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        • KCI등재SCOPUS


          Shao,,Shao,,Wen-Zhu,An,,An,,Xi-Yong 한국재료학회 1995 Fabrication and Characterization of Advanced Mater Vol.2 No.4

          Blanks of SiCp/Al-Li composite were produced by way of powder explossion consolidation method which has been optimized in parameter thriugh expoeriment. By SEM, it was found that the microstructure of this composite has following characteristics : (1) SiC particles are uniformlu distributed in the composite ; (2) Al-Li powders still remain original dendrite structure ; (3) There is some metalluryical bonding between composite particles ; (4) hine particles deform or milt preferentiallu and along some direction deformation of Al-Li particles ; (5) SiCp/Al-Li composite is composed of two kind of Al-Li pweders with very different size ; (6) press-torsion, pressless sinter and hot is static press treatment will make original dendrite in Al-Li particles disappear, further enlargi metallurgical bonding region and incress the dinsity of composite.

        • Research on Automatic Mapping Method of Geologic Column

          Shao,YanLin,Shao,YaXiong,Xu,XiaoHong,He,ZhenMing,Li,GongQuan 보안공학연구지원센터 2015 International Journal of Hybrid Information Techno Vol.8 No.7

          The realization of the digital reservoir can greatly improve the information level of the petroleum corporation in hydrocarbon exploration and development, reduce risk and improve efficiency. Wells are the core object of digital reservoir management. Geologic column of drilling is the main carrier of multi-source information. Automatic mapping method and multi-source information fusion of geologic column have become a research hotspot of digital reservoir construction. This paper describes current situation of the domestic reservoir management and practical problem of geological maps digitizing. The thought and process of geologic column automatic mapping, which are based on spatial information technology, are submitted. The partition of information bar is settled on. The three-tier system architecture of automatic mapping system is established. With the support of XML technology, XSD is used to make the parameter template of geologic column automatic mapping and realize corresponding configuration wizard. UML is used to design the automatic mapping system. The lithological plate and geological symbols is coded to realize automatic mapping of corresponding drawing elements. The drawing methods of logging and petrophysical data are improved and the visualization of curve and numerical stick figure is achieved. The integration method of casting photos, core images and geologic column is submitted. For the secondary development technology of ArcGIS Engine, object-oriented program is adopted to realize automatic mapping of geologic ArcGIS Engine column. This paper provides a new way for the geologic column mapping during the process of digital reservoir construction.

        • KCI등재

          Time-related Mapping of Quantitative Trait Loci Controlling Dry Matter Accumulation and Partitioning in Rice

          Shao,Bo,Li,Zhi,Hong,Zhang,Shao,Qing,Li,Yang,Sheng,Li,You,Lin,Zhu,Ying,Guo,Zhu 한국유전학회 2008 Genes & Genomics Vol.30 No.4

          Grain yield is a result of dry matter accumulation and partitioning in rice (Oryza sativa L.). To characterize the genetic basis of dynamics of traits representing dry matter accumulation and partitioning as well as their relationships with each other, composite interval mapping of quantitative trait loci (QTL) was conducted based on time-related phenotypic data obtained from a population of 258 recombinant inbred lines (F12), which was derived from a cross between Lemont (japonica var.) and Teqing (indica var.). A total of twenty-three QTL were identified for leaf weight, stem weight and panicle weight at four measuring stages after heading. However, these three dynamic traits were observed to be controlled by different QTL (genes) at different growth stages. An extreme example was that at least three types of QTL (genes) might be involved in the development of panicle weight. Growth duration, which is closely related to dry matter accumulation and partitioning, was also identified to be significantly affected by four QTL. The close correlations among the studied four traits were explained to some extent by six co-located QTL. Nevertheless, it would be of great importance to distinguish the pleiotropic or linked effects of the co-located QTL. In addition, the chromosome regions or QTL identified in this study were compared with those previously reported for the same or related traits with the same RIL population.

