First Molecular Characterization of Hypoderma actaeon in Cattle and Red Deer (Cervus elaphus) in Portugal

Haroon Ahmed,Sérgio Ramalho Sousa,sami simsek,Sofia Anastácio,Seyma Gunyakti Kilinc 대한기생충학ㆍ열대의학회 2017 The Korean Journal of Parasitology Vol.55 No.6

Hypoderma spp. larvae cause subcutaneous myiasis in several animal species. The objective of the present investigation was to identify and characterize morphologically and molecularly the larvae of Hypoderma spp. collected from cattle (Bos taurus taurus) and red deer (Cervus elaphus) in the district of Castelo Branco, Portugal. For this purpose, a total of 8 larvae were collected from cattle (n=2) and red deer (n=6). After morphological identification of Hypoderma spp. larvae, molecular characterization was based on PCR-RFLP and mitochondrial CO1 gene sequence analysis. All larvae were morphologically characterized as the third instar larvae (L3) of H. actaeon. Two restriction enzymes were used for molecular identification of the larvae. TaqI restriction enzyme was not able to cut H. actaeon. However, MboII restriction enzyme differentiated Hypoderma species showing 210 and 450 bp bands in H. actaeon. Furthermore, according to the alignment of the mt-CO1 gene sequences of Hypoderma species and to PCR-RFLP findings, all the identified Hypoderma larvae were confirmed as H. actaeon. This is the first report of identification of Hypoderma spp. (Diptera; Oestridae) from cattle and red deer in Portugal, based on morphological and molecular analyses.

Radiopacity of restorative composites by conventional radiograph and digital images with different resolutions

Raquel Venâncio Fernandes Dantas,Hugo Ramalho Sarmento,Rosângela Marques Duarte,Sônia Saeger Meireles Monte Raso,Ana Karina Maciel de Andrade,Maria Luiza dos Anjos Pontual 대한영상치의학회 2013 Imaging Science in Dentistry Vol.43 No.3

Purpose: This study was performed to evaluate and compare the radiopacity of dentin, enamel, and 8 restorative composites on conventional radiograph and digital images with different resolutions. Materials and Methods: Specimens were fabricated from 8 materials and human molars were longitudinally sectioned 1.0 mm thick to include both enamel and dentin. The specimens and tooth sections were imaged by conventional radiograph using #4 sized intraoral film and digital images were taken in high speed and high resolution modes using a phosphor storage plate. Densitometric evaluation of the enamel, dentin, restorative materials, a lead sheet, and an aluminum step wedge was performed on the radiographic images. For the evaluation, the Al equivalent (mm) for each material was calculated. The data were analyzed using one-way ANOVA and Tukey’s test (p⁄0.05), considering the material factor and then the radiographic method factor, individually. Results: The high speed mode allowed the highest radiopacity, while the high resolution mode generated the lowest values. Furthermore, the high resolution mode was the most efficient method for radiographic differentiation between restorative composites and dentin. The conventional radiograph was the most effective in enabling differentiation between enamel and composites. The high speed mode was the least effective in enabling radiographic differentiation between the dental tissues and restorative composites. Conclusion: The high speed mode of digital imaging was not effective for differentiation between enamel and composites. This made it less effective than the high resolution mode and conventional radiographs. All of the composites evaluated showed radiopacity values that fit the ISO 4049 recommendations.

Radiopacity of restorative composites by conventional radiograph and digital images with different resolutions

Dantas, Raquel Venancio Fernandes,Sarmento, Hugo Ramalho,Duarte, Rosangela Marques,Meireles Monte Raso, Sonia Saeger,de Andrade, Ana Karina Maciel,Dos Anjos-Pontual, Maria Luiza Korean Academy of Oral and Maxillofacial Radiology 2013 Imaging Science in Dentistry Vol.43 No.3

Purpose: This study was performed to evaluate and compare the radiopacity of dentin, enamel, and 8 restorative composites on conventional radiograph and digital images with different resolutions. Materials and Methods: Specimens were fabricated from 8 materials and human molars were longitudinally sectioned 1.0 mm thick to include both enamel and dentin. The specimens and tooth sections were imaged by conventional radiograph using #4 sized intraoral film and digital images were taken in high speed and high resolution modes using a phosphor storage plate. Densitometric evaluation of the enamel, dentin, restorative materials, a lead sheet, and an aluminum step wedge was performed on the radiographic images. For the evaluation, the Al equivalent (mm) for each material was calculated. The data were analyzed using one-way ANOVA and Tukey's test (p<0.05), considering the material factor and then the radiographic method factor, individually. Results: The high speed mode allowed the highest radiopacity, while the high resolution mode generated the lowest values. Furthermore, the high resolution mode was the most efficient method for radiographic differentiation between restorative composites and dentin. The conventional radiograph was the most effective in enabling differentiation between enamel and composites. The high speed mode was the least effective in enabling radiographic differentiation between the dental tissues and restorative composites. Conclusion: The high speed mode of digital imaging was not effective for differentiation between enamel and composites. This made it less effective than the high resolution mode and conventional radiographs. All of the composites evaluated showed radiopacity values that fit the ISO 4049 recommendations.

