상업적 응유효소의 탈지유에 대한 단백질 분해 작용

신현수,김상범,임종우 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.6

상업적 단백질 분해 효소에 0.02% CaCl_2를 첨가하여 응유 활성화를 시킨 탈지유에 대한 분해 작용의 결과를 요약하면 다음과 같다. 다양한 효소별 가수분해 시간에 따른 가수분해도는 미생물 유래 효소와 trypsin은 pepsin과 papain W-40보다 높은 분해도를 나타냈다. 12% TCA 용액에 가용성인 NPN의 양은 trypsin이 가장 높은 분해도를 나타내었고 rennet과 pepsin이 가장 낮은 분해도를 보였다. 전기영동에 있어서 trypsin과 protease S는 α-lactalbumin을 분해하였고 papain w-40은 β-lactoglobulin을 미약하게 분해하였으며 neutrase 1.5는 90분 이후부터 α-lactalbumin과 β-lactoglobulin을 분해하였다. Rennet과 비교한 전기영동상에서는 rennet에 의해 분해 되지 않은 α_s-casein과 β-casein을 trypsin과 protease S가 다량 분해하였고 k-casein은 rennet에 비해 papain W-40이 상당 수준의 분해상을 나타내었다. 이상의 결과 가수분해도 및 NPN 양은 trypsin, neutrase 1.5 및 protease S가 다른 효소에 비해 높게 나타났으며, 전기영동상에서는 pepsin과 neutrase 1.5가 rennet과 유사한 경향을 나타내었다. Proteolytic activities of some commercial milk clotting enzymes(rennet, trypsin, pepsin, papain W-40, neutrase 1.5 and protease S) in bovine skim milk containing 0.02% CaCl_2 were determined by measuring DH(Degree of Hydrolysis), NPN(Non Protein Nitrogen) and by comparing patterns of SDS-PAGE(Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis). The DH of microbial enzymes(neutrase 1.5 and protease S) and trypsin in bovine skim milk were higher than those of pepsin and papain W-40. The amounts of NPN in the milk treated with trypsin and the other animal enzymes(rennet and pepsin) showed the highest and lowest degrees of proteolysis, respectively. SDS-PAGE showed that trypsin and protease S hydrolyzed α-lactalbumin and papain W-40 hydrolyzed β-lactoglobulin slightly, while neutrase 1.5 hydrolyzed both α-lactalbumin and β-lactoglobulin after treating for 90 min. Trypsin and protease S easily hydrolyzed α_s-casein and β-casein, which were not hydrolyzed by rennet. Papain W-40 hydrolyzed k-casein more than rennet as shown in SDS-PAGE. Based on the results of the experiments, the DH and NPN of trypsin, neutrase 1.5 and protease S were shown to be higher than those of the other enzymes. The SDS-PAGE patterns of papain W-40 and neutrase 1.5 were similar with that of rennet.

한우 포유모우의 산유량및 유조성분에 관한 연구 1 . 포유모우의 산유량 추정방법 확립에 관한 연구

강수원(S . W . Kang),정연후(Y . H . Chung),정창화(C . H . Chung),나승환(S . H . Na),손용석(Y . S . Son),백동훈(D . H . Baik) 한국축산학회 1989 한국축산학회지 Vol.31 No.3

