http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Comparison of WSSV (white spot syndrome virus) DNA using Different oligonucleotide primers
Seok Ryel Kim,Yeong Jin Kim,Sung Ju Jung,Myung Joo Oh 전남대학교 수산과학연구소 2006 수산과학연구소논문집 Vol.15 No.2
White spot syndrome (WSS) is a viral disease that affects on most of the commercially cultivated shrimp species in worldwide. WSSV causing white spot syndrome in Korea were genetically similar to PRDV in Japan. In this study, we compared PCR products of WSSV with those of PRDV, using their specific oligonucleotide primers. As a result of sequence analysis, amplified PCR products were almost identical to the described sequences used in this experiment. Therefore, the results obtained in this study suggest that WSSV causing white spot syndrome in Korea were genetically similar to PRDV in Japan. Naturally, more detailed studies will be needed to identify the taxonomic correlation between these viruses.
한창율(Ryel Chang Han) 미래학회 2018 미래학회 학술대회 자료집 Vol.2018 No.2
인터넷 커뮤니티는 수많은 네티즌들이 사회적 콘텐츠를 생산 하는 공간이다. 공공이슈와 정책문제에 대한 뉴스 담론도 인터넷 커뮤니티를 통해 배포 확산된다. 사회적 공론화 과정도 인터넷 커뮤니티를 통해 거치게 된다. 이는 특정 인터넷 커뮤니티를 활용한 공공이슈와 정책문제에 대한 공론화가 정치 경제적 파급 효과를 준다. 본 연구는 뉴스 담론이 특정 인터넷 커뮤니티를 통해 어떻게 재구성되고, 유포되면서 미치는 사회적 영향 분석이다. 이를 위해 먼저, 특정 인터넷 커뮤니티의 태생 과정과 여기에서 활동하는 네티즌들의 특성을 파악해 본다. 이후 특정 인터넷 커뮤니티가 공공이슈와 정책과정을 굴절시키는지 살펴본다. 본 연구과정에서는 뉴스 담론을 굴절 시키는 인터넷 커뮤니티에는 특정 프레임이 작용한다는 가설을 수립했다. 이를 위해 인터넷 커뮤니티 게시글에 나타난 프레임 변화를 구체적으로 분석해 보고, 프레임 변화를 구체적으로 분석하기 위해 연역적 접근 방식인 Semetko와 Valkenburg(2000) 프레임 연구방식을 적용해 본다.
Detection of Norovirus in Contaminated Ham by Reverse Transcriptase-PCR and Nested PCR
Seok Ryel Kim,Duwoon Kim,Ki-Sung Kwon,In-Gyun Hwang,Myung-Joo Oh 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.3
In order to enhance the efficacy of norovirus detection by reverse transcriptase-polymerase chain reaction (RT-PCR) and nested PCR, this study developed a norovirus mRNA concentration method using poly oligo dT-conjugated magnetic beads. An efficient norovirus detection protocol was performed on commercial ham using 2 viral elution buffers (glycine buffer and Tris beef extract buffer) and 2 concentration solutions [polyethylene glycol (PEG) and zirconium hydroxide]. The different approaches were verified by RT-PCR and nested PCR. This method was performed on ham in less than 8 hr by artificial inoculation of serial dilutions of the virus ranging from 1,000 to 1 RT-PCR unit/mL. The viral extraction and concentration method had 10-fold higher sensitivity using the combination of Tris beef extract buffer and PEG as compared to glycine buffer and zirconium hydroxide. This method proved that RT-PCR and nested PCR have the sensitive ability to detect norovirus in commercial ham, in that norovirus was successfully detected in artificially contaminated samples at a detection level as low as 1-10 RT-PCR unit/mL. Overall, such a detection limit suggests this protocol is both quick and efficient in terms of its potential use for detecting norovirus in meat products.
Antimicrobial Effects of Chemical Disinfectants on Fish Pathogenic Bacteria
Seok-Ryel Kim,Kyung-Hee Park,Duwoon Kim,Sung-Ju Jung,So-Yong Kang,Myung-Joo Oh 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.5
This study was to examine the potential disinfection efficiencies of 10 compounds by determining their antimicrobial capacity and ichthyotoxicity. Antimicrobial effects against Vibrio sp., Edwadsiella tarda, Streptococcus sp., and Staphylococcus sp. were tested using 10 different disinfectants; hydrogen peroxide, sodium hypochlorite, chlorine dioxide, povidon iodine, formaldehyde, glutaraldehyde, quaternary ammonium compounds (QACs), didecyl dimethyl ammonium chloride (DDAC), ortho-dichlorobenzen, and copper sulfate. Chlorine dioxide (ClO<sub>2</sub>) containing 5% ClO<sub>2</sub> and copper sulfate had no effects on bactericidal activity, while the other disinfectants resulted in 99.99% bactericidal activity against 4 strains of fish pathogenic bacteria. The ichthyotoxicity of the 10 disinfectants was investigated using 3 kinds of fish species; flounder (Paralichthys olivaceus), rockfish (Sebastes pachycephalus), and black sea bream (Acanthopagrus schlegelii). Median lethal concentration (LC<sub>50</sub>) values of the 10 disinfectants were estimated to determine toxicity ranges of the doses within 24 hr. Among test disinfectant solutions, hydrogen peroxide showed the highest LC<sub>50</sub> in flounder (201.3), rockfish (269.7), and black sea bream (139.3 ppm). DDAC revealed the lowest LC<sub>50</sub> in flounder (2.1), rockfish (1.0), and black sea bream (1.5 ppm). These results suggest that DDAC, quaternary ammonium compounds, glutaraldehyde, and sodium hypochlorite are effective disinfectants for fish and bacterial species examined in this study.