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Fibrinolytic and Antiplatelet Aggregation Properties of a Recombinant Cheonggukjang Kinase
Chinzorig Radnaabazar,박충무,김정환,차재호,송영선 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.6
This study characterized the efficacy of recombinant Cheonggukjang kinase (CGK) 3–5-rich fraction as a thrombolytic agent, which we overexpressed in Bacillus licheniformis ATCC10716, a strain normally lacking fibrinolytic activity. We found that CGK3-5 is a plasmin-like protease that directly degrades fibrin clots and does not activate plasminogen during fibrin clot lysis and platelet-rich clot lysis assays. We also confirmed antiplatelet and antithrombotic activity by CGK3-5-rich fraction both in vitro and in vivo. CGK3-5-rich fraction inhibited collagen-induced platelet aggregation in platelet-rich plasma in a concentration-dependent manner. The concentration of 1.5 mg/mL CGK3-5-rich fraction completely inhibited collagen-induced platelet aggregation. Furthermore, injection of CGK3-5-rich fraction into tail veins dose-dependently protected mice from death by pulmonary embolism induced by collagen and epinephrine. The survival rates were 30%, 70%, and 100%, respectively, with doses of 130 mg/kg, 260 mg/kg, and 520 mg/kg. These findings suggest that CGK3-5 holds promise as a treatment to mitigate the potentially effects of stroke and heart failure.
Hierarchical Cluster Analysis Histogram Thresholding with Local Minima
Sengee, Nyamlkhagva,Radnaabazar, Chinzorig,Batsuuri, Suvdaa,Tsedendamba, Khurel-Ochir,Telue, Berekjan Korea Multimedia Society 2017 The journal of multimedia information system Vol.4 No.4
In this study, we propose a method which is based on "Image segmentation by histogram thresholding using hierarchical cluster analysis"/HCA/ and "A nonparametric approach for histogram segmentation"/NHS/. HCA method uses that all histogram bins are one cluster then it reduces cluster numbers by using distance metric. Because this method has too many clusters, it is more computation. In order to eliminate disadvantages of "HCA" method, we used "NHS" method. NHS method finds all local minima of histogram. To reduce cluster number, we use NHS method which is fast. In our approach, we combine those two methods to eliminate disadvantages of Arifin method. The proposed method is not only less computational than "HCA" method because combined method has few clusters but also it uses local minima of histogram which is computed by "NHS".
Hye Yeon Cho,Chung Mu Park,Mi Jeong Kim,Radnaabazar Chinzorig,Chung Won Cho,Young Sun Song 한국영양학회 2011 Nutrition Research and Practice Vol.5 No.5
We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-α-stimulated human umbilical vascular endothelial cells (HUVECs). TNF-α exposure significantly increased expression of monocyte chemoattractant protein (MCP)-1, vascular adhesion molecule (VCAM)-1, and intercellular adhesion molecule-1. Genistein significantly decreased MCP-1 and VCAM-1 production in a dose-dependent manner, whereas CAM expression was not significantly lowered by genistein treatment. However, daidzein slightly decreased MCP-1 production. The effects of genistein and daidzein on MCP-1 secretion coincided with mRNA expression. Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-α exposure. A low concentration of isoflavones significantly inhibited nuclear factor (NF)κB activation, whereas a high dose slightly ameliorated these inhibitive effects. These results suggest that genistein had a stronger effect on MCP-1 and eNOS expression than that of daidzein. Additionally, NFκB transactivation might be partially related to the down-regulation of these mRNAs in TNF-α-stimulated HUVECs.
Heritability and linkage study on heart rates in a Mongolian population
Bayasgalan Gombojav,박한수,김종일,주영석,성주헌,조성일,이미경,Heechoul Ohrr,Janchiv Radnaabazar,서정선 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.5
Elevated heart rate has been proposed as an independent risk factor for cardiovascular diseases, but their interrelationships are not well understood. In this study, we performed a genome-wide linkage scan in 1,026 individuals (mean age 30.6 years, 54.5% women) from 73 extended families of Mongolia and determined quantitative trait loci that influence heart rate. The DNA samples were genotyped using deCODE 1,039 microsatellite markers for 3 cM density genome-wide linkage scan. Correlation analysis was carried out to evaluate the correlation of the covariates and the heart rate. T-tests of the heart rate were also performed on sex, smoking and alcohol intake. Consequently, this model was used in a nonparametric genome-wide linkage analysis using variance component model to create a multipoint logarithm of odds (LOD) score and a corresponding P value. In the adjusted model, the heritability of heart rate was estimated as 0.32 (P < .0001) and a maximum multipoint LOD score of 2.03 was observed in 77 cM region at chromosome 18. The second largest LOD score of 1.52 was seen on chromosome 5 at 216 cM. Genes located on the specified locations in chromosomes 5 and 18 may be involved in the regulation of heart rate.
Cho, Hye-Yeon,Park, Chung-Mu,Kim, Mi-Jeong,Chinzorig, Radnaabazar,Cho, Chung-Won,Song, Young-Sun The Korean Nutrition Society 2011 Nutrition Research and Practice Vol. No.
We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-${\alpha}$-stimulated human umbilical vascular endothelial cells (HUVECs). TNF-${\alpha}$ exposure significantly increased expression of monocyte chemoattractant protein (MCP)-l, vascular adhesion molecule (VCAM)-1, and intercellular adhesion molecule-1. Genistein significantly decreased MCP-l and VCAM-l production in a dose-dependent manner, whereas CAM expression was not significantly lowered by genistein treatment. However, daidzein slightly decreased MCP-l production. The effects of genistein and daidzein on MCP-l secretion coincided with mRNA expression. Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-${\alpha}$ exposure. A low concentration of isoflavones significantly inhibited nuclear factor (NF)${\kappa}$B activation, whereas a high dose slightly ameliorated these inhibitive effects. These results suggest that genistein had a stronger effect on MCP-l and eNOS expression than that of daidzein. Additionally, NF${\kappa}$B transactivation might be partially related to the down-regulation of these mRNAs in TNF-${\alpha}$-stimulated HUVECs.
Ryoo, Soo-Ryoon,Cho, Hyun-Jeong,Lee, Hye-Won,Jeong, Hey Kyeong,Radnaabazar, Chinzorig,Kim, Yeun-Soo,Kim, Min-Jeong,Son, Mi-Young,Seo, Hyemyung,Chung, Sul-Hee,Song, Woo-Joo Blackwell Publishing Ltd 2008 Journal of Neurochemistry Vol.104 No.5
<P>Abstract</P><P>Most individuals with Down Syndrome (DS) show an early-onset of Alzheimer’s disease (AD), which potentially results from the presence of an extra copy of a segment of chromosome 21. Located on chromosome 21 are the genes that encode &bgr;-amyloid (A&bgr;) precursor protein (<I>APP</I> ), a key protein involved in the pathogenesis of AD, and dual-specificity tyrosine(Y)-phosphorylation regulated kinase 1A (<I>DYRK1A</I> ), a proline-directed protein kinase that plays a critical role in neurodevelopment. Here, we describe a potential mechanism for the regulation of AD pathology in DS brains by DYRK1A-mediated phosphorylation of APP. We show that APP is phosphorylated at Thr668 by DYRK1A <I>in vitro</I> and in mammalian cells. The amounts of phospho-APP and A&bgr; are increased in the brains of transgenic mice that over-express the human DYRK1A protein. Furthermore, we show that the amounts of phospho-APP as well as those of APP and DYRK1A are elevated in human DS brains. Taken together, these results reveal a potential regulatory link between APP and DYRK1A in DS brains, and suggest that the over-expression of DYRK1A in DS may play a role in accelerating AD pathogenesis through phosphorylation of APP.</P>