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Functional Characterization of the Genes tauO, tauK, and tauI in the Biosynthesis of Tautomycetin
Fen Wang,Rixiang Kong,Bo Liu,Jing Zhao,Rongguo Qiu,Li Tang 한국미생물학회 2012 The journal of microbiology Vol.50 No.5
Tautomycetin is a specific protein phosphatase I inhibitor. In an effort to elucidate the biosynthetic mechanism of tautomycetin,we inactivated genes of the tautomycetin biosynthetic gene cluster, tauI, tauO, and tauK, which encode for putative P450 oxidase, citryl-CoA lyase, and esterase enzymes,respectively. The mutant STQ0606 (ΔtauO) did not produce any detectable amount of tautomycetin intermediates but could convert dialkylmaleic anhydride to tautomycetin,strongly indicating that TauO was involved in dialkylmaleic anhydride biosynthesis. STQ1211 (ΔtauK) accumulated dialkylmaleic anhydride, whereas the cofermentation of STQ1211(ΔtauK) and STQ0606 (ΔtauO) restored the production of tautomycetin. Together, these results suggest that TauK was responsible for the conjugation of dialkylmaleic anhydride and the polyketide moiety in tautomycetin biosynthesis. The disruption of tauI resulted in the accumulation of 5-des-ketotautomycetin,revealing that TauI was responsible for the oxidation at C5 as the last step. Although the shunt pathways were involved in the biosynthesis of tautomycetin, the main post-polyketide synthase tailoring steps were dehydration,decarboxylation and oxidation, taking place consecutively. This study allowed us to predict the biosynthesis of tautomycetin more accurately and provided novel insights into the mechanism of the biosynthesis of tautomycetin.
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Characteristics and model for growth of Rhizopus oryzae on the simulated gas-solid interface
shiru Jia,김선일,권규혁,조기안,Rixiang Kong,Huijun Dong,최두복 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.4
In order to investigate the effect on morphology of Rhizopus oryzae and production of lactic acid, various interface materials were used. Morphology of fungal showed sheet and flock when resin was added. The production of lactic acid was increased dramatically when interface materials were added. Furthermore, the effect of resin was more significant than that of others. It was assumed that interface materials could absorb substrate and microorganism together, so microorganism was not inhibited by substrate. The effect of static electric field on the interface culture was studied. When the exerting potential was 6.78 voltage, the biomass y was obviously higher than that of zero voltage. A simulated gas-solid interface system was developed to study the growth and two phases model for the growth of Rhizopus oryzae was build up that depended on the symmetric branching theory. An important parameter F was researched. The results indicated that the value of F had obvious difference at exponential and deceleration period, respectively.
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