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토끼 신장 근위세뇨관 일차 배양 세포에서 Bradykinin의 Phospholipase D(PLD)에 의한 신호 전달 기전
정성현,박경협,정지창 慶熙大學校 1993 論文集 Vol.22 No.-
Recently, phospholipase D (PLD) is known to be involved in several bioactive materials-mediated signal transduction pathway. In order to investigate how bradykinin works in renal tissue, we in this study wants to figure out whether bradykinin mediates its physiological actions through activation of PLD or not. Bradykinin, which is a well known neuroactive peptide and plays many important physiological roles as vasodilatation, hyperalgia, has been reported to activate PLD in PC12 cells. As a corollary of our experimental results, we are first group ever to reveal the positive involvement of PLD in bradykinin-mediated signal transduction mechanism in the rabbit kidney proximal tubule cells.
이상협;배형철;송석태;한찬수;채희백 순천향대학교 기초과학연구소 2010 순천향자연과학연구 논문집 Vol.16 No.2
In order to know how the [2-(2'-ethylhexyloxy)-5-methoxy-1,4-phenylenevinylene] (MEH-PPV) layer works in organic light emitting diode, two different types of devices were fabricated, which are MEH-PPV single layer devices and MEH-PPV/tris (8-hydroxy-quinoline)aluminium(Alq3) bilayer devices. On the indium-tin-oxide(ITO) glass anode MEH-PPV layer was first spin-coated, and the Alq3 layer and the aluminium(Al) anode were vapor-deposited sequentially. The devices shows a trend that has lower current, luminous efficiency in case the thickness of the device is larger. Some bilayer devices, however, show more improved performance than other devices of smaller total thickness. This results were discussed on the basis of improved exciton formation resulting from balanced carrier transport.
기능성 소화불량증 환자에 대한 반하사심탕 엑기스제의 임상효과
윤상협,류봉하,류기원,김진성 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2003 東西醫學硏究所 論文集 Vol.2003 No.-
Objective : We evaluated the therapeutic effect of Banwhasasimtang dry extract powder on gastrointestinal symptoms and its side effects in patients with functional dyspepsia. Methods : Patients with functional dyspepsia, defined as discomfort in the epigastrium. A total 45 patients participated participated in this study. Dyspeptic symptoms ratio was obtained by patients, total point of symptom score / total 21 points x 100(%). On each of symptom after 4 weeks administration of Banwhasashim-tang was made by variation of symptom score: If symptoms score of posttreatment is lower than that of pretreatment, It is improved ; Otherwise aggravated, and if there is no change between them, unchangeable. When a full health condition of normal control is assumed 100%, relative health condition of dyspeptic patient could be calculated from 'full health condition- dyspeptic symptoms ratio. Therefore a whole satisfaction rate on symptom improvement was presented with variation of a relative health condition to dyspepsia between pretreatment and post. Results : Banwhasashimtang significantly unproved symptoms in patients. The significance was shown in both all seven items(p< 0.01, respectively) and 19.2% (p<0.01) of increase of a whole symptom improvement. There is no remarkable side-effect in all laboratory test, excepting 1 case abdominal pain another one case of recurred pain of chronic prostatitis. Conclusion : Banwhasashimtang extract provides symptomatic relief in functional dyspepsia and is no remarkable side-effect drug.
김성희,류봉하,류기원,윤상협,김진성 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2002 東西醫學硏究所 論文集 Vol.2002 No.-
Background Nowadays a lot of reseant is based on natural substances or materinals world wide since many kinds of side effects are accompanied by anti tumor chemotherapy. In Chinese medicine, Dioscorea bulbifera L is widely used to treat many kinds ofi cancer, but in Korea it is rarely used. Therefore, we need to scientifically identify anti tumor effects of Dioscorea bulbifera L.Objective: We aimed to identify anti tumor effects of Discorea bulbifera L on the stomach cancer cells through molecular biological methods.Materials & Methods: We used AGS, a stomach cancer cell from American Type Culture Collection We injected the boiled extrat of Dioscorea bulbifera L 5 ul(sample 1), 10u1(sample 2) to cultured media(ml) for 0,6,12,15,24 hours. We measured the killing effect on stomach cancer cells through Tryphan blue exclusion test and suppressive effect on viability of stomach cancer cells via MTT assay Results: Tryphan blue exclusion test showed that each test group killed more stomach cancer cells than the controlled group with a dosage-dependent, but not significantly. MTT assay showed that each test group had a more suppressive effect on viability of stomach cancer cells than the controlled group without a dosage-independent, but not significantly.The cell cycle analysis via flow cytometry showed that the test group extended cell cycle, and there was no peak in M phase, the number of subGl , GO, G1 phase cells increased a little, but not significantly.Conclusion : This experiment showed that Dioscorea bulbifera L. has an anti-tumor effect, but not significantly. This is in vitro experiment and basic experiment on Dioscorea bulbifera L. We hope more progressive researches on Dioscorea bulbifera L. will be conducted and its anit tumor will be more accurately identified.
윤상협,이종수 경희대학교 동서의학연구소 2001 東西醫學硏究所 論文集 Vol.2000 No.-
Objective : The aim of this study was to investigate the neuroprotection effect of estrogen on brain atrophy following cerebral infarction. Method : All animals in this study were classified into 4 groups; ovariectomy group (OVXgroup), cerebral infarction group (INF group), combination ovariectomy and cerebral infarction group ( OVX + INF group), and naturally intact group for control data (NOR group ). Cerebral infarction was made by Chen's method with some modification. Ovariectomy was performed by Wayforth's method. Experimental data for each group was collected at 15days, 1 month, 3 months, and 6 months after starting observation. Serum 17β-estradiol(E2) was determined by radioimmunoassay. Brain volume was measured and calculated with image analysis. Each brain was sliced at intervals of 2mm in chamber after 30 min of freezing in refregerater. Cerebral volume was obtained by sum of volume of each slice level, which was mean area × 2mm. Results : Cerebral ischemia was fou8nd to decrease the serum concentration of 17β-estradiol and to inhibit the physiologically conpensatary function of the ovariectomized rats. Also we found that deprivation of estrogen have resulted in more severe cerebral atrophy followed by cerebral infarction. Conclusion : It is suggested that estrogen has a neuroprotection effect on cerebral atrophy following cerebral infarction.(J Korean Oriental Med 2000;21(4):9-15)
윤상협 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 1997 東西醫學硏究所 論文集 Vol.1997 No.-
With the purpose of producing easily the basal ganglia infarction into Chen's, scerebral ischemic model which is almost cortical infarct made by the ligation of distal part of MCA and 1 hr obliteration of both common carotid arteries in rat, the MCA obstruction was extended between rhinal fissure and olfactory tract with electrocauterization in place of 10-0 silk suture ligation of distal part of MCA. Both original Chen's model and modified Chen's have shown the cortical infarction in dorsolateral & lateral frontoparietal cortex, but not any infarction in basal ganglia. However, the modified Chen's model have shown the effect of average 12% increase in cortical infarct than that of original Chen's model. This experimental results suggest the modified Chen's model can not reduce the blood flow of the lateral lenticulostriatal artery enough to make the basal ganglia infarction and that blood circulation of basal gagglia under its condition is probably being kept partly through the posterior cerebral artery via vertebral artery. Therefore, The follow-up observation on ischemic time lapse would be needed.