Responsive manganese-based nanoplatform amplifying cGAS-STING activation for immunotherapy

Qingbin He,Runxiao Zheng,Junchi Ma,Luyang Zhao,Yafang Shi,Jianfeng Qiu 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background The activation of the cyclic guanosine monophosphate-adenosine monophosphate synthasestimulator of interferon genes (cGAS-STING) signaling pathway has attracted great attention for its ability to up-regulate innate immune response and thus enhance cancer immunotherapy. However, many STING agonists limit the further advancement of immunotherapy due to weak tumor responsiveness or low activation efficiency. The responsive and effective activation of cGAS-STING signaling in tumors is a highly challenging process. Methods In this study, a manganese-based nanoplatform (MPCZ NPs) was constructed that could responsively and efficiently generate more manganese ions (Mn2+) and reactive oxygen species (ROS) to activate cGAS-STING signaling pathway. Briefly, manganese dioxide (MnO2) was loaded with zinc protoporphyrin IX (ZPP) molecule and coated by polydopamine (PDA) embedded with NH4HCO3 to obtain MPCZ NPs. Additionally, MPCZ NPs were evaluated in vitro and in vivo for their antitumor effects by methyl thiazolyl tetrazolium (MTT) assay and TUNEL assays, respectively. Results In this system, tumor responsiveness was achieved by exogenous (laser irradiation) and endogenous (high levels GSH) stimulation, which triggered the collapse or degradation of PDA and MnO2. Moreover, the release of Mn2+ augmented the cGAS-STING signaling pathway and enhanced the conversion of hydrogen peroxide (H2O2) to hydroxyl radical (·OH) under NIR laser irradiation. Furthermore, the release of ZPP and the elimination of GSH by MPCZ NPs inhibited HO-1 activity and prevented ROS consumption, respectively. Conclusions This adopted open source and reduce expenditure strategy to effectively generate more ROS and Mn2+ to responsively activate cGAS-STING signaling pathway, providing a new strategy for improving immunotherapy.

Improvement of OPW-TR Algorithm for Compressing GPS Trajectory Data

( Qingbin Meng ),( Xiaoqiang Yu ),( Chunlong Yao ),( Xu Li ),( Peng Li ),( Xin Zhao ) 한국정보처리학회 2017 Journal of information processing systems Vol.13 No.3

Massive volumes of GPS trajectory data bring challenges to storage and processing. These issues can be addressed by compression algorithm which can reduce the size of the trajectory data. A key requirement for GPS trajectory compression algorithm is to reduce the size of the trajectory data while minimizing the loss of information. Synchronized Euclidean distance (SED) as an important error measure is adopted by most of the existing algorithms. In order to further reduce the SED error, an improved algorithm for open window time ratio (OPW-TR) called local optimum open window time ratio (LO-OPW-TR) is proposed. In order to make SED error smaller, the anchor points are selected by calculating point`s accumulated synchronized Euclidean distance (ASED). A variety of error metrics are used for the algorithm evaluation. The experimental results show that the errors of our algorithm are smaller than the existing algorithms in terms of SED and speed errors under the same compression ratio.

Improvement of OPW-TR Algorithm for Compressing GPS Trajectory Data

Meng, Qingbin,Yu, Xiaoqiang,Yao, Chunlong,Li, Xu,Li, Peng,Zhao, Xin Korea Information Processing Society 2017 Journal of information processing systems Vol.13 No.3

Massive volumes of GPS trajectory data bring challenges to storage and processing. These issues can be addressed by compression algorithm which can reduce the size of the trajectory data. A key requirement for GPS trajectory compression algorithm is to reduce the size of the trajectory data while minimizing the loss of information. Synchronized Euclidean distance (SED) as an important error measure is adopted by most of the existing algorithms. In order to further reduce the SED error, an improved algorithm for open window time ratio (OPW-TR) called local optimum open window time ratio (LO-OPW-TR) is proposed. In order to make SED error smaller, the anchor points are selected by calculating point's accumulated synchronized Euclidean distance (ASED). A variety of error metrics are used for the algorithm evaluation. The experimental results show that the errors of our algorithm are smaller than the existing algorithms in terms of SED and speed errors under the same compression ratio.

Valproic Acid Modulates the Multipotency in Periodontal Ligament Stem Cells via p53-Mediated Cell Cycle

엄소연,이호,Qingbin Zhang,김희영,이주희,서병무 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.2

Human periodontal ligament stem cells (PDLSCs), a type of mesenchymal stem cell, are a promising source for dental regeneration and are identified in human periodontal ligaments from extracted third molars. Valproic acid (VPA) is a histone deacetylase inhibitor that has been used as a wide-spectrum antiepileptic drug and a medication for mood disorders. VPA has shown several effects on increasing the pluripotency of embryonic stem cells and controlling osteogenic differentiation, besides the prevention of seizures. However, its effect on proliferation and osteogenesis depends on the cell type and concentration. The aim of this study was to investigate the effects of cyclic and constant VPA treatment on PDLSCs. Proliferation and apoptosis of PDLSCs were determined with cyclic and constant VPA treatment. In cemento/ osteogenic differentiation, osteogenic markers decreased significantly after cyclic treatment with 0.5 mM VPA. In contrast, VPA enhanced osteogenic differentiation after constant treatment. With cyclic VPA treatment, p53 levels related to apoptotic pathway decreased to induce proliferation. These findings indicated that VPA has different roles in proliferation and differentiation of PDLSCs in vitro and in vivo via p53-related pathway.

