Combination of Tumor Volume and Epstein-Barr Virus DNA Improved Prognostic Stratification of Stage II Nasopharyngeal Carcinoma in the Intensity Modulated Radiotherapy Era: A Large-Scale Cohort Study

Qiu-Yan Chen,Shao-Yan Guo,Lin-Quan Tang,Tong-Yu Lu,Bo-Lin Chen,Qi-Yu Zhong,Meng-Sha Zou,Qing-Nan Tang,Wen-Hui Chen,Shan-Shan Guo,Li-Ting Liu,Yang Li,Ling Guo,Hao-Yuan Mo,Rui Sun,Dong-Hua Luo,Chong Zha 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3

Purpose Little is known about combination of the circulating Epstein-Barr viral (EBV) DNA and tumor volume in prognosis of stage II nasopharyngeal carcinoma (NPC) patients in the intensity modulated radiotherapy (IMRT) era. We conducted this cohort study to evaluate the prognostic values of combining these two factors. Materials and Methods By Kaplan-Meier, we compare the differences of survival curves between 385 patients with different EBV DNA or tumor volume levels, or with the combination of two biomarkers mentioned above. Results Gross tumor volume of cervical lymph nodes (GTVnd, p < 0.001) and total tumor volume (GTVtotal, p < 0.001) were both closely related to pretreatment EBV DNA, while gross tumor volume of nasopharynx (GTVnx, p=0.047) was weakly related to EBV DNA. EBV DNA was significantly correlated with progress-free survival (PFS, p=0.005), locoregional-free survival (LRFS, p=0.039), and distant metastasis-free survival (DMFS, p=0.017), while GTVtotal, regardless of GTVnx and GTVnd, had a significant correlation with PFS and LRFS. The p-values of GTVtotal for PFS and LRFS were 0.008 and 0.001, respectively. According to GTVtotal and pretreatment EBV DNA level, patients were divided into a low-risk group (EBV DNA 0 copy/mL, GTVtotal < 30 cm3; EBV DNA 0 copy/mL, GTVtotal  30 cm3; or EBV DNA > 0 copy/mL, GTVtotal < 30 cm3) and a high-risk group (EBV DNA > 0 copy/mL, GTVtotal  30 cm3). When patients in the low-risk group were compared with those in the high-risk group, 3-year PFS (p=0.003), LRFS (p=0.010), and DMFS (p=0.031) rates were statistically significant. Conclusion Pretreatment plasma EBV DNA and tumor volume were both closely correlated with prognosis of stage II NPC patients in the IMRT era. Combination of EBV DNA and tumor volume can refine prognosis and indicate for clinical therapy.

Metatranscriptomic sequencing reveals there were abundant and diverse viruses in the midgut of diseased silkworm (Bombyx mori) with soft rot symptoms

Qiu Qunnan,Pan Jun,Kumar Dhiraj,Wei Shulin,Tong Xinyu,Zhu Min,Hu Xiaolong,Gong Chengliang 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.4

To date, six viruses belonging to genera Alphabaculovirus, Cypovirus, Iflavirus, Iteravirus, Bidensovirus and Maculavirus were harbored in the silkworm (Bombyx mori) and in the cultured BmN cells. Diseased silkworms with unknown etiology often create economic losses to the farmers. In the present investigation, the abundance and diversity of viruses in the midgut of diseased silkworms with soft rot symptoms were assessed by meta transcriptomic sequencing in order to screen the potential pathogenic virus of the silkworm, B. mori. After removal of undefined reads, clean reads were assembled, and the obtained 76 viral unigenes were annotated to 43 viruses. Among these viruses, the B. mori cypovirus, Pieris rapae virus, Nodaviridae sp., B. mori bidensovirus, Pseudomonas virus pf16, Pseudomonas phage Skulduggery, Klebsiella phage KPN U2874, Equid gamma herpesvirus 2, fowlpox virus and Xestia c-nigrum granulovirus were dominant virus species. These metatran scriptomic sequencing results provided new clues for screening and identifying novel pathogenic virus of the silkworm.

The Influence of Hi-flux Powders Characteristics on the Performance of Magnetic Powder Cores

Zhao Tong Chun,Ma Hong Qiu,Ding Fu Chang 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1

The influence of Hi-flux powders characteristics on the performance of magnetic powder cores was studied. It was found that different cooling rate and nozzle configuration could change the shape and microstructure of powders. Smooth surface and spherical shape of powders were beneficial to improve DC bias performance and reduce core losses of magnetic powder core.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Lei Wang,Qiu-Tong Wang,Yu-Peng Liu,Qing-Qing Dong,Hai-Jie Hu,Zhi Miao,Shuang Li,Yong Liu,Hao Zhou,Tong-Cun Zhang,Wen-Jian Ma,Xuegang Luo 대한위암학회 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Radon-induced demethylation of Cdk2 CpG island in the rat lung

Cheng Wu,Qiu Chen,Jian Tong,Xin Xie,Fengmei Cui,Yang Jiao,Dandan Qi,Jihua Nie,Tom K. Hei,Qisheng Jiang 한국유전학회 2014 Genes & Genomics Vol.36 No.6

