산소 장력이 치주인대에서 유래한 세포의 활성과 기능에 미치는 영향

사명희,양원식 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.3

정치료를 위해 발거한 제 1 소구치에서 사람의 치주인대 세포를 채취하여 37℃, 5% CO₂, 100% 습도의 환경에서 배양한 후 5 내지 6 계대배양하여 실험에 사용하였다. Gaspack system에 0.2㎛ Millipore filter를 부착하여 세균 감염을 막았고, 저산소군에는 10% O₂, 5% CO₂, 85% N2 가스를, 과산소군에는 90% O₂, 5% CO₂, 5% N₂ 가스를 연결하여 산소 장력에 변화를 주었으며 대조군에는 5% CO₂, 95% 공기를 공급하였다. 37℃에서 2, 4, 6일간 배양한 후에 세포활성을 측정하기 위해 tetrazolium(MTT) assay를 시행하였고 sulforhodamine B(SRB) assay를 이용하여 총 단백질 합성을 알아보았으며, 4-hydroxyproline 측정으로 교원질 합성을 측정해 보았다. 또한 효소면역측정법으로 IL-1β, Il-6, TNF-α를 측정하여 다음과 같은 결과를 얻었다. 1. 저산소군에서 세포 활성과 총 단백질 합성은 대조군보다 다소 높거나 같은 정도이었다. 2. 과산소군에서 세포 활성은 대조군보다 낮았으며 총 단백질 합성도 다소 감소되었다. 3. 교원질 합성은 저산소군과 과산소군에서 대조군보다 유의하게 감소되었다가 배양기간이 길어짐에 따라 증가되어 대조군과 유사한 정도에 달했다. 4. 효소면역법 측정 결과 IL-6, TNF-α, IL-1β순으로 다량 검출되었다. 5. IL-6, TNF-α, IL-1β는 저산소군과 과산소군에서 배양기간이 길어짐에 따라 대조군에 비해 급격히 증가하였다. 6. IL-6, TNF-α는 과산소 조건에서 배양6일 후에는 대조군에 비해 유의하게 증가하였으며 이는 배양 2일 또는 4일 후보다도 유의하게 증가한 것이었다. This study was undertaken to investigate the effect of oxygen tension on the activity and function of the cells derived from human periodontal ligament by measuring cell activity, total protein synthesis, collagen synthesis, IL-1β, IL-6, TNF-α. Human periodontal ligament fibroblasts were collected from premolars extracted for orthodontic treatment and incubated in the environment of 37℃, 5% CO₂, 100% humidity. After the fifth to sixth passage they were used for the experiment. Gaspack system to which 0.2㎛ Millipore filter was attached was connected to mixed-gas tanks. The mixed gases were composed of 10% O₂, 5% CO₂, 85% N₂ in hypoxic group of 90% O₂, 5% CO₂, 5% N₂ in hyperoxic group and 5% CO₂, 95% air for control. After incubation in 37℃ for 2, 4, 6 days, cell activity was determined by tetrazolium(MTT) assay and total protein synthesis was assayed using sulforhodamine B(SRB). And measurement of 4-hydroxyproline was performed to assess collagen synthesis and IL-1β, IL-6 and TNF-α were measured by enzymeimmunoassay. The results were as follows. 1. The cell activity and total protein synthesis in hypoxic group were a little higher than or almost the same with those in control group. 2. In hyperoxic group, the cell activity was lower than that in control group and total protein synthesis was decreased. 3. Collagen synthesis was significantly decreased initially in both hypoxic and hyperoxic group and increased nearly to the level of control group as the duration of cell incubation was longer. 4. As a result of enzymeimmunoassay, the amount of cytokines was IL-6, TNF-α and IL-1β in order. 5. IL-6, TNF-α and IL-1β were increased more rapidly in both hypoxic and hyperoxic group than in control group as the duration of cell incubation was longer. 6. There were more Il-6 and TNF-α in hyperoxic group than in control group after 6 days, and there were more Il-6 and TNF-α after 6 days than after 2 or 4 days in hyperoxic group. These results suggested that oxygen tension might modulate the production of extracellular matrix and cytokines in the cells derived from human periodontal ligament.

