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Shang, Jiang-Hua,Xu, Ru-Xiang,Jiang, Xiao-Dan,Zou, Yu-Xi,Qin, Ling-Sha,Cai, Ying-Qian,Yang, Zhi-Jun,Zheng, Xing,Cui, Sheng Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9
The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed out from oviducts (flushed) was carried out. The results showed that maturation and enucleation rates of aspirated oocytes were 70.7% and 69.2%, significantly lower than 95.3% (p<0.01) and 83.6% (p<0.05), respectively, from flushed oocytes. However, following enucleation of matured oocytes as ooplasm recipients for SCNT, no difference was recorded in fusion and cleavage rates, as well as blastocyst development from cleaved embryos or hatching of blastocysts between aspirated and flushed groups. Additionally, some matured aspirated and flushed oocytes were also used for immediate parthenogenetic activation and the resulting embryo development was not significantly different. Results from this study show the following: i) the majority of oocytes aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration are matured and can be used directly as ooplasm recipients for SCNT; ii) the reconstructed embryos derived from these oocytes have similar in vitro developmental ability to those flushed from the oviducts.
IMPACTS OF SHELLFISH CULTURE ON THE ECOSYSTEM IN SEMI-ENCLOSED BAYS
Ming Yuan Zhu,Ru Bao Ji,Shang Chen,Xue Lei Zhang 한국해안해양공학회 1999 학술강연회 발표논문초록집 Vol.1 No.1
Stimulated by high economic profits, the industries of shellfish culture have grown rapidly during the last two decades. The expansion of mariculture adds pressure on the environment and associated ecosystem. The potential impacts of the cultivation include reduction of primary productivity due to the consumption of large quantities of phytoplankton, changes of water current movement resulted from the semi-permeation 'Island' effects of the suspended culture, and production of a large amount of faeces and pseudofaeces which may enhance the importance of benthic ecosystem in geo-biological cycling.
Guang-Xun Lin,Li-Ru He,Jin-Niang Nan,Wen-Bin Xu,Keyi Xiao,Zhiqiang Que,Shang-Wun Jhang,Chien-Min Chen,Ming-Tao Zhu,Gang Rui 대한척추신경외과학회 2024 Neurospine Vol.21 No.1
Objective: This meta-analysis aims to refine the understanding of the optimal choice between different cage shapes in transforaminal lumbar interbody fusion (TLIF) by systematically comparing perioperative data, radiological outcomes, clinical results, and complications associated with banana-shaped and straight bullet cages. Methods: A meticulous literature search encompassing PubMed, Embase, Scopus, Web of Science, China Knowledge Network, and Wanfang Data was executed up to October 5, 2023. Inclusion criteria focused on studies comparing banana-shaped and straight bullet cages in TLIF. The quality of included studies was assessed using appropriate tools such as the Newcastle-Ottawa Scale (NOS) for nonrandomized studies. Rigorous evaluations were performed for radiographic outcomes, including disc height (DH), segmental lordosis (SL), lumbar lordosis (LL), subsidence, and fusion rates. Clinical outcomes were meticulously evaluated using visual analogue scale (VAS), Oswestry Disability Index (ODI), and complications. Results: The analysis incorporated 7 studies, involving 573 patients (297 with banana-shaped cages, 276 with straight cages), all with NOS ratings exceeding 5 stars. No statistically significant differences were observed in operative time, blood loss, or hospitalization between the 2 cage shapes. Banana-shaped cages exhibited greater changes in DH (p = 0.001), SL (p = 0.02), and LL (p = 0.01). Despite statistically higher changes in ODI for straight cages (26.33, p < 0.0001), the actual value remained similar to banana-shaped cages (26.15). Both cage types demonstrated similar efficacy in VAS, complication rates, subsidence, and fusion rates. Conclusion: Although banana-shaped cages can excel in restoring DH, SL, and LL, straight bullet cages can provide comparable functional improvements, pain relief, and complication rates.
Han-Lu Gao,Xuan Wang,Hong-Ru Sun,Jun-De Zhou,Shang-Qun Lin,Yu-Hang Xing,Lin Zhu,Hai-Bo Zhou,Ya-Shuang Zhao,Qiang Chi,Yu-Peng Liu 거트앤리버 소화기연관학회협의회 2018 Gut and Liver Vol.12 No.2
Background/Aims: Methylation status plays a causal role in carcinogenesis in targeted tissues. However, the relationship between the DNA methylation status of multiple genes in blood leukocytes and colorectal cancer (CRC) susceptibility as well as interactions between dietary factors and CRC risks are unclear. Methods: We performed a case-control study with 466 CRC patients and 507 cancer-free controls to investigate the association among the methylation status of individual genes, multiple CpG site methylation (MCSM), multiple CpG site heterogeneous methylation and CRC susceptibility. Peripheral blood DNA methylation levels were detected by performing methylation-sensitive high-resolution melting. Results: Total heterogeneous methylation of CA10 and WT1 conferred a significantly higher risk of CRC (adjusted odds ratio [ORadjusted], 5.445; 95% confidence interval [CI], 3.075 to 9.643; ORadjusted, 1.831; 95% CI, 1.100 to 3.047; respectively). Subjects with high-level MCSM (MCSM-H) status demonstrated a higher risk of CRC (ORadjusted, 4.318; 95% CI, 1.529 to 12.197). Additionally, interactions between the high-level intake of fruit and CRH, WT1, and MCSM on CRC were statistically significant. Conclusions: The gene methylation status of blood leukocytes may be associated with CRC risk. MCSM-H of blood leukocytes was associated with CRC, especially in younger people. Some dietary factors may affect hypermethylation status and influence susceptibility to CRC.
Shi-Man Du,Bing-Bing Shang,Xiao-Ru Zhang,Fu Feng,Sheng-Hui Zhang,Bao-Ping Qi 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.9
A Hg2+-related quenching photoluminescence (PL) sensor was fabricated based on nitrogen-doped graphene quantum dots (N-GQDs) as the luminescent agent and glutathione as the masking agent to detect Hg2+ in tap water. The addition of Hg2+ significantly reduced the PL intensity of N-GQDs, which was attributed to coordination reaction inducing the aggregation of N-GQDs. The Hg2+-related quenching PL sensor with glutathione as the masking agent has good selectivity and accuracy. The sensor showed a linear relationship ranging from 0.5 to 110?nM with the detection limit 0.08?nM (S/N = 3). The proposed method was applied to the determination of Hg2+ in tap water, and the results were consistent with atomic absorption spectroscopy (AAS).
Lived Experience among Patients Newly Diagnosed with Lung Adenocarcinoma Stage IV within One Year
Shih, Whei-Mei Jean,Hsu, Hsiu-Chin,Jiang, Ru-Shang,Lin, Mei-Hsiang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15
Background: lung cancer (LC) is the fifth of the 10 leading causes of death in the world. LC is in first place for cancer-related mortality for both males and females in Taiwan. It is one of the most difficult cancers to treat and is often diagnosed at a late stage. Patients with stage IV are often unprepared for the diagnosis. Materials and Methods: To explore lived experience among patients newly diagnosed with lung adenocarcinoma stage IV within one year. Results: Twelve participants were recruited in this study. Content analysis of the interviews revealed four themes: (1) emotional roller coaster, (2) trying to find out causes, (3) adjusting my lifestyle, and (4) cancer fighter. Conclusions: This study provides new insight into the experiences of lung cancer patients y with newly diagnosed lung adenocarcinoma stage 4. These results will inform future supportive care service development and intervention research for patients with advanced stage cancer.