A Method of ForCES Virtualization

Rong Jin,Xiongxiong He,Ming Gao 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.1

O-GlcNAc transferase regulates intervertebral disc degeneration by targeting FAM134B-mediated ER-phagy

Luo Rongjin,Li Gaocai,Zhang Weifei,Liang Huaizhen,Lu Saideng,Cheung Jason Pui Yin,Zhang Teng,Tu Ji,Liu Hui,Liao Zhiwei,Ke Wencan,Wang Bingjin,Song Yu,Yang Cao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Both O-linked β-N-acetylglucosaminylation (O-GlcNAcylation) and endoplasmic reticulum-phagy (ER-phagy) are well-characterized conserved adaptive regulatory mechanisms that maintain cellular homeostasis and function in response to various stress conditions. Abnormalities in O-GlcNAcylation and ER-phagy have been documented in a wide variety of human pathologies. However, whether O-GlcNAcylation or ER-phagy is involved in the pathogenesis of intervertebral disc degeneration (IDD) is largely unknown. In this study, we investigated the function of O-GlcNAcylation and ER-phagy and the related underlying mechanisms in IDD. We found that the expression profiles of O-GlcNAcylation and O-GlcNAc transferase (OGT) were notably increased in degenerated NP tissues and nutrient-deprived nucleus pulposus (NP) cells. By modulating the O-GlcNAc level through genetic manipulation and specific pharmacological intervention, we revealed that increasing O-GlcNAcylation abundance substantially enhanced cell function and facilitated cell survival under nutrient deprivation (ND) conditions. Moreover, FAM134B-mediated ER-phagy activation was regulated by O-GlcNAcylation, and suppression of ER-phagy by FAM134B knockdown considerably counteracted the protective effects of amplified O-GlcNAcylation. Mechanistically, FAM134B was determined to be a potential target of OGT, and O-GlcNAcylation of FAM134B notably reduced FAM134B ubiquitination-mediated degradation. Correspondingly, the protection conferred by modulating O-GlcNAcylation homeostasis was verified in a rat IDD model. Our data demonstrated that OGT directly associates with and stabilizes FAM134B and subsequently enhances FAM134B-mediated ER-phagy to enhance the adaptive capability of cells in response to nutrient deficiency. These findings may provide a new option for O-GlcNAcylation-based therapeutics in IDD prevention.

Erratum to "Relationships between Teaching Professional Rank, Course Taking, Teaching Experience and Knowledge of Algebra for Teaching"

Huang, Rongjin Korean Society of Mathematical Education 2014 수학교육연구 Vol.18 No.3

In situ monitoring of pore structure of magnesium oxysulfate cement paste: Effect of MgSO4/H2O ratio

Shengwen Tang,Changrong Wei,Rongjin Cai,Jiasheng Huang,E. Chen,Junhui Yuan 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.83 No.-

This work systematically presented the pore structure evolution of different magnesium oxysulfate(MOS) cement pastes by non-contact impedance measurement (NCIM), fractal theory and othermicrostructural experiments. According to the evolution of electrical impedance response, threehydration stages (dissolution, acceleration and hardened stages) can be identified in the pastes. Furthermore, based on the results obtained from NCIM and fractal theory, the porosity, incremental porevolume, pore tortuosity, average pore diameter and maximal pore diameter of MOS cement pastes areinvestigated. The influence of MgSO4/H2O ratio on pore structure of MOS cement paste is primarilyexplained. It can be inferred from predicted and experimental results that NCIM is a good method toanalyze the microstructure evolution of MOS cement pastes in-situ.