홍삼 엑기스를 함유한 분말주의 제조 및 평가

이사원,최한곤,박정일,김종국 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-

To develop a dry alcohol containing red ginseng extract, dry alcohols composed of ethanol, water, dextrin and sodium lauryl sulfate were prepared using spray dryer, and their ethanol contents and encapsulation efficiencies were deter-mined. An optimal dry alcohol containing red ginseng extract was chosen and the feeling for its oral administration was eval-uated. Dextrin at dextrin/water weight ratios below 1.6/1 and ethanol at ethanol/water weight ratios below 1/1 remarkably increased both the ethanol contents and encapsulation efficiencies of dry alcohols. However, dextrin at dextrin/ water weight ratios above 1.6/1 and ethanol at ethanol/water weight ratio above 1/1 slightly decreased the both parameters. It might be due to the low solubility of dextrin in ethanol and limited diffusion coefficient of ethanol to the dextrin shell. Furthermore, 0.5%(w/w) sodium lauryl sulfate gave the maximum ethanol content of dry alcohol. The more increased amounts of red ginseng extract were added, the more increased amounts of ginsenoside Rb1 but the more decreased amounts of ethanol were encap-sulated in dry alcohols. A dry alcohol containing red ginseng extract was prepared with dextrin/ethanol/water(1/1/1, w/w/w) mixed solution, in which 0.5%(w/w) sodium lauryl sulfate and 20%(w/w) red ginseng extract were dissolved. It contained the ethanol contents of 31.17±1.33%(w/w) and ginsengoside Rb1 of 243.0±7.0㎍/g. It gave the moderate taste of red ginseng extract at its oral administration with or without water. Thus, the dry alcohol containing red ginseng extract can be further developed as a more convenient dosage form for red ginseng extract.

한국인의 아포지단백 E 유전형과 혈청 지질 성분과의 관련성

전사일,김종원,송정한,김진규 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.5

서울 남산 화강암의 풍화에 관한 환경광물학적 연구

김원사,이대준,신현숙 忠南大學校 環境問題硏究所 1998 環境硏究 Vol.16 No.-

The weathering of Namsan granite of Seoul was studied by polarizing microscopy, X-ray fluoresence spectroscopy and X-ray diffraction. The rock consists principally of quartz, feldspars and micas, together with very small amount of garnet, sillimanite, chlorite, sericite, muscovite, cordierite, zircon, and opaque minerals. Under the polarizing microscope, the weathering of the rock begins to breakdown of the feldspars into sericite and is then followed by alteration of quartz into micas. Decrease of amount of feldspars and quartz and increase of micas, with progress of weathering, are also confirmed by X-ray diffractogram. With advancing weathering, K₂O and Al₂O₃ markedly increased, while Na₂O, CaO, Fe₂O₃, TiO₂, and MnO₂ decreased. When water having penetrated into the rock through cracks and pore space developed between the minerals it becomes to react with minerals in contact resulting in strong alkaline or weak acidic solution. This kind of solution is interpreted to be a principal factor for the wathering of the rock.

텍타이트와 흑요석의 특징 및 성인 비교 : Their Characteristics and Origin

김원사,신현숙 충남대학교 기초과학연구소 2003 忠南科學硏究誌 Vol.30 No.1

During the past fifty years, the origin of natural glassy objects, called tektite, has long been a topic in the science community. In this study mineralogical and chemical data for tektites, collected from China, have been investigated. Obsidian, a very similar resemblance, has also been studied, in the hope to characterize it better and also to compare it with tektites. It is confirmed, on the basis of presently obtained data, that the Chinese teckites are believed to originate from terrestrial rocks melted by hypervelocity impacts of large and extraterrestrial objects. In contrast, obsidian is a volcanic glass of rhyolitic composition and is originated from magma during volcanic eruption.

한반도 중서부 지각구조와 물성연구 : 부여록옥광산에서 산출되는 귀사문석의 광물학적 특성 Mineralogy of precious serpentine from the Booyo Green Jade Mine

김원사,정지곤,이병임,이재열 충남대학교 기초과학연구소 1995 연구논문집 Vol.15 No.-

충남 부여군 외산면에 위치한 부여록옥광산은 원래 석면을 대상으로 가행되었던 광산이었다가 폐광된 후 최근 세계적으로도 매우 희귀한 보석으로 인정받고 있는 귀사문석이 산출되고 있음이 발견되어 현재 채광작업을 재개하고 있다. 부여산 귀사문석은 광산의 주변암체인 선캠브리아기의 편암을 관입압 사문암체를 후기에 이를 다시 관입한 그로슐라 석류석맥의 접촉부를 따라 발달하고 있다. 이번 연구에서는 부여산 귀사문석에 대한 광물학적, 보석학적 특성을 규명하기 위하여 편광현미경, 비중계, 굴절계, 경도계, X선회절분석기, 화학분석, 적외선분광분석법 등을 이용해 연구하였다. 부여산 귀사문석은 짙은 녹색을 띠고 아투명하며 높은 지방광택을 나타난다. 모스경도는 H=5∼6이며 인성은 매우 높은 편이다. 비중은 2.67, 스포트법으로 측정한 굴절율은 No=1.56이다. X선회절분말양상은 7.40(100), 4.64(25), 3,68(68), 2.757(69), 2.530(49), 2.459(32), 2.113(32), 1.710(21)으로 안티고라이트임을 알 수 있었으며, 모암인 사문암도 안티고라이트로 구성되어 있다. 그러나 귀사문석과 사문암의 투명도와 경도 등이 다른것은 구성광물결정의 조직차이가 큰 요인인 것으로 여겨진다. 귀사문석의 주요 화학성분은 42.49% SiO_2, 39.08% MgO, 3.85% Fe_2O_3, and 11.87% H_2O 등이며, 미량원소로는 Cr(2188 ppm), Ni(1110ppm), Co(58 ppm), and Ta(108 ppm) 등이 있다. 적외선분광분석결과는 3600, 1190, 1070, 980, and 610 cm^-1에 흡수 peak를 나타내었다. 부여산 귀사문석(안티고라이트)은 감람석과 휘석으로 구성된 초염기성암이 변질되어 형성된 것으로 해석되며 그 생성반응은 (Fe, Mg)_2SiO_4 (감람석)+(Mg, Fe)SiO_3 (휘석)+2H_O→M_3sI_2O_5(OH_4)(티고라이트)+Fe_3O_4 (자철석). The Booyo Green Jade mine, located in Oesan-myon, Booyo-gun, Chungnam-do, had originally been developed for asbestos. Recently it has re-opened after discovery of precious serpentine. The precious serpentine is a gem variety of serpentine and is regarded as a rare and valuable gem material in the world. The precious serpentine develops along the contact between grossular garnet vein within the host rock serpentinite that had intruded schist of Precambrian age in the area. In this study mineralogical and gemmologicl properties of the precious serpentine from Booyo have been investigated by using polarizing microscope, specific gravity balance, refractometer, hardness tester, X-ray diffractometer, XRF and ICP-MS analyers, and infrared absorption spectroscope. The Booyo precious serpentine is deep green in color an semi-transparent, and also yields a highly oily luster. Mohs's hardness is measured to be H=5-4 with excellent toughness. Specific gravity is determined to be 2.67, and refractive index N_D=1.56 by a distant-vision method. Xray diffraction data shows peaks of 7.40(100), 4.64(25), 3,68(68), 2.757(69), 2.530(49), 2.459(32), 2.113(32), 1.710(21), which corresponds well to that of antigorite. The higher transparency and hardness of the precious serpentine than the neighboring serpentinite are most probably due to textural arrangement of constituent antigorite crystals. The major chemical constituents of precious serpentine are 42.49% SiO_2, 39.08% MgO, 3.85% Fe_2O_3, and 11.87% H_2O with trace elements Cr(2188 ppm), Ni(1110ppm), Co(58 ppm), and Ta(108 ppm). Infrared absorption spectroscopic analysis shows peaks at 3600, 1190, 1070, 980, and 610 cm^-1, Formation of the precious serpentine(antigorite) from ultramafic rock, which was made up of olivine and pyroxene, may represented by the following reaction: (Fe, Mg)_2SiO_4 (olivine)+(Mg, Fe)SiO_3 (pyroxene)+2H_O→M_3sI_2O_5(OH_4)(antigorite)+Fe_3O_4 (magnetite).

제주 우수 육상경기 선수들의 년중 훈련효과 분석 및 처방

김철원,임상용,김승곤,오만원,김성찬,이창준,남사웅,이세형,류재청 濟州大學敎 體育科學硏究所 1996 체육과학연구 Vol.2 No.-

The study was undertaken to analysis, evaluate, and prescribe t h e annual effect of training of periodically anaerobic and aerobic motor ability for Che-Ju representive team of elementary, middle and high school athletes. first. the performance can be obtained when it was prescribed proper training after analysis of physical fitness by each events. second, individually fitness. scientific analysis. prescription. proper management of athlete will be necessary to apply. In addition to physiology aspect, it is essential to consider sport biomechanics. sport psychology when we prescribe specific events and necessary approach in demension coach-athlete-researcher for individual.

중추신경계 낭미충증에 관한 임상적 고찰

정원영,최일생 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.2

We reviewed clinical features of 133 cases with neurocysticercosis who admitted at Severance Hopital Yongdong Severance Hospital and Chosun University Hospital from March, 1985 to February, 1991 The clinical results were summarized as follows; 1. The ages ranged from 10 to 76 years(mean age 50.0 years)and most of them were 5th or 6th decades 2. Among 133 cases of neurocysticercosis, Parenchymal type was 83 cases (62.4%), mixed type was 25cases(18.8%), ventriculomeningeal type was 23 cases (17.3%) and spinal type was 2 cases (1.5%). 3. The most two frequent initial symptoms were seizure (53.4%) and headache(24.1%). And frequent neurologic symptoms were seizures(64.7%), headache(61.7%), focal neurologic deficit(30.8%), mental changes (13.5%), visual disturbances (12.0%), vertigo(9.8%), cranial nerve pa1sy(6.8%), behavioral symptoms(3.0%), and urinary incontinence (2.3%) in order. 4. There were statistically significant differences in CSF 1eucocytosis(P <0.01), CSF / serum glucose ratio(P < 0.05) between different types. But there were no differences in CSF pressure, protein and glucose level, and ELISA titer. 5. 108cases(81.2%) who received medical treatment only had good prognosis. The overall mortality was 3.7% (5 cases) and causes of death were postoperative infection and shunt malfunction, all occured in ventnculo-meningeal type.

초고집적소자의 층간절연막용 polysilazane계 spin on glass (SOG)에 관한 연구

나사균,정석철,이재관,김진우,홍정의,이원준 대전산업대학교 반도체기술연구소 2000 半導體技術硏究所報 Vol.2 No.-

초고집적소자의 층간절연막으로서의 polysilazane계 spin on glass (SOG)의 적용 가능성을 평가하였다. 상용화된 무기 SOG 계열의 polysilazane과 polysilazane에 산화제를 첨가하여 SiO_2로의 변환 능력을 향상시킨 물질을 spin coating하고 curing하여 SOG 막을 제조한 다음, 400℃에서 900℃까지의 온도범위에서 열에 SOG막을 형성하여 gapfill 특성을 비교하였으며, SOG막에 contact hole을 형성한 다음 습식 세정에 대한 저항성을 평가하였다. 산화제를 첨가함으로써 polysilazane polymer 구조에서 SiO_2로의 변환이 더욱 효과적으로 이루어져, 기존 SOG 막에 비해 밀도 및 습식세정 chemical에 대한 저항성이 우수하고거 gapfill 특성은 동등한 수준인 SOG 막을 제조할 수 있었다. 특히, 기존의 SOG 막의 경우 좁은 gap space 내에서 curing이 충분하게 이어나지 않아 습식 세정시 hole profile이 나빠지는 문제를 산화제 첨가를 통해 해결할 수 있었다. We have investigated the feasibility of spin on glass (SOG) film from polysilazane-type resin as a premetal dielectric (PMD) layer of the next-generation ultra-large scale integrated (ULSI) devices. A commercial polysilazane resin and a polysilazane-type resin with oxidzing agent were spin-coated and cured to form SOG films. In order to study the effect of oxidizing agent and annealing, the SOG films were characterized as cured and after annealing at 400℃ to 900℃. The density and the resistance against wet chemical of the SOG films were improved by the addition of oxidizing agent, because oxidizing agent enhanced the conversion from polysilazane polymer to siO_2. The hole profile issue associated with insufficient curing of polysilazane innarrow gaps was alao resolved by oxidizing agent, while the gapfill capability of SOG was not deteriorated by oxidizing agent.

청소년의 가정환경과 도덕발달단계가 행동장애에 미치는 영향

진태원,이흥표,김사준,조수철 대한소아ㆍ청소년정신의학회 1997 소아청소년정신의학 Vol.8 No.2