Hydrogeochemical signatures origin of a karst geothermal reservoir–the Sinian Dengying Formation in northern Guizhou, China

Pu Liu,Zhengshan Chen,Ganlu Wang,Tie Mao,Chao Li,Yanan Zhou,Hong Xie,Tong Xiang 한국지질과학협의회 2024 Geosciences Journal Vol.28 No.1

The karst geothermal reservoir, Sinian Dengying Formation was investigated from the hydrogeochemical perspective. Integrated techniques including XRD + SEM, isotopes, saturation index and PHREEQC modeling were applied. With 37.3–72 ?, the targeted waters indicate low-mediate temperature geothermal reservoir. The CO2 concentration in the geothermal waters varies from 1.2 mg/L to 52.42 mg/L, and the log(PCO2) value ranges from −2.53 to −1.29, indicating an open carbonate system. A further D-18O stable isotope analysis addresses the geothermal waters are replenished by meteoric water from +815.52 m to +1427.78 m. Strong water-rock interactions are indicated by apparent 18O shift. The dominating hydrochemical type is SO4·HCO3-Ca·Mg with some Cl-Na. The CO2-TDS-SI-Carbonates comprehensive analysis illustrate the origin of Ca2+, Mg2+, and HCO3−, mainly from dolomite. The correlation of 18O-SO42-SI_Gypsum gives sound evidence for the processes of gypsum dissolution. Three inverse models were established and the results showed, that geothermal waters are controlled by two major factors: 1) the adjacent location to salt basin geological profiles with halite dissolution; and 2) the clay minerals such as Na-montmorillonite causing cation exchanges between Na+ and Ca2+ in the geothermal waters, resulting in excess of Na in relation to Cl concentrations.

Variants on ESR1 and their Association with Prostate Cancer Risk: A Meta-analysis

Ding, Xiang,Cui, Feng-Mei,Xu, Song-Tao,Pu, Jin-Xian,Huang, Yu-Hua,Zhang, Jiang-Lei,Wei, Xue-Dong,Hou, Jian-Quan,Yan, Chun-Yin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: Epidemiological studies evaluating the association of two variants rs9340799 and rs2234693 on estrogen receptor 1 (ESR1) with prostate risk have generated inconsistent results. Methods: A meta-analysis was here conducted to systematically evaluate the relationship of these two variants with prostate cancer susceptibility. Results: For rs9340799, heterozygosity of T/C carriers showed a significant increased prostate cancer risk with a pooled odds ratio (OR) of 1.34 (95% CI = 1.06-1.69) while homozygote C/C carriers showed an increased but not statistically significant association with prostate cancer risk (pooled OR = 1.29, 95% CI = 0.94-1.79). Compared to the homozygous TT carriers, the allele C carriers showed a 31% increased risk for prostate cancer (pooled OR = 1.31, 95% CI = 1.06-1.63). No significant association between the rs2234693 and prostate cancer risk was found with the pooled OR of 1.15 (95% CI = 0.97-1.39, T/C and C/C vs. T/T) under the dominant genetic model. Compared to the homozygote T/T carriers, the heterozygous T/C carriers did not show any significantly different risk of prostate cancer (pooled OR = 1.13, 95% CI = 0.94-1.36) and the homozygous C/C carriers also did not show a significant change for prostate cancer risk compared to the wide-type T/T carriers (pooled OR = 1.26, 95% CI = 0.98-1.62). Conclusion: These data suggested that variant rs9340799, but not rs2234693, on ESR1 confers an elevated risk of prostate cancer.

