Phenolic composition, enzyme inhibitory, and antioxidant activity of Bituminaria bituminosa

Sarikurkcu, Cengiz,Cengiz, Mustafa,Uren, Mehmet Cemil,Ceylan, Olcay,Orenc, Tuba,Tepe, Bektas 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This study aimed to evaluate the in vitro antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of Bituminaria bituminosa. In phosphomolybdenum assay, the methanol extract showed the highest activity ($166.78{\mu}mol\;TEs/g$ dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl ($DPPH^{\bullet}$) and 2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid) ($ABTS^{{\bullet}+}$). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and $46.82{\mu}mol\;TEs/g$ dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, ${\alpha}$-glucosidase inhibition assay resulted in the superiority of water extract ($1233.86{\mu}mol\;ACEs/g$ dry plant). In the case of ${\alpha}$-amylase inhibitory assay, the ethyl acetate extract showed the highest activity ($53.65{\mu}mol\;ACEs/g$ dry plant). The water extract exhibited the highest phenolic content ($31.70{\mu}mol\;GAEs/g$ dry plant). In contrast, the methanol extract was found rich in flavonoid compounds ($5.29{\mu}mol\;REs/g$ dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.

Chemical Composition, Antioxidant, and Enzyme Inhibitory Activities of the Essential Oils of Three Phlomis Species as well as Their Fatty Acid Compositions

Cengiz Sarikurkcu,Mehmet Cemil Uren,Mehmet Sefa Kocak,Mustafa Cengiz,Bektas Tepe 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

This study aimed to investigate the chemical composition, antioxidant, and enzyme inhibitory activities of the essential oils of Phlomis armeniaca WILLD., P. nissolii L., and P. pungens WILLD. var. pungens as well as their fatty acid contents. Germacrene D was found as the major compound in the oils (24.7, 15.1, and 7.2%, respectively). Additionally, n-hexadecanoic acid, hexahydrofarnesyl acetone, β-caryophyllene, and linalool were the other main compounds in the oils. Among the fatty acids, C18:3 ω3 and C18:2 ω6 were determined in high quantities in P. armeniaca (23.14 and 18.01%, respectively) and P. pungens var. pungens (24.64 and 17.51%, respectively). The essential oils of P. armeniaca and P. pungens var. pungens showed remarkable antioxidant and metal chelating activities as well as great reducing power potentials. These oils also showed a moderate scavenging effect on ABTS radicals. The oils also exhibited various degrees of inhibitory activities on AChE, BChE, α-amylase, α-glucosidase, and tyrosinase.

Phenolic composition, enzyme inhibitory, and antioxidant activity of Bituminaria bituminosa

Cengiz Sarikurkcu,Mustafa Cengiz,Mehmet Cemil Uren,Olcay Ceylan,Tuba Orenc,Bektas Tepe 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.5

This study aimed to evaluate the in vitro antioxidant and enzyme inhibitory activities of ethyl acetate, methanol, and water extracts of Bituminaria bituminosa. In phosphomolybdenum assay, the methanol extract showed the highest activity (166.78 μmol TEs/g dry plant). The water extract exhibited the highest scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH•) and 2,2-azino-bis (3- ethylbenzothiazloine-6-sulphonic acid) (ABTS•+). In addition, it exhibited the highest activity in cupric ion reducing (CUPRAC) and ferric reducing antioxidant power (FRAP) assays (41.26 and 46.82 μmol TEs/g dry plant). The extracts did not show cholinesterase and tyrosinase inhibitory activity. However, α-glucosidase inhibition assay resulted in the superiority of water extract (1233.86 μmol ACEs/g dry plant). In the case of α-amylase inhibitory assay, the ethyl acetate extract showed the highest activity (53.65 μmol ACEs/g dry plant). The water extract exhibited the highest phenolic content (31.70 μmol GAEs/g dry plant). In contrast, the methanol extract was found rich in flavonoid compounds (5.29 μmol REs/g dry plant). The water extract contained considerable amounts of rosmarinic acid, luteolin, quercetin, and rutin. Therefore, it can be used as a source of new and alternative antioxidant and enzyme inhibitory agents.

