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Sarabia-Sainz, Hector M.,Ezquerra-Brauer, Josafat Marina,Santacruz-Ortega, Hisila C.,Rouzaud-Sandez, Ofelia,Valenzuela-Soto, Elisa M.,Acosta-Elias, Monica,Torres-Arreola, Wilfrido 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1
Muscle from mantle, fins and arms of squid (Dosidicus gigas) were compared based on lysyl oxidase activity (LOX), chemical/structural and thermodynamic properties of highly cross-linked collagen. The arms collagen presented the highest temperature (Tp) and enthalpy of transition. The arms collagen thermic properties may be explained by the higher imino amino acid content, proline and lysine hydroxylation degrees. Moreover, among the regions, the collagen from the arms had a more intense ${\beta}$ band chain, hydroxymerodesmosine peak in the resonance magnetic nuclear spectra and pyridinoline peak in the Raman spectra. Fins showed the highest LOX activity. The LOX activity was associated with the Tp, proline and lysine hydroxylation degrees. These results implied that the collagen in the arms was more intermolecularly ordered than the mantle and fins, and may provide a theoretical basis for a better understanding of the thermal behaviour of squid tissues during management and processing.
He´ctor M. Sarabia-Sainz,Josafat Marina Ezquerra-Brauer,Hisila C. Santacruz-Ortega,Ofelia Rouzaud-Sa´ndez,Elisa M. Valenzuela-Soto,Monica Acosta-Elias,Wilfrido Torres-Arreola 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.1
Muscle from mantle, fins and arms of squid (Dosidicus gigas) were compared based on lysyl oxidase activity (LOX), chemical/structural and thermodynamic properties of highly cross-linked collagen. The arms collagen presented the highest temperature (Tp) and enthalpy of transition. The arms collagen thermic properties may be explained by the higher imino amino acid content, proline and lysine hydroxylation degrees. Moreover, among the regions, the collagen from the arms had a more intense b band chain, hydroxymerodesmosine peak in the resonance magnetic nuclear spectra and pyridinoline peak in the Raman spectra. Fins showed the highest LOX activity. The LOX activity was associated with the Tp, proline and lysine hydroxylation degrees. These results implied that the collagen in the arms was more intermolecularly ordered than the mantle and fins, and may provide a theoretical basis for a better understanding of the thermal behaviour of squid tissues during management and processing.
Numerical and experimental study of unsteady wind loads on panels of a radar aerial
Scarabino, Ana,Sainz, Mariano Garcia,Bacchi, Federico,Delnero, J. Sebastian,Canchero, Andres Techno-Press 2016 Wind and Structures, An International Journal (WAS Vol.23 No.1
This work experimentally and numerically analyzes the flow configurations and the dynamic wind loads on panels of rectangular L/h 5:1 cross section mounted on a structural frame of rectangular bars of L/h 0.5:1, corresponding to a radar structure. The fluid dynamic interaction between panels and frame wakes imposes dynamic loads on the panels, with particular frequencies and Strouhal numbers, different from those of isolated elements. The numerical scheme is validated by comparison with mean forces and velocity spectra of a panel wake obtained by wind tunnel tests. The flow configuration is analyzed through images of the numerical simulations. For a large number of panels, as in the radar array, their wakes couple in either phase or counter-phase configurations, changing the resultant forces on each panel. Instantaneous normal and tangential force coefficients are reported; their spectra show two distinct peaks, caused by the interaction of the wakes. Finally, a scaled model of a rectangular structure comprised of panels and frame elements is tested in the boundary layer wind tunnel in order to determine the influence of the velocity variation with height and the three-dimensionality of the bulk flow around the structure. Results show that the unsteady aerodynamic loads, being strongly influenced by the vortex shedding of the supporting elements and by the global 3-D geometry of the array, differ considerably on a panel in this array from loads acting on an isolated panel, not only in magnitude, but also in frequency.
