Experiments on the bearing capacity of tapered concrete filled double skin steel tubular (CFDST) stub columns

Qing-Xin Ren,Chao Hou,Dennis Lam,Lin-Hai Han 국제구조공학회 2014 Steel and Composite Structures, An International J Vol.17 No.5

Tapered concrete filled double skin steel tubular (CFDST) columns have been used in China for structures such as electricity transmission towers. In practice, the bearing capacity related to the connection details on the top of the column is not fully understood. In this paper, the experimental behaviour of tapered CFDST stub columns subjected to axial partial compression is reported, sixteen specimens with top endplate and ten specimens without top endplate were tested. The test parameters included: (1) tapered angle, (2) top endplate thickness, and (3) partial compression area ratio. Test results show that the tapered CFDST stub columns under axial partial compression behaved in a ductile manner. The axial partial compressive behaviour and the failure modes of the tapered CFDST stub columns were significantly influenced by the parameters investigated. Finally, a simple formula for predicting the cross-sectional capacity of the tapered CFDST sections under axial partial compression is proposed.

The Decomposition and Optimization of the Global Query of Heterogeneous Database System Based on Neural Network Technique

Ren-qing Li,Zhong-de Yu 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

To decompose and optimize a global query perfectly is very hard in the field of heterogeneous database integration. Although there are a lot of methods about the decomposition and optimization of global query, but very few reliable work is reported in this area. This paper describes a new method of decomposition and optimization for integral query of heterogeneous database based on neural network technique. This method can decompose a global query into subqueries promptly and optimize these subqueries effectively. Its feasibility has been verified in a simplified heterogeneous database system.

Tick-Borne Pathogens in Ixodid Ticks from Poyang Lake Region, Southeastern China

Wei Qing Zheng,Xue Nan Xuan,Ren Long Fu,Hui Ying Tao,Yang Qing Liu,Xiao Qing Liu,Dong Mei Li,Hong Mei Ma,Hai Ying Chen 대한기생충학열대의학회 2018 The Korean Journal of Parasitology Vol.56 No.6

Single Nucleotide Polymorphisms of the GnRHR Gene Associated with Reproductive Traits of Japanese Flounder (Paralichthys olivaceus)

He, Feng,Wen, Hai-Shen,Li, Ji-Fang,Yu, Da-Hui,Ma, Rui-Qin,Shi, Dan,Mu, Wei-Jie,Zhang, Yuan-Qing,Hu, Jian,Liu, Miao,Han, Wei-Guo,Zhang, Jia-Nan,Wang, Qing-Qing,Yuan, Yu-Ren,Liu, Qun Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.4

Gonadotropin-releasing hormone receptor (GnRHR) gene is expressed at the anterior pituitary gland and plays a key role in gonad development. This study aimed to investigate molecular genetic characteristics of the GnRHR gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of GnRHR gene on sex steroid level in Japanese flounder (Paralichthys olivaceus). We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the GnRHR gene in 75 individuals. We identified three SNPs in the GnRHR gene: P1 locus (C759A and C830T) in the coding region of exon2 which were both linked together and P2 locus (G984T) in the coding region of exon3, which added a new transcript factor (ADR1) and a new methylation site (CG). Only C830T of P1 leads to amino acid changes Thr266Ile. Statistical analysis showed that P1 was significantly associated with $17{\beta}$-estradiol ($E_2$) level (p<0.01) and gonadosomatic index (GSI) (p<0.05). Individuals with genotype BB of P1 had significantly higher serum $E_2$ levels (p<0.01) and GSI (p<0.05) than those of genotype AA or AB. Another SNP, P2, synonymous mutation, was significantly associated with GSI (p<0.05). Individuals with genotype AB of P2 had significantly higher GSI (p<0.05) than that of genotype AA. In addition, there was a significant association between one diplotype based on three SNPs and reproductive traits. The genetic effects for both serum $E_2$ level and GSI of diplotype D4 were super diplotypes (p<0.05). These results suggest that the SNPs in Japanese Flounder GnRHR are associated with $E_2$ level and GSI.

Determination of Highly Soluble L-Carnitine in Biological Samples by Reverse Phase High Performance Liquid Chromatography with Fluorescent Derivatization

Qing-Ri Cao,Shan Ren,Mi-Jin Park,Yun-Jaie Choi,Beom-Jin Lee 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.8

This study was performed in order to validate an effective high performance liquid chromatograpy (HPLC) method to determine L-carnitine in biological samples such as plasma, milk and muscle in cows. An L-carnitine derivative for fluorescence absorption was synthesized with 1-aminoanthracene (16 mg/mL in acetone) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC; 160 mg/mL in 0.01 M NaH2PO4 buffer) as a precolumn fluorescent derivative reagent. γ-Butyrobetaine HCl was used as an internal standard. A reversedphase column with fluorescence detection at the excitation and emission wavelengths of 248 and 418 nm were used. The mobile phase consisted of 30% acetonitrile with 0.1 M ammonium acetate in water (pH 3.5) adjusted with acetic acid and delivered at a flow rate of 1.5 mL/ min. The L-carnitine concentration in plasma, milk and muscle samples of cows after oral feeding with 24 g L-carnitine/day for 2 months was then determined. All biological samples were deproteinated by barium hydroxide and zinc sulfate heptahydrate before the derivative reaction. Blank cow plasma was dialyzed using cellulose membrane for standard calibration. The calibration curve showed good linearity (r2 >0.999) over the concentration range of 50 to 5000 ng/mL. The precision and accuracy were also satisfactory with less than 15% intra- and interday coefficiency of variations. The peaks of L-carnitine and internal standard in HPLC chromatography were successfully separated in plasma, milk and muscle samples of cows. The current derivatization method of L-carnitine for fluorescence detection was simple and adequately sensitive and could be applied to determine L-carnitine in biological samples.