Radon exposure has been linked to lung carcinogenesisin both human and animal studies. The identificationof sequential changes in DNA methylation duringtumour progression and the elucidation of their interplaywith genetic changes will broaden our molecular understandingof this disease. Rats were exposed to 120 or 400working level months (WLM) of radon, lung pathologicalchanges were examined by haematoxylin and eosin staining,lung single cell suspension cell cycles were detected byflow cytometry, lung cell cycle regulated gene (Cdkn2a,P53, Cdk4/2, Mdm4/2 and Rb1 genes) expression wasquantified by real-time PCR and methylation of CpG islandsin the promoters of cell cycle-regulated genes were detectedby bisulfite sequencing PCR. The alveolar walls of rat lungsafter exposured to radon exhibited papillae and the lungbronchial epithelial cells stained positively for proliferatingcell nuclear antigen. The bronchial epithelial cells displayedsome hyperplasia after challenged by 400 WLM of radon. Moreover, G1 arrest decreased; Rb1, Mdm2/4, and Cdk2/4expression decreased and Cdk2 was demethylated at thesecond and sixth CpG loci from base pairs 3092704 to3092953 of chromosome 7. Cdk2 demethylation may beapplicable as a biomarker of early lung damage that wasinduced by radon and other environmental carcinogens.

Cooperative Optimization of Bus-Subway-Shared Bicycle Based on Uncertainty Theory

Yunqiang Xue,Tong He,Xiangming Yao,Bing Zhang,Hongzhi Guan,Yang Qiu 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4

This research considered the uncertainty of the operation time of shared bicycle, constructed the urban coupling network, and established the optimization model for the cooperative operation of conventional buses, subways and shared bikes. In the programming model, the optimization objective is to minimize the total travel time of passengers, and by optimizing and adjusting the departure intervals of conventional buses and subways as well as the stopping time of vehicles, the travel efficiency of passengers is improved. The interchange cooperative system composed of Nanchang Metro Line 1 and Bus 520 and the surrounding shared bicycle concentration area was taken as the case study. According to the optimization results, the passenger waiting time, passenger transfer time, and total passenger on-board time are effectively reduced by 21.5%, 23.2%, and 7.37%; ultimately, the total travel time is reduced by 8.2% at a confidence level of 95%. Case analyses show that the conventional bus stopping time has the greatest impact on the total travel time of the passengers as compared to the other variables. The research is of positive practical significance for promoting the efficient and coordinated development of urban transportation networks and facilitating the travel of residents.

Field and Laboratory Investigation of Pullout Resistance of Steel Anchors in Rock

Park, Jiho,Qiu, Tong,Kim, Younguk American Society of Civil Engineers 2013 Journal of geotechnical and geoenvironmental engin Vol.139 No.12

Efficiency Optimization of Variable Iron Loss Resistance Asynchronous Motor Based on Grey Wolf Optimization Algorithm

Guo Zhen,Tong Di,Zhao Yan-cheng,Chen Shuang,Nai Ji-qiu,Ye Ming-hao 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

Asynchronous motor is widely used in various industrial fields. When asynchronous motor works in case close to the rated load, it will run at maximum efficiency. However, most of the load is 50–100% of rated load and it will lead to the serious waste of energy. This paper proposes an efficiency optimization control strategy based on the grey wolf optimization algorithm to improve the operating efficiency of asynchronous motors at light loads. The motor loss model considering the change of iron loss is established and it makes the asynchronous motor model more accurate. The grey wolf optimization algorithm is used to find the optimized flux value. When the asynchronous motor operates at the optimal flux, the loss power of the motor is decreased and the operation efficiency improves effectively. The proposed method reduces the flux search time and improves the stability of the system. The simulation model is established and the simulation results are provided to verify the feasibility of the proposed control strategy achieving the global efficiency optimum of asynchronous motor.

An Analysis of the Focus Variation Microscope and Its Application in the Measurement of Tool Parameter

Lin Yuan,Tong Guo,Zhongjun Qiu,Xing Fu,Xiaotang Hu 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.12

Ultra-precision manufacturing is essential to the production of workpieces of the highest quality in terms of form accuracy, surface accuracy, and integrity. Compared with physical and chemical material removal methods, it has the advantages of high efficiency, high flexibility, and low cost. Because of the direct interaction between the workpiece and the tool during the process of ultra-precision manufacturing, the geometrical parameters of the tool directly affect the surface quality of the workpiece and need to be measured accurately and comprehensively. However, due to the large surface slopes of some tools, some three-dimensional measurement methods cannot measure their geometrical parameters because of limited maximum measurement angle. In this work, we build a focus variation system to make three-dimensional measurements of tools and extract their geometrical parameters. The data processing procedures and the selection of experimental parameters are analyzed in detail using simulations and experiments. Furthermore, we use a step height standard to verify the high accuracy of the measurement system and a roughened fl at surface to evaluate the measurement noise and vertical resolution. Measurements of the parameters of a round nose cutting tool and the surface texture parameters of a grinding tool are conducted and indicate that the focus variation system is suitable for measuring the geometrical parameters of tools for ultra-precision manufacturing.

Yunnan Red Pear-Yunhongli No.1 (YH-1)

Qun Shu,Minghua Qiu,Penglin Wang,Menglin Tong 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-