자궁적출술시 발견된 충수돌기 점액낭종

박은경 ( Eun Kyung Park ),서미영 ( Mi Young Seo ),팽기영 ( Ki Young Paeng ),임채춘 ( Chae Chun Rhim ),김도강 ( Do Kang Kim ),남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

Mucocele arised at the vermiform appendix is uncommon, either benign or malignancy and their clinical presentation is not specific. The preoperative diagnosis is rare,^1 their diagnosis is an incidental event. Mucocele seems to be developed due to chronic obstruction of the lumen of the appendix. Higa and Cowerkers^2 classified 73 cases of mucocele into three clinicopathologic entities; focal or diffuse mucosal hyperplasia, mucinous cystadenoma, mucinous cystadenocarcinoma. Appendiceal mucoceles are rare lesions of the appendix, characterized by a gross enlargement of the appendix from accumulation of mucoid substance within the lumen. It is encountered in only 01-04% of all appendectomies with a female predominance and an average age at the time of diagnosis over 50 years. A case of appendiceal mucocle found during total hysterectomy is presented with a brief review of the leteratures.

八物湯이 저산소증에 의한 배양심근세포고사에 미치는 영향

임은경(Eun-Kyung Rhim),신선호(Sun-Ho Shin) 대한한의학회 2004 대한한의학회지 Vol.25 No.2

Epidemiological comparison of three Myco­plasma pneumoniae pneumonia epidemics in a single hospital over 10 years

Kim, Eun-Kyung,Youn, You-Sook,Rhim, Jung-Woo,Shin, Myung-Seok,Kang, Jin-Han,Lee, Kyung-Yil The Korean Pediatric Society 2015 Clinical and Experimental Pediatrics (CEP) Vol.58 No.5

Purpose: Mycoplasma pneumoniae (MP) pneumonia epidemics have occurred in 3- to 4-year cycles in Korea. We evaluated the epidemiologic characteristics of MP pneumonia in Daejeon, Korea, from 2003 to 2012. Methods: We retrospectively analyzed 779 medical records of children (0-15 years of old) with MP pneumonia admitted to our institution and compared the data from 3 recent epidemics. Results: In 779 patients, the mean age and male-to-female ratio were $5.0{\pm}2.2$ years and 1:1, and most cases were observed in autumn. There were three epidemics during the study period, in 2003, 2006-2007, and 2011. In our comparison of the three epidemics, we found no differences in mean age, the male-to-female ratio, hospital stay, or the rate of seroconverters during hospitalization. All three epidemics began in early summer and peaked in September 2003 and 2011 and in October 2006 and then gradually decreased until the next year's spring season, although the 2006 epidemic extended further into 2007. The peak age groups in the children in 2003 and 2006 were 3-6 year-olds (57.5% and 56%, respectively), but in the 2011 epidemic, the peak group was 1-4 year-olds (46.5%). The proportion of the <2 years of age group was 20%, 15.7% and 28.8%, and >10 years of age group was 5.2%, 13.8%, and 14.8% of total patients, respectively. Conclusion: MP pneumonia outbreaks occurred every 3-4 years. The pattern of 3 recent epidemics was similar in demographic characteristics and seasonality with some variations in each outbreak.

Characteristics of coal upgraded with heavy oils

Eun Mi Jo,전동혁,In Su Park,김상도,Young-joon Rhim,Ho Kyung Choi,유지호,Jeong-HwanLim,Si Hyun Lee 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.6

We investigated the ability of an oil coating to upgrade Indonesian low-rank coal, which has a low ashcontent and a moisture content of approximately 30%. Proximate and ultimate analyses of the characteristics of coalsamples containing different amounts of asphalt (ASP) and palm fatty acid distillate (PFAD) were studied, includingthe samples’ calorific values, crossing-point temperatures (CPT), specific surface areas, pore sizes, structural changes,and moisture readsorption. The results showed that the 0.5% PFAD-coated coal was the highest quality. This coal showedfew physical and chemical changes, and it had a low surface area and a high CPT value.