Analysis of Cable under Dynamic Contact and Large Deformation

Bingjian Wang,Qingbin Li,Tianyun Liu,Weibing Peng 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

For simulating contact interactions and high displacement gradients between the cable and the saddle at the middle tower of tripletower suspension bridges, a cable element is developed by combining the absolute nodal coordinate formulation and the quasiconforming technique. New curvature strains are developed and elastic forces are explicitly formulated for the cable elements. Thereafter, it is compared to the original one to verify its locking remedies. The numerical solutions using the element are compared to analytical results and solutions by the original element. Compared to the original, the proposed element suppresses the highfrequency disturbances in the velocity and acceleration curves. Using the element, the contact and sliding behavior between the cable and the saddle is analyzed by employing parameters obtained experimentally. The saddle’s mechanical and frictional performance subjected to different friction coefficients and unbalanced cable forces is investigated. The proposed model exhibits excellent accuracy in the prediction of the sliding force and the contact status between the cable and the saddle.

The Split-Permeation Grouting Mechanism of Loose and Broken Coal Rock Masses considering the Temporal and Spatial Characteristics of Slurry Viscosity

Hexuan Zhu,Lijun Han,Qingbin Meng,Jun Liu,Lingdong Meng,Wenlong Dong 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

Quick-setting grout has time-varying characteristics, and the space porosity of the injected medium changes after being grouted. The above two points need to be considered when loose and broken coal rock masses are grouted with rapid-setting grout. In other words, the coupling effect of the slurry-coal rock mass causes the slurry to exhibit the temporal and spatial characteristics of slurry viscosity (TSCSV). Based on the Bingham fluid constitutive model, a capillary group model with a linear increase in diameter was created, and a one-dimensional penetration grout diffusion model was established considering the TSCSV. The one-dimensional visualization splitting-permeation grout diffusion simulation test system was designed to obtain the space porosity of the broken coal rock mass under different final grouting pressures to obtain the law of the changes in grouting pressure and diffusion height with time under the condition of a fixed grouting rate. Furthermore, the mechanism of splitting-permeation grouting in loose and broken coal rock masses is analysed in depth considering the TSCSV. The results showed that the space porosity of coal rock masses increases linearly. In addition, the calculated value of the slurry diffusion distance was 0.93 − 1.1 times the experimental value considering the TSCSV. The calculated value was in good agreement with the experimental value. The calculated value of the final pressure of orifice grouting was approximately 1.5 times the test value without considering the unevenness of the space porosity distribution (USPD). The grouting pressure was overestimated without considering the USPD. Therefore, the TSCSV of the quick-setting slurry should be fully considered in the grouting design.

Molecular engineering of indoline based organic sensitizers for highly efficient dye-sensitized solar cells

Liu, Bo,Liu, Qingbin,You, Dan,Li, Xiaoyan,Naruta, Yoshinori,Zhu, Weihong The Royal Society of Chemistry 2012 Journal of materials chemistry Vol.22 No.26

<P>The increasing electron-donating ability of the donor part is focused to further optimize the light-harvesting capability. Our strategy is to introduce an additional donor group into the indoline unit in the donor part to form a donor–donor structure (D–D moiety). Three different units (carbazole, fluorene and 4-methylphenyl groups) with different degrees of electron-donating capability are incorporated, thus constructing the specific donor–donor–π–acceptor (D–D–π–A) system (C-CA, F-CA and I-3) and giving a systematic view of the absorption evolution. Through molecular engineering, their light-harvesting capabilities, energy levels and photovoltaic performances were studied. As expected, utilizing strong electron-donating carbazole unit as additional donor, the IPCE spectrum of DSSC based on C-CA is successfully broadened to NIR region on the premise of suitable LUMO level, with an extraordinarily high plateau in visible region till around 700 nm. In the system of C-CA and F-CA, the introduction of <I>n</I>-pentyl group in donor part of carbazole and fluorene unit has little effect on preventing the molecular π-aggregation due to the good co-planarity of π-linker (vinyl thiophene), suggesting that the most effective way to prevent π-aggregation is still the incorporation of long alkyl groups into planar π-linker segment. However, the introducing long alkyl group can effectively prevent the electron recombination between electrons in conduction band (CB) of TiO<SUB>2</SUB> and I<SUB>3</SUB><SUP>−</SUP> ions. Along with the preferable light-harvesting capability, C-CA presents excellent IPCE performance with a short-circuit photocurrent (<I>J</I><SUB>sc</SUB>) of 18.53 mA cm<SUP>−2</SUP>, an open-circuit photovoltage (<I>V</I><SUB>oc</SUB>) of 649 mV, a fill factor of 0.71, corresponding to a power conversion efficiency (<I>η</I>) of 8.49%. The internal relations between chemical structure and conversion efficiency provide a strategy for developing highly efficient organic sensitizers working in whole visible region with high photovoltaic performance.</P> <P>Graphic Abstract</P><P>Utilizing a carbazole unit as additional donor, the photoresponse of C-CA is broadened to the NIR region with a <I>η</I> of 8.49%. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2jm31704d'> </P>