        • Human Papillomavirus Vaccine Awareness, Acceptability, and Decision-Making Factors among Chinese College Students

          Wang,,Shao-Ming,Zhang,,Shao-Kai,Pan,,Xiong-Fei,Ren,,Ze-Fang,Yang,,Chun-Xia,Wang,,Zeng-Zhen,Gao,,Xiao-Hong,Li,,Man,Zheng,,Quan-Qing,Ma,,Wei,Zhao,,Fang-Hui,Qiao,,You-Lin,Sivasubramaniam,,Priya Asian Pacific Organization for Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

          Background: College students are recommended as the target groups for catch-up human papillomavirus (HPV) vaccination. Systematical exploration of awareness, acceptability, and decision-making factors of HPV vaccination among Chinese college students has been limited. Materials and Methods: A multi-center survey was conducted in mainland China between November 2011 and May 2012. College students aged 18-22 years were stratified by their grade, gender, and major for sampling. Socio-demographic and HPV-related information such as knowledge, perceptions, acceptability, and attitudes were collected through a questionnaire. Results: A total of 3,497 undergraduates completed the questionnaire, among which 1,686 were males. The acceptability of the HPV vaccine was high (70.8%). Undergraduates from high-level universities, at lower grade, or with greater prior knowledge of HPV vaccines showed higher acceptability of HPV vaccination ($p_{trend}$ <0.001). Additionally, undergraduates with vaccination experience outside the National Expanded Program on Immunization (OR=1.29; 95%CI: 1.10-1.51) or fear of HPV-related diseases (OR=2.79; 95%CI: 2.28-3.41) were more willing to accept HPV vaccination. General knowledge of HPV vaccine was low among undergraduates, and safety was a major concern (71.05%). The majority of students wished to pay less than 300RMB for HPV vaccine and chose the Chinese Center for Disease Control and Prevention as the most appropriate venue for vaccination. Conclusions: Although most undergraduates demonstrate positive attitudes towards HPV vaccination, challenges pertaining to introduction exist in China. Corresponding proactive education and governmental subsidy to do so are urgently needed by this age-group population. Suggestions and potential strategies indicated may help shape the future HPV vaccination program in China.

        • KCI등재SCOPUS

          Improving Cellulase Production in Trichoderma koningii Through RNA Interference on ace1 Gene Expression

          ( Wang Shao Wen ), ( Miao Xing ), ( Gang Liu ), ( Shao Wen Yu ), ( Juan Wang ), ( Sheng Li Tian ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.8

          Ribonucleic acid interference (RNAi) inhibits the expression of target genes in a sequence-specific manner, and shows potential for gene knockdown in filamentous fungi, in which the locus-specific gene knockout occurs in low frequency. In this study, the function of the repressor of cellulase expression I (ACEI) was verified in Trichoderma koningii (T. koningii) YC01 through RNAi, and ace1-silenced strains with improved cellulase productivity were obtained. An expression cassette that transcribed the interfering double-stranded RNA (dsRNA) of ace1 was constructed and transformed into T. koningii, and the transformants, in which the expression of ace1 was successfully silenced, were selected. As a result of the ace1 gene silencing, the expression levels of the main cellulase and xylanase genes were elevated, and the enhanced production of total proteins, cellulase, and xylanase was observed in the cultivation. In addition, the downregulation of ace1 resulted in an increasing expression of xyr1, but no clear variation in the expression of cre1, which suggested that ACEI acted as a repressor of the xyr1 transcription, but was not involved in the regulation of the cre1 expression. The results of this work indicate that ace1 is a valid target gene for enhancing enzyme production in T. koningii, and RNAi is an appropriate tool for improving the properties of industrial fungi.

        • KCI등재

          Study on the Undertaking Mode of High Tech Industry in Anhui Province : Based on the Perspective of Industrial Upgrading

          Shao,Mengmeng 한국유통과학회 2018 The Journal of Industrial Distribution & Business( Vol.9 No.5

