Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)

Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7

Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax

Effect of Urbanization on Climate Change in Nanjing

Qi Ying Xiang,Yu Wenzheng,Shao Li,Chen Tianliang 보안공학연구지원센터 2014 International Journal of Smart Home Vol.8 No.1

Characterization of three serotonin receptors from the small white butterfly, <i>Pieris rapae</i>

Qi, Yi-xiang,Jin, Miao,Ni, Xu-yang,Ye, Gong-yin,Lee, Youngseok,Huang, Jia Elsevier 2017 Insect biochemistry and molecular biology Vol.87 No.-

<P><B>Abstract</B></P> <P>Serotonin (5-hydroxytryptamine, 5-HT) plays a key role in modulating diverse physiological processes and behaviors in both protostomes and deuterostomes. These functions are mediated through the binding of serotonin to its receptors, which are recognized as potential insecticide targets. We investigated the sequence, pharmacology and tissue distribution of three 5-HT receptors (Piera5-HT<SUB>1A</SUB>, Piera5-HT<SUB>1B</SUB>, Piera5-HT<SUB>7</SUB>) from the small white butterfly <I>Pieris rapae</I>, an important pest of cultivated cabbages and other mustard family crops. Activation of Piera5-HT<SUB>1A</SUB> or Piera5-HT<SUB>1B</SUB> by 5-HT inhibited the production of cAMP in a dose-dependent manner. Stimulation of Piera5-HT<SUB>7</SUB> with 5-HT increased cAMP level significantly. Surprisingly, with the exception of 5-methoxytryptamine, agonists including α-methylserotonin, 8-Hydroxy-DPAT and 5-carboxamidotryptamine activated these receptors poorly. The results are consistent with previous findings in <I>Manduca sexta</I>. All three receptors were blocked by methiothepin, but ketanserin and yohimbine were not effective. The selective mammalian 5-HT receptor antagonists SB 216641 and SB 269970 displayed potent inhibition effects on Piera5-HT<SUB>1B</SUB> and Piera5-HT<SUB>7</SUB> respectively. The results we achieved here indicate that the pharmacological properties of Lepidoptera 5-HT receptors are quite different from those in other insects and vertebrates and may contribute to development of new selective pesticides. This study offers important information on three 5-HT receptors from <I>P. rapae</I> that will facilitate further analysis of the functions of 5-HT receptors in insects.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Serotonin receptors Piera5-HT<SUB>1A</SUB>, Piera5-HT<SUB>1B</SUB>, and Piera5-HT<SUB>7</SUB> from <I>Pieris rapae</I> were cloned and examined. </LI> <LI> Upon serotonin activation, Piera5-HT<SUB>1A</SUB> and Piera5-HT<SUB>1B</SUB> inhibited cAMP production whereas Piera5-HT<SUB>7</SUB> induced it. </LI> <LI> All three receptors were poorly activated by serotoninergic agonists, indicating their distinct pharmacological properties. </LI> <LI> All three receptors were blocked by methiothepin; SB 216641 and SB 269970 inhibited Pr5-HT<SUB>1B</SUB> and Pr5-HT<SUB>7</SUB>, respectively. </LI> <LI> All three receptors were highly expressed in neuronal tissues while Pr5-HT<SUB>7</SUB> mRNA predominated in the gut. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Efficacy and Toxicity of Anti-VEGF Agents in Patients with Castration-Resistant Prostate Cancer: a Meta-analysis of Prospective Clinical Studies

Qi, Wei-Xiang,Fu, Shen,Zhang, Qing,Guo, Xiao-Mao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: Blocking angiogenesis by targeting vascular endothelial growth factor (VEGF) signaling pathway to inhibit tumor growth has proven to be successful in treating a variety of different metastatic tumor types, including kidney, colon, ovarian, and lung cancers, but its role in castration-resistant prostate cancer (CRPC) is still unknown. We here aimed to determine the efficacy and toxicities of anti-VEGF agents in patients with CRPC. Materials and Methods: The databases of PubMed, Web of Science and abstracts presented at the American Society of Clinical Oncology up to March 31, 2014 were searched for relevant articles. Pooled estimates of the objective response rate (ORR) and prostate-specific antigen (PSA) response rate (decline ${\geq}50%$) were calculated using the Comprehensive Meta-Analysis (version 2.2.064) software. Median weighted progression-free survival (PFS) and overall survival (OS) time for anti-VEGF monotherapy and anti-VEGF-based doublets were compared by two-sided Student's t test. Results: A total of 3,841 patients from 19 prospective studies (4 randomized controlled trials and 15 prospective nonrandomized cohort studies) were included for analysis. The pooled ORR was 12.4% with a higher response rate of 26.4% (95%CI, 13.6-44.9%) for anti-VEGF-based combinations vs. 6.7% (95%CI, 3.5-12.7%) for anti-VEGF alone (p=0.004). Similarly, the pooled PSA response rate was 32.4% with a higher PSA response rate of 52.8% (95%CI: 40.2-65.1%) for anti-VEGF-based combinations vs. 7.3% (95%CI, 3.6-14.2%) for anti-VEGF alone (p<0.001). Median PFS and OS were 6.9 and 22.1 months with weighted median PFS of 5.6 vs. 6.9 months (p<0.001) and weighted median OS of 13.1 vs. 22.1 months (p<0.001) for anti-VEGF monotherapy vs. anti-VEGF-based doublets. Conclusions: With available evidence, this pooled analysis indicates that anti-VEGF monotherapy has a modest effect in patients with CRPC, and clinical benefits gained from anti-VEGF-based doublets appear greater than anti-VEGF monotherapy.