Study of Ablation of Arc Contacts and Dynamic Contact Resistance in High Current Breaker

Licheng Xing,Xiaodong Zhang,Qingbin Tong,Gaoqi Xing 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.3

The fault of power circuit breaker (PCB) can lead very serious problems, especially in high voltage–power grid. The structure of contacts may be composed of main contacts and arc contacts, and this structure can avoid ablation of main contacts efciently. It is necessary to study the eroded state of the arc contacts and the dynamic contact resistance of arc contacts to estimate the operating state of the PCB. The wear of contacts contains arc ablation and mechanical wear through the characteristics of contacts. The arc ablation is caused by the high temperature of the arc and the mechanical wear is caused by the mechanical friction of static and movable contacts. Some experiments have been done under diferent size of contacts and diferent current through them. The results show that the mechanical wear increases, sometimes heavier, with the increase of the size of fxed contacts and the decrease of the size of movable ones because the force between fxed and movable contacts becomes larger. The results also show that the ablation of arc contacts increases with the increase of current, especially when the current is up to 20 kA. With the high temperature of arc reaching the hardness point of copper, the arc ablation and mechanical erosion are increasing obviously since the contacts become soft and easy to wear. Another result of the study shows it is important to choose the material of contacts and the force between movable and fxed contacts. The harder and less resistance the material of contacts, the better the operating situation. To monitor the operating situation of the breaker, the dynamic contact resistance was measured 25 times. As a result, the dynamic contact resistance is sensitive to the current and increasing with the increase of continuous experiment time because of the increasing of metal ions. Furthermore, if the SF6 in the PCB is changes, the dynamic contact resistance would be changed. The dynamic contact resistance in this experiment varies substantially within the range of 80–250 μΩ at the current of 20 kA.

Experimental Study on the Mechanical Performance of Grouted Specimen with Composite Ultrafine Cement Grouts

Maolin Tian,Lijun Han,Qingbin Meng 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.1

An experimental investigation was conducted in order to understand properties of grouts and grouted specimens. Three different cement types, i.e., composite ultrafine cement (CUC) that was independently developed, ultrafine cement (UC), and Portland cement (PC) were injected into broken red sandstone specimens on the basis of a self-designed grouting test equipment. After the grouting test, the effects on the mechanical behavior of grouted specimens were studied using an uniaxial compression test, macroscopic failure analysis and mesostructure analysis, as well as a comparison of the mechanical and structural properties of three types of grouted specimens was presented. The test results show that the compressive strength of grouted specimens improved in comparison to the rock residual strength, and the compressive strength of PC, UC and CUC grouted specimens increased by 23.0%, 59.6% and 101.5%, respectively. The failure modes of all grouted specimens were brittle failure, but only the CUC grouted specimen was failed along the new failure surfaces, indicating that CUC grouts can better bond the original cracks. The mesostructure characteristics obtained through the Digital 3D Video Microscope reveal the superior filling effect of the CUC grouts as well as verifying the macroscopic mechanical behavior.

Nonlinear Regression Analysis for Deep Rock Mass Parameters of the Hoek-Brown Failure Criterion Based on the Differential Evolution

Maolin Tian,Lijun Han,Qingbin Meng 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.8

The Hoek-Brown (H-B) failure criterion is an empirical failure criterion. The estimates of Hoek-Brown criterion parameters, such as the geological strength index (GSI) and the disturbance coefficient (D), are usually subjective. This paper focused on modifying the initial estimates of GSI and D to improve the accuracy of parameters. The nonlinear regression model (NLRM) of the Hoek-Brown failure criterion was proposed to analyze the rock parameters by using the sensitivity analysis and the displacement equation of the surrounding rocks. Then, a reasonable back analysis method was developed by introducing the differential evolution (DE), which was used to accurately obtain the parameters of the Hoek-Brown failure criterion in practical engineering. This method was successfully used to analyze the stability of the roadway in a deep coal mine. The results showed that the NLRM can better reflect the relationship between GSI, D, μ and the displacement of roadways, and the back analysis results are consistent with the filed monitoring results. This method can provide a helpful reference for modifying the influence of empirical and subjective factors on H-B parameters selection, and improving the accuracy of Hoek-Brown criterion parameters in the similar engineering applications.