A comparative study on the phenolic composition, antioxidant and enzyme inhibition activities of two endemic <i>Onosma</i> species

Saravanakumar, Kandasamy,Sarikurkcu, Cengiz,Sarikurkcu, Rifat Tayyib,Wang, Myeong-Hyeon Elsevier 2019 Industrial crops and products Vol.142 No.-

<P><B>Abstract</B></P> <P>The studies on bioactivities of plant extracts are a fundamental requirement for future pharmacological research. Therefore, the present study extracted the phytochemicals from the two endemic species such as <I>Onosma isaurica</I> and <I>O. bracteosa</I> and tested their antioxidant, reducing power and enzyme inhibitory activities followed by scanning of the phytochemicals in the extracts by using liquid chromatography–electrospray tandem mass spectrometry (LC–ESI–MS/MS<I>)</I>. The results revealed that the antioxidant activity in terms of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and ferrous ion chelating was not statistically significant between the species but 2,2-azino-bis (3-ethylbenzothiazloine-6-sulphonic acid) (ABTS) radical scavenging was significant between the two species. Reducing power in terms of ferric reducing antioxidant power (FRAP) reducing did not exhibit the significance while cupric ion (CUPRAC) reducing and phosphomolybdenum displayed significance between the two species. In case of the enzyme inhibitory assay, the α-amylase inhibitory activity was significant but tyrosinase inhibitory was not significant between the species. The bioactivities of the extracts were compared with standard positive controls as trolox for ABTS radical, DPPH radical, FRAP reducing, CUPRAC reducing and phosphomolybdenum; Ethylenediaminetetraacetic acid (EDTA) for ferrous ion chelating; acarbose for α-amylase inhibition; and kojic acid for tyrosinase inhibition. Both of the species showed significantly higher bioactivity than their respective positive controls. Interestingly the bioactivity was found promising with <I>O. bracteosa</I> over <I>O. isaurica</I> due to the high presence of the phenolics. The richness of the phytochemicals in <I>O. bracteosa</I> was evidenced by LC–ESI–MS/MS analysis. In conclusion, the two endemic species - <I>O. isaurica</I> and <I>O. bracteosa</I> – which are rich in bioactivity deserve for conservation and sustainable utilization towards developing pharmaceutically valid natural products in the future.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The bioactive phytochemicals in two endemic <I>Onosma</I> species was examined. </LI> <LI> The extracts from both <I>Onosma</I> species showed the potent health promoting activities. </LI> <LI> The phytochemicals in extracts was identified by LC–ESI–MS/MS analysis. </LI> <LI> Conservation of these endemic species can offer the pharmaceutically valid products. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Evaluation of Antioxidant Activities of 3 Edible Mushrooms: Ramaria flava (Schaef.: Fr.) Quél., Rhizopogon roseolus (Corda) T.M. Fries., and Russula delica Fr.

Nevcihan Gursoy,Cengiz Sarikurkcu,M. Halil Solak,Bektas Tepe 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.3

The methanolic extracts of Ramaria flava,Rhizopogon roseolus, and Russula delica were analyzed for their antioxidant activities in different test systems including β-carotene/linoleic acid, 1.1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, reducing power, and metal chelating activities in addition to their total phenolic and flavonoid contents. In the first case,methanol extract of R. roseolus showed the strongest activity. In DPPH system, the scavenging effects increased with the concentration. The reducing power of the mushroom also increased with concentration. Chelating effect was 96.75±0.28% for R. flava. In the case of total phenolic and flavonoid assays, R. flava found to have the highest phenolic content. Total flavonoid content of R. flava again found the superior to the other mushrooms. Experimental results indicate that the mushroom species evaluated here can be consumed safely. On the other hand, knowing the biological activity of these mushrooms will contribute to the establishment of conscious consumption.

Essential Oil Composition and Antioxidant Activities of Alkanet (Alkanna tinctoria subsp. tinctoria)

M. Sabih Ozer,Cengiz Sarikurkcu,Sendil Can,Bektas Tepe 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.5

This study is designed to examine the chemical composition and in vitro antioxidant activity of the hydrodistillated essential oil and the various extracts of alkanet (Alkanna tinctoria subsp. tinctoria). Gas chromatography (GC) and GC-mass spectrometry (GC-MS) analysis of the essential oil were resulted in the determination of 27different compounds, representing 93.32% of the total oil. The major compounds detected in the oil, were pulegone (22.27%), 1,8-cineole (13.03%), α-terpinyl acetate (6.87%),and isophytol (6.83%), respectively. Antioxidant activities of the samples were determined by 4 different test systems namely β-carotene/linoleic acid, 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power, and chelating effect. In all systems, essential oil showed the weakest activity profile. On the other hand, ethyl acetate and water extracts exhibited excellent antioxidant activities. As well as the antioxidant activities of the extracts, they were evaluated in terms of their total phenolic and flavonoid contents. In parallel to the experiments, ethyl acetate and water extracts were found to be rich-in these phytochemicals.