Effect of Platelet-Rich Fibrin on Repair of Defect in the Articular Disc in Rabbit Temporomandibular Joint by Platelet-Rich Fibrin

( Hyun Su Baek ),( Hye Sung Lee ),( Bok Joo Kim ),( In Kyo Chung ),( Chul Hoon Kim ),( Sun Mi Jin ),( Hie Sung Hwang ),( Sang Hun Shin2 ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.6

The objective of this study was to evaluate platelet-rich fibrin (PRF)’s effectiveness in repairing articular disc defect in the temporomandibular joint (TMJ) of rabbits. Eight rabbits were divided into four groups of two rabbits each, corresponding to groups A, B, C, and D. Both TMJs of all of the rabbits were used in the experiments: the right joints comprised the experimental groups, and the left ones, the control groups. The disc defect was circular and 2 mm in diameter. In the experimental groups, the PRF was compressed into the defect, whereas the control group defects were left untreated. A, B, C, and D groups were sacrificed at the 1st, 2nd, 4th and 6th weeks, respectively. The defects of each control group exhibited no specific changes. Contrastingly, in each experimental group, there was an increased number of chondroblasts at the margins of the defects, along with accelerated cell differentiation and a columnar cell arrangement observable at the time of cell differentiation. The experimental groups showed inflammatory cell infiltrations and fibrosis by the 1st week, maturation of chondrocytes by the 2nd week, and proliferation by the 4th week, after which the defects began to be filled with chondrocytes, a process that was complete after the 6th week. In the histological evaluation (H-E), the experimental groups showed significant increases of chondroblasts after the 2nd and 4th weeks, as well as regular columns of chondrocyte arrays observable during cell division. After 6 weeks, the defects were filled with chondrocytes.

실용음악 전공 학생들의 발성치료 전후 성대 및 음성 특성 비교

황연신,심미란,김철준,최용석,김상연,최찬호,선동일,Hwang, Yeon Shin,Shim, Mi Ran,Kim, Chul Jun,Choi, Yong Suk,Kim, Sang Yeon,Choi, Chan Ho,Sun, Dong Il 대한후두음성언어의학회 2016 대한후두음성언어의학회지 Vol.27 No.2

Background and Objectives : Many students major in applied music singing have various difficulties in phonation when they sing or perform. But the studies about this cause are lack. The purpose of this study is whether singing voice therapy based on vocal music is effective or not to applied music singing students that have difficulties in phonation. Materials and Methods : Singing voice therapy program had been done to 8 students major in applied music singing during 7 weeks. We did laryngeal stroboscope, perceptual, acoustic and aerodynamic examination before and after singing voice therapy to this students. And we studied post examination results have differences or improvements compared to pre examination results. Results : Four male students don't have effective improvement after singing voice therapy but they shows slight improvement in aerodynamic examination values compared to pre examination values. Four female students shows improvement in aerodynamic examination values and pitch range increasing. Above all, one student's vocal nodules disappeared after singing voice therapy. Conclusion : Singing voice therapy based on music singing is very effective to the students major in applied music singing. Above all, their curriculum in applied music singing should include phonation training based on music singing before training perform and technic practices.

<SUP>18</SUP>F-FDG PET-CT에서 진단된 갑상선 우연종의 악성종양 발견율 및 특징

이강영,고건<SUP>1<.SUP>,김선국<SUP>1<.SUP>,고진철<SUP>1<.SUP>,김행수,최상용,박신희,박용휘<SUP>2<.SUP>,Kang Young Rhee,Gun Koh,<SUP>1<.SUP>,Sun Kuk Kim,<SUP>1<.SUP>,Jin Chul Koh,<SUP>1<.SUP>,Haeng Soo Kim,Sang Yong Choi,Shin 대한갑상선-내분비외과학회 2008 The Koreran journal of Endocrine Surgery Vol.8 No.1

