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- 저자 : Ramos-Bueno (검색결과 2 건)
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Patricia Cincotto dos Santos Bueno,Sandra Maria Barbalho,Elen Landgraf Guiguer,Maricelma da Silva Soares de Souza,Isabela Ramos Arau´jo de Medeiros,Isabella Vasconcelos Zattiti,Manuela dos Santos Buen 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12
Studies have shown that the consumption of a diet containing whole grains may improve metabolic homeostasis and is related to the reduction of risk factors for the development of obesity, diabetes, cardiovascular diseases, and cancer. We aimed to investigate the effects of Triticum aestivum and Triticum turgidum on the metabolic profile of Wistar rats. Animals were divided into G1 (control group), G2 (T. turgidum), and G3 (T. aestivum). Anthropometric and biochemical parameters were evaluated after 45 days of treatment with both types of wheat. Our results showed that the use of the common or green wheat improved body weight percentage, visceral fat, glycemia, low-density lipoprotein cholesterol, triglycerides, and atherogenic indices such as atherogenic index, CCR1, and CCR2. Furthermore, wheat may also improve high-density lipoprotein cholesterol levels. The health-promoting properties of wheat occur probably due to the content of phytochemicals, antioxidants, and fibers. We suggest that the intake of T. aestivum and T. turgidum may be helpful in the prevention or treatment of obesity, diabetes and cardiovascular diseases.
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Cytotoxicity Screening of Several Tomato Extracts
Jose´ Luis Guil-Guerrero,Rebeca Ramos-Bueno,Ignacio Rodríguez-García,Cristóbal López-Sánchez 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.1
The cytotoxic effects of extracts of the tomato variety “Racimo” have been evaluated through the use of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at several concentrations. Three extracts—ethanol–water, petroleum ether, and in vitro digested tomato—exhibited in vitro cytotoxicity against the proliferation of the cultured cancer cell line HT-29. The concentration that caused 50% inhibition of cancer cell growth occurred (GI50) of the different extracts for HT-29 cells was 62.5μg/mL for the petroleum ether extract and 87.0μg/mL for the digested tomato extract. For the ethanol–water extract, it was not possible to determine this parameter at the assayed extract concentrations. These results clearly indicate that after the digestion process, the less polar substances, such as carotenoids and sterols, are bioavailable as active species against cancer cells. The GI50 levels for tomato extracts are similar to those values reported for medicinal plants. The results of the MTT assay on nonmutagenic CCD-18 cells showed a lack of negative effect on cell growth, which indicates that tomato extracts act selectively on HT-29 tumor cells. ^1H-Nuclear magnetic resonance spectra confirmed the presence of known compounds with accepted cytotoxic activity against tumor lines (lycopene and β-carotene). The high cytotoxicity for HT-29 cells showed by the petroleum ether extract might be due to the simultaneous presence in the extract of both carotenoids and glyceryl esters of fatty acids. The results of this work clearly indicate the importance of carotenoid consumption on colon tumor proliferation and prevention, and also the importance of the dietary fats in carotenoid bioavailability.
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