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Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review
Paes Sabrina Moreira,Pupo Yasmine Mendes,Cavenago Bruno Cavalini,Fonseca-Silva Thiago,Santos Carolina Carvalho de Oliveira 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.2
Objectives: The aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery. Materials and Methods: This systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study. Results: The search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis. Conclusions: The cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.
Effect of official judo matches on handgrip strength and perceptual responses
Rafael Lima Kons,Juliano Dal Pupo,Jonathan Ache-Dias,Thyago Garcia,Romário Rodrigues da Silva,Luiz Felipe Guarise Katicips,Daniele Detanico 한국운동재활학회 2018 JER Vol.14 No.1
This study aimed to verify the effect of judo matches on handgrip strength and perceptual responses during an official tournament in medalists and nonmedalists. Thirty-four male judo athletes participated in an offi-cial judo tournament. Before the first match and immediately after each match, maximum isometric handgrip strength and rate of perceived ex-ertion overall and in specific areas were assessed. Analysis of variance for repeated measures was used to compare variables before the first match and after each match, and t-test was used to compare medalists and nonmedalists with the level of significance set at 5%. Also, effect size (ES) analysis was used. The results showed decrease in handgrip strength in both hands from the third match (P<0.05). The rate of per-ceived exertion (RPE) increased from the first match and remained high over the subsequent matches (P<0.001). A very large effect for nonmed-alist group (ES=3.44) and large effect for medalist group (ES=1.94) was found in the third match compared to prematch. Forearm and fingers were the body regions most cited by athletes in both groups. We con-cluded that an official judo competition induced significant drop in hand-grip strength from the third match and increased the RPE from first match. Medalists seem to have better recovery after the third match compared to nonmedalists.
In situ screening of 3-arylcoumarin derivatives reveals new inhibitors of mast cell degranulation
Marcela de Souza Santos,Maria Perpe´tua Freire de Morais Del Lama,Laila Aparecida Deliberto,Fla´vio da Silva Emery,Moˆnica Tallarico Pupo,Rose Mary Zumstein Georgetto Naal 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.6
Due to the severity and high prevalence ofallergic diseases, there is growing interest in the developmentof inhibitors of such conditions. 3-Arylcoumarinderivatives emerge as promising compounds for thetreatment of allergic disorders, in particular due to theirclose structural similarity to flavonoids, whose anti-allergicactivity has been extensively reported. The aim of this workwas to perform a screening of a set of 3-arylcoumarins aspotential inhibitors of mast cell degranulation, a key eventfor the development of allergic reactions. For that purpose, itwas utilized a biosensor model based on mast cells, whosein vitro assay allows for such screening, in a high throughputfashion, and also permits bringing to attention some coumarinstructural features that are important for their biologicalactivity. The mast cell-based biosensor was shown todiscriminate, with high sensitivity and reproducibility,between coumarins that did not affect or caused differentdegrees of inhibition of degranulation. Among activecoumarins, some substituents could be accounted for theirinhibitory activity, such as the hydroxylation of positions 6and 20 of 3-phenylcoumarins, in addition to catechol, aminoand thiophene moieties. In summary, 3-arylcoumarins couldbe suggested as potential candidates for the developmentof new anti-allergic drugs.