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Samia Siddique,RubinaNelofer,Quratulain Syed,Ahmad Adnan,Fahim Ashraf Qureshi 한국응용생명화학회 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.5
Avermectin is an environment friendly bio-insecticide. Optimization of the culture conditions for avermectin B1bproduction has not been carried out before using Artificial NeuralNetwork (ANN) method. The present work is therefore conductedto optimize some important factors including yeast extract,MgSO4.7H2O, and temperature for the avermectin B1b productionusing ANN methodology from Streptomyces avermitilis DSM41445. The optimum levels for the yeast extract, MgSO4.7H2O,and temperature were 16.0 (g/L), 5.0 (g/L) and 32oC respectively. Maximum effect was observed by yeast extract. Avermectin B1byield was increased up to 150% after optimization. ANN wasfound to be a powerful technique for the optimization andprediction of avermectin B1b production from Streptomycesavermitilis DSM 41445.