Research on Application of Blockchain Technology In the International Transaction of Steel Industry

Puyu Wang,Linke Zhou,Ying Huang 한국관세학회 2019 한국관세학회 학술대회 Vol.2019 No.6

Letter of Credit (L/C) is a primary way of payment in an international steel trade. However, due to the risks of documentary fraud, it is a great challenge to the confidence of L/C service in the complex trade environment. Firstly, this paper summarizes the methods and specific characteristics of L/C service in the steel trade, and also realizes the risks of confidence in the process of international transaction. Then, based on underlying technology and characteristics of blockchain technology, a new solution will be conducted related to L/C service to solve the weakness of L/C, including the documentary fraud, long payment cycle, repeated operations and high additional costs.

The Hyponormal Toeplitz operators on the vector valued Bergman space

Yufeng Lu,Puyu Cui,Yanyue Shi 대한수학회 2014 대한수학회보 Vol.51 No.1

In this paper, we give a necessary and sufficient condition for the hyponormality of the block Toeplitz operators TΦ, where Φ = F +G∗, F(z), G(z) are some matrix valued polynomials on the vector valued Bergman space L2 a(D, Cn). We also show some necessary conditions for the hyponormality of TF+G* with F + G∗ 2 h∞ ⓧ Mn×n on L2 a(D, Cn). In this paper, we give a necessary and sufficient condition for the hyponormality of the block Toeplitz operators TΦ, where Φ = F +G∗, F(z), G(z) are some matrix valued polynomials on the vector valued Bergman space L2 a(D, Cn). We also show some necessary conditions for the hyponormality of TF+G* with F + G∗ ∈ h∞ ⓧ Mn×n on L2 a(D, Cn).

Analysis of Operation Characteristics of a Diesel Generator Set with Constant‑Power Pulsed Load

Meng Shi,Puyu Wang,Jinquan Wang,Haichao Li,Ye Xu,Kefeng Huang,Jianke Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.6

To improve the power quality of a diesel generator set with a pulsed load in a microgrid, this paper studied the operation characteristics of the system and proposed pulsed load conditions for an actual project based on test data. For a low average pulse power and a high peak power, a new mathematical model of the pulsed load in diferent operation modes was proposed. Then, a simulation model of the microgrid was established in MATLAB/Simulink, and the operation of the system was studied considering changes in various pulsed load parameters (the peak power Pk, output duty cycle D and switching period Ts). It was verifed by tests that the simulation model is efective and can refect the operation of the system. Based on the simulation model, the operation characteristics of the system with a bivariate pulsed load were studied, and qualifcation conditions for the diesel generator set in an actual project were proposed.

THE HYPONORMAL TOEPLITZ OPERATORS ON THE VECTOR VALUED BERGMAN SPACE

Lu, Yufeng,Cui, Puyu,Shi, Yanyue Korean Mathematical Society 2014 대한수학회보 Vol.51 No.1

In this paper, we give a necessary and sufficient condition for the hyponormality of the block Toeplitz operators $T_{\Phi}$, where ${\Phi}$ = $F+G^*$, F(z), G(z) are some matrix valued polynomials on the vector valued Bergman space $L^2_a(\mathbb{D},\mathbb{C}^n)$. We also show some necessary conditions for the hyponormality of $T_{F+G^*}$ with $F+G^*{\in}h^{\infty}{\otimes}M_{n{\times}n}$ on $L^2_a(\mathbb{D},\mathbb{C}^n)$.

In-silico annotation of the chemical composition of Tibetan tea and its mechanism on antioxidant and lipid-lowering in mice

Wang Ning,Li Linman,Zhang Puyu,Mehmood Muhammad Aamer,Lan Chaohua,Gan Tian,Li Zaixin,Zhang Zhi,Xu Kewei,Mo Shan,Xia Gang,Wu Tao,Zhu Hui 한국영양학회 2023 Nutrition Research and Practice Vol.17 No.4