Comparison of the Efficacy and Safety of EFGR Tyrosine Kinase Inhibitor Monotherapy with Standard Second-line Chemotherapy in Previously Treated Advanced Non-small-cell Lung Cancer: a Systematic Review and Meta-analysis

Qi, Wei-Xiang,Shen, Zan,Lin, Feng,Sun, Yuan-Jue,Min, Da-Liu,Tang, Li-Na,He, Ai-Na,Yao, Yang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Purpose: To compare the efficacy and safety of epidermal growth factor receptor tyrosine kinase inhibitormonotherapy (EFGR-TKIs: gefitinib or erlotinib) with standard second-line chemotherapy (single agent docetaxel or pemetrexed) in previously treated advanced non-small-cell lung cancer (NSCLC). Methods: We systematically searched for randomized clinical trials that compared EGFR-TKI monotherapy with standard second-line chemotherapy in previously treated advanced NSCLC. The end points were overall survival (OS), progression-free survival (PFS), overall response rate (ORR), 1-year survival rate (1-year SR) and grade 3 or 4 toxicities. The pooled hazard ratio (HR) or risk ratio (RR), with their corresponding 95% confidence intervals (CI) were calculated employing fixed- or random-effects models depending on the heterogeneity of the included trials. Results: Eight randomized controlled trials (totally 3218 patients) were eligible. Our meta-analysis results showed that EGFR-TKIs were comparable to standard second-line chemotherapy for advanced NSCLC in terms of overall survival (HR 1.00, 95%CI 0.92-1.10; p=0.943), progression-free survival (HR 0.90, 95%CI 0.75-1.08, P=0.258) and 1-year-survival rate (RR 0.97, 95%CI 0.87-1.08, P=0.619), and the overall response rate was higher in patients who receiving EGFR-TKIs(RR 1.50, 95%CI 1.22-1.83, P=0.000). Sub-group analysis demonstrated that EGFR-TKI monotherapy significantly improved PFS (HR 0.73, 95%CI: 0.55-0.97, p=0.03) and ORR (RR 1.96, 95%CI: 1.46-2.63, p=0.000) in East Asian patients, but it did not translate into increase in OS and 1-year SR. Furthermore, there were fewer incidences of grade 3 or 4 neutropenia, febrile neutropenia and neutrotoxicity in EGFR-TKI monotherapy group, excluding grade 3 or 4 rash. Conclusion: Both interventions had comparable efficacy as second-line treatments for patients with advanced NSCLC, and EGFR-TKI monotherapy was associated with less toxicity and better tolerability. Moreover, our data also demonstrated that EGFR-TKImonotherapy tended to be more effective in East Asian patients in terms of PFS and ORR compared with standard second-line chemotherapy. These results should help inform decisions about patient management and design of future trials.

ACCRETION-JET MODEL FOR THE HARD X-ray Γ - L_X CORRELATION IN BLACK HOLE X-ray BINARIES

YANG, QI-XIANG,XIE, FU-GUO,YUAN, FENG,ZDZIARSKI, ANDRZEJ A.,GIERLINSKI, MAREK,HO, LUIS C.,YU, ZHAOLONG The Korean Astronomical Society 2015 天文學論叢 Vol.30 No.2

In this work, we study the correlation between the photon index (${\Gamma}$) of the X-ray spectrum and the 2-10 keV X-ray luminosity ($L_X$) for black hole X-ray binaries (BHBs). The BHB sample is mainly from the quiescent, hard and intermediate states, with values of $L_X$ ranging from ${\sim}10^{30.5}$ to $10^{37.5}$ erg $s^{-1}$. We find that the photon index ${\Gamma}$ is positively or negatively correlated with the X-ray luminosity $L_X$, for $L_X$ above or below a critical value, ${\sim}10^{36.5}$ erg $s^{-1}$. This result is consistent with previous works. Moreover, when $L_X{\leq}{\sim}10^{33}$ erg $s^{-1}$, we found that the photon index is roughly independent of the X-ray luminosity. We interpret the above correlations in the framework of a coupled hot accretion flow - jet model. Besides, we also find that in the moderate-luminosity region, different sources may have different anti-correlation slopes, and we argue this diversity is caused by the different value of ${\delta}$, which describes the fraction of turbulent dissipation that directly heats electrons.

