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Anna Ratz-Łyko,Anna Herman,Jacek Arct,Katarzyna Pytkowska 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
The antioxidant and antimicrobial activities ofOenothera biennis, Borago officinalis, and Nigella sativaseedcake extracts were determined. Seedcakes weresubjected to extraction using 50% ethanol (v/v) during 48 hat a solvent temperature of 50oC. The total polyphenol andflavonoid contents were assayed using UV spectrophotometryand the antioxidant activities were determinedusing DPPH and ABTS testing methods. Extracts werealso analyzed for polyphenol content using HPLC. In vitroantimicrobial activities were evaluated using the discdiffusion method and resazurin testing. The ranking of thepolyphenol contents and the antioxidant activities ofexamined seedcake extracts was O. bienis>B.officinalis>N. sativa. The highest antimicrobial activity in both testswas for N. sativa seedcake extracts against Pseudomonasaeruginosa (MIC=300 mg/mL), Escherichia coli (MIC=300 mg/mL), and Staphylococcus aureus (MIC=700 mg/mL).