Osseointegration을 이용한 절단된 수지 재건의 임상치험례

방사익,박양수,문구현 충북대학교 의과대학 충북대학교 의학연구소 1998 忠北醫大學術誌 Vol.8 No.2

연구목적 : 절단된 무지의 재건시 족지 유리전이술,무지형성술등이 시행되어왔으나 이는 숙련된 기술을 요하고 공여부의 희생이 문제되어왔다. 보다 기술적으로 용이하고 간편하며 쉽게 적용할 수 있는 수술법을 찾고자 골통합술을 무지결손환자에게 시도하였다. 재료 및 방법 : 수지절간 관절부위의 외상성 무지절단환자에서 골통합 티타늄 고정기을 이용하여 무지보형물을 무지근위지골에 접합시키려 2단계 수술을 시행하였다. 1단계로 장골 해면 질골이식과 같이 고정기를 근위지골 원위단 골수강래에 삽입시켰다. 고정기가 주변골과 통합이 되는 3개월째에 고정기를 노출시키고 그 위에 접합체를 고정시키는 수술을 실시하였다. 2주후 무지보형물을 접합체를 이용 고정기에 고정시킬수 있었다. 결과 : 술후 12개월간 추적관찰한 결과 고정기의 골통합이 유지되었고, 피부괴사 등의 합병증은 발생하지 않았으며 어느정도의 식별촉각을 보였다. 결론 : 절단된 수지의 재건에 있어서 골통합을 이용한 보형물법이 선택적 경우에 효과적인 대안이 될 수 있겠다 Purpose : Reconstruction of amputated finger is challenging field. Free toe-to-finger transfer, pollicization are well-known useful methods but require skillful techies, sacrifice of donor digit. To find and evaluate more readily appliable method, we tried to reconstruct amputated finger using osseointegration. Materials and Methods : A case with traumatic amputation of the thumb at the interphalangeal joint level underwent two stage reconstruction aimed at fixation of a thumb prosthesis to the first proximal phalangeal bone via an osseointegrated titanium fixture. The first stage included insertion of the fixture into the medullary cavity of the first proximal phalanx in combination with transplantation of cancellous bone from the iliac crest. After 3 months. when the fixture was firmly integrated into the bone, a skin-penetrating abutment was placed on top of the fixture, the surrounding skin being thinned to the thickness of a split-thickness skin graft to minimize relative mobility. A thumb prosthesis could then be firmly attached to this fixture. Results : At follow-up examination at 12 months postoperatively, good osseointegration of the implant persisted. There was no skin problems. Some extent of tactile discrimination was achieved in the prosthesis hypothetically based on transfer of tactile stimuli to endosteal nerves in the bone via the titanium fixture. Conclusion : Amputated finger can be successfully reconstructed using osseointegrated implant without sacrifice of donor toe or finger.

좌골부 및 전자부 욕창의 재건을 위한 후대퇴근막 피판술

문구현,방사익 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.2

연구목적: 욕창은 장기적으로 반복되는 압박으로 인해 피부 및 연부조직에 생긴 괴사를 말하며 이는 주로 골 돌출부 주위에 잘 발생한다. 이러한 욕창은 교통사고 및 산업재해의 증가로 척추손상 및 지체마비 환자가 늘어나고 집중 치료를 요하는 의식불명, 만성병증의 환자수가 증가하면서 그 발생 빈도도 증가 하고 있다. 그리고 욕창은 천골부, 대전자부 및 좌골부 등에서 호발한다. 추후에 보행이 가능한 환자의 경우 근피판보다는 근육을 보존할 수 있는 근막피판을 사용하는 것이 수술 후 보행에 더 도움을 줄 수가 있다. 그러므로 기존에 수술적 치료를 시행한 환자의 경우나, 욕창의 크기가 큰 경우, 추후에 보행이 가능한 환자에게 있어서 사용할 수 있는 술식의 유용성을 확인하고자 했다. 대상 및 방법 : 1999년 9월부터 2000년 8월까지 본원에 입원 중이던 6 례의 전자부 욕창과 5례의 좌골부 욕창을 가진 남자환자 7명, 여자환자 4명을 대상으로 하였으며, 원인별로 볼 때는 하반신 마비가 가장 많았다. 하둔부동맥 및 대퇴 심동맥의 제 1, 2 심부관통동맥으로부터 혈액공급을 받는 후대퇴근막피판을 작성한 후 피판을 전위하는 방법을 사용하였다. 결과: 피부 괴사등의 합병증 없이 모른 예에서 결손 부위을 막을 수가 있었으며 적당한 연부조직을 함께 전위 시켜줌으로 해서 추적관찰 기간 동안 욕창의 재발을 방지 할 수 있었으며 보행이 가능한 환자에 있어서도 보행에 문제가 없었다. 결론: 하둔부동맥 및 대퇴 심동맥의 제 1, 2 심부관통동맥으로부터 혈액 공급을 받는 후대퇴근막피판술은 수술이 쉽고, 출혈이 비교적 적으며 수술시간이 짧고, 피판 공여부의 봉합이 쉬우며, 보행 가능한 환자에서는 하지 근육의 기능을 보존할 수 있고, 만약 욕창이 재발 시 피판을 재거상 하여 전진시킴으로서 치료가 가능하며, 근육의 손상을 주지 않으므로 재발시 다른 수술 적 방법을 고려할 수 있는 여러가지 장점이 있는 술식이라 판단된다. Purpose : Pressure sore is an area of ischemic tissue loss resulting from prolonged or often repeated pressure, usually over a bony prominence of body. There has been recent reports describing superior durability of fasciocutaneous flap in sore reconstruction over musculocutaneous flap. Contrary to common musculocutaneous flap using gluteus & harmstring muscle, posterior fasciocutaneous flap does not incorporate any muscle, making it useful especially in ambulatory patients. Material and Methods : From September 1999 to October 2000 we have used posterior thigh fasciocutaneous flap for coverage of six trochanteric sores and five ischial sores. Posterior thigh fasciocutaneous flap is perfused by first & second profunda femoris perforating arteries. Results: All the patients recovered without any serious complications. Three cases developed minimal hematoma but it was corrected by simple evacuation. During the follow up period none of the patients were recurred and for those patients who were able to walk, there was no problem in walking. Conclusion : Posterior thigh fasciocutaneous flap has several advantages. It is easier to elevate, less operation time, less hematoma formation, good donor site morbidity and making possible to preserve the muscle component.

NGS 데이터를 기반으로 한 PGPR의 기능에 대한 분자생물학적 접근

황보경, 박상익, 김기윤, 사동민, 이이 忠北大學校 農業科學硏究所 2017 農業科學硏究 Vol.33 No.1

There are numerous nutrients, including essential elements, in the soil. Nevertheless, most of the nutrients that exist in the soil are not suitable for the growth of plants. Therefore, in agriculture, chemical fertilizers have been widely used for the increasing of crop production from long time ago. As a result, soil nutrient has been over accumulated, environment was polluted and pathogens gained resistance. As a solution about these problems, plant growth promoting rhizobacteria (PGPR) could be used for the promotion of plant growth instead of chemicals. Recently, numerous studies on microbial plant growth promoting (PGP) characters were conducted based on molecular biology like genome sequencing by next generation sequencing (NGS) technique. In this review, we discuss PGP characters about nitrogen fixation, solubilizing/mineralizing phosphorus, production of siderophore, degradation of ethylene and biosynthesis of IAA and cytokinin at gene level and predict the essential set of genes for the PGPR function.

오른손잡이 숫흰쥐의 해마복합체에서 VIP 신경세포의 비대칭적 분포에 관한 면역세포화학적 연구

손성기,이영일,이병란,조자선,백상호,차중익 대한해부학회 1995 Anatomy & Cell Biology Vol.28 No.1

Quinpirole Increases Melatonin-Augmented Pentobarbital Sleep via Cortical ERK, p38 MAPK, and PKC in Mice

( Sa Ik Hong ),( Seung Hwan Kwon ),( Ji Young Hwang ),( Shi Xun Ma ),( Jee Yeon Seo ),( Yong Hyun Ko ),( Hyoung Chun Kim ),( Seok Yong Lee ),( Choon Gon Jang ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Sleep, which is an essential part of human life, is modulated by neurotransmitter systems, including gamma-aminobutyric acid (GABA) and dopamine signaling. However, the mechanisms that initiate and maintain sleep remain obscure. In this study, we investigated the relationship between melatonin (MT) and dopamine D2-like receptor signaling in pentobarbital-induced sleep and the intracellular mechanisms of sleep maintenance in the cerebral cortex. In mice, pentobarbital-induced sleep was augmented by intraperitoneal administration of 30 mg/kg MT. To investigate the relationship between MT and D2-like receptors, we administered quinpirole, a D2-like receptor agonist, to MT- and pentobarbital-treated mice. Quinpirole (1 mg/kg, i.p.) increased the duration of MT-augmented sleep in mice. In addition, locomotor activity analysis showed that neither MT nor quinpirole produced sedative effects when administered alone. In order to understand the mechanisms underlying quinpirole-augmented sleep, we measured protein levels of mitogen-activated protein kinases (MAPKs) and cortical protein kinases related to MT signaling. Treatment with quinpirole or MT activated extracellular-signal-regulated kinase 1 and 2 (ERK1/2), p38 MAPK, and protein kinase C (PKC) in the cerebral cortex, while protein kinase A (PKA) activation was not altered significantly. Taken together, our results show that quinpirole increases the duration of MT-augmented sleep through ERK1/2, p38 MAPK, and PKC signaling. These findings suggest that modulation of D2-like receptors might enhance the effect of MT on sleep.

Anxiolytic-Like Effects of Chrysanthemum indicum Aqueous Extract in Mice: Possible Involvement of GABAA Receptors and 5-HT1A Receptors

( Sa Ik Hong ),( Seung Hwan Kwon ),( Min Jung Kim ),( Shi Xun Ma ),( Je Won Kwon ),( Seung Min Choi ),( Soo Im Choi ),( Sun Yeou Kim ),( Seok Yong Lee ),( Choon Gon Jang ) 한국응용약물학회 2012 Biomolecules & Therapeutics(구 응용약물학회지) Vol.20 No.4

Chrysanthemum indicum Linne is an ancient herbal medicine used to treat bone and muscle deterioration, ocular infl ammation, headache, and anxiety in Korea, China, and Japan. Furthermore, tea derived from Chrysanthemum indicum Linne has been used to treat anxiety by facilitating relaxation and curing insomnia. However, no reports exist on the anxiolytic-like effects of Chrysanthemum indicum Linne water extract (CWE) in mice. In the present study, we investigated the anxiolytic-like effects of CWE using the elevated plus-maze (EPM) test in mice. CWE, at a dose of 500 mg/kg (p.o.), signifi cantly increased the time spent in the open arms of the EPM compared to a vehicle-injected control group. Moreover, the effect of CWE (500 mg/kg) was blocked by bicuculline (a selective GABAA receptor antagonist) and WAY 100635 (a selective 5-HT1A receptor antagonist). Taken together, these fi ndings suggest that the anxiolytic-like effects of CWE might be mediated by the GABAA receptor and the 5-HT1A receptor.

대심도 연약지반에 설치된 라멘 구조물의 시공 및 보강사례

이사익(Sa-Ik Lee),최영철(Young-Chul Choi),유상호(Sang-Ho Yoo),김태형(Tae-Hyung Kim),김성렬(Sung-Ryul Kim) 한국지반신소재학회 2014 한국지반신소재학회 논문집 Vol.13 No.1

연약지반에 설치되는 구조물은 장기간에 걸쳐 발생하는 연약지반의 압밀 침하 또는 측방유동 현상에 의하여 많은 문제점들이 발생할 수 있다. 그러므로, 지반조사, 지반개량, 시공관리 등과 관련하여 적합한 설계 및 시공순서를 결정하는 것이 중요하다. 본 연구의 시공사례는 기초구조물로 지지되는 라멘구조물을 대상으로 하였다. 특히, 본 사례는 공사기간의 제약과 새로운 고속도로를 가로지르는 기존 도로의 연결 문제 등으로 구조물 하부 연약지반을 개량하지 않고 배면 성토를 시공하기 전에 구조물을 먼저 시공하였다. 이에 따라, 구조물의 안정성을 확보하기 위하여 수치해석 및 지반계측 결과를 바탕으로 시공절차 및 대책공법을 주의깊게 계획하였다. 시공과정 중에 여러 번의 시행착오를 거쳐 성공적으로 구조물 시공을 완료할 수 있었다. Structures that have constructed in soft clay might suffer from many issues related to consolidation settlement or lateral movement of soft-clay during long-term period. Therefore, it is important to establish proper design and construction processes related to site investigation, soil improvement, construction management, and so on. This case study focused on the construction of the rahmen structure supported by pile foundations. Especially, the structure in this case had been constructed without improving underlying soft clay and before constructing backfill embankment due to the limited construction time and the traffic connection of the old road crossing new highway. Therefore, in order to satisfy the structural stability, the construction processes and countermeasure methods were carefully planned based on the results of preliminary numerical analyses and monitoring of ground behaviors. Through the trial and error precess during the construction, the structures had been successfully constructed.

Quinpirole Increases Melatonin-Augmented Pentobarbital Sleep via Cortical ERK, p38 MAPK, and PKC in Mice

Hong, Sa-Ik,Kwon, Seung-Hwan,Hwang, Ji-Young,Ma, Shi-Xun,Seo, Jee-Yeon,Ko, Yong-Hyun,Kim, Hyoung-Chun,Lee, Seok-Yong,Jang, Choon-Gon The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.2

Sleep, which is an essential part of human life, is modulated by neurotransmitter systems, including gamma-aminobutyric acid (GABA) and dopamine signaling. However, the mechanisms that initiate and maintain sleep remain obscure. In this study, we investigated the relationship between melatonin (MT) and dopamine D2-like receptor signaling in pentobarbital-induced sleep and the intracellular mechanisms of sleep maintenance in the cerebral cortex. In mice, pentobarbital-induced sleep was augmented by intraperitoneal administration of 30 mg/kg MT. To investigate the relationship between MT and D2-like receptors, we administered quinpirole, a D2-like receptor agonist, to MT- and pentobarbital-treated mice. Quinpirole (1 mg/kg, i.p.) increased the duration of MT-augmented sleep in mice. In addition, locomotor activity analysis showed that neither MT nor quinpirole produced sedative effects when administered alone. In order to understand the mechanisms underlying quinpirole-augmented sleep, we measured protein levels of mitogen-activated protein kinases (MAPKs) and cortical protein kinases related to MT signaling. Treatment with quinpirole or MT activated extracellular-signal-regulated kinase 1 and 2 (ERK1/2), p38 MAPK, and protein kinase C (PKC) in the cerebral cortex, while protein kinase A (PKA) activation was not altered significantly. Taken together, our results show that quinpirole increases the duration of MT-augmented sleep through ERK1/2, p38 MAPK, and PKC signaling. These findings suggest that modulation of D2-like receptors might enhance the effect of MT on sleep.

The effects of simulated acid rain on the seeds germination and seedlings growth of Quercus acutissima and Pinus rigida × P.taeda

Kim, Sa Ik,Kim, Chang Ho 동국대학교 생명자원과학대학 연습림 1989 演習林硏究報告 Vol.- No.1

人工酸性雨가 상수리나무(Quercus acutissima)와 리기테다 소나무(Pinus rigida×P. taeda) 種子의 發芽 및 生長에 미치는 影響을 調査하였다. 人工酸性雨는 黃酸과 室酸을 3 : 2 濃度 混合하여 地下水로 稀釋한 pH2.0, pH3.0, pH4.0, pH5.0 調製하였으며 pH6.4는 對照用으로 使用하여 天然降水를 遮斷한 가운데 상수리나무와 리기테다소나무 種子와 幼苗의 生長期間동안 관수하였다. 種子의 發芽, 樹高, 葉數, 葉??, 葉長, 葉被善率, 根元莖, 生重量, 乾重量 T/R 系, 土壞의 物理化學的 特性을 測定하였으며 그 結果는 다음과 같다. 1. 상수리나무 種子의 發芽는 pH2.0 처리구에서 發芽率 64%로 가장 높게 나타나 1%의 有意水準에서 有意性이 인정된 반면, 리키테다소나무는 pH2.0 처리구에서 가장 낮아 處理間 有意差가 인정되었다. 2. 상수리나무의 樹高長生은 pH5.0 처리구에서 가장 높았고 리키테다 소나무는 pH4.0 處理區에서 가장 높게 나타났다. 3. 상수리나무의 엽수는 처리별 큰 差異가 없었지만 葉長 및 葉幅은 pH2.0 처리구에서 5%有意水準에서 有意性이 인정되었다. 4. 상수리나무의 엽피해율은 處理酸性雨의 pH 값이 낮아질수록 被害率과 被害面種이 증가하였다. 5. 處理酸性雨의 pH 값이 낮아짐에 따라 두 樹種 모두 上壞의 pH 값은 점차적으로 감소하였다. 6. 上壞의 全窒表合量은 소나무에서 처리간 差異가 있었으며 pH4.0 처리구에서 0.180으로 가장 높았다. Quercus acutissima and Pinus rigida × P. taeda seeds were irradiated with simulated acid rains to estimate the germination and growth of those two species and the physical and chemical properties of the experimental soils. Simulated acid rains (pH2.0 pH3.0 pH4.0 and pH5.0) were prepared by diluting sulfuric and nitric acid(H_2SO_4: HNO_3= 3:2v/v) with tap water were treated twice a week. The results obtained in this study were as follows: 1. Germination of Quercus acutissima seeds showed the highest value by 64% at pH2.0 treatment and were significantly different among treatment at 1% level. But germination of P. rigida ×P. taeda seeds was lowest pH2.0 treatment. 2. Height growth of Quercus acutissima and Pinus rigida × P. taeda seedings were the highest at pH5.0 and pH4.0 treatment respectively 3. Leaf number of Quercus acutissima seedings were insignificantly different among treatment and leaf length and leaf width were significantly different among treatment at 5% level. 4. Leaf injury (injury leaf rate and injured leaf area) increased with decreaing pH levels of simulated acid rain. 5. Root collar diameter of Q. acutissima and Pinus rigida × P. taeda seedings were the highest at pH6.4 treatment and decreased with decreasing pH levels gradually. 6. Fresh weight of Q. acutissima seedings at pH4.0 treatment was the highest and that of P. rigida ×P. taeda seedings was the highest by 1.63g at pH3.0 treatment. 7. According to decreasing pH level of simulated acid rain, pH values of soil in two species were gradually decreased.

Region-specific changes of CAMKⅡ in cannabinoid receptor type 1 knockout mice

HONG Sa-Ik,YOU In-Jee,KWON Seung-Hwan,JUNG Yang-Hee,KIM Min-Jung,MA Shi-Xun,JOO Hyun-Joong,HWANG Ji-Young,OH Ji-Hwan,KIM Hyoung-Chun,LEE Seok-Yong,JANG Choon-Gon 大韓藥學會 2012 大韓藥學會 總會 및 學術大會 Vol.2012 No.1