Cytosolic escape of mitochondrial DNA triggers cGAS-STING-NLRP3 axis-dependent nucleus pulposus cell pyroptosis

Zhang Weifeng,Li Gaocai,Luo Rongjin,Lei Jie,Song Yu,Wang Bingjin,Ma Liang,Liao Zhiwei,Ke Wencan,Liu Hui,Hua Wenbin,Zhao Kangcheng,Feng Xiaobo,Wu Xinghuo,Zhang Yukun,Wang Kun,Yang Cao 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Low back pain (LBP) is a major musculoskeletal disorder and the socioeconomic problem with a high prevalence that mainly involves intervertebral disc (IVD) degeneration, characterized by progressive nucleus pulposus (NP) cell death and the development of an inflammatory microenvironment in NP tissue. Excessively accumulated cytosolic DNA acts as a damage-associated molecular pattern (DAMP) that is monitored by the cGAS-STING axis to trigger the immune response in many degenerative diseases. NLRP3 inflammasome-dependent pyroptosis is a type of inflammatory programmed death that promotes a chronic inflammatory response and tissue degeneration. However, the relationship between the cGAS-STING axis and NLRP3 inflammasome-induced pyroptosis in the pathogenesis of IVD degeneration remains unclear. Here, we used magnetic resonance imaging (MRI) and histopathology to demonstrate that cGAS, STING, and NLRP3 are associated with the degree of IVD degeneration. Oxidative stress induced cGAS-STING axis activation and NLRP3 inflammasome-mediated pyroptosis in a STING-dependent manner in human NP cells. Interestingly, the canonical morphological and functional characteristics of mitochondrial permeability transition pore (mPTP) opening with the cytosolic escape of mitochondrial DNA (mtDNA) were observed in human NP cells under oxidative stress. Furthermore, the administration of a specific pharmacological inhibitor of mPTP and self-mtDNA cytosolic leakage effectively reduced NLRP3 inflammasome-mediated pyroptotic NP cell death and microenvironmental inflammation in vitro and degenerative progression in a rat disc needle puncture model. Collectively, these data highlight the critical roles of the cGAS-STING-NLRP3 axis and pyroptosis in the progression of IVD degeneration and provide promising therapeutic approaches for discogenic LBP.

The technology and properties of sponge city permeable bricks prepared using refractory waste

Jieguang Song,Xueqing Yang,Ping Chen,Rongjin Liu,Deping Luo,Yuxuan Wei,Wenjin Yao,Jingjing Liu,Qing Zeng 한양대학교 세라믹연구소 2021 Journal of Ceramic Processing Research Vol.22 No.4

With the widespread development of sponge city projects across the country, the use of industrial permeable bricks hasincreased dramatically. My country produces a large amount of refractory waste and pollutes the environment seriously. Wasterefractory insulation bricks are used as the main raw materials, and a small amount of auxiliary raw materials are added toprepare sponge urban permeable bricks through forming and sintering processes. Through performance tests, such asporosity, flexural strength, and water permeability coefficient, the effect of process parameters on the performance ofpermeable bricks is studied, the process parameters of using refractory waste are comprehensively optimized to preparesponge urban permeable bricks, and permeable bricks are prepared for the industrialization of high-quality industrial solidwaste. Brick provides reference. Through the experimental results and analysis, the following conclusions are drawn: the rawmaterial ratio of refractory brick waste: binder: foaming agent: sintering aid is 80:10:1:9. Slurry water mill foaming occurswhen the water to material ratio is 0.5, the ball milling speed is 80 r/min, and the ball milling time is 2 h; the foaming effectand molding performance are better. In the drying stage, drying at 45 oC for 12 h has the best molding effect. The sinteringsystem at 1,300 oC and holding time of 1 h has the best sintering performance. After optimizing the process, the porosity ofthe permeable bricks can reach 48.4%, the water permeability coefficient is 2.1×10^-2 cm/s, the national permeable brick hasA-level standard, and the compressive strength is 26.8 MPa.