Eslava, Carlos,Sainz, Teresita,Perez, Julia,Fresan, Ma.Cristina,Flores, Veronica,Jimenez, Luis,Hernandez, Ulises,Herrera, Ismael The Microbiological Society of Korea 2002 The journal of microbiology Vol.40 No.2
Enteroaggregative E. coil (EAEC) is an important aethiological causal agent of diarrhea in people of developed and undeveloped countries. Different in vitro and in vivo models have been proposed to study the pathdgenic and immune mechanisms of EAEC infaction. The aim of this study was to analyze whether BALB/c mice could be used as an animal model to study EAEC pathogenesis Six-week-old BALB/c mice were inoculated with EAEC strain 042 (044:H88) nalidixic acid resistant, and re-inoc-ulated ten days after. Mice feces were monitored for the presence of the EAEC strain over a period of 20 days . Bacteria were enumerated on MacConkey agar containing 100$\mu$g of nalidixic acid per ml. Results showed that 35% of the animals were colonized for 3 days, 15% for 5 and 10% for more than 7 days . After re-inoculation only 16% of the animals remained colonized for more than 3 days. During the necropsy, the intestinal fluid of same of the infected animals presented mucus and blood. Six of these fluids showed the presence of IgA antibodies againset Pet toxin and IgG natibodies raised against the toxin were also detected in the animal serum. Histopathologic evidence confirms the stimulation of mucus hypersecretion, an increased amount of goblet cells and the presence of bacterial aggregates in the apical surfaces of intestinal epithelial cells. Edema was present in the submucosa. These results suggest that BALB/c mice could be used as an animal model for in vivo study of EAEC infection.
White spots on the mucosal surface of the duodenum in dogs with lymphocytic plasmacytic enteritis
Mercedes García-Sancho,Ángel Sainz,Alejandra Villaescusa,Antonio Rodríguez,Fernando Rodríguez-Franco 대한수의학회 2011 Journal of Veterinary Science Vol.12 No.2
Distended lacteals, described as expanded white villi in duodenum, are strongly indicative of primary intestinal lymphangiectasia. In the present study, we evaluated the significance of white spots present in the duodenal mucosa of dogs with lymphocytic plasmacytic enteritis (LPE). Fifty dogs with LPE were included in this study, and white spots were detected in the duodenal mucosa in 22 dogs during endoscopy. Hypoproteinemia was more frequent in dogs with white spots than in dogs without spots (p = 0.02). Serum protein and albumin concentration were significantly lower in LPE dogs with white spots (p = 0.038) compared to LPE dogs without white spots (p = 0.039). There was a significant correlation between white spots density and lymphatic dilatation histological scores (p = 0.023; ρ = 0.481). These results suggest that the presence of white spots in the duodenal mucosa of dogs is not a finding exclusive for intestinal lymphangiectasia. Low serum protein and albumin concentrations together with lymphatic dilatation seem to be related to the presence of white spots in the duodenal mucosa of LPE dogs.
Differences Between Winning and Losing Teams in Youth Basketball Games (14-16 Years Old)
( Ortega E. ),( Cardenas D. ),( Sainz De Baranda P. ),( Palao J. M. ) 한국스포츠정책과학원(구 한국스포츠개발원) 2006 International Journal of Applied Sports Sciences Vol.18 No.2
Match analysis is common in performance sport to guide training and competition. However, it is not as common at the youth level. Most studies about match analysis have studied the variables of technique and tactics in relation to final actions of a team`s ball possession. However, a few studies have analyzed participation variables of technique and tactics. The purpose of this study was to determine the differences in final and participatory actions of technique and tactics in relation to winning and losing teams, ball possession, and type of attack phase in youth basketball games. The sample consisted of 184 male players from 16 teams with an average age of 15.6 ± 0.2 years. The twenty-four games were analyzed from the men`s under-16 finals of the Championship of Andalusia (Spain). The variables studied were: game result (win or loss), variables related to type of ball possession, and variables related to pattern of play. Two groups of three observers were utilized (one group observed terminal events of each possession and the other observed possession time and pattern of play). The minimum inter-observer correlation was 0.94. The results found significant differences between winning and losing teams in the following variables: winning teams had higher values than losing teams in the following variables: 2-point field goals made, free throws made, dribbling opposed, time of movement, dribble time, use of screens, fast breaks, attack phases from 1-5 seconds, attack phases in which 2 players participated, and attack phases in which 5 players participated. The results showed that the winning teams have patterns of play that use less dribbling, in general, and less dribbling with displacement, in particular; and they used fast attack phases (1-5 s) in which two players participate. Winning patterns of play were those that used either fast breaks or a long attack phase in which all players participated.