Meta Analysis of Association of the IL-17F rs763780T>C Gene Polymorphism with Cancer Risk

Chen, Xiang-Jun,Zhou, Tao-You,Chen, Min,Pu, Dan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Purpose: To investigate the association of IL-17F rs763780T>C with cancer risk. Materials and Methods: We searched the Cochrane Central Library, PubMed, MEDLINE, EMBASE, CNKI (China National Knowledge Infrastructure) and WangFang databases until May 2014 for a meta-analysis conducted using RevMan 5.2 software. Results: A total of ten papers were included into this meta analysis, involving 3, 336 cases and 4, 217 healthy people. There were no significant differences on association of IL-17F rs763780T>C polymorphism with cancer risk except in the CC vs TT genetic model. Although the the risk in the gastric cancer group is higher than that in control group, there were no significant differences on the association of IL-17F rs763780T>C polymorphism with other cancers. Conclusions: Our meta analysis reveal the IL-17A rs763780T>C gene polymorphism is involved in risk of gastric cancer but not other tumor types.

Correlations Between Serum IL33 and Tumor Development: a Meta-analysis

Chen, Xiang-Jun,Huang, Ying-De,Li, Nian,Chen, Min,Liu, Fang,Pu, Dan,Zhou, Tao-You Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Background: Interleukin-33 (IL-33) has recently been implicated in tumor development. Methods: Data was obtained from PubMed, EMBASE, Clinical trial, Cochrane Library, Web of Science, CNKI and Wanfang databases. After quality assessment and data extraction, a meta-analysis was performed using Review Manager 5.2 software. Results: There were eight documents included in this meta-analysis. The results showed IL33 levels to be higher in tumor patients than that in health people, but no correlations tumor stage, metastasis and survival time of tumor patients were evident. Conclusion: IL33 may be useful as an alarm factor in tumor detection and prognosis.

A bibliometric analysis of worldwide educational artificial intelligence research development in recent twenty years

Song Pu,Wang Xiang 서울대학교 교육연구소 2020 Asia Pacific Education Review Vol.21 No.3

Educational artificial intelligence (EAI) refers to the use of artificial intelligence (AI) to support personalized and automated feedback and guidance in the educational field. Inevitably, it serves as a more important part of the educational system in the coming years. However, novel development in this field has been inadequately reviewed and conceptualized in a visualized, objective and comprehensive way. In this view, a bibliometric analysis was conducted to obtain an overview of its trends from publication outputs, countries’ cooperation, cluster analysis, and research evolution. Around 8660 Scopus-published articles from 2000 to 2019 were gathered for analysis using CiteSpace and Alluvial generator. In the study, a growing interest in EAI research and deepening cooperation among countries was first identified, entailing favorable conditions for promoting globalization in this aspect. Afterward, five core clusters were established for the intellectual structure of EAI, including intelligent tutoring system, learning system, student, labeled training data, and pedagogy. The development of EAI research was further conceptualized as follows: (a) technological foundation; (b) technological breakthrough; (c) intelligent application; and (d) symbiotic integration. Finally, three prospective directions for future EAI research were suggested.

Ultrasound Score to Select Subcentimeter-sized Thyroid Nodules Requiring Ultrasound-guided Fine Needle Aspiration Biopsy in Eastern China

Cheng, Pu,Chen, En-Dong,Zheng, Hua-Min,He, Qiu-Xiang,Li, Quan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8

Ultrasound-guided fine needle aspiration biopsy (FNAB) is a costly diagnostic item with a low yield in identifying the tiny proportion of nodules that actually represent malignant disease. Our aim through this study was to obtain an ultrasound (US) score for selecting subcentimeter-sized thyroid nodules requiring FNAB in eastern China. Some 248 patients for a total of 270 thyroid nodules less than 1 cm in diameter underwent FNAB and subsequent surgery from January 2006 to March 2012 at our hospital. The clinicopathological and US data from all the nodules were analyzed retrospectively. An US score was developed on the basis of independent predictive factors for malignancy. Irregular shape, hypoechogenicity, no well-defined margin, presence of calcifications and ratio between antero-posterior and transversal diameters (AP/TR) ${\geq}1$ were independent predictive factors for malignancy on logistic regression analysis. US score were statistically significant, with ${\leq}2$ favoring benignancy with an 80.3% sensitivity and a 72.7% specificity. US score is useful for differentiating between malignant and benign subcentimeter-sized thyroid nodules. We suggest FNAB for nodules when the US score is higher than 2.

