Decolorization and Degradation of Reactive Azo Dyes by Fixed Bed Bioreactors Containing Immobilized Cells of Proteus vulgaris NCIM- 2027

Rijuta G. Saratale,Ganesh D. Saratale,Jo Shu Chang,Sanjay P. Govindwar 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.4

Immobilized cells of Proteus vulgaris NCIM 2027 completely decolorized C.I. Reactive Blue 172 (50mg/L) within 8 h along with a nearly 80% reduction in TOC and COD. The dye degradation efficiency of the immobilized cells was further improved by optimizing the physicochemical conditions, including agitation, temperature,pH, dye concentration, and biomass loading. Microbial toxicity study revealed the non-toxic nature of the degraded products. Repeated-batch decolorization was conducted to evaluate the reusability of the immobilized cells. The immobilized cells were used for continuous dye decolorization in a fixed bed bioreactor under different volumetric flow rates and dye feeding concentrations. In addition, the immobilized cells were applied to decolorize a mixture of seven reactive dyes in batch and continuous modes, resulting in efficient decolorization (in terms of ADMI value) and significant reduction in TOC and COD. This suggests the potential of using immobilized cells to treat dye-containing wastewater.

Influence of parameters on the photocatalytic degradation of phenolic contaminants in wastewater using TiO2/UV system

Saratale, Rijuta G.,Noh, Hyun S.,Song, Ji Y.,Kim, Dong S. Taylor Francis 2014 Journal of Environmental Science and Health. Part Vol.49 No.13

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Saratale, Rijuta Ganesh,Shin, Han Seung,Ghodake, Gajanan S.,Kumar, Gopalakrishnan,Oh, Min Kyu,Saratale, Ganesh Dattatraya Elsevier 2018 Bioresource technology Vol.258 No.-

<P><B>Abstract</B></P> <P>This study focuses on development of calcium peroxide (CaO<SUB>2</SUB>) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO<SUB>4</SUB>) and cobalt chloride (COCl<SUB>2</SUB>) in CaO<SUB>2</SUB> pretreatment resulted in maximum delignification of KCP relative to individual CaO<SUB>2</SUB> pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain <I>Klebsiella pneumoniae</I> KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed CaO<SUB>2</SUB> pretreatment & optimized various operational parameters for KCP biomass. </LI> <LI> Addition of inorganic salts improved delignification, glucose yield and total sugar recovery. </LI> <LI> Maximum BDO production (10.42 g/L) was achieved using <I>Klebsiella pneumoniae</I> KMK05. </LI> <LI> This novel pretreatment strategy abstained loss of sugar components of biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Electrochemical Oxidation of Phenol for Wastewater Treatment Using Ti/PbO2 Electrode

Saratale, Rijuta Ganesh,Hwang, Kyoung-Jin,Song, Ji-Young,Saratale, Ganesh Dattatray,Kim, Dong-Su American Society of Civil Engineers 2016 Journal of environmental engineering Vol.142 No.2

<P>The electrochemical oxidation of phenol was studied using a Ti/PbO2 electrode prepared by the electrodeposition method with PbO2 coated on Ti. The structural and morphological activity of Ti/PbO2 was analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD), and intermediates formed after degradation of phenol were quantitatively assessed by high-pressure liquid chromatography (HPLC). Optimization of various parameters such as current density, initial phenol concentration, initial solution pH, and different temperature and dose of Fe2+ on electrochemical degradation of phenol using Ti/PbO2 were investigated. Complete removal of phenol (250mgL-1) was observed at 50 degrees C, potential difference (5V), and at pH 2. Experimental results showed that the phenol removal rate increased with increasing current intensity along with significant reduction in total organic carbon (TOC). Fundamental kinetic data obtained for the degradation of phenol by Ti/PbO2 was found to follow in accordance with the zero-order kinetics with respect to the phenol concentration. This paper is expected to be useful for the development of electrochemical process using Ti/PbO2 for the degradation of phenol containing wastewater. (C) 2015 American Society of Civil Engineers.</P>

Anti-diabetic Potential of Silver Nanoparticles Synthesized with Argyreia nervosa Leaf Extract High Synergistic Antibacterial Activity with Standard Antibiotics Against Foodborne Bacteria

Saratale, Ganesh Dattatraya,Saratale, Rijuta Ganesh,Benelli, Giovanni,Kumar, Gopalakrishnan,Pugazhendhi, Arivalagan,Kim, Dong-Su,Shin, Han-Seung Springer-Verlag 2017 Journal of cluster science Vol.28 No.3