BACKGROUND/OBJECTIVES: Tibetan tea is a kind of dark tea, due to the inherent complexity of natural products, the chemical composition and beneficial effects of Tibetan tea are not fully understood. The objective of this study was to unravel the composition of Tibetan tea using knowledge-guided multilayer network (KGMN) techniques and explore its potential antioxidant and hypolipidemic mechanisms in mice. MATERIALS/METHODS: The C57BL/6J mice were continuously gavaged with Tibetan tea extract (T group), green tea extract (G group) and ddH2O (H group) for 15 days. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in mice was detected. Transcriptome sequencing technology was used to investigate the molecular mechanisms underlying the antioxidant and lipid-lowering effects of Tibetan tea in mice. Furthermore, the expression levels of liver antioxidant and lipid metabolism related genes in various groups were detected by the real-time quantitative polymerase chain reaction (qPCR) method. RESULTS: The results showed that a total of 42 flavonoids are provisionally annotated in Tibetan tea using KGMN strategies. Tibetan tea significantly reduced body weight gain and increased T-AOC and SOD activities in mice compared with the H group. Based on the results of transcriptome and qPCR, it was confirmed that Tibetan tea could play a key role in antioxidant and lipid lowering by regulating oxidative stress and lipid metabolism related pathways such as insulin resistance, P53 signaling pathway, insulin signaling pathway, fatty acid elongation and fatty acid metabolism. CONCLUSIONS: This study was the first to use computational tools to deeply explore the composition of Tibetan tea and revealed its potential antioxidant and hypolipidemic mechanisms, and it provides new insights into the composition and bioactivity of Tibetan tea.

LncRNA MALAT1 Depressed Chemo-Sensitivity of NSCLC Cells through Directly Functioning on miR-197-3p/p120 Catenin Axis

Tian Yang,Hong Li,Tianjun Chen,Hui Ren,Puyu Shi,Mingwei Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.3

This study was aimed to explore if lncRNA MALAT1 would modify chemo-resistance of non-small cell lung cancer (NSCLC) cells by regulating miR-197-3p and p120 catenin (p120-ctn). Within this investigation, we totally recruited 326 lung cancer patients, and purchased 4 NSCLC cell lines of A549, H1299, SPC-A-1 and H460. Moreover, cisplatin, adriamycin, gefitinib and paclitaxel were arranged as chemotherapies, and half maximal inhibitory concentration (IC50) values were calculated to evaluate the chemo-resistance of the cells. Furthermore, mice models of NSCLC were also established to assess the impacts of MALAT1, miR-197-3p and p120-ctn on tumor growth. Our results indicated that MALAT1 and miR-197-3p were both over-expressed within NSCLC tissues and cells, when compared with normal tissues and cells (P < 0.05). The A549, H460, SPC-A-1 and SPC-A-1 displayed maximum resistances to cisplatin (IC50 = 15.70 μg/ml), adriamycin (IC50 = 5.58 μg/ml), gefitinib (96.82 μmol/L) and paclitaxel (141.97 nmol/L). Over-expression of MALAT1 and miR-197-3p, or under-expression of p120-ctn were associated with promoted viability and growth of the cancer cells (P < 0.05), and they could significantly strengthen the chemo-resistance of cancer cells (P < 0.05). MALAT1 Wt or p120-ctn Wt co-transfected with miR-197-3p mimic was observed with significantly reduced luciferase activity within NSCLC cells (P < 0.05). Finally, the NSCLC mice models were observed with larger tumor size and weight under circumstances of over-expressed MALAT1 and miR-197-3p, or under-expressed p120-ctn (P < 0.05). In conclusion, MALAT1 could alter chemo-resistance of NSCLC cells by targeting miR-197-3p and regulating p120-ctn expression, which might assist in improvement of chemo-therapies for NSCLC.

Polystyrene-b-poly(oligo(ethylene oxide) Monomethyl Ether Methacrylate)-b-polystyrene Triblock Copolymers as Potential Carriers for Hydrophobic Drugs

You, Qianqian,Chang, Haibo,Guo, Qipeng,Zhang, Yudong,Zhang, Puyu Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

A simple and effective method is introduced to synthesize a series of polystyrene-b-poly(oligo(ethylene oxide) monomethyl ether methacrylate)-b-polystyrene (PSt-b-POEOMA-b-PSt) triblock copolymers. The structures of PSt-b-POEOMA-b-PSt copolymers were characterized by Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance ($^1H$ NMR) spectroscopy. The molecular weight and molecular weight distribution of the copolymer were measured by gel permeation chromatography (GPC). Furthermore, the self-assembling and drug-loaded behaviours of three different ratios of PSt-b-POEOMA-b-PSt were studied. These copolymers could readily self-assemble into micelles in aqueous solution. The vitamin E-loaded copolymer micelles were produced by the dialysis method. The micelle size and core-shell structure of the block copolymer micelles and the drug-loaded micelles were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The thermal properties of the copolymer micelles before and after drug-loaded were investigated by different scanning calorimetry (DSC). The results show that the micelle size is slightly increased with increasing the content of hydrophobic segments and the micelles are still core-shell spherical structures after drug-loaded. Moreover, the glass transition temperature (Tg) of polystyrene is reduced after the drug loaded. The drug loading content (DLC) of the copolymer micelles is 70%-80% by ultraviolet (UV) photolithography analysis. These properties indicate the micelles self-assembled from PSt-b-POEOMA-b-PSt copolymers would have potential as carriers for the encapsulation of hydrophobic drugs.