This experiment was conducted to establish the estimating of dam`s milk producing capacity which forms the basis of Korean Native Cattle improvement for 3 years from 1982 to 1984. Four methods, including weight difference of the calf before and after nursing, hand milking and machine milking were compared with a new milking while the calf nurses, which the good points from the existing methods were extracted and then were developed. Fifty nine heads of nursing cow were tested to investigate dam`s milk yield. dam`s milk composition by the new milking method for 3 years from 1985 to 1987. The results of this experiment are summarized as follows: 1. This new method was made to use a portable vacuum·operated bucket milker in two quarters of each cow`s left or right udder while the calf suckles two quarters of his or her dam`s right or left udder. On this method. cows and calves were seperated at 8:00 am and at 5:00 pm the calves allowed to nurse. Cows and calves remained seperated over night. At 8:00 am and 5:00 pm the following day, the calves allowed to nurse one udder half while the rest was milked by machine. 2. On the comparison with methods estimating dam`s milk yield, the method by calf-weight difference before and after nursing wasn`t exact and good method in estimating dam`s milk yield and composition because not only this method was indirectly estimated by calf`s weight gain but also milk sample could not be taken. And also, the method by hand milking and machine milking could be surveyed only 60 days and 4 to 10 days, respectively, because dam`s milk yield was rapidly decreased with passing the milking day. However, by the new method, milking could be completely done without failure for 180 days and samples for analyzing the milk composition could be taken more easily and exactly than any other method. 3. Daily average and total milk yield of fifty nine heads investigated by this new method for 180 days was 3.48 and 625.8kg, respectively. 4. In the milk composition, total solids, far, protein, lactose and ash content was 14.21, 4.64, 4.07, 4.21 and 0.89 percent, respectively.

Role of the Meckel’s Cartilage in Embryonic Mandibular Development of Mice

J. W Choi,S. B Han,J. H Sung,H. I Shin 대한구강악안면병리학회 2005 대한구강악안면병리학회지 Vol.29 No.5

Mecke!'s car t ilage is one of the ea rliest structu re to appear in a mandible derived from the lï rst branchi a l a rch and serves as the primorclium I"or the formation 01‘ mandible‘ mall eus. incus. and sphenomandibular li gament However. its direct role a nd the mechanism in mandibular clevelopment a re not well elucidated. 1'0 address t his Issue‘ we observed morphol ogical and histological changes and gene expression patterns in the Mecke!'s cart ilage 01" a cleveloping mouse. I"rom E13.5 to E18.5 embryos. using skeletal preparation samples a ncl routinely prepa red s lide secti ons for light mi croscopic observation in various sectional planes. The following methods were per |‘ ormecl : H&E staining I"or general hi st이 og i cal observation ‘ Von Kossafor detection of minerali zation. TRAP activ ity staining for locali zaLion 01’ osteoclastic cell s. immunohistochemistry for !Iα@-1 a ncl -9 forevaluati on of enzy matic activity 01" osteoclasLic cell s. a ncl in situ hybricli zation for detection of collagen type 1. Il. ancl X mRNA ex presslon‘ respecLively. AL E1 3.5 Mec kel's cartilage appeared as a V-shaped rod fused a t the micl line and thin minera li zed ma ndibular buccal plaLe was I"ormed lateral to. and at some clistance from. Meckel’s carti lage in an intramembranous ossi lïcation mocle. WiLh the progression of tooth development. t he Meckel’s in carti lage adjacent incisors revealecl hyperLrophi c chonclrocyte di ff"er entiation with minerali zation of the chondroid matrix. The Meckel’s car Li lage was replacecl with bone by o~ L eoc l asLs . showing strong immunoreact ivity for MMP- l ancl -9 from E16 5 Wi Lh ti me‘ Lhis bony replacement of Meckel's cartilage in an endochondral ossification mode was ex Lenclecl up Lo the mid-porLion of Lhe molar sockets til l EI8.5. The bony replacement of minera li zed hypertrophic chondrocyte zone expressing X collagen mHNA conLri buted to the formation of thick mandibular lingual plate . 1'hese f"i ndings suggesL LhaL mandibular formalion and development is closely relatecl with not only Mecke!'s carLi lage. buL also wiLh Lhc developing LooLh. and thaL C'erLai n in f"l uence from the developing tooth may play a role in detcrmin in g Lhe faLc of Meckel’s ca rLi lage cluring ma ndi bular development.