          '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

          Purpose - The development of Anhui high-tech industry is the key to transforming the mode of economic development and optimizing the industrial structure. Research design, data, and methodology - The data of paper that is the data of Anhui province and the six provinces of central China from 2010 to 2015, which analyzed the present situation of High-tech industry in Anhui Province by means of data comparison and literature analysis, and then explored several possible modes of undertaking. Results - With the analysis of the current situation of the development of high-tech industry in Anhui Province, several possible modes for undertaking the transfer of high-tech industries in Anhui Province were explored: The mode of undertaking of Leading Enterprises + Industrial park, the mode of undertaking of differentiated development + Regional comprehensive exploitation and the mode of undertaking "Dynamic industrial Chain" + industrial cluster. Conclusions - Based on the perspective of industrial upgrading, this paper analyzes the shortcomings of Anhui Province in the traditional mode of undertaking industrial transfer and expounds the inherent requirements of innovation industry undertaking. Finally, on the basis of the above analysis, the author explored three possible modes of acceptance.

        • RNAi-based Knockdown of Multidrug Resistance-associated Protein 1 is Sufficient to Reverse Multidrug Resistance of Human Lung Cells

          Shao,,Shu-Li,Cui,,Ting-Ting,Zhao,,Wei,Zhang,,Wei-Wei,Xie,,Zhen-Li,Wang,,Chang-He,Jia,,Hong-Shuang,Liu,,Qian Asian Pacific Organization for Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

          Up-regulation of multidrug resistance-associated protein 1 (MRP1) is regarded as one of the main causes for multidrug resistance (MDR) of tumor cells, leading to failure of chemotherapy-based treatment for a multitude of cancers. However, whether silencing the overexpressed MRP1 is sufficient to reverse MDR has yet to be validated. This study demonstrated that RNAi-based knockdown of MRP1 reversed the increased efflux ability and MDR efficiently. Two different short haipin RNAs (shRNAs) targeting MRP1 were designed and inserted into pSilence-2.1-neo. The shRNA recombinant plasmids were transfected into cis-dichlorodiamineplatinum-resistant A549 lung (A549/DDP) cells, and then shRNA expressing cell clones were collected and maintained. Real time PCR and immunofluorescence staining for MRP1 revealed a high silent efficiency of these two shRNAs. Functionally, shRNA-expressing cells showed increased rhodamine 123 retention in A549/DDP cells, indicating reduced efflux ability of tumor cells in the absence of MRP1. Consistently, MRP1-silent cells exhibited decreased resistance to 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and DDP, suggesting reversal of MDR in these tumor cells. Specifically, MRP1 knockdown increased the DDP-induced apoptosis of A549/DDP cells by increased trapping of their cell cycling in the G2 stage. Taken together, this study demonstrated that RNAi-based silencing of MRP1 is sufficient to reverse MDR in tumor cells, shedding light on possible novel clinical treatment of cancers.

        • KCI등재SCOPUS

          Identification of Halohydrin Dehalogenase Mutants that Resist COBE Inhibition

          Shao-Yun,Chen,Xiu-Juan,He,Jian-ping,Wu,Gang,Xu,Li-Rong,Yang 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

          The biocatalytic cascade conversion of ethyl4-chloroacetoacetate (COBE) to ethyl (R)-4-cyano-3-hydroxybutyrate ((R)-HN) for the preparation of atorvastatinrepresents significant economic and environmental benefits,and is catalyzed by alcohol dehydrogenase and halohydrindehalogenase (HHDH). However, as the activity of HHDHis inhibited by COBE, the cascade reaction is an inefficientone-pot reaction. In this study, substrate inhibition kineticsanalysis was performed and the inhibition by COBE wasfound to be competitive reversible inhibition. Molecularsimulation analysis was used to determine the inhibitionmechanism by COBE. The results showed that COBEbound to the active center of HHDH via the formation ofhydrogen bonds with the OH groups of S132 and Y145. Site saturation mutagenesis of residues around the activesite and at the entrance of the access tunnel was performed,and two target mutant residues were identified, F136 andW249. Small focused mutagenesis on these two residueswas performed and the F136V/W249F mutant wassuccessfully found to relieve the activity inhibition ofHHDH by COBE. The half inhibiting concentration ofmutant F136V/W249F was found to be 20-fold higher thanwild-type HHDH. The efficiency of the multi-enzymaticone-pot system for the synthesis of (R)-HN from COBEusing mutant F136V/W249F was improved significantly.

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