Mitochondrial quality control in intervertebral disc degeneration

Yu Song,Saideng Lu,Wen Geng,Xiaobo Feng,Rongjin Luo,Gaocai Li,Cao Yang 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Intervertebral disc degeneration (IDD) is a common and early-onset pathogenesis in the human lifespan that can increase the risk of low back pain. More clarification of the molecular mechanisms associated with the onset and progression of IDD is likely to help establish novel preventive and therapeutic strategies. Recently, mitochondria have been increasingly recognized as participants in regulating glycolytic metabolism, which has historically been regarded as the main metabolic pathway in intervertebral discs due to their avascular properties. Indeed, mitochondrial structural and functional disruption has been observed in degenerated nucleus pulposus (NP) cells and intervertebral discs. Multilevel and well-orchestrated strategies, namely, mitochondrial quality control (MQC), are involved in the maintenance of mitochondrial integrity, mitochondrial proteostasis, the mitochondrial antioxidant system, mitochondrial dynamics, mitophagy, and mitochondrial biogenesis. Here, we address the key evidence and current knowledge of the role of mitochondrial function in the IDD process and consider how MQC strategies contribute to the protective and detrimental properties of mitochondria in NP cell function. The relevant potential therapeutic treatments targeting MQC for IDD intervention are also summarized. Further clarification of the functional and synergistic mechanisms among MQC mechanisms may provide useful clues for use in developing novel IDD treatments.

Influence of raw materials ratio on performance of permeable bricks made from electric porcelain waste

Jieguang Song,Xueqing Yang,Ping Chen,Hao Xu,Deping Luo,Rongjin Liu,Zhijun Lai 한양대학교 세라믹연구소 2022 Journal of Ceramic Processing Research Vol.23 No.4

The prepared permeable bricks using electric porcelain waste as the main raw material are investigated, which improves theutilization rate of electric porcelain waste, and realizes the organic unity of recycling resource and ecological environmentprotection. The effects of different raw material ratios on the permeability coefficient, apparent porosity, compressive strength,volume shrinkage rate and microstructure properties of electric porcelain waste permeable bricks are investigated. The electricporcelain waste with a ratio of over 85 wt.% have greatly improved the utilization rate of electric porcelain waste. The resultsare shown that the water-to-material ratio or foaming agent content are increased, the porosity and water permeability of theelectric porcelain waste permeable brick are increased, but the compressive strength of the electric porcelain waste permeablebrick is decreased. The 90 wt.% electric porcelain waste, the 3 wt.% kaolin, the 5 wt.% sintering aid, and the 2 wt.% foamingagents are optimum raw materials ratios, the water-to-material ratio for 0.9 and the drying temperature at 40 oC, the sinteringtemperature at 1200 oC and holding time for 1 h are obtained the excellent performance electric porcelain waste permeablebricks. The permeability coefficient is 3.9×10−2 cm/s, the volume shrinkage rate is 37.88%, the compressive strength is 1.146MPa, and the porosity is 79.0%.

다차원 영상을 활용한 매개모기 자동탐지 장치 개발

이상준 ( Sang-joon Lee ),김한기 ( Han-gi Kim ),조병관 ( Byoung-kwan Cho ),이희일 ( Hee-il Lee ),조선란 ( Sun-ran Cho ),김기훈 ( Gi-hun Kim ),정롱진 ( Rongjin Jung ),유동식 ( Dongsik Ryu ) 한국농업기계학회 2022 한국농업기계학회 학술발표논문집 Vol.27 No.2

세계보건기구(WHO)에 의하면 말라리아, 일본 뇌염 그리고 뎅기 바이러스 등 모기 매개 감염병으로 인해 매년 70만명 이상이 사망한다. 최근 이상기온 및 기상이변으로 모기 개체수가 증가하여 감염병에 대한 노출이 심화되는 추세이다. 그러나 말라리아 이외의 모기 매개 감염병은 치료제가 개발되어 있지 않으므로 사전 방역 및 방제를 통한 매개모기 감염병에 대한 노출을 최소화하는 것이 중요하다. 효과적인 매개모기 감염병 확대 방지를 위해서는 감염병을 매개하는 모기 종류에 대한 개체수 모니터링이 필수적이다. 하지만 현재 모기 포집 장치는 종이 아닌 개체수만 측정이 가능하여 종별 감염병에 대해 효과적인 방제 체계에 기여를 하지 못하고 있다. 따라서, 본 연구에서는 딥러닝 영상분석기술을 활용하여 국내 주요 매개 모기 5종에 대해 종 동정 및 개체수 파악이 가능한 매개 모기 자동 탐지 장치를 개발하였다. 모기 자동 탐지 장치는 전기 충격 방식과 배경판 슬라이딩 방식으로 포집된 모기의 컬러 및 형광 영상을 선명하게 촬영할 수 있도록 개발되었다. 실제 모기 포집 현장에서의 조건과 동일한 영상 데이터를 확보하기 위해 살아있는 13종의 야생 모기로 컬러 및 형광 이미지 데이터 셋을 구축하여 모기의 색, 형태 및 형광 특징을 분석에 활용할 수 있도록 하였다. 딥러닝 영상분석 알고리즘으로는 최신 BackBone인 Swin transformer와 딥러닝 객체 탐지 모델의 detector인 Faster R-CNN(Region-Convolutional Neural Network)을 결합하여 최종 탐지 모델을 구축하였다. 모델 성능 향상을 위해 컬러 및 형광 이미지의 예측 결과를 통합 후, NMS(Non-maximum suppression) 기법을 적용하였다. 외부 환경에 설치된 장치에 야생 모기를 투입하여 Blind test를 실시한 결과, 평균 91.7%의 분류 정확도를 확인할 수 있었다. 본 연구에서 개발된 딥러닝 객체 탐지 기술을 활용한 모기 포집 장치는 실외 현장에서 모기 종류 및 개체수 모니터링에 활용될 수 있을 것으로 사료된다.