수집형 감자 수확기 개발을 위한 굴취·이송 요인실험

이상희 ( S. H. Lee ),김영근 ( Y. K. Kim ),강태경 ( T. G. Kang ),김성우 ( S. W. Kim ),최용 ( Y. Choi ),전현종 ( H. J. Jun ),최일수 ( I. S. Choi ),현창식 ( C. S. Hyun ) 한국농업기계학회 2016 한국농업기계학회 학술발표논문집 Vol.21 No.2

감자는 국내에서 재배되는 주요 밭작물로 감자의 소비량은 증가하고 있지만, 재배면적은 2000년 29,400ha에서 2014년 22,000ha로 감소하고 있는 추세이며 생산량 또한 2000년 704,6000톤에서 2014년 590,000톤으로 감소하고 있다. 현재 감자의 수확작업은 굴취기를 이용하여 굴취한 후 인력으로 수집하는 형태로 감자 생산 총 노동시간 58.0hr/10a 중 수집 작업에 소요되는 시간이 17.1hr/10a에 달하며 농촌 노동력 감소와 고령화로 인해 수집형 감자 수확기 개발 요구가 높아지고 있다. 따라서 본 연구에서는 감자의 생력재배(Labor saving cultivation)를 높이기 위해 수집형 감자수확기개발을 위한 굴취날 각도 및 이송속도에 따른 요인실험을 수행 하였다. 수집형 감자 수확기는 트랙터 부착형으로 굴취날 각도 3수준(20, 23, 26°), 이송속도 3수준(0.3, 0.4, 0.5m/s)으로 조절할 수 있도록 제작하였다. 굴취날은 각도 별로 3개를 제작하여 날을 교체하는 방식으로 제작하였고, 시험은 평창 감자밭에서 이루어졌으며 시험포장의 토성은 양토였다. 굴취율은 3m구간 굴취 후 굴취된 개수와 미굴취 감자의 개수를 비교하였으며 3반복 측정 하였다. 이송속도는 모터 회전수를 제어할 수 있는 모터 컨트롤러를 따로 제작하여 기체는 정지시키고 이송부만 작동하며 실험하였다. 이송속도 별로 1회에 시료 50개를 투입하여 총 3반복 실험하였으며 표피손상과 형태가 파손된 것을 손상으로 간주하였다. 실험에 사용된 감자의 물성은 크기와 무게를 조사하였으며 평균 가로 75.4±6.26mm, 세로 62.5±5.3mm, 높이 81.7±7.67mm, 무게는 230.7±45.6g이었다. 수집형 감자 수확기의 굴취 및 이송 요인별로 시험하여 감자 굴취율, 이송손상 및 수집손상을 조사한 결과 굴취날 각도에 따른 굴취율은 감자수집에 미치는 영향이 적은 것으로 나타났다. 굴취날 각도 20, 23°에서는 약 99%로 수집되었으며, 26°의 굴취각에서는 모두 수집되었다. 또한 이송속도에 따른 이송부 및 수집부의 감자손상 및 손상률 시험결과 이송부손상률은 0.3m/s에서 2.67%, 0.4m/s에서 3.33%, 0.5m/s에서 6.67%로 0.5m/s의 이송속도에서 손상율 증가폭이 크게 상승하였다. 수집부 손상율 또한 0.3m/s에서 2%, 0.4m/s에서 2.67%, 0.5m/s에서 약 6.67%로 0.5m/s의 이송속도에서 증가폭이 크게 상승하였다. 따라서 본 연구의 수집형 감자 수확기는 작업속도, 감자손상 및 손상율을 고려할 때 0.4m/s의 이송속도에서 수확 시 가장 적절할것으로 판단하였다.

기계체조선수(器械體操選手)의 총지방량(總脂肪量)에 관(關)한 연구(硏究)

白元俊(W.J.Paik),金尙民(S.M.Kim),兪承熙(S.H.Yoo) 한국체육학회 1975 한국체육학회지 Vol.11 No.-

We computed the % Fat by the measured values of skinfold thickness at 4 sites; back, waist, abdomen, arm with 20 male gymnasic players(8 Kyung Hee high school, 12 Kyung ung Hee university players) and with 16 Female gymnastic player (8 Kyung He girl’s high school players, 8 Kyung Hee university players) hat all (total) 36 players were executed.Calculation Formula● high school players% Fat=1.324×Mean Skinfold thickness(mm) -0.045● Male College players% Fat=0.911×Mean Skinfold thickness(㎜)+8.1● Female high school and college players% Fat=0.58×Mean skinfold thickness(㎜)+14.311. The player’s average Skinfold thickness of 4 sites was less that of non-athletic persons.2. Both of male and Female gymnastic players showed the maximum value on the among 4 sites, while non-athltic males showed in back and Female in Arms, the maximum value.3. The Female player’s Skinfold thickness was thicker than that of male about 5.8㎜.4. The Kyung Hee high school gymnastic players skinfold thickness is as follws;back 6.9㎜waist 8.3㎜abdomen 5.9㎜arm 4.8㎜, the skinfold thickness averaged 6.3㎜and the Kyung Hee university gymnastic player’sSkinfold thickness is as follows;back 6.5㎜waist 8.9㎜abdomen 5.8㎜arm 4.7㎜, the Skinfold thickness averaged 6.4㎜5. The Kyung Hee girl’s High school player’s skinfold thickness is as follows;back 9.6㎜waist 12.1㎜abdomen 11.9㎜arm 8.3㎜, the Skinfold thickness averaged 10.1㎜ and the women’s skinfold thikness(Kyung Hee university gymnastic players) is as follows;back 10.8㎜waist 16.7㎜abdomen 11.0㎜arm 11.3㎜, the skinfold thickness averaged 12.4㎜6. The male players % Fat 11.7% (High school players 8.3%, college players 14.0%) and Female player’s % Fat was 2.8% (girl’s High school player’s 20.1%, colleye players 21.5%)7. Both of male and Female gymnastic player’s % Fat was less that of non-athletic persons and college player’s % Fat was more than that of high school players.

중부 평야지에서 사일리지용 옥수수 품종 선발에 관한 연구 : 파종시기가 사일리지용 옥수수의 생육특성 및 수량에 미치는 영향 Effect of planting time on the growth and yield of silage corn

김준식,김원호,김원영,김영진,한학석,김맹중 한국축산학회 1998 한국축산학회지 Vol.40 No.1

This experiment was conducted at theForage Research Farm of the Beef and Dairy Cattle Division, National Livestock Research Institute, Songhwan in 1992 and 1995 to investigate the effects of planting time (April 15, May 11, June 5) on the growth and yield of silage com(Zea mat's L.). In the plant height, P3144W and DK729 were taller than the others, as 301.6, and 296.3cm, respectively, and P3282 was the shortest as 247.7㎝ at the first planting time. P3144W and DK729 were taller than the others, as 303.6㎝, 281.7㎝, respectively, and P3282 was the shortest as 249.6㎝ at the second planting time. P3144W was the tallest as 276.9㎝. DK689 was the shortest as 246.1㎝ at the third planting time. The ear heights of P3144W and P3282 were 137.3 and 132.7㎝, respectively, and Suweon 19 was the shortest as 116.4㎝ at the first planting time. Those of P3144W and P3282 were as 135.9, 134.4㎝, respectively, and Suwoen 19 was the shortest as 112.1㎝ at the second planting time. P3144W was the tallest as 111.0㎝ and Suweon 19 was the shortest as 94.2㎝ at the third planting time. In the dry matter yield per ha, P3352, DK729 and P3144W were higher than the others as 18,957, 18,840, 18,743㎏, respectively, and P3282 was the lowest as 13,863㎏ at the first planting time. 64743 and P3352 were 18,183 and 17,874㎏, respectively, and Suwoen 19 was the lowest as 15,007㎏ at the second planting time. P3144W was the highest as 19,112㎏ and Suwnen 19 was the lowest as 14,793㎏ at the third planting time. The TDN yield per ha, of P3352, P3144W and DK729 were 13,552, 13,281 and 13,252㎏, respectively, and P3282 was the lowest as 9,793㎏ at the first planting time. 64743 and P3352 were higher than the others as 13,149 and 13,009kg, respectively, and Suwoen 19 was the lowest as 10,872㎏ at the second planting time. P3144W was the highest as 13,521㎏ and Suwven 19 was the lowest as 10,392㎏ at the third planting time.

早期破膜에 關한 臨床的 考察

許湘,吳文愛,安東源,申吳永 대한산부인과학회 1972 Obstetrics & Gynecology Science Vol.15 No.7

<i>S100A9</i> and <i>EGFR</i> gene signatures predict disease progression in muscle invasive bladder cancer patients after chemotherapy

Kim, W. T.,Kim, J.,Yan, C.,Jeong, P.,Choi, S. Y.,Lee, O. J.,Chae, Y. B.,Yun, S. J.,Lee, S. C.,Kim, W. J. Oxford University Press 2014 Annals of Oncology Vol.25 No.5

<P>In our previous gene expression profile analysis, IL1B, S100A8, S100A9, and EGFR were shown to be important mediators of muscle invasive bladder cancer (MIBC) progression. The aim of the present study was to investigate the ability of these gene signatures to predict disease progression after chemotherapy in patients with locally recurrent or metastatic MIBC. Patients with locally advanced MIBC who received chemotherapy were enrolled. The expression signatures of four genes were measured and carried out further functional analysis to confirm our findings. Two of the four genes, S100A9 and EGFR, were determined to significantly influence disease progression (P = 0.023, 0.045, respectively). Based on a receiver operating characteristic curve, a cut-off value for disease progression was determined. Patients with the good-prognostic signature group had a significantly longer time to progression and cancer-specific survival time than those with the poor-prognostic signature group (P < 0.001, 0.042, respectively). In the multivariate Cox regression analysis, gene signature was the only factor that significantly influenced disease progression [hazard ratio: 4.726, confidence interval: 1.623-13.763, P = 0.004]. In immunohistochemical analysis, S100A9 and EGFR positivity were associated with disease progression after chemotherapy. Protein expression of S100A9/EGFR showed modest correlation with gene expression of S100A9/EGFR (r = 0.395, P = 0.014 and r = 0.453, P = 0.004). Our functional analysis provided the evidence demonstrating that expression of S100A9 and EGFR closely associated chemoresistance, and that inhibition of S100A9 and EGFR may sensitize bladder tumor cells to the cisplatin-based chemotherapy. The S100A9/EGFR level is a novel prognostic marker to predict the chemoresponsiveness of patients with locally recurrent or metastatic MIBC.</P>

O/W · W/O Emulsion의 Tribology 특성 연구

이봉구,임웅빈,오성모 원광대학교 생산 및 자동화기술연구소 1995 생산.자동화기술연구소논문집 : 원광대 Vol.- No.2

We have been so much interested in the lubrication of emulsion property recently. In particular, O/W and W/O type emulsion have a good property to be used in the Lubricative situation of several services. Therefore, in this section we have studied about the lubricational characteristics in Neat Oil, O/W and W/O type emulsion using the Falex, wear test machine. The result is as follows. 1. In the case of O/W and W/O type emulsion, the beginning friction coefficient than Neat Oil, but it becomes more excellent as the time passes. especially, the W/O type becomes the more excellent. 2. As the load increase, a change in temperature shows to us be nearly similar and all of them, the O/W type is the most excellent in the temperature characteristic. 3. In the beginning, the anti-wear characteristic of emulsion lowers, but after the some load has stability, O/W and W/O type is excellent in order of anti-wear characteristic. 4. In the regular hours, a change in the emulsion becomes settled, so the character of temperature is superior. specially, O/W type is predominant. Therefore, the emulsion in case of the O/W and W/O type are more excellent than Neat oil in the thermal stability and can improve the characteristics of tribology.

Salmonella Typhi, Salmonella Typhimurium 및 Salmonella Enteritidis의 항균제 감수성 (1997)

신영학,유정식,김기상,정동준,오경수,이점규,이상원,이근영,박미선,이복권,김호훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-