<B>Purpose: </B>PET-CT is often used to differentiate benign or malignant thyroid incidentalomas. In this retrospective study, we evaluated whether the <SUP>18</SUP>F-FDG uptake pattern and PET-CT findings improved accuracy over the standardized uptake value (SUV). <B>Methods:</B> <SUP>18</SUP>F-FDG PET-CT was performed on 2,178 subjects from August, 2004, to October, 2007, in Sung-ae Hospital. PET-CT was performed on 806 patients (37%) with suspected or known nonthyroidal cancer and 1,372 healthy subjects (63%) without a previous history of cancer. We investigated the clinical characteristics of patients, history, standardized uptake value (SUV), ultrasonography, and hormone levels in blood. Thyroidal cancer was confirmed by ultrasonography-guided fine needle aspiration and pathology after thyroid operation. <B>Results:</B> The prevalence of focal thyroid lesions on PET-CT was 8.8% (191/2178). Thyroid cancer confirmation was 7.9% (15/191). The maximum SUV of malignant thyroid lesions were significantly higher than that of benign lesions (7.00±3.08 vs. 4.49±1.84, P<0.001). <B>Conclusion:</B> PET-CT image interpretation that includes 18F-FDG uptake and SUV is better than PET-CT alone for differentiating benign and malignant lesions. Thyroid cancer risk increases as SUVmax levels increase. <B>(Ko</B><B></B><B>rean J Endocrine Surg 2008;8:38-42)</B>

Study and Application of the New Stick Make Up Product Using Clay Minerals as Binder & Buffer.

( Sang-je Kim ),( Dong-uk Shin ),( Pan-gu Cho ),( Chul-hee Jung ) 대한화장품학회 1999 대한화장품학회지 Vol.25 No.4

The new stick make-up product was studied by using a gel,, which is a viscous complex formed with clay minerals, vitamins A and E and fluorinated liquid polymer with a 1500 molecular weight. The gel cannot be obtained with any random combination of clay minerals and the ingredients described above. It takes the sequential manufacturing method as follows to get this kind of gel. Firstly, clay minerals and liquid polymers have to be pre-mixed in order to saturate the liquid polymers with the clay minerals. Then the on-processed gel has to be finely crystallized. The clay minerals, which are the core elements for this gel, were used as a function of Binder & Buffer and liquid polymer was mixed together for the deterioration of the surface tension of each component and to form a functional film in the gel. This liquid polymer was combined with clay minerals because it is not miscible with most oils and solvents. Waxes have a function of keeping a solid status in the stick. We reduced the usage of waxes by putting clay minerals as buffer in the proportion of 0.5:1 with oil phase. Ceramide takes care of the skin when used regularly and maintains the skin's moisture. Vitamins A and E contribute to preventing skin aging by the activation of skin cells. We could get the stable viscous gel, which has about 80% oil phase using clay minerals and liquid polymer. The crystalline structures of gel were surface-chemically-analyzed using SEM and Image Analyzer and were thermodynamically analyzed using DSC. Surface tension test and softness were done by Rheometer. In the end, these characteristics were verified by consumer panel tests in Seoul, Daegeon and Pusan in Korea and Hokkaido, Osaka and Miyazaki in Japan with correlation to the climate.

Insecticidal and antifeeding activity of domestic plants against four species of arthropod insect pests

Sang-Gil Lee,Gil-Hah Kim,Il-Kwon Park,Sang-Chul Shin,Ji-Doo Park 한국농약과학회 2000 한국농약과학회 학술발표대회 논문집 Vol.- No.-

Hydroxypheophorbide-α-mediated photodynamic therapy augmented by pretreatment with genistein in<font>CaSki</font>cervical cancer cells

Shin, Jang-In,Ahn, Jin-Chul,Lee, Sang-Joon,Park, Ock Jin,Chung, Phil-Sang World Scientific 2009 Journal of porphyrins and phthalocyanines Vol.13 No.7

<P>Photodynamic therapy (PDT) is a treatment for cancer involving three key components - a sensitizing compound (light) tissue, and oxygen. In this study we applied phototreatment to cancer cells with 2 J.cm<SUP>-2</SUP>of red light after sensitizing with 9-hydroxypheophorbide-α (9-HpbD-α), a new chlorophyll-derived photosensitizer. We have investigated the cytotoxic and apoptotic effects of 9-HpbD-α-induced PDT in cervical cancer cells, the enhancing effect of genistein in PDT, and explored the molecular mechanisms of E6 or E7 oncogenes, apoptotic signaling molecules, and ER stress. Co-treatment downregulated the transcripts of the E6*I, E6*II, and E7 oncogenes. Combined treatment with PDT and genistein showed typical apoptotic features, i.e. apoptotic bodies. To elucidate the mechanism of combination treatment-induced apoptosis, various mediators of apoptosis were investigated. Activation of caspase-8, caspase-3, and PARP were distinct after combination treatment. Furthermore, ER stress-related proteins, such as CHOP and GRP78, were activated after combination treatment. We conclude that genistein sensitizes CaSki cells to apoptosis treated with PDT by 9-HpbD-α (0.59 μg/mL) through mechanisms that involve downregulation of the E6*I, E6*II, and E7 oncogenes, activation of caspase-8 or caspase-3, and ER stress.</P>