Long-Term Evolution of the Electrical Stimulation Levels for Cochlear Implant Patients
Jose Luis Vargas,Manuel Sainz,Cristina Roldan,Isaac Alvarez,Angel de la Torre 대한이비인후과학회 2012 Clinical and Experimental Otorhinolaryngology Vol.5 No.4
imObjectives. The stimulation levels programmed in cochlear implant systems are affected by an evolution since the first switch-on of the processor. This study was designed to evaluate the changes in stimulation levels over time and the relationship between post-implantation physiological changes and with the hearing experience provided by the continuous use of the cochlear implant. Methods. Sixty-two patients, ranging in age from 4 to 68 years at the moment of implantation participated in this study. All subjects were implanted with the 12 channels COMBI 40+ cochlear implant at San Cecilio University Hospital, Granada,Spain. Hearing loss etiology and progression characteristics varied across subjects. Results. The analyzed programming maps show that the stimulation levels suffer a fast evolution during the first weeks after the first switch-on of the processor. Then, the evolution becomes slower and the programming parameters tend to be stable at about 6 months after the first switch-on. The evolution of the stimulation levels implies an increment of the electrical dynamic range, which is increased from 15.4 to 20.7 dB and improves the intensity resolution. A significant increment of the sensitivity to acoustic stimuli is also observed. For some patients, we have also observed transitory changes in the electrode impedances associated to secretory otitis media, which cause important changes in the programming maps. Conclusion. We have studied the long-term evolution of the stimulation levels in cochlear implant patients. Our results show the importance of systematic measurements of the electrode impedances before the revision of the programming map. This report also highlights that the evolution of the programming maps is an important factor to be considered in order to determine an adequate calendar fitting of the cochlear implant processor.
Numerical and experimental study of unsteady wind loads on panels of a radar aerial
Ana Scarabino,Mariano García Sainz,Federico Bacchi,J. Sebastián Delnero,Andrés Cánchero 한국풍공학회 2016 Wind and Structures, An International Journal (WAS Vol.23 No.1
This work experimentally and numerically analyzes the flow configurations and the dynamic wind loads on panels of rectangular L/h 5:1 cross section mounted on a structural frame of rectangular bars of L/h 0.5:1, corresponding to a radar structure. The fluid dynamic interaction between panels and frame wakes imposes dynamic loads on the panels, with particular frequencies and Strouhal numbers, different from those of isolated elements. The numerical scheme is validated by comparison with mean forces and velocity spectra of a panel wake obtained by wind tunnel tests. The flow configuration is analyzed through images of the numerical simulations. For a large number of panels, as in the radar array, their wakes couple in either phase or counter-phase configurations, changing the resultant forces on each panel. Instantaneous normal and tangential force coefficients are reported; their spectra show two distinct peaks, caused by the interaction of the wakes. Finally, a scaled model of a rectangular structure comprised of panels and frame elements is tested in the boundary layer wind tunnel in order to determine the influence of the velocity variation with height and the three-dimensionality of the bulk flow around the structure. Results show that the unsteady aerodynamic loads, being strongly influenced by the vortex shedding of the supporting elements and by the global 3-D geometry of the array, differ considerably on a panel in this array from loads acting on an isolated panel, not only in magnitude, but also in frequency.