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- 저자 : Qionglin (검색결과 3 건)
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Weixing Wang,Lili Li,Mingyu Ding,Guoan Luo,Qionglin Liang 한국바이오칩학회 2018 BioChip Journal Vol.12 No.2
The oxygen tensions and matrix stiffness play important roles in regulating cell response to cytotoxic drugs. In recent years, single spatiotemporal oxygen or matrix stiffness gradient has been established by various technologies for cell studies. However, the synergistic effects of the two factors on tumor cells remained elusive. In this study, we created a highly integrated and easy-to-operate microfluidic device. It was capable of generating more continuous, linear, stable and diffusive hydrogel stiffness gradient over a well-defined oxygen gradient. Sodium hydroxide and pyrogallol were used to scavenge oxygen and generate oxygen gradient, skillfully avoiding the utilization of bulky pressurized gas cylinders and sophisticated flow control by conventional methods. Utilizing the newly developed microfluidic device, we successfully performed drug test with a hypoxia sensitive anti-cancer drug, triapazamine (TPZ), on A549 cells under perpendicular construction of oxygen and spatially hydrogel stiffness gradients. The cell experiment results demonstrated the matrix stiffness-dependent cell drug resistance and hypoxia-induced cytotoxicity of TPZ. In summary, the developed microfluidic device exhibits high potential in the study of matrix stiffness-dependent cell responses and oxygen-sensitive drug cytotoxicity. Furthermore, the design principle can be functionally extended to other dual factors system and will facilitate the study of cell response in complex physiological and pathological conditions.
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Dawei Ying,Kai Zhang,Naipeng Li,Xiaoni Ai,Qionglin Liang,Yiming Wang,Guoan Luo 한국바이오칩학회 2012 BioChip Journal Vol.6 No.3
A droplet-based microfluidic device for long-term culture and longitudinal observation of Caenorhabditis eleganswas presented. The worms were encapsulated in W/O droplets and controllably introduced into separate chambers, where the medium/oil or medium/air interface can be manipulated by a gravity -actuated technique, for lifelong culture. This device also contained clamp structures for reversibly immobilization of each worm separately, and thus we were able to track more details about each worm. Several phenotypes of worms were monitored from their fourth larva stage to death. The relationship between worm body length, stroke frequency and lifespan with this device was then studied. This device had a potential in various longitudinal researches on C. elegans including aging, drug screening, toxicity evaluation and etc.
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Huiyuan Guan,Jundong Wang,Siyuan Tan,Qiang Han,Qionglin Liang,Mingyu Ding 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.6
A novel and uncomplicated synthesis method of Cu2+-chelating with carboxyl groups that directly-modified NiFe2O4 magnetic microspheres (NiFe2O4-PAA-Cu2+) was fabricated for selective enrichment and separation of bovine hemoglobin (BHb). First, a carboxyl group directly-modified on NiFe2O4 magnetic microspheres was gained through a facile one-pot solvothermal method. Second, Cu2+ from CuSO4 was brought into use to react with carboxyl groups under mechanical stirring at room temperature. The resulting magnetic microspheres were characterized by distinct instruments that included transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM) and scanning electron microscope (SEM) to examine the size, morphology, composition and magnetization characterization. The results indicated that the NiFe2O4-PAA-Cu2+ microspheres exhibited good saturation magnetization (36.686 emu g1), which can facilitate magnetic separation under the help of an outside magnetic field. Also, good dispersion and high adsorption ability to BHb (783.53mg g1) can be applied to selective enrichment for bovine hemoglobin and used for selective sorption of BHb protein in bovine blood samples.
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