http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Roopali N Bhoite,Navya PN,Pushpa S Murthy 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.suppl1
Extracellular β-glucosidase was produced using coffee pulp as a sole carbon source by Penicillium verrucosum by solid state fermentation and 897.36±59 U/g enzyme activity was obtained. Increase in 2.21-fold of enzyme activity on optimizing the bioprocess parameters by response surface methodology based on central composite rotatable design is illustrated. Maximum production level of 1,991.17 U/g was obtained with optimum values of pH 4.2, moisture 66.8%, and fermentation duration of 56 h. The enzyme was partially purified and the enzyme activity was optimum at 50oC temperature and at pH 6. The metal ions such as Mg2+, Zn2+, Ca2+, K+, detergents, and chelator such as EDTA were effective and further increased the β-glucosidase activity. On application of β-glucosidase for simultaneous saccharifiation and fermentation, 3.3% ethanol was obtained. Thus, this study provides insight on exploitation of P. verrucosum for synthesis of of β-glucosidase using coffee pulp which is available abundantly in coffee processing industries.
Hepatic basidiobolomycosis masquerading as cholangiocarcinoma: a case report and literature review
Roopali Sehrawat,Nalini Bansal,Ajitabh Srivastava,Dharmender Malik,Vivek Vij 대한간암학회 2023 대한간암학회지 Vol.23 No.2
Basidiobolus ranarum is known to cause subcutaneous mycoses; however, rare cases of hepatic and gastrointestinal involvement by basidiobolomycosis have been reported. Hepatic basidiobolomycosis may be confused with a carcinoma on imaging, and histological examination and fungal culture can help distinguish between these two. We report a rare case of basidiobolomycosis in a 16-year-old male with liver and gastrointestinal involvement.
Graphene-based nanomaterial system: a boon in the era of smart nanocarriers
Jha Roopali,Singh Amit,Sharma Pramod Kumar,Porwal Omji,Fuloria Neeraj Kumar 한국약제학회 2021 Journal of Pharmaceutical Investigation Vol.51 No.3
Background Owing to its exceptional electronic, mechanical, thermal, as well as optoelectronic characteristics, graphene—a planar sheet with one-atom thickness and made of carbon atoms closely packed in quite a honeycomb crystal lattice—has already earned special consideration. The investigated characteristics or even applications of such a two-dimensional configuration of the structure of carbon have already created novel innovative opportunities in place of all of the future devices as well as nanosystems. Area covered These graphene systems have also been recognized as a potential drug-delivery carrier intended for target specific or even controlled drug delivery, which could be designed by utilizing various internal or external stimuli. Expert opinion This review predominantly focuses on graphene’s synthesis and derivative, design of stimuli-responsive nanosystems, and applications in various fields. It is highlighted that with remarkable progress in graphene-related research, graphene’s overall execution has a long road ahead to go.
김진영,( Roopali Upadhyay ),홍은영,이선구,서주현,김병기 한국공업화학회 2018 한국공업화학회 연구논문 초록집 Vol.2018 No.0
A key point of protein stability engineering is to identify specific target residues that do not harm original activity of the protein. Here, we propose a method, called RiSLnet (Rapid identification of Smart mutant Library using residue network), to identify such residues by combining network analysis for protein residue interactions, identification of conserved residues, and evaluation of relative solvent accessibility. Application to lysine decarboxylase (CadA) confirmed its accuracy and effectiveness. RiSLnet successfully identified beneficial mutants with decent (~60%) accuracy and significantly reduced the number of candidate residues (~99%) for mutation. Finally, a combinatorial mutant designed by RiSLnet and in silico saturation mutagenesis resulted in an increase of half-life (T1/2) to 114.9 min at 58°C, which is approximately two-fold higher than that of the wild-type.
Hong, Eun Young,Kim, Jin Young,Upadhyay, Roopali,Park, Byung Jun,Lee, Jong Min,Kim, Byung-Gee Elsevier 2018 Journal of biotechnology Vol.281 No.-
<P>Ornithine decarboxylase (ODC) converts C5 ornithine into C4 putrescine, a monomer for polyamide synthesis. However, ODC also has minor activity towards cell metabolite C6 lysine and yields C5 cadaverine. The accumulation of cadaverine in the reaction solution causes increase in the operational cost of subsequent distillation process for putrescine purification. Here, to increase ODC substrate specificity toward ornithine over lysine, molecular modelling and protein network analysis, specifically k-clique community analysis, around the substrate tunnel were performed. This resulted in a mutant with two-fold increase in substrate specificity (ornithine versus lysine) without losing its original activity towards ornithine (k(cat)/K-M = 61.5 s(-1) mM(-1)), compared to the native enzyme. When this mutant was used for putrescine synthesis, 31.6 g/L putrescine (based on 51.5 g/L ornithine) titer was achieved, while 0.007 g/L (based on 2.57 g/L lysine) cadaverine was produced. This corresponds to four-fold decrease in cadaverine yield compared to the native ODC.</P>