S. Typhi, S. Typhimurium 및 S. Enteritidis 균의 분자역학적 연구

강연호,박미선,김성한,유재연,김호훈,이복권 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

단세포군 항체를 이용한 Shigella serotyping serum 개발(Ⅱ) : S. flexneri type Ⅰ,Ⅱ & type Ⅰ:6, group 6

정의범,이영희,김순남,박강수,이명숙,성원근,김종욱,홍승의,박정우 국립보건연구원 1994 국립보건원보 Vol.31 No.1

Fatigue performance of deepwater SCR under short-term VIV considering various S-N curves

Kim, D.K.,Choi, H.S.,Shin, C.S.,Liew, M.S.,Yu, S.Y.,Park, K.S. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.53 No.5

In this study, a method for fatigue performance estimation of deepwater steel catenary riser (SCR) under short-term vortex-induced vibration was investigated for selected S-N curves. General tendency between S-N curve capacity and fatigue performance was analysed. SCRs are generally used to transport produced oil and gas or to export separated oil and gas, and are exposed to various environmental loads in terms of current, wave, wind and others. Current is closely related with VIV and it affects fatigue life of riser structures significantly. In this regards, the process of appropriate S-N curve selection was performed in the initial design stage based on the scale of fabrication-related initial imperfections such as welding, hot spot, crack, stress concentration factor, and others. To draw the general tendency, the effects of stress concentration factor (SCF), S-N curve type, current profile, and three different sizes of SCRs were considered, and the relationship between S-N curve capacity and short-term VIV fatigue performance of SCR was derived. In case of S-N curve selection, DNV (2012) guideline was adopted and four different current profiles of the Gulf of Mexico (normal condition and Hurricane condition) and Brazil (Amazon basin and Campos basin) were considered. The obtained results will be useful to select the S-N curve for deepwater SCRs and also to understand the relationship between S-N curve capacity and short-term VIV fatigue performance of deepwater SCRs.

Similarities and differences between 6S RNAs from Bradyrhizobium japonicum and Sinorhizobium meliloti

Olga Y. Burenina,Daria A. Elkina,Anzhela Y. Migur,Tatiana S. Oretskaya,Elena Evguenieva-Hackenberg,Roland K. Hartmann,Elena A. Kubareva 한국미생물학회 2020 The journal of microbiology Vol.58 No.11

6S RNA, a conserved and abundant small non-coding RNA found in most bacteria, regulates gene expression by inhibiting RNA polymerase (RNAP) holoenzyme. 6S RNAs from α-proteobacteria have been studied poorly so far. Here, we present a first in-depth analysis of 6S RNAs from two α-proteobacteria species, Bradyrhizobium japonicum and Sinorhizobium meliloti. Although both belong to the order Rhizobiales and are typical nitrogen-fixing symbionts of legumes, their 6S RNA expression profiles were found to differ: B. japonicum 6S RNA accumulated in the stationary phase, thus being reminiscent of Escherichia coli 6S RNA, whereas S. meliloti 6S RNA level peaked at the transition to the stationary phase, similarly to Rhodobacter sphaeroides 6S RNA. We demonstrated in vitro that both RNAs have hallmarks of 6S RNAs: they bind to the σ70-type RNAP holoenzyme and serve as templates for de novo transcription of so-called product RNAs (pRNAs) ranging in length from ~13 to 24 nucleotides, with further evidence of the synthesis of even longer pRNAs. Likewise, stably bound pRNAs were found to rearrange the 6S RNA structure to induce its dissociation from RNAP. Compared with B. japonicum 6S RNA, considerable conformational heterogeneity was observed for S. meliloti 6S RNA and its complexes with pRNAs, even though the two 6S RNAs share ~75% sequence identity. Overall, our findings suggest that the two rhizobial 6S RNAs have diverged with respect to their regulatory impact on gene expression throughout the bacterial life cycle.

S-BRT 운행행태를 고려한 저상버스의 정차시간 예측모형

신소명,이수범,김영찬,박신형,유연승,최정훈,Shin, S.M.,Lee, S.B.,Kim, Y.C.,Park, S.H.,Yu, Y.S.,Choi, J.H. 한국안전학회 2021 한국안전학회지 Vol.36 No.1

This basic study introduces the concept of S-BRT and develops dwell time estimation models that consider road geometry and S-BRT characteristics for a signal operation strategy to meet the S-BRT's operational goal of high speed and punctuality. Field surveys of low-floor buses similar in shape to S-BRTs and data collection of passengers, station elements, vehicle elements, and other factors that can affect stop times were used in a regression analysis to establish statistically significant dwell time estimation models. These dwell time estimation models are developed by categorizing according to the locations of the signal or sidewalk that have the most impact on the dwell time. In this way, the number of people boarding and alighting the bus at the crowded door and the number of people boarding and alighting the bus at the front door considering the internal congestion was analyzed to affect the dwell time. The estimation dwell time models in this study can be used in the establishment of strategies that provide priority signals to S-BRTs.