Obstacle crossing and traction performance of active and passive screw pipeline robots

Qing Tu,Qingyou Liu,Tao Ren,Yujia Li 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.5

Pipelines are important tools for transporting fluids. The regular use of pipeline robots to inspect pipelines is an important safety measure. Passive and active screw pipeline robots have been developed and have various characteristics. In this work, the structure and driving method of screw pipeline robots were introduced. An obstacle crossing model of active and passive screw pipeline robots was established and compared using the developed screw pipeline robots. A traction model of the active and passive screw pipeline robots that considers lateral and longitudinal slip characteristics was obtained. Then, the proposed pipeline robot traction model with lateral and longitudinal slips was verified through experiments. A traction experiment on the active screw pipeline robot with improved contact confirmed that an increase in the adhesion coefficient and reduction in driving wheel slip can increase traction force.

Mesoporous CexZr1−xO2 solid solutions supported CuO nanocatalysts for toluene total oxidation

Qing-Fang Deng,Tie-Zhen Ren,Bao Agula,Yuping Liu,Zhong-Yong Yuan 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5

Mesoporous CexZr1 xO2 solid solutions were prepared by the surfactant-assisted method and used as support of CuO nanocatalysts for catalytic total oxidation of toluene. The prepared CuO/CexZr1 xO2 catalysts have a wormhole-like mesoporous structure with high surface area and uniform pore size distribution, and the CuO nanoparticles were highly dispersed on the surface of CexZr1 xO2. The doping of ZrO2 in CeO2 promotes the dispersion of active copper species and enhances the reducibility of copper species. The effect of Ce/Zr ratio, calcination temperature and CuO loading amount on the catalytic performance of CuO/CexZr1 xO2 was investigated in detail. The 400 ℃-calcined 8%CuO/Ce0.8Zr0.2O2 catalyst exhibits the highest activity with the complete toluene conversion temperature of 275 ℃ at the condition of GHSH = 33,000 h-1 and the toluene concentration of 4400 ppm. The interfacial interaction between CuO and the CexZr1 xO2 support, highly dispersed CuO nanoparticles and the nature of the support contribute to the high catalytic activity of mesoporous CuO/CexZr1 xO2 nanocatalysts.

Risk assessment of remanufacturing arm structure for crane based on potential failure mode

Qing Dong,Gening Xu,Huili Ren 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.12

For the risk of remanufacturing arm structure with multi-coupling defects and the correlation and influence between risks factors, thejib structure risk evaluation theory based on improved DEMATEL method (JSRET-IDM) is presented to build a risk assessment modelfor jib structure in accordance with potential failure modes and to develop a risk assessment system of remanufacturing jib structures formobile crane. Failure performance datum of jib structure in service being collected, researched, and analyzed, the mechanical property,notch-crack and welding performance are backdated under characteristic parameters and typical service conditions to set up the failuremodes database. Testing positions and points can be determined according to the database. The direct and indirect influence fuzzy relationmatrixes between testing points or evaluation indexes are established by IDM to deduce influence matrixes and affected matrixes ofevery evaluation index, aiming at obtaining the centrality and reason degree being used to determine weighting factor of each index basedupon making an analysis of the correlation and influence of the indexes. Accordingly, combined with FSET, the failure risk can be easilyquantified. Using it as a base, the risk system is developed to realize the real-time, accurate and rapid assessment of the failure risk for jibstructure. Taking QY130 remanufacturing arm structure as an example, the effectiveness and scientificity of the proposed method andsystem are verified.

Protease Properties of Protease-Producing Bacteria Isolated from the Digestive Tract of Octopus vulgaris

Qing Liu(류청),Pei Ren,Meizi Piao,Ji-young Yang(양지영) 한국생명과학회 2013 생명과학회지 Vol.23 No.12

