Comparison of Hybridization Behavior between Double and Single Strand of Targets and the Application of Asymmetric PCR Targets in cDNA Microarray

( Qing Wei ),( San Zhen Liu ),( Jian Feng Huang ),( Xue Ying Mao ),( Xiao Hui Chu ),( Yu Wang ),( Min Yan Qiu ),( Yu Min Mao ),( Yi Xie ),( Yao Li ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.4

Double stranded targets on the cDNA microarray contain representatives of both the coding and noncoding strands, which will introduce hybridization competition with probes. Here, the effect of double and single strands of targets on the signal intensity and the ratios of Cy5/Cy3 within the same slide were compared. The results show that single stranded targets can increase the hybridization efficiency without changing the Cy5/Cy3 ratio. Based on these results, a new strategy was established by generating cDNA targets with asymmetric PCR, instead of conventional PCR, to increase the sensitivity of the cDNA microarray. Furthermore, the feasibility of this approach was validated. The results indicate that the cDNA microarray system based on asymmetric PCR is more sensitive, with no decrease in the reliability and reproducibility as compared with that based on conventional symmetric PCR.

Increased accumulation of anthocyanins in transgenic potato tubers by overexpressing the 3GT gene

Qing Wei,Qing Yang,Quan-Yi Wang,Zhi-Hang Feng,Bing Wang,Yun-Feng Zhang 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.1

In order to explore a biotechnological method for improving potato tuber color and creating plants with increased anthocyanin contents, a potato UDP-glucose: flavonoid-3-O-glucosyltransferase (3GT) gene was inserted behind the GBSSI promoter of pBin19, and this construct was introduced into Solanum tuberosum L. cultivar De´sire´e plants by Agrobacterium-mediated transformation. Six independent transgenic lines overexpressing the 3GT gene were identified by PCR and Southern blot analysis from 18 kanamycin-resistant plants. Due to the expression of 3GT gene, the tuber color and the anthocyanin content were enhanced noticeably in the transgenic plants compared to the wild-type control plants. This result suggests that the 3GT gene can potentially be used to improve potato color and enhance levels of antioxidants in the diet.

A Spectroscopic Study on Singlet Oxygen Production from Different Reaction Paths Using Solid Inorganic Peroxides as Starting Materials

Qingwei Li*,Fang Chen,Weili Zhao,Mingxiu Xu,Benjie Fang,Yuelong Zhang,Liping Duo,Yuqi Jin,Fengting Sang 대한화학회 2007 Bulletin of the Korean Chemical Society Vol.28 No.10

Using solid inorganic peroxides (including Li2O2, Na2O2, SrO2 and BaO2) as starting materials, three reaction paths for singlet oxygen (1O2) production were developed and studied. Their 1O2 emission spectra in the near-IR region and visible region from these reaction paths were simultaneously recorded by a near-IR sensitive Optical Multichannel Analyzer and a visible sensitive Optical Spectrum Analyzer, respectively. The comparison of their 1O2 emission spectra indicated that: (1) in term of the efficiency for 1O2 production, the gas-liquid-solid reaction path (in which Cl2 or HCl and H2O reacted with the solid inorganic peroxides suspension in CCl4) was prior to the gas-solid reaction path (in which Cl2 or HCl reacted with the solid inorganic peroxides suspension in CCl4), but was inferior to the gas-liquid reaction path (in which Cl2 or HCl reacted with the solid inorganic peroxides solution in H2O or D2O); (2) the alkali metal peroxides (such as Li2O2 and Na2O2) was prior to the alkaline earth metal peroxides (such as SrO2 and BaO2) as the solid reactants, and Cl2 was favorable than HCl as the gas reactant in efficiency for 1O2 production in these reaction paths.

Does Environmental Responsibility Lower ‘Double Hurdle’? Emerging Multinationals in Global Natural-Resource Industry

Qingwei NAN,Bo Kyung KIM,Jooyoung KWAK 한국유통과학회 2023 Asian Journal of Business Environment (AJBE) Vol.13 No.4

Purpose: Emerging markets under industrialization have become increasingly influential over the global natural-resource transactions. However, their average deal completion rates have been relatively low. The international business (IB) literature regards the low rate as evidence of ‘double hurdle’, the extra disadvantages in doing overseas business for firms from developing countries. Because legitimacy building mitigates liability of foreignness, we argue that an acquirer’s environmental responsibility effectively builds legitimacy. Research design, data and methodology: Stakeholders in the host country spread the acquirer’s environmental responsibility so that, by raising legitimacy, they may strengthen the link between environmental responsibility and deal completion. Our dataset consists of the 608 cross-border acquisition deals announced by the 196 firms in Brazil, Russia, India, and China over 2008-2019 period. Results: A logit regression result confirms that environmental responsibility increases the likelihood of acquisition deal completion. Also, hostmarket stakeholders positively moderate the relationship between environmental responsibility and the likelihood of deal completion. Conclusions: Overall, this study contributes to the IB literature by identifying environmental responsibility as a key approach to lowering the double hurdle in internationalization of firms in emerging markets. Any emerging multinationals interested in the foreign, brownfield entries to the natural-resource industries must enhance the environmental responsibility, which turns out extremely important.

