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Sanzhen Liu,Yao Li,Xuping Fu,Minyan Qiu,Bin Jiang,H ai W u,Rongyu Li,Yumin Mao,Yi Xie 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2
The oligonucleotide microarray, a high-throughput polymorphism detection technology, holds great promise for the characterization of complex genetic variance. To achieve greater sensitivity and specificity for it to be an effective platform technology we present results and discuss some of the factors influencing signal intensities and single-mismatch discrimination in array-based mutation/SNP detection. Probes with a series of concentrations were spotted onto the slide in order to find the optimal concentration with the identifiable satisfying signals and the stable ratios between matched and mismatched probes. It was found that under our experimental conditions, when the initial probe concentration is higher than the maximum immobilization capability of the slide (7.5 µM), the hybridization signal will be saturated and the ratio between matched and mismatched probes will be more stable than at a lower probe concen-tration. Considering the cost of probes and the systematic stability, a constant spotting cone n-tration of 10 µM was selected. The stability of different types of mismatched oligo-DNA du-plexes on the glass surface was also confirmed. The results show that the order of stability of mismatched oligo-DNA duplexes on a glass surface is in general agreement with previous reports conducted using liquid and polyacryl-amide gel pads. This suggests that the influence of the mismatched base pair on the stability of the duplex in a solid hybridization system is similar to that in the solution hybridization environment.
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Wei, Qing,Liu, Sanzhen,Huang, Jianfeng,Mao, Xueying,Chu, Xiaohui,Wang, Yu,Qiu, Minyan,Mao, Yumin,Xie, Yi,Li, Yao Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.4
Double stranded targets on the cDNA microarray contain representatives of both the coding and noncoding strands, which will introduce hybridization competition with probes. Here, the effect of double and single strands of targets on the signal intensity and the ratios of Cy5/Cy3 within the same slide were compared. The results show that single stranded targets can increase the hybridization efficiency without changing the Cy5/Cy3 ratio. Based on these results, a new strategy was established by generating cDNA targets with asymmetric PCR, instead of conventional PCR, to increase the sensitivity of the cDNA microarray. Furthermore, the feasibility of this approach was validated. The results indicate that the cDNA microarray system based on asymmetric PCR is more sensitive, with no decrease in the reliability and reproducibility as compared with that based on conventional symmetric PCR.
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Jilai Zeng,Zailun Liu,Hui Quan,Ancan Shao,Sanzhen Wang 한국유체기계학회 2022 International journal of fluid machinery and syste Vol.15 No.1
The pressure distribution law and calculation of impeller side cavity is one of the important topics in the pump industry, but there is no more accurate calculation method at present. On this basis, a mathematical model of pump cavity pressure distribution and balance cavity pressure under design conditions was derived in this paper. By changing the radial clearance of front and back sealing rings and the diameter of the balance hole on the same impeller, the pressure distribution of front and rear cavities and the balance cavity pressure of the pump were systematically tested by a specially designed device. In the test, the curve of pressure loss coefficient and relative radius of pump cavity under different sealing ring radial clearance was obtained, which could solve the calculation problem of the pressure distribution of front and rear pump cavities. The test curve of the relationship between flow coefficient ratio and specific surface area essentially reflected the coupling regulation effect of balance holes and the rear sealing ring on the balance cavity pressure, which could not only solve the calculation problem of the balance cavity pressure, but also provide the theoretical basis for controlling the pressure and cover force of balance cavity and select different combinations of the radial clearance of the rear sealing ring and the diameter of balance holes. The results showed that the theoretical prediction value was more consistent with the actual measured value, which proved that the proposed mathematical model has high accuracy and universality. This study provides a new way to more accurately calculate the pump cavity pressure distribution and balance cavity pressure and laid a theoretical basis for the study of impeller cover force with significant academic and application value.
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