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Purevjargal Naidansuren,Kwan-Sik Min 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2
We prepared the polyclonal antibody anti-20α-hydroxysteroid dehydrogenase (anti-20α-HSD) against the recombinant full-length protein bovine 20α-HSD in Escherichia coli. The specificity of anti-20α-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine 20α-HSD and bovine placental tissues. According to western blot analysis, anti-20α-HSD specifically recognizes the 37-kDa protein bovine 20α-HSD. The protein is not present in untransfected CHO cells. Anti-20α-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine 20α-HSD protein in the cultured luteal cells during the estrous cycle later.
Expression and Characterization of Monkey 20a-HSD Gene
Purevjargal Naidnsuren,Sung-Jo Yun,Jong-Ju Park,Won-Gun Noh,Chae-Won Park,Tsevelmaa Nanjidsuren,Young-Sil Kang,Seongsoo Hwang,Jong-Taek Yoon,Sang-Rae Lee,Kyu-Tae Chang,Kwan Sik-Min 한국동물번식학회 2009 Reproductive & Developmental Biology(Supplement) Vol.33 No.2s
Purevjargal Naidansuren,Kwan-Sik Min 한국동물생명공학회(구 한국동물번식학회) 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
We prepared the polyclonal antibody anti-20α-hydroxysteroid dehydrogenase (anti-20α-HSD) against the recombinant full-length protein bovine 20α-HSD in Escherichia coli. The specificity of anti-20α-HSD was demonstrated using Chinese hamster ovary (CHO) cells transfected with recombinant bovine 20α-HSD and bovine placental tissues. According to western blot analysis, anti-20α-HSD specifically recognizes the 37-kDa protein bovine 20α-HSD. The protein is not present in untransfected CHO cells. Anti-20α-HSD also recognizes a specific protein in the ovaries and placenta of other animals. Immunostaining was used to detect expression of bovine 20α-HSD protein in the cultured luteal cells during the estrous cycle later.
Expression and Characterization of Monkey 20a-HSD Gene
Purevjargal Naidnsuren,Sung-Jo Yun,Jong-Ju Park,Won-Gun Noh,Chae-Won Park,Tsevelmaa Nanjidsuren,Young-Sil Kang,Seongsoo Hwang,Jong-Taek Yoon,Sang-Rae Lee,Kyu-Tae Chang,Kwan Sik-Min 한국동물생명공학회(구 한국동물번식학회) 2009 Reproductive & developmental biology Vol.33 No.2