Polystyrene-b-poly(oligo(ethylene oxide) Monomethyl Ether Methacrylate)-b-polystyrene Triblock Copolymers as Potential Carriers for Hydrophobic Drugs

Qianqian You,Haibo Chang,Qipeng Guo,Yudong Zhang,Puyu Zhang 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.2

A simple and effective method is introduced to synthesize a series of polystyrene-b-poly(oligo(ethylene oxide) monomethyl ether methacrylate)-b-polystyrene (PSt-b-POEOMA-b-PSt) triblock copolymers. The structures of PSt-b-POEOMA-b-PSt copolymers were characterized by Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (1H NMR) spectroscopy. The molecular weight and molecular weight distribution of the copolymer were measured by gel permeation chromatography (GPC). Furthermore£¨the self-assembling and drug-loaded behaviours of three different ratios of PSt-b-POEOMA-b-PSt were studied. These copolymers could readily self-assemble into micelles in aqueous solution. The vitamin E-loaded copolymer micelles were produced by the dialysis method. The micelle size and core-shell structure of the block copolymer micelles and the drug-loaded micelles were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). The thermal properties of the copolymer micelles before and after drug-loaded were investigated by different scanning calorimetry (DSC). The results show that the micelle size is slightly increased with increasing the content of hydrophobic segments and the micelles are still core-shell spherical structures after drug-loaded. Moreover, the glass transition temperature (Tg) of polystyrene is reduced after the drug loaded. The drug loading content (DLC) of the copolymer micelles is 70%-80% by ultraviolet (UV) photolithography analysis. These properties indicate the micelles self-assembled from PSt-b- POEOMA-b- PSt copolymers would have potential as carriers for the encapsulation of hydrophobic drugs.

LncRNA MALAT1 Depressed Chemo-Sensitivity of NSCLC Cells through Directly Functioning on miR-197-3p/p120 Catenin Axis

Yang, Tian,Li, Hong,Chen, Tianjun,Ren, Hui,Shi, Puyu,Chen, Mingwei Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.3

This study was aimed to explore if lncRNA MALAT1 would modify chemo-resistance of non-small cell lung cancer (NSCLC) cells by regulating miR-197-3p and p120 catenin (p120-ctn). Within this investigation, we totally recruited 326 lung cancer patients, and purchased 4 NSCLC cell lines of A549, H1299, SPC-A-1 and H460. Moreover, cisplatin, adriamycin, gefitinib and paclitaxel were arranged as chemotherapies, and half maximal inhibitory concentration (IC50) values were calculated to evaluate the chemo-resistance of the cells. Furthermore, mice models of NSCLC were also established to assess the impacts of MALAT1, miR-197-3p and p120-ctn on tumor growth. Our results indicated that MALAT1 and miR-197-3p were both over-expressed within NSCLC tissues and cells, when compared with normal tissues and cells (P < 0.05). The A549, H460, SPC-A-1 and SPC-A-1 displayed maximum resistances to cisplatin ($IC50=15.70{\mu}g/ml$), adriamycin ($IC50=5.58{\mu}g/ml$), gefitinib ($96.82{\mu}mol/L$) and paclitaxel (141.97 nmol/L). Over-expression of MALAT1 and miR-197-3p, or under-expression of p120-ctn were associated with promoted viability and growth of the cancer cells (P < 0.05), and they could significantly strengthen the chemo-resistance of cancer cells (P < 0.05). MALAT1 Wt or p120-ctn Wt co-transfected with miR-197-3p mimic was observed with significantly reduced luciferase activity within NSCLC cells (P < 0.05). Finally, the NSCLC mice models were observed with larger tumor size and weight under circumstances of over-expressed MALAT1 and miR-197-3p, or under-expressed p120-ctn (P < 0.05). In conclusion, MALAT1 could alter chemo-resistance of NSCLC cells by targeting miR-197-3p and regulating p120-ctn expression, which might assist in improvement of chemo-therapies for NSCLC.