Focal localization of MukBEF condensin on the chromosome requires the flexible linker region of MukF

Shin, Ho‐,Chul,Lim, Jae‐,Hong,Woo, Jae‐,Sung,Oh, Byung‐,Ha Blackwell Publishing Ltd 2009 FEBS JOURNAL Vol.276 No.18

<P>Condensin complexes are the key mediators of chromosome condensation. The MukB–MukE–MukF complex is a bacterial condensin, in which the MukB subunit forms a V‐shaped dimeric structure with two ATPase head domains. MukE and MukF together form a tight complex, which binds to the MukB head via the C‐terminal winged‐helix domain (C‐WHD) of MukF. One of the two bound C‐WHDs of MukF is forced to detach from two ATP‐bound, engaged MukB heads, and this detachment reaction depends on the MukF flexible linker preceding the C‐WHD. Whereas MukB is known to focally localize at particular positions in cells by an unknown mechanism, <I>mukE</I>‐ or <I>mukF</I>‐null mutation causes MukB to become dispersed in cells. Here, we report that mutations in MukF causing a defect in the detachment reaction interfere with the focal localization of MukB, and that the dispersed distribution of MukB in cells correlates directly with defects in cell growth and division. The data strongly suggest that the MukB–MukE–MukF condensin forms huge clusters through the ATP‐dependent detachment reaction, and this cluster formation is critical for chromosome condensation by this machinery. We also show that the MukF flexible linker is involved in the dimerization and ATPase activity of the MukB head.</P><P><B>Structured digital abstract</B></P><P><P>: <I>mukBhd</I> (uniprotkb:), <I>mukF</I> (uniprotkb:) and <I>mukE</I> (uniprotkb:) <I>physically interact</I> () by <I>blue native page</I> ()</P></P>

Challenge to Nanospinics

Sung-Chul Shin 한국자기학회 2001 한국자기학회 학술연구발표회 논문개요집 Vol.11 No.2

Effect of Trichloroacetic Acid on the Solubility of Caseinomacropeptide

Sung-Chul Shin,Hae-Dong Jang 한국식품영양과학회 2002 Preventive Nutrition and Food Science Vol.7 No.1

Crude caseinomacropeptide (CMP) was prepared from Na-caseinate using a commercial renneting enzyme. Most of the crude CMP was released from the Na-caseinate by hydrolyzing with the enzyme for 40 min. The hydrolysis of the k-casein with carbohydrate was slower than that of the k-casein without carbohydrate, as shown by the analyses of the sialic acid content, the tricine-SDS-polyacrylamide gel electrophoresis. The yield of crude CMP from Na-caseinate was 3.7%. Cation exchange chromatography showed that the crude CMP consisted of 40.5% CMP, 59.5% caseinogylcomacropetide (CGP). The effect of the TCA concentration on the solubility of CMP, CGP was determined by using crude CMP. The amounts of crude CMP, sialic acid decreased in the proportion to the increase of trichloroacetic acid (TCA) concentration from 2 to 12%, suggesting that the CGP containing carbohydrate, as well as the CMP having no carbohydrate, was precipitated in a range of 4 to 12%, depending on the TCA concentration. This result supports the hypothesis that the different non-glycosylated, glycosylated forms of CMP have different sensitivities to TCA precipitation.

Twin Spray Characteristics Between Two Impinging F-O-O-F Type Injectors

Shin-Jae Kang,Eun-Sang Lee,Ki-Chul Kwon,Je-Ha Oh,Myoung-Jong Yu 대한기계학회 2002 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.16 No.5

This paper presents twin spray characteristics of two impinging F-O-O-F type injectors in which fuel and oxidizer impinge on each other to atomize under the various conditions. The droplet size and velocity in the impinging spray flow field were measured using PDPA. The droplet size and velocity were investigated at the mixture ratios of 1.5, 2.0, 2.47 and 3.0 for four injectors in which two single F-O-O-F injectors were arranged at the intervals of 20.8,31.2,41.6 and 62.4mm respectively. In general. the arithmetic mean diameter, SMD and standard deviation of droplet size in the interaction area (X=0 and Y=0mm) were smaller, while the axial velocity in the interaction area was slightly higher. An empirical correlation is obtained for the (D??) ??/ (D??) c value under the assumptions of two identical droplets and these with different size and velocity. The droplets with low Weber numbers below 40 have possibility to coalesce, while those over 40 tend to disintegrate after impingement in the interaction area.