Octopus vulgaris의 장관으로부터 단백질 가수분해력과 활성을 측정함으로서 높은 단백질분해효소 생성능을 가진 균을 분리하여 동정하였다. 균이 생성한 단백질분해효소는 황산암모늄침전, cellulose CM-52 양이온 교환 크로마토그래피, DEAE-Sephadex A50 음이온 교환 크로마토그래피의 3단계를 통해 정제하였다. 장관으로부터 분리한 균중 가장 높은 단백질분해효소 생성능을 가진 균은 Bacillus sp. QDV-3로 나타났으며 이균을 분리한 후 표현형 분석, 생화학적 특성, 16S rRNA 유전자염기서열분석을 통해 Bacteria역, Firmicutes문, Bacilli강, Bacillales목, Bacillaceae과, Bacillus속으로 Bacillus flexus와 99.2%의 유사성을 보이는 것으로 확인하였다. 균이 생성한 단백질 분해효소를 QDV-E로 지정하였으며 61.6 kDa의 분자량을 나타내었다. 이 효소는 pH 9.0~9.5에서 활성을 나타내었고 최적온도는 40℃였으며 50℃에서는 60분간 96% 이상의 활성을 보유하였다. Phenyl methyl sulfonyl fluoride(PMSF)에 의하여 활성이 억제 되었으므로 세린 알칼리성 단백 분해 효소인 것으로 결론지었다. 금속이온인 Mn<SUP>2+</SUP>와 Mg<SUP>2+</SUP>에 의하여 효소활성 상승효과를 보였으며 Ba<SUP>2+</SUP>, Zn<SUP>2+</SUP>, 그리고 Cu<SUP>2+</SUP>에 의하여 활성이 억제되었다. A high protease-producing strain was isolated and identified from the digestive tract of octopus vulgaris by detecting a hydrolysis circle of protease and its activity. The strain was identified by morphology observation, biochemical experiments, and 16S rRNA sequence analysis. The protease obtained from the strain was purified by a three-step process involving ammonium sulfate precipitation, carboxy methyl-cellulose (CM-52) cation-exchange chromatography, and DEAE-Sephadex A50 anion-exchange chromatography. The properties of protease were characterized as well. The strain Bacillus sp. QDV-3, which produced the highest activity of protease, was isolated. On the basis of the phenotypic and biochemical characterization and 16S rRNA gene-sequencing studies, the isolate was identified as follows: domain: Bacteria; phylum: Firmicutes; class: Bacilli; order: Bacillales; family: Bacillaceae; and genus: Bacillus. The isolate was shown to have a 99.2% similarity with Bacillus flexus. A high active protease designated as QDV-E, with a molecular weight of 61.6 kDa, was obtained. The enzyme was found to be active in the pH range of 9.0-9.5 and its optimum temperature was 40℃. The protease activity retained more than 96% at the temperature of 50℃ for 60 min. Phenylmethylsulfonyl fluoride (PMSF) inhibited the enzyme activity, thus confirming that this protease isolated from Bacillus sp. QDV-3 is an alkaline serine protease. Metal ions, Mn<SUP>2+</SUP> and Mg<SUP>2+</SUP>, were determined to enhance the protease activity, whereas Ba<SUP>2+</SUP>, Zn<SUP>2+</SUP>, and Cu<SUP>2+</SUP> were found to inactivate the enzyme.

Interaction and micellar behavior of ternary mixture of amphoteric amino sulfonate surfactant with traditional anionic and nonionic surfactants: Effect of hydrophilicity

Qing Hua Zhang,Rui Sheng,Zhao Hua Ren,Jing Huang,Yun Xiao Wang,Ben Ru Wang,Xi Lei Huang,Qin Cheng,Xue Mei Wu,Tong Bo Wang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-

The micellization and the molecular interaction behaviors for two ternary mixtures constituted by anamphoteric sodium 3-(n-dodecyl ethylenediamino)-2-hydroxypropyl sulfonate (C12AS), an anionicsodium dodecylbenzene sulfonate (SDBS) and a nonionic octylphenol polyoxyethylated ether with thenumber (n) of oxyethylene glycol ethers OP-n (n = 10 or 7) in aqueous solution were investigated usingthe tensiometry and the effect of hydrophilicity on them was also discussed. In the framework of pseudophaseseparation model and based on the regular solution theory, the related micellization parametersincluding the mixed critical micelle concentration (cmc) values in the ideal and real cases, the activitycoefficients and the compositions in mixed micelle, etc. and thermodynamic parameters were estimatedby the Clint’s model and the Rubingh’ model. The mixed cmc value is dependent on the composition inaqueous solution and influenced by the hydrophilicity of nonionic surfactant. With increasing the nonionicin ternary mixture, the mixed cmc value is initially decreased and then slightly rise. An increasingin the hydrophilicity of nonionic will make the minimum value of mixed cmc be increased from1.299 mM to 1.705 mM. The resulting phenomena can be explained reasonably by the electrostatic effect,the steric hindrance, the hydrogen bonding, etc. Thermodynamic data indicate that the contribution ofentropy or enthalpy plays a vital role on the spontaneous process of micellization and the share ofentropy or enthalpy in free energy change is dependent largely on the amount of nonionic surfactantand the hydrophilicity. In ternary mixtures of C12AS/SDBS/OP-10, an increase in an amount of OP-10 willinduce the change from the enthalpy-driven micellization process to the entropically favorable process. Once an abundant amount of OP-7 is added, while, the enthalpy will make a main contribution on themicellization process, which can be described by the drop in the share of entropy at the composition(0.6970/0.0000/0.3030) of ternary mixture from 0.6478 to 0.4901. These findings will help with understandingthe molecular interaction behavior for the ternary surfactant mixture and the effect of the additionof nonionic surfactant and its hydrophilicity.