<P>The current investigation highlighted a novel cost-effective green synthesis of silver nanoparticles (AgNPs) using Argyreia nervosa leaves extract (ANE) as a potential reducing and capping agent. Surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at lambda (max) = 435 nm. FTIR revealed the involvement of biological macromolecules of ANE in the synthesis and stabilization of AgNPs. HRTEM images showed that the size of the spherical AgNPs ranged between 5 and 40 nm with average particle size of about 15 nm. The ANE-AgNPs showed inhibition activity against carbohydrate digestive enzymes alpha-amylase and alpha-glucosidase, with EC50 of 55.5 and 51.7 A mu g/mL, respectively, indicating its antidiabetic potential. The in vitro antioxidant activity of ANE-AgNPs was evaluated in terms of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals scavenging assays with IC50 value of 44.3 and 55.9 A mu g/mL, respectively. The AgNPs displayed strong antibacterial activity against foodborne bacteria with zone of inhibition 16.0 and 12.5 mm for Escherichia coli and Staphylococcus aureus, respectively, and also exhibited strong synergistic antibacterial activity together with standard antibiotics. The biological activity in terms of antioxidant, antidiabetic and antibacterial potential could be useful in various bio-applications such as cosmetics, food, and biomedical industry.</P>

Adsorptive remediation of cobalt oxide nanoparticles by magnetized α-cellulose fibers from waste paper biomass

Kadam, Avinash,Saratale, Rijuta Ganesh,Shinde, Surendra,Yang, Jiwook,Hwang, Kyojung,Mistry, Bhupendra,Saratale, Ganesh Dattatraya,Lone, Saifullah,Kim, Dae-Youg,Sung, Jung-Suk,Ghodake, Gajanan Elsevier 2019 Bioresource technology Vol.273 No.-

<P><B>Abstract</B></P> <P>Remediation of engineered-nanomaterials is an up-coming major environmental concern. This study demonstrates adsorptive-remediation of cobalt oxide nanoparticles (CoO NPs) from the water. The α-cellulose-fibers were extracted from waste-paper biomass (WP-αCFs) and magnetized with Fe<SUB>3</SUB>O<SUB>4</SUB> NPs (M-WP-αCFs). The XRD, FT-IR, and TGA were performed for detailed characterization of the newly developed bioadsorbent. The M-WP-αCFs was then applied for adsorptive remediation of CoO NPs. The adsorptive kinetics of CoO NPs adsorption onto the M-WP-αCFs reveals the pseudo-second-order model. The various adsorption isotherm studies revealed Langmuir is a best-fit isotherm. A prominently high adsorption capacity <I>q<SUB>m</SUB> </I> (1567 mg/g) corroborated extraordinary adsorptive potential of M-WP-αCFs. Furthermore, CoO NPs were adsorbed onto M-WP-αCFs were analyzed by the XPS, VSM, and TEM. Therefore, this study gave rise WP biomass extracted and rapidly-separable nano-biocomposite of ‘M-WP-αCFs’ with a high-capacity for CoO NPs remediation and can be further applied in remediation of several other engineered-nanomaterials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Office paper waste (WP) extracted α-cellulose fibers (αCFs) was magnetized. </LI> <LI> M-WP-αCFs presented an effective adsorptive-remediation of CoO NPs from the water. </LI> <LI> The adsorption kinetics followed a pseudo-second-order rate model. </LI> <LI> Langmuir adsorption isotherm model was best fit to the experimental data. </LI> <LI> M-WP-αCFs can be effective for remediation of engineered NPs from the water. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Screening and optimization of pretreatments in the preparation of sugarcane bagasse feedstock for biohydrogen production and process optimization

Saratale, Ganesh Dattatray,Saratale, Rijuta Ganesh,Kim, Sang Hyoun,Kumar, Gopalakrishnan Elsevier 2018 International journal of hydrogen energy Vol.43 No.25

<P><B>Abstract</B></P> <P>This work evaluated the effects of individual alkaline, sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB> denoted as; NaC), sodium sulfide (Na<SUB>2</SUB>SO<SUB>3</SUB> denoted as; NaS) and combination of NaC + NaS pretreatment for the saccharification of sugarcane bagasse (SCB). The effects of different pretreatments on chemical composition and structural complexity of SCB in relation with its saccharification were investigated. For enzymatic hydrolysis of pretreated SCB we have utilized the produced crude enzymes by <I>Streptomyces</I> sp. MDS to make the process more cost effective. A enzyme dose of 30 filter paperase (FPU) produced a maximum reducing sugar (RS) 592 mg/g with 80.2% hydrolysis yield from NaC + NaS pretreated SCB under optimized conditions. The resulted enzymatic hydrolysates of each pretreated SCB were applied for hydrogen production using <I>Clostridium beijerinckii</I> KCTC1785. NaC + NaS pretreated SCB hydrolysates exhibited maximum H<SUB>2</SUB> production relative to other pretreatment methods. Effects of temperature, initial pH of culture media and increasing NaC + NaS pretreated SCB enzymatic hydrolysates concentration (2.5–15 g/L) on bioH<SUB>2</SUB> production were investigated. Under the optimized conditions, the cumulative H<SUB>2</SUB> production, H<SUB>2</SUB> production rate, and H<SUB>2</SUB> yield were 1485 mL/L, 61.87 mL/L/h and 1.24 mmol H<SUB>2</SUB>/mol of RS (0.733 mmol H<SUB>2</SUB>/g of SCB), respectively. The efficient conversion of the SCB hydrolysate to H<SUB>2</SUB> without detoxification proves the viability of process for cost-effective hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatments of alkaline, sodium carbonate, sodium sulfide and combination demonstrated for sugarcane baggase. </LI> <LI> Several crucial factors towards optimization were carried out. </LI> <LI> Peak 59.2 g/L of reducing sugar (RS) with 80.2% hydrolysis yield from NaC + NaS pretreated SCB. </LI> <LI> Significant hydrogen production and process optimization using SCB hydrolysates studied in detail. </LI> </UL> </P>