Disturbance Attenuation Using Adaptive Disturbance Observer in HDDs

QingWei lia,Fei Wang 한국자기학회 2008 한국자기학회 학술연구발표회 논문개요집 Vol.- No.-

Investigation of Low-Frequency Characteristics of Four-Switch Three-Phase Inverter

Qingwei Yuan,Chong Cheng,Rongxiang Zhao 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.4

The low-frequency characteristics of four-switch three-phase (FSTP) inverter are investigated in this paper. Firstly, a general space vector pulse width modulation (SVPWM) directly involved the neutral point voltage of DC-link is proposed, where no sector identifications and trigonometric function calculations are needed. Subsequently, to suppress the DC offset in the neutral point voltage, the relationship between the neutral point voltage and the β-axis component of the load current is derived, and then a new neutral point voltage control scheme is proposed where no low pass filter is adopted. Finally, the relationship between the load power factor and the maximum linear modulation index of the FSTP inverter is revealed. Since the operational region for the FSTP inverter in low frequency is reduced by the enlarged amplitude of the neutral point voltage, a linear modulation range enlargement scheme is proposed. A permanent magnet synchronous motor with preset rotary speed serves as the low-frequency load of the FSTP inverter. Experimental results verify that the new neutral point voltage control scheme is effective in the deviation suppression of the neutral point voltage, and the proposed scheme is able to provide a larger linear operational region in low frequency.

Direct Stator Flux Vector Control Strategy for IPMSM using a Fullorder State Observer

Qingwei Yuan,Zhiyong Zeng,Rongxiang Zhao 대한전기학회 2017 Journal of Electrical Engineering & Technology Vol.12 No.1

A direct stator flux vector control scheme in discrete-time domain is proposed in this paper for the interior permanent magnet synchronous motor (IPMSM) drive to remove the proportional-integral (PI) controller from the direct torque control (DTC) scheme applied to IPMSM and to obtain faster dynamic response and lower torque ripple output. The output of speed outer loop is used as the desired torque angle instead of the desired torque in the proposed scheme. The desired stator flux vector in dq coordinate is calculated with a given amplitude. The state-space equations in discrete-time for IPMSM are established, the actual stator flux vector is estimated in deadbeat manner by a full-order state observer, and then the closed-loop control is achieved by the pole placement. The stator flux error vector is utilized to calculate the reference stator voltage vector. Extracting the angle position and amplitude from the estimated stator flux vector and estimating the output torque are eliminated for the direct feedback control of the stator flux vector. The proposed scheme is comparatively investigated with a PI-SVM DTC scheme by experiment results. Experimental results show the feasibility and advantages of the proposed control scheme.

Does Environmental Responsibility Lower 'Double Hurdle'? Emerging Multinationals in Global Natural-Resource Industry

Qingwei NAN,Bo Kyung KIM,Jooyoung KWAK Korea Distribution Science Association 2023 Asian journal of business environment Vol.13 No.4

Purpose: Emerging markets under industrialization have become increasingly influential over the global natural-resource transactions. However, their average deal completion rates have been relatively low. The international business (IB) literature regards the low rate as evidence of 'double hurdle', the extra disadvantages in doing overseas business for firms from developing countries. Because legitimacy building mitigates liability of foreignness, we argue that an acquirer's environmental responsibility effectively builds legitimacy. Research design, data and methodology: Stakeholders in the host country spread the acquirer's environmental responsibility so that, by raising legitimacy, they may strengthen the link between environmental responsibility and deal completion. Our dataset consists of the 608 cross-border acquisition deals announced by the 196 firms in Brazil, Russia, India, and China over 2008-2019 period. Results: A logit regression result confirms that environmental responsibility increases the likelihood of acquisition deal completion. Also, host-market stakeholders positively moderate the relationship between environmental responsibility and the likelihood of deal completion. Conclusions: Overall, this study contributes to the IB literature by identifying environmental responsibility as a key approach to lowering the double hurdle in internationalization of firms in emerging markets. Any emerging multinationals interested in the foreign, brownfield entries to the natural-resource industries must enhance the environmental responsibility, which turns out extremely important.

Phosphate Solubilization and Gene Expression of Phosphate-Solubilizing Bacterium Burkholderia multivorans WS-FJ9 under Different Levels of Soluble Phosphate

( Qingwei Zeng ),( Xiaoqin Wu ),( Jiangchuan Wang ),( Xiaolei Ding ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.4

Phosphate-solubilizing bacteria (PSB) have the ability to dissolve insoluble phosphate and enhance soil fertility. However, the growth and mineral phosphate solubilization of PSB could be affected by exogenous soluble phosphate and the mechanism has not been fully understood. In the present study, the growth and mineral phosphate-solubilizing characteristics of PSB strain Burkholderia multivorans WS-FJ9 were investigated at six levels of exogenous soluble phosphate (0, 0.5, 1, 5, 10, and 20 mM). The WS-FJ9 strain showed better growth at high levels of soluble phosphate. The phosphate-solubilizing activity of WS-FJ9 was reduced as the soluble phosphate concentration increased, as well as the production of pyruvic acid. Transcriptome profiling of WS-FJ9 at three levels of exogenous soluble phosphate (0, 5, and 20 mM) identified 446 differentially expressed genes, among which 44 genes were continuously up-regulated when soluble phosphate concentration was increased and 81 genes were continuously down-regulated. Some genes related to cell growth were continuously up-regulated, which would account for the better growth of WS-FJ9 at high levels of soluble phosphate. Genes involved in glucose metabolism, including glycerate kinase, 2-oxoglutarate dehydrogenase, and sugar ABC-type transporter, were continuously down-regulated, which indicates that metabolic channeling of glucose towards the phosphorylative pathway was negatively regulated by soluble phosphate. These findings represent an important first step in understanding the molecular mechanisms of soluble phosphate effects on the growth and mineral phosphate solubilization of PSB.

Energy-efficient clustering algorithm based on game theory for wireless sensor networks

Liu, Qingwei,Liu, Mandan SAGE Publications 2017 INTERNATIONAL JOURNAL OF DISTRIBUTED SENSOR NETWOR Vol.13 No.11