Healthcare Workers' Mental Health in Pandemic Times: The Predict Role of Psychosocial Risks

Barros Carla,Baylina Pilar,Fernandes Rúben,Ramalho Susana,Arezes Pedro 한국산업안전보건공단 산업안전보건연구원 2022 Safety and health at work Vol.13 No.4

Background: Healthcare workers perform an emotionally exhausting daily work activity, making them prone to occupational hazards, namely psychosocial ones. This study aims to assess the impact of psychosocial risk factors on healthcare workers’ mental health. Methods: A cross-sectional study was developed between May and June of 2021 with 479 healthcare workers from Portuguese hospitals. The Depression, Anxiety and Stress Scale was used to assess mental health, and psychosocial risks were assessed through the Health and Work Survey e INSAT. Statistical analysis was performed to identify the psychosocial risk factors related to anxiety, depression, and stress. Subsequently, a multiple linear regression was performed to identify the models that better explained psychosocial risk factors’ relationship with anxiety, depression, and stress. Results: Data showed a strong exposure to psychosocial risks. Work pace and intensity, work relationships, and emotional demands stood out with higher global average percentages for yes answers to “exposure and discomfort.” The analysis of the b values and p-values from the multiple linear regression shows that some cross-sectional psychosocial risks are predictors of anxiety and stress dimensions, and other psychosocial risks differ in the two mental health dimensions. However, it is important to highlight that healthcare workers still showed great joy and pleasure in performing their work activities. Conclusion: Support network development in the work environment is needed to prevent healthcare workers’ emotional stress and promote their psychological well-being. Therefore, new research is essential to understand the psychosocial risks that affect healthcare workers and assess the less visible effects of workehealth relationships.

Alveolar ridge augmentation with the perforated and nonperforated bone grafts

Erica Dorigatti de Avila,José Scarso Filho,Lizete Toledo de Oliveira Ramalho,Mario Francisco Real Gabrielli,Valfrido Antônio Pereira Filho 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.1

Purpose: Autogenous bones are frequently used because of their lack of antigenicity, butgood osteoconductive and osteoinductive properties. This study evaluated the biologicalbehavior of perforated and nonperforated cortical block bone grafts. Methods: Ten nonsmoking patients who required treatment due to severe resorption of thealveolar process and subsequent implant installation were included in the study. The inclusioncriteria was loss of one or more teeth; the presence of atrophy of the alveolar processwith the indication of reconstruction procedures to allow rehabilitation with dental implants;and the absence of systemic disease, local infection, or inflammation. The patientswere randomly divided into two groups based on whether they received a perforated (innersurface) or nonperforated graft. After a 6-month healing period, a biopsy was performedand osseointegrated implants were installed in the same procedure. Results: Fibrous connective tissue was evident at the interface in patients who receivednonperforated grafts. However, full union between the graft and host bed was visible inthose who had received a perforated graft. Conclusions: We found that cortical inner side perforations at donor sites increased thesurface area and opened the medullary cavity. Our results indicate an increased rate ofgraft incorporation in patients who received such perforated grafts.

Alveolar ridge augmentation with the perforated and nonperforated bone grafts

de Avila, Erica Dorigatti,Filho, Jose Scarso,de Oliveira Ramalho, Lizete Toledo,Real Gabrielli, Mario Francisco,Pereira Filho, Valfrido Antonio Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.1

Purpose: Autogenous bones are frequently used because of their lack of antigenicity, but good osteoconductive and osteoinductive properties. This study evaluated the biological behavior of perforated and nonperforated cortical block bone grafts. Methods: Ten nonsmoking patients who required treatment due to severe resorption of the alveolar process and subsequent implant installation were included in the study. The inclusion criteria was loss of one or more teeth; the presence of atrophy of the alveolar process with the indication of reconstruction procedures to allow rehabilitation with dental implants; and the absence of systemic disease, local infection, or inflammation. The patients were randomly divided into two groups based on whether they received a perforated (inner surface) or nonperforated graft. After a 6-month healing period, a biopsy was performed and osseointegrated implants were installed in the same procedure. Results: Fibrous connective tissue was evident at the interface in patients who received nonperforated grafts. However, full union between the graft and host bed was visible in those who had received a perforated graft. Conclusions: We found that cortical inner side perforations at donor sites increased the surface area and opened the medullary cavity. Our results indicate an increased rate of graft incorporation in patients who received such perforated grafts.

Thioanisole oxidation promoted by new niobium-based catalyst: The effect of surface hydroxyl groups on catalytic performance

de Aguiar Guilherme Carletti,Batalha Daniel Carreira,Fajardo Humberto Vieira,Filho José Balena Gabriel,Bruziquesi Carlos Giovanni Oliveira,de Oliveira Luiz Carlos Alves,Gonçalves Mateus Aquino,Ramalho 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.10

In this work, the application of a new, synthesized niobium-based catalyst, called S4 (niobium oxyhydroxide), in the liquid-phase oxidation of methyl-phenyl sulfide (thioanisole) using hydrogen peroxide as oxidant was proposed. The synthetic method employed provided a material with low crystallinity and high specific surface area and acidity. A commercial material, called HY-340 (hydrated niobium oxide), was also employed as heterogeneous catalyst for comparative purposes. The results showed that the synthesized S4 material is an outstanding catalyst, being able to completely convert the substrate (thioanisole) that achieves almost 90% of selectivity for methyl phenyl sulfone formation, under mild reaction conditions. According to the theoretical and experimental combined results, the superior performance of S4 catalyst is related to the better interaction of H2O2 and thioanisole molecules with S4 surface, compared to HY-340, pointing to the greater ability of this catalyst to form reactive oxygen species in contact with hydrogen peroxide, due to its higher content of free hydroxyl groups present on its surface.