Gallic acid-functionalized silver nanoparticles as colorimetric and spectrophotometric probe for detection of Al3+ in aqueous medium

Gajanan Ghodake,Surendra Shinde,Avinash Kadam,Rijuta Ganesh Saratale,Ganesh Dattatraya Saratale,Asad Syed,Omar Shair,Marzouq Alsaedi,Dae-Young Kim 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

Metal chelation-enhanced changes in surface plasmon resonance (SPR) band of gallic acid-functionalizedAgNP solution to monitor aluminum ions (Al3+) is reported herein. In aqueous solution, Al3+ selectivelyinduced a strong absorbance with large shifts up to125 nm from the SPR band with color changing fromorange to red. This study illustrates that hydroxyl groups of phenol affect the response of a probe thattunes the absorbance maxima to a longer wavelength at 525 nm. Moreover, the rapid formation of aAgNP-Al3+ coordination complex was demonstrated by real-time monitoring, adsorptive removal, andenergy dispersive spectroscopy mapping.

Fermentative Hydrogen Production Using Sorghum Husk as a Biomass Feedstock and Process Optimization

Ganesh D. Saratale,Siddheshwar D. Kshirsagar,Rijuta G. Saratale,Sanjay P. Govindwar,오민규 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

The potential of isolated actinomycetes and fungi were evaluated for the cellulase and xylanase production under solid state fermentation conditions. Maximal secretion of enzymes was observed with Phanerochaete chrysosporium using soybean straw. The potential of the produced crude enzyme complex was demonstrated by two-step enzymatic hydrolysis of untreated and mild acidpretreated sorghum husk (SH). A cellulase dose of 10 filter paper units (FPU) released 563.21 mg of reducing sugar (RS) per gram of SH with 84.45% hydrolysis and 53.64% glucose yields, respectively. Finally, enzymatic hydrolysates of SH were utilized for hydrogen production by Clostridium beijerinckii. Effects of temperature, pH of media, and substrate concentration on the biohydrogen production from SH hydrolysates were investigated. The optimal conditions for maximal hydrogen production using SH hydrolysate were determined to be a loading of 5.0 g RS/L, at 35°C, and controlled pH at 5.5. Under these optimal conditions, the cumulative H2 production, H2 production rate, and H2 yield were 1,117 mL/L, 46.54 mL/L/h, and 1.051 mol/mol RS, respectively. These results demonstrated a cost-effective hydrogen production is possible with sorghum husk as a lignocellulosic feedstock.

Water Purification Filter Prepared by Layer-by-layer Assembly of Paper Filter and Polypropylene-polyethylene Woven Fabrics Decorated with Silver Nanoparticles

Gajanan Ghodake,Surendra Shinde,Ganesh Dattatraya Saratale,Avinash Kadam,Rijuta Ganesh Saratale,Dae-Young Kim 한국섬유공학회 2020 Fibers and polymers Vol.21 No.4

Cellulose-based water filters are an affordable alternative to remove particulate matter; however, bacteria are toosmall to be removed simply through size exclusion. Cellulose-based water filters prepared by layer-by-layer (LBL) assemblywith polypropylene-polyethylene (PP/PE) fabric decorated with silver nanoparticles (AgNPs) were tested to remove bacteriafrom water samples. The gallic acid reduction method was used to produce potent antibacterial AgNPs; their decoration ontoPP/PE woven fabrics and the preparation of five-layered paper filters were further investigated. The use of acidic conditionsfor loading AgNPs and improving their spatial distribution onto the PP/PE fabrics, as revealed by scanning electronmicroscopy, was found to be correlated with the fabrics’ antibacterial activity. The PP/PE fabrics decorated with a higherdensity of AgNPs (at pH 2) showed 96.7 % and 97.9 % reductions in the growth of E. coli and S. aureus, respectively. Similarly, paper filters fabricated by LBL assembly of AgNP@PP/PE fabrics with cellulose filters deactivated growing E. coli and S. aureus bacteria with good efficiency: approximately 99.4 % and 98.7 %, respectively. The results indicate thatfabricating water purification filters from the cellulose-based paper is feasible with LBL type assembly. The assembled paperfilters could be commercialized for point-of-use water purification in the future to prevent the spread of water-borne diseases.