Expression, Purification, and Characterization of Prothrombin Kringle 2

Rhim, Tai-Youn,Kim, Eun-kyung,Park, Chan-Soo,Kim, Soung-Soo Korean Society for Biochemistry and Molecular Biol 1999 Journal of biochemistry and molecular biology Vol.32 No.2

Previously, we reported that the prothrombin kringle 2 (fragment 2), induced by LPS administration into rabbit, inhibited bFGF-stimulated BCE cell growth (Lee et al., 1998). In this study, we cloned and overexpressed the kringle 2 domain of rabbit and human prothrombin as a fusion protein with the pelB leader sequence in E. coli using the T7 promoter. The fusion protein was cleaved during translocation into the peri plasmic space, and cleaved recombinant protein was readily isolated from whole cell lysate by DEAE-Sepharose and Sephacryl S-200 gel filtration chromatography. Both the recombinant rabbit and human prothrombin kringle 2 showed very similar biochemical and functional characteristics to the rabbit prothrombin kringle 2 purified from rabbit serum, in terms of abnormal electrophoretic migration and endothelial cell growth inhibitory activity.

Expression , Purification , and Characterization of Prothrombin Kringle 2

Kim, Eun Kyung,Kim, Soung Soo,Rhim, Tai Youn,Park, Chan Soo 생화학분자생물학회 1997 BMB Reports Vol.32 No.2

Previously, we reported that the prothrombin kringle 2 (fragment 2), induced by LPS administration into rabbit, inhibited bFGF-stimulated BCE cell growth (Lee et al., 1998). In this study, we cloned and overexpressed the kringle 2 domain of rabbit and human prothrombin as a fusion protein with the pelB leader sequence in E. coli using the T7 promoter. The fusion protein was cleaved during translocation into the periplasmic space, and cleaved recombinant protein was readily isolated from whole cell lysate by DEAE-Sepharose and Sephacryl S-200 gel filtration chromatography. Both the recombinant rabbit and human prothrombin kringle 2 showed very similar biochemical and functional characteristics to the rabbit prothrombin kringle 2 purified from rabbit serum, in terms of abnormal electrophoretic migration and endothelial cell growth inhibitory activity.

Quantitative biochemical characterization and biotechnological production of caspase modulator, XIAP: Therapeutic implications for apoptosis-associated diseases

Yun, Si-Eun,Nam, Min-Kyung,Rhim, Hyangshuk Elsevier 2018 Biochimica et biophysica acta, General subjects Vol.1862 No.7

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Regulating apoptosis is a common and essential therapeutic strategy for cancer and neurodegenerative disorders. Based on basic studies of apoptotic mechanisms, various researches have attempted to overcome the pathogenesis of such diseases by activating or inhibiting apoptosis. Generally, the biochemical characteristics of the target molecules should be evaluated along with understanding of their mechanisms of action during drug development. Among apoptotic regulators, XIAP serves as a potent negative regulator to block apoptosis through the inhibition of caspase (CASP)-9 and -3/7. Although XIAP is an attractive target with such apoptotic-modulating property, biochemical and biophysical studies of XIAP are still challenging.</P> <P><B>Methods</B></P> <P>In this study, the CASP-9 and -3/7 inhibitors XIAP, 242Δ and Δ230 were prepared using the pGEX expression system and biochemically characterized.</P> <P><B>Results</B></P> <P>These inhibitors were expressed in <I>Escherichia coli</I> at a concentration of ≥20 mg/L culture under a native condition with 0.01 mM IPTG induction. Notably, using a simple and rapid affinity purification technique, these CASP-9 and -3/7 inhibitors have been purified, yielding ≥5 mg/L culture at approximately 90% purity.</P> <P><B>Conclusions</B></P> <P>We have determined that HtrA2 specifically binds to the BIR2 and BIR3 of XIAP at a 1:1 molecular ratio. Moreover, in vitro cell-free CASP-9 and -3/7 activation-apoptosis assays have demonstrated that these purified XIAP proteins dramatically inhibit CASP-9 and -3/7 action.</P> <P><B>General significance</B></P> <P>Our system is suitable for biochemical studies, such as quantitation of the number of molecules acting on the apoptosis regulation, and provides a basis and insights that can be applied to the development of therapeutic agents for neurodegenerative disorders and cancer.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Development of a simple and rapid method for bacterial expression and purification of XIAP proteins, wild type, 242Δ, and Δ230 </LI> <LI> Structural verification of the purified XIAP proteins using GST pull-down with HtrA2 </LI> <LI> Functional verification of the purified XIAP proteins using cell-free caspase activation-apoptosis assays </LI> </UL> </P>

Effect of Drying Method on Antioxidant Activity of Jiwhang (Rehmannia glutinosa)