Production of (S)-3-hydroxybutyrate by metabolically engineered Saccharomyces cerevisiae

Yun, E.J.,Kwak, S.,Kim, S.R.,Park, Y.C.,Jin, Y.S.,Kim, K.H. Elsevier Science Publishers 2015 Journal of biotechnology Vol.209 No.-

(S)-3-Hydroxybutyrate (S-3HB) can be used as a precursor for the synthesis of biodegradable polymers such as polyhydroxyalkanoate and stereo-specific fine chemicals such as antibiotics, pheromones, and drugs. For the production of S-3HB in yeast, the biosynthetic pathway of S-3HB from acetyl-CoA, consisting of the three enzymes, acetyl-CoA C-acetyltransferase (ACCT), acetoacetyl-CoA reductase (ACR), and 3-hydroxybutyryl-CoA thioesterase (HBT), was introduced into Saccharomyces cerevisiae. An engineered yeast strain overexpressing ERG10, hbd, and tesB genes not only exhibited enzyme activities of AACT, ACR, and HBT, but also produced S-3HB from ethanol. In order to increase the titer of S-3HB, a fed-batch fermentation based on pulse feeding of ethanol as a carbon source was performed, and a final S-3HB titer of 12.0g/L was achieved. This is the first report on the production of 3HB by engineered yeast, utilizing ethanol as the carbon source, suggesting that the industrially preferred S. cerevisiae can be a promising host for producing S-3HB.

Salmonella Typhi, Salmonella Typhimurium 및 Salmonella Enteritidis의 항균제 감수성 (1997)

신영학,유정식,김기상,정동준,오경수,이점규,이상원,이근영,박미선,이복권,김호훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

S nutrition alleviates salt stress by maintaining the assemblage of photosynthetic organelles in Kentucky bluegrass (Poa pratensis L.)

Park, S. H.,Lee, B. R.,Lee, J. H.,Kim, T. H. Springer Science + Business Media 2016 Plant growth regulation Vol.79 No.3

<P>To assess the roles of sulfur (S) nutrition in salt stress tolerance in Kentucky bluegrass (Poa pratensis L.). The plants grown in S-supplied or S-deprived condition for 4 weeks were exposed to salt stress with 100 mM NaCl or non-salt stress, respectively, for 21 days. Osmotic potential was significantly decreased by salt stress from day 14. Photosynthetic pigments such as chlorophyll and carotenoid were decreased by salt stress which was more severe in the absence of S, but their content was largely recovered in the presence of S-nutrition. The proteomic analysis of multi-protein complexes in the thylakoid by BN-PAGE showed that the expression of PSI, PSII and RuBisCo level was repressed under salt stress in the absence of S, whereas their expression was largely recovered by S supply. Enzymatic activity confirmed the responses of RuBisCo, estimated by the BN-PAGE, showing a decreased activity in S-deprived and/or salt stressed levels. The decreased RuBisCo activity was significantly related to S content as affected by S nutrition and/or salt stress. Significant relationship between S content and Na, K, Fe content was also observed. These results indicate that S-nutrition modulates the negative responses to salt stress tolerance in photosynthetic organelles of P. pratensis.</P>

Role of the Meckel’s Cartilage in Embryonic Mandibular Development of Mice

J. W Choi,S. B Han,J. H Sung,H. I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

Mecke!'s car t ilage is one of the ea rliest structu re to appear in a mandible derived from the lï rst branchi a l a rch and serves as the primorclium I"or the formation 01‘ mandible‘ mall eus. incus. and sphenomandibular li gament However. its direct role a nd the mechanism in mandibular clevelopment a re not well elucidated. 1'0 address t his Issue‘ we observed morphol ogical and histological changes and gene expression patterns in the Mecke!'s cart ilage 01" a cleveloping mouse. I"rom E13.5 to E18.5 embryos. using skeletal preparation samples a ncl routinely prepa red s lide secti ons for light mi croscopic observation in various sectional planes. The following methods were per |‘ ormecl : H&E staining I"or general hi st이 og i cal observation ‘ Von Kossafor detection of minerali zation. TRAP activ ity staining for locali zaLion 01’ osteoclastic cell s. immunohistochemistry for !Iα@-1 a ncl -9 forevaluati on of enzy matic activity 01" osteoclasLic cell s. a ncl in situ hybricli zation for detection of collagen type 1. Il. ancl X mRNA ex presslon‘ respecLively. AL E1 3.5 Mec kel's cartilage appeared as a V-shaped rod fused a t the micl line and thin minera li zed ma ndibular buccal plaLe was I"ormed lateral to. and at some clistance from. Meckel’s carti lage in an intramembranous ossi lïcation mocle. WiLh the progression of tooth development. t he Meckel’s in carti lage adjacent incisors revealecl hyperLrophi c chonclrocyte di ff"er entiation with minerali zation of the chondroid matrix. The Meckel’s car Li lage was replacecl with bone by o~ L eoc l asLs . showing strong immunoreact ivity for MMP- l ancl -9 from E16 5 Wi Lh ti me‘ Lhis bony replacement of Meckel's cartilage in an endochondral ossification mode was ex Lenclecl up Lo the mid-porLion of Lhe molar sockets til l EI8.5. The bony replacement of minera li zed hypertrophic chondrocyte zone expressing X collagen mHNA conLri buted to the formation of thick mandibular lingual plate . 1'hese f"i ndings suggesL LhaL mandibular formalion and development is closely relatecl with not only Mecke!'s carLi lage. buL also wiLh Lhc developing LooLh. and thaL C'erLai n in f"l uence from the developing tooth may play a role in detcrmin in g Lhe faLc of Meckel’s ca rLi lage cluring ma ndi bular development.