Expression of hPOT1 in HeLa Cells and the Probability of Gene Variation of hpot1 Exon14 in Endometrial Cancer are Much Higher than in Other Cancers

Liu, Fei,Pu, Xiao-Yun,Huang, Shao-Guang,Xiang, Gui-Ming,Jiang, Dong-Neng,Hou, Gou,Huang, Di-Nan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

To investigate the expression of hPOT1 in the HeLa cell line and screen point mutations of hpot1 in different tumor tissues a two step osmotic method was used to extract nuclear proteins. EMSA was performed to determine the expression of hPOT1 in the HeLa cell line. PCR was also employed to amplify the exon14 sequence of the hpot1 gene in various of cancer tissues. A SV gel and PCR clean-up system was performed to enrich PCR products. DNAStar was used to analyse the exon14 sequence of the hpot1 gene. hPOT1 was expressed in the HeLa cell line and the signal was gradually enhanced as the amount of extracted nuclear proteins increased. The DNA fragment of exon14 of hpot1 was successfully amplified in the HeLa cell line and all cancer tissues, point mutations being observed in 2 out of 3 cases of endometrial cancer (66.7%) despite the hpot1 sequence being highly conserved. However, the sequence of hpot1 exon14 do not demonstrate point mutations in most cancer tissues. Since hPOT1 was expressed in HeLa cell and the probability of gene point variants was obviously higher in endometrial cancer than other cancers, it may be involved in the pathogenesis of gynecological cancers, especially in cervix and endometrium.

A New Practical System for Evaluating the Pharmacological Properties of Uricase as a Potential Drug for Hyperuricemia

Juan Feng,Xiang Li,Xiaolan Yang,Chun Zhang,Yonghua Yuan,Jun Pu,Yunsheng Zhao,Yanling Xie,Huidong Yuan,Youquan Bu,Fei Liao 대한약학회 2010 Archives of Pharmacal Research Vol.33 No.11

The use of uricase-deficient mammals to screen formulations of engineered uricases as potential drugs for hyperuricemia involves heavy costs and presents a technical bottleneck. Herein, a new practical system was investigated to evaluate the pharmacological significance of a bacterial uricase based on its ability to eliminate uric acid in plasma in vitro, its pharmacokinetics in vivo in healthy rats, and the modeled pharmacodynamics in vivo. This uricase, before and after modification with the monomethyl ether of poly(ethylene glycol)-5000, effectively eliminated uric acid in vitro in rabbit plasma, but its action was susceptible to xanthine inhibition. After intravenous injection of the modified uricase without purification, a bi-exponential model fit well to uricase activities in vivo in the plasma of healthy rats; the half-life of the modified uricase was estimated without interference from the unmodified uricase leftover in the sample and was nearly 100-fold longer than that of the unmodified uricase. Using a model of the elimination of uric acid in vivo taking into account of uricase pharmacokinetics and xanthine inhibition, modeled pharmacodynamics supported that the half-life of uricase and its susceptibility to xanthine are crucial for the pharmacological significance of uricase. Hence,this practical system is desirable for doing preliminary screening of formulations of engineered uricases as potential drugs for hyperuricemia.

De novo assembled transcriptome of horned gall aphid, Schlechtendalia chinensis Bell, suggest changes in functional gene expression during host alternation

Si‐Liang Wang,Zi-Xiang YANG,Pu YANG,Chuan Xi Zhang 한국곤충학회 2016 Entomological Research Vol.46 No.5

The horned gall aphid, Schlechtendalia chinensis Bell, is the most economically valuable galling aphid. It accounts for approximately 75% of Chinese gallnut production, which is important for medical and chemical purposes. The horned gall aphid feeds on the Chinese sumac tree, Rhus chinensis, during the summer, induces galls, and overwinters on the moss Plagiomnium spp. However, the sequence information of S. