachment and growth on the membranes compared to the control.
사리나 ( Sarina ) 청람어문교육학회(구 청람어문학회) 2018 청람어문교육 Vol.0 No.65
본고는 우선 사전별로 제시하는 ‘-에서’의 용법을 바탕으로 본 연구에서 다루는 ‘-에서’의 용법을 구분한다. 그 다음에 현재 한국어 교재에서 ‘-에서’에 대해 기술한 문제점을 비판적으로 검토한 후 교재에서의 ‘-에서’ 용법의 출현여부 및 말뭉치 출현 빈도에 근거하여 ‘-에서’의 용법을 선정하는 것이다. 조사 ‘-에서’의 사전별 용법을 살펴본 결과에 근거하여 ‘-에서’의 용법이 9개로 분류를 하여 교재 분석 및 말뭉치 분석을 진행하였다. 말뭉치 분석은 말뭉치에서 검색된 문장 분석을 통하여 ‘-에서’의 용법 사용 빈도를 조사하였다. 이를 기준으로 한국어 교육용 ‘-에서’의 용법을 선정하였다. 본 논문은 다른 연구와 달리 기존에 있는 사전의 용법들을 검토하고 ‘-에서’의 코퍼스 빈도, 교재 출현을 기준으로 하여 최종적으로 한국어 교육에 필요한 ‘-에서’의 용법을 선정을 하는 데에 의의기 있다. 본 연구는 향후에 한국어 교육에 조금이나마 이바지할 것을 기대한다. This paper distinguishes the usage of ‘-eseo’. This study based on the usage of ‘-eseo’, which is presented first by dictionary. Then, after critically reviewing the problems described in the current Korean textbook ‘-eseo’, the usage of ‘-eseo’ is selected based on the appearance of ‘-eseo’ in the textbook and the frequency of occurrence of corpus. Based on the results of examining the dictionary, the usage of ‘-eseo’ was categorized into 9, and text analysis and corpus analysis were conducted. The corpus analysis was conducted by analyzing sentences in corpus. Based on this, the usage of ‘-eseo’ for Korean language education was selected. In this paper, we review the existing dictionary's usage and compare the corpus frequency and the appearance of the textbooks. This study is expected to contribute a little to Korean language education in the future.
Maternal and Infantile Mortality in Selangor
Azlizan Mat Enh,Sarina Abdullah 숙명여자대학교 아시아여성연구원 2012 Asian Women Vol.28 No.1
The health of mothers and infants, particularly in Malay States during the region’s colonization, has not been extensively studied by historians. This study thus aims to analyze the pattern and causes of maternal and infantile mortality rates in Selangor during the colonial era from 1900 to 1940. This period covers the mass arrival of Chinese and Indian immigrants in Selangor. The study examined the three main ethnic populations in Selangor: Malays, Chinese, and Indians. The data used were obtained from an analysis of primary sources, especially from the health records of the Selangor Health Department in the national archives of Malaysia. The study found that the rate of increase in maternal and infantile mortality in Selangor during that period was caused by various factors, including the unplanned system of urbanization that led to population congestion, the lack of basic health facilities, poverty, the lack of knowledge about health care, diets followed during and after pregnancy and, finally, cultural practices inhibiting certain ethnicities from receiving modern treatment from maternity hospitals.
Michael L. Shuler,Sarina G. Harris 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4
The blood-brain barrier (BBB) is composed of the brain capillaries, which are lined by endothelial cells displaying extremely tight intercellular junctions. Several attempts at creating an in vitro model of the BBB have been met with moderate success as brain capillary endothelial cells lose their barrier properties when isolated in cell culture. This may be due to a lack of recreation of the in vivo endothelial cellular environment in these models, including nearly constant contact with astrocyte foot processes. This work is motivated by the hypothesis that growing endothelial cells on one side of an ultra-thin, highly porous membrane and differentiating astrocyte or astrogliomal cells on the opposite side will lead to a higher degree of interaction between the two cell types and therefore to an improved model. Here we describe our initial efforts towards testing this hypothesis including a procedure for membrane fabrication and methods for culturing endothelial cells on these membranes. We have fabricated a 1 mm thick, 2.0 mm pore size, and ~55% porous membrane with a very narrow pore size distribution from low-stress silicon nitride (SiN) utilizing techniques from the microelectronics industry. We have developed a base, acid, autoclave routine that prepares the membranes for cell culture both by cleaning residual fabrication chemicals from the surface and by increasing the hydrophilicity of the membranes (confirmed by contact angle measurements). Gelatin, fibronectin, and a 50/50 mixture of the two proteins were evaluated as potential basement membrane protein treatments prior to membrane cell seeding. All three treatments support adequate attachment and growth on the membranes compared to the control.
Lower blepharoplasty: how we do it
Chuan-Hsiang Kao,Sarina Rajbhandari 대한미용의학회 2019 대한미용의학회지 Vol.3 No.2
The natural process of ageing causes multiple age-related changes, which are observed in the anatomy of the eyelids and surrounding structures, including the malar region. Blepharoplasty is a procedure performed widely for tired looking eyes and facial rejuvenation. Lower blepharoplasty (LBP) is usually done with fat transposition rather than fat resection to avoid hollow appearance of malar area. Patients with excess fat and no tear trough deformity can be treated with fat resection alone, where as those with a prominent tear trough deformity requires fat transposition. Transposing the medial and central fat pads instead of excising them can help to fill out the hollowness of the under eye area whereas the lateral fat pads are removed as much as needed through direct excision. In our practice, we generally prefer a transconjunctival approach to lower lid blepharoplasty with fat repositioning.