Causal Relations between Exposome and Stroke: A Mendelian Randomization Study

Hong-Qi Li,Yi-Wei Feng,Yu-Xiang Yang,Xin-Yi Leng,Prof Can Zhang,Shi-Dong Chen,Kevin Kuo,Shu-Yi Huang,Xue-Qing Zhang,Yi Dong,Xiang Han,Xin Cheng,Mei Cui,Lan Tan,Qiang Dong,Jin-Tai Yu 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

Background and Purpose To explore the causal relationships of elements of the exposome with ischemic stroke and its subtypes at the omics level and to provide evidence for stroke prevention. Methods We conducted a Mendelian randomization study between exposure and any ischemic stroke (AIS) and its subtypes (large-artery atherosclerotic disease [LAD], cardioembolic stroke [CE], and small vessel disease [SVD]). The exposure dataset was the UK Biobank involving 361,194 subjects, and the outcome dataset was the MEGASTROKE consortium including 52,000 participants. Results We found that higher blood pressure (BP) (systolic BP: odds ratio [OR], 1.02; 95% confidence interval [CI], 1.01 to 1.04; diastolic BP: OR, 1.03; 95% CI, 1.01 to 1.05; pulse pressure: OR, 1.03; 95% CI, 1.00 to 1.06), atrial fibrillation (OR, 1.18; 95% CI, 1.13 to 1.25), and diabetes (OR, 1.13; 95% CI, 1.07 to 1.18) were significantly associated with ischemic stroke. Importantly, higher education (OR, 0.69; 95% CI, 0.60 to 0.79) decreased the risk of ischemic stroke. Higher systolic BP (OR, 1.06; 95% CI, 1.02 to 1.10), pulse pressure (OR, 1.08; 95% CI, 1.02 to 1.14), diabetes (OR, 1.28; 95% CI, 1.13 to 1.45), and coronary artery disease (OR, 1.58; 95% CI, 1.25 to 2.00) could cause LAD. Atrial fibrillation could cause CE (OR, 1.90; 95% CI, 1.71 to 2.11). For SVD, higher systolic BP (OR, 1.04; 95% CI, 1.00 to 1.07), diastolic BP (OR, 1.06; 95% CI, 1.01 to 1.12), and diabetes (OR, 1.22; 95% CI, 1.10 to 1.36) were causal factors. Conclusions The study revealed elements of the exposome causally linked to ischemic stroke and its subtypes, including conventional causal risk factors and novel protective factors such as higher education.

佚名≪燕行錄≫作者及文學價値考述

칠영상 ( Yong Xiang Qi ) 고려대학교 중국학연구소 2007 中國學論叢 Vol.21 No.-

본 논문은 ≪燕行錄≫ 작자의 성명과 신분, 저작일정과 그 목적 등에 대하여 상세한 고증을 하여, 본 서의 작자는 金明遠이며,저작시기는 朝鮮 正祖八年 12월 12일부터 다음 해 4월 18일경임을 밝혔다. 본 使團은 朴明源의 率領을 축하하고 은혜에 감사하기 위한 것이다. 본 글에서는 金明遠 글에서의 淸 乾隆朝의 도시와 향촌경관이 당시 청나라 조정의 번성했던 시기를 진실하게 반영하고 있다고 평가하고 있다. 金氏는 중국 국학에 탁월하였고, 문학적 수양이 깊었으며, 경물을 논하거나, 고금의 이해 이외에도 개인의 정감적인 묘사와 평론 등의 방면에서도 단연 우수했다. 그의 ≪燕行錄≫은 보기 드물게 아름답고 청아한 수품문집이라 할 만 하다. ≪燕行錄≫又稱≪朝天錄≫,是中國明淸時期朝鮮使臣往來燕京(今北京)等地時根據路途聞見所記載下來的紀行錄. 因爲是朝鮮王朝重要官員與知識分子用他們獨特的眼光與感知,對明淸時期中國的自然風貌、人文景觀以及朝政國情等的記錄與論述,所以這些≪燕行錄≫對硏究當時的中朝關係、中朝文化交流與中國社會以及朝鮮社會等方面,都有著極其重要的價値.筆者在閱讀大量同類書籍時,對乾隆年間佚名所撰≪燕行錄≫發生了濃厚的興趣,現就此書作者及其相關問題討論如下.

淸代學術拾零 (20條)

칠영상 ( Yong Xiang Qi ) 중국어문연구회 2007 中國語文論叢 Vol.0 No.33

한어역주본(漢語譯註本)《을병조천록(乙丙朝天錄)》 규오(糾誤)

칠영상 ( Yong Xiang Qi ),려춘연 ( Chun Yan Lu ) 中國語文硏究會 2009 中國語文論叢 Vol.0 No.40