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ZNF424, a novel human KRAB/C2H2 zinc finger protein, suppresses NFAT and p21 pathway
( Yue Qun Wang ),( Jun Mei Zhou ),( Xiang Li Ye ),( Yong Qi Wan ),( Yong Qing Li ),( Xiao Yan Mo ),( Wu Zhou Yuan ),( Yan Yan ),( Na Luo ),( Ze Qun Wang ),( Xiong Wei Fan ),( Yun Deng ),( Xiu Shan Wu 한국생화학분자생물학회 (구 한국생화학회) 2010 BMB Reports Vol.43 No.3
Zinc finger-containing transcription factors are the largest single family of transcriptional regulators in mammals, which play an essential role in cell differentiation, cell proliferation, apoptosis, and neoplastic transformation. Here we have cloned a novel KRAB-related zinc finger gene, ZNF424, encoding a protein of 555aa. ZNF424 gene consisted of 4 exons and 3 introns, and mapped to chromosome 19p13.3. ZNF424 gene was ubiquitously expressed in human embryo tissues by Northern blot analysis. ZNF424 is conserved across species in evolution. Using a GFP-labeled ZNF424 protein, we demonstrate that ZNF424 localizes mostly in the nucleus. Transcriptional activity assays shows ZNF424 suppresses transcriptional activity of L8G5-luciferase. Overexpression of ZNF424 in HEK- 293 cells inhibited the transcriptional activity of NFAT and p21, which may be silenced by siRNA. The results suggest that ZNF424 protein may act as a transcriptional repressor that suppresses NFAT and p21 pathway to mediate cellular functions. [BMB reports 2010; 43(3): 212-218]
MiR-675 Promotes the Growth of Hepatocellular Carcinoma Cells Through Cdc25A Pathway
Yu, Ya-Qun,Weng, Jun,Li, Shu-Qun,Li, Bo,Lv, Jun Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8
Background: MicroRNAs (miRNAs) have fundamental roles in tumorigenesis. MiR-675 is upregulated in hepatocellular carcinoma(HCC) cells. However, the roles of miR-675 in hepatocellular carcinogenesis are still not fully elucidated. In this study, we focus on investigating the effect and mechanism of miR-675 in proliferation of HCC cells. Materials and Methods: The cell proliferation was measured by MTT assays after transfection with miR-675 inhibitor and miR-675 mimics in HCC cells. The expression level of miR-675 was detected by real-time quantitative reverse transcription polymerase chain reaction. Protein expression of Cdc25A was measured by western blotting analysis. Results: In MTT assays, overexpression of miR-675 promoted the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675mimics control group. Downexpression of miR-675 inhibited the proliferation of HCC cells(P<0.05. at 48 hours, P<0.01. at 72 hours) compared with the miR-675inhibitor control group. In western blotting analysis, the expression level of Cdc25A was significantly increased (p<0.05) after treatment with miR-675 mimics. The expression level of Cdc25A was significantly decreased (p<0.05) after treatment with miR-675 inhibitor. Conclusions: Our results indicate that miR-675 promotes the proliferation in human hepatocellular carcinoma cells by associating with Cdc25A signaling pathway.
Maximum Likelihood and Signal-Selective TDOA Estimation for Noncircular Signals
Fei Wen and Qun Wan,Qun Wan 한국통신학회 2013 Journal of communications and networks Vol.15 No.3
This paper addresses the issue of time-difference-ofarrival (TDOA) estimation for complex noncircular signals. First,under the wide-sense stationary assumption, we derive the maximum likelihood (ML) estimator and the Cramer-Rao lower bound for Gaussian noncircular signals in Gaussian circular noise. The ML estimator uses the second-order statistics information of a noncircular signal more comprehensively when compared with the cross-correlation (CC) and the conjugate CC estimators. Further,we present a scheme to modify the traditional signal-selective TDOA methods for noncircular signals on the basis of the cyclostationarity of man-made signals. This scheme simultaneously exploits the information contained in both the cyclic cross-correlation (CCC) and the conjugate CCC of a noncircular signal.