Jong-Whan Rhim,Yang Xi,Won-Chul Jeong,Kyung-Sik Ham,Ha-Sook Chung,Eun-Sil Kim 한국식품과학회 2009 Food Science and Biotechnology Vol.18 No.6

Jiwhang (Rehmannia glutinosa), one of the most widely used medicinal herbs, was dried with various methods such as sun drying, hot air drying, vacuum drying, and freeze drying methods, and their effects on the antioxidant capacity in relation with the content of total phenolic compounds were studied with a steamed-and-dried rehmannia (sookjiwhang) for comparison. Generally, total phenolic contents decreased significantly by all of the drying treatments except the steamed-anddried rehmannia, in which total phenolic contents increased 2.4 fold compared with fresh rehmannia. Content of verbascoside, a functional phenolic compound, was the highest in the freeze-dried rehmannia (177.97±0.02 ㎍/g d.m.) followed by vacuumdried (105.55±0.07 ㎍/g d.m.), hot air-dried (23.01±0.02 ㎍/g d.m.), and sun-dried (4.89±0.13 ㎍/g d.m.) ones comparable to the fresh rehmannia (80.15±1.26 ㎍/g d.m.). Antioxidant capacity determined by both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azinobis (3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) methods agreed with the result of total phenolic contents, that is, the antioxidant capacity was the highest in the steamed-and-dried rehmannia followed by fresh rehmannia, vacuumdried, hot air-dried, sun-dried, and freeze-dried ones. Conclusively, the total phenolic contents and antioxidant capacity of rehmannia were greatly affected by the drying methods used.

Outbreaks of mumps: an observational study over two decades in a single hospital in Korea

Ryu, Ji-Ung,Kim, Eun-Kyung,Youn, You-Sook,Rhim, Jung-Woo,Lee, Kyung-Yil The Korean Pediatric Society 2014 Clinical and Experimental Pediatrics (CEP) Vol.57 No.9

Purpose: The introduction of the mumps vaccine has dramatically reduced the number of mumps cases, but outbreaks have recently occurred among highly vaccinated populations in developed countries. Epidemiological and clinical characteristics of patients with mumps admitted between 1989 and 2012 in a single hospital in Korea are described in the present study. Methods: We retrospectively evaluated inpatients with mumps between 1989 and 2012 and outpatients and inpatients with mumps in 2011-2012. Results: A total of 152 patients with mumps were admitted between 1989 and 2012, and 163 patients were recorded in 2011-2012. The highest number of admitted cases occurred in 1998 and 2012 (35 and 34 cases, respectively). Among the patients admitted in 2011-2012, the highest frequency was observed among people aged 15-19 years, and low frequency was observed in those aged <4 years and >20 years, compatible to the city data and national data. In patients admitted to our department in 1998 (35 cases) and in 2010-2012 (27 cases), there were significant differences in the mean age and the rate of secondary measles-mumps-rubella (MMR) vaccination, but had similar clinical features, including complications, except aseptic meningitis. Antimumps immunoglobulin (Ig) G was positive in 83% and 100%, and IgM was positive in 67% and 41%, respectively, in the two periods. Conclusion: In Korea, recent mumps outbreaks have occurred mainly among secondary school students who received two doses of the MMR vaccine. The vaccinees might have a modified immune reaction to viral insults, manifesting modified epidemiological and clinical features.

산화적 손상에 의해 유발된 심근세포 독성에 대한 보양환오탕(補陽還五湯)의 방어효과

임은경,정현애,신선호,이윤재,Rhim, Eun-Kyung,Jeong, Hyun-Ae,Shin, Sun-Ho,Lee, Yun-Jae 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

The water extract of Boyanghwanoh-tang has been used for treatment of ischemic vascular disease in oriental medicine. However, little is known about the mechanism by which the water extract of Boyanghwanoh-tang rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of Boyanghwanoh-tang on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that, after treatment of H9c2 cells with zinc, there was a decrease in cell viability in a dose dependent manner, and there was a chromatin condensation. Zinc induced the change of cell morphology. In addition, zinc induced mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of Boyanghwanoh-tang consistently with increase of the peroxoredoxin 1, 2, 3, 5, and 6 expression. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells, and that protective effects of Boyanghwanoh-tang against oxidative injuries are achieved through regulation of peroxiredoin expression.