다차원 영상을 활용한 매개모기 자동탐지 장치 개발

이상준 ( Sang-joon Lee ),김한기 ( Han-gi Kim ),조병관 ( Byoung-kwan Cho ),이희일 ( Hee-il Lee ),조선란 ( Sun-ran Cho ),김기훈 ( Gi-hun Kim ),정롱진 ( Rongjin Jung ),유동식 ( Dongsik Ryu ) 한국농업기계학회 2022 한국농업기계학회 학술발표논문집 Vol.27 No.2

세계보건기구(WHO)에 의하면 말라리아, 일본 뇌염 그리고 뎅기 바이러스 등 모기 매개 감염병으로 인해 매년 70만명 이상이 사망한다. 최근 이상기온 및 기상이변으로 모기 개체수가 증가하여 감염병에 대한 노출이 심화되는 추세이다. 그러나 말라리아 이외의 모기 매개 감염병은 치료제가 개발되어 있지 않으므로 사전 방역 및 방제를 통한 매개모기 감염병에 대한 노출을 최소화하는 것이 중요하다. 효과적인 매개모기 감염병 확대 방지를 위해서는 감염병을 매개하는 모기 종류에 대한 개체수 모니터링이 필수적이다. 하지만 현재 모기 포집 장치는 종이 아닌 개체수만 측정이 가능하여 종별 감염병에 대해 효과적인 방제 체계에 기여를 하지 못하고 있다. 따라서, 본 연구에서는 딥러닝 영상분석기술을 활용하여 국내 주요 매개 모기 5종에 대해 종 동정 및 개체수 파악이 가능한 매개 모기 자동 탐지 장치를 개발하였다. 모기 자동 탐지 장치는 전기 충격 방식과 배경판 슬라이딩 방식으로 포집된 모기의 컬러 및 형광 영상을 선명하게 촬영할 수 있도록 개발되었다. 실제 모기 포집 현장에서의 조건과 동일한 영상 데이터를 확보하기 위해 살아있는 13종의 야생 모기로 컬러 및 형광 이미지 데이터 셋을 구축하여 모기의 색, 형태 및 형광 특징을 분석에 활용할 수 있도록 하였다. 딥러닝 영상분석 알고리즘으로는 최신 BackBone인 Swin transformer와 딥러닝 객체 탐지 모델의 detector인 Faster R-CNN(Region-Convolutional Neural Network)을 결합하여 최종 탐지 모델을 구축하였다. 모델 성능 향상을 위해 컬러 및 형광 이미지의 예측 결과를 통합 후, NMS(Non-maximum suppression) 기법을 적용하였다. 외부 환경에 설치된 장치에 야생 모기를 투입하여 Blind test를 실시한 결과, 평균 91.7%의 분류 정확도를 확인할 수 있었다. 본 연구에서 개발된 딥러닝 객체 탐지 기술을 활용한 모기 포집 장치는 실외 현장에서 모기 종류 및 개체수 모니터링에 활용될 수 있을 것으로 사료된다.