Modelling the Construction of a High Embankment Dam
Qun Chen,Yu-Hua Zou,Min Tang,Chang-Rong He 대한토목학회 2014 KSCE Journal of Civil Engineering Vol.18 No.1
Appropriate layer quantity has been considered to model the sequential construction of a high embankment dam over 300 m and its effect on the predicted settlement of the 314 m high Shuangjiangkou dam has been discussed. The simulation results have demonstrated that at least 25 layers are required to accurately model the stage construction of a high embankment dam over 300 m. The stress and deformation within the dam and the foundation during the dam construction and reservoir filling have been simulated using finite element analysis. Saturated-unsaturated seepage theory is used to analyse the transient seepage field in the dam and in the foundation. Two different cases about the construction and the operation are modelled. One involves gradual reservoir filling after the completion of sequential construction, whereas the other involves sequential construction and reservoir impounding by several interleaved stages. The simulation results for both cases have been compared and discussed.
Qun-Ying Jin,Hua-Zheng Peng,Er-Pei Lin,Nan Li,Dan-Ni Huang,Yan-Li Xu,Xi-Qi Hua,Kui-Hong Wang,Tang-Jun Zhu 한국식물학회 2016 Journal of Plant Biology Vol.59 No.4
As one of the largest members of Poaceae family, bamboo is a very important agricultural plant in the world. The development of bamboo shoot is very special and particularly significant to bamboo production. Understanding the developmental differences between bamboo shoot and rhizome shoot is extremely valuable for us to further elucidate the mechanism of bamboo shoot formation since both bamboo shoot and rhizome shoot develop directly from rhizome bud underground. In this paper, miRNA chips with 413 miRNA probes were used to compare miRNA expressions between bamboo shoot and rhizome shoot. The experiment revealed 64 bamboo shoot upregulated and 56 rhizome shoot up-regulated miRNAs which were classified into four major categories according to deep sequencing based target prediction. Meristem and morphological development related miRNAs were most important in bamboo shoot, especially miR171 and miR156 members. While in rhizome shoot the mainstream of miRNA expressions was metabolism and nutrition related ones, especially miR395 members. The meristem and morphological development related miRNAs in bamboo shoot showed some embryonic characteristics and suggested the participation of several phytohormones like gibberellin, cytokinin and auxin, which were absent in those miRNAs of rhizome shoot. Further qRT-PCR detections of 21 up-regulated miRNAs in bamboo seedlings indicated that 12 ones were regulated to varying degrees by some environmental factors. Among them, rhizome shoot upregulated osa-miR395b was the most environment-sensitive miRNA, particularly to dehydration. And the bamboo shoot up-regulated osa-miR399j proved uniquely and strongly induced by phosphor. The existence of multiple regulation sites from same miRNA suggested the probability of crosstalks among meristem development, metabolism and stress response during bamboo shoot and rhizome shoot development.
Thermal Shock Behavior of a Novel TiCx–Ni3(Al,Ti)/Ni Functionally Graded Composite
Qun Yu,Zhenying Huang,Guangming Zheng,Wenqiang Hu,Cong Lei,Yuanbo Wang,Yidan Jiao,Hongxiang Zhai 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12
This paper focuses on the study of thermal shock behaviors of a novel TiCx–Ni3(Al,Ti)/Ni functionally graded material (FGM)fabricated from Ti3AlC2and Ni powders. Thermal shock resistance of resulted FGM was conducted by water quenching atdifferent temperatures, mechanical properties and microstructures after thermal shocks were correspondingly determined andcharacterized. The microstructure of the FGM after thermal shocks shows no cracks of instability failure between the transitionzones, and the reinforcement phases were closely combined with the matrix. Meanwhile, compared with the strengthof 1329 MPa without thermal shock, when the temperature of the thermal shock reached 800 °C and 1000 °C respectively,with 10 repeated heat-water quench cycles, the residual flexural strength of FGM decreased slightly, but it could still reach1100 MPa. Notably, the strength can still maintain at 843 MPa after 10 cycles of thermal shock at 1200 °C. Such excellentthermal shock performance may be the result of the good interfacial bonding and the special gradient structure, the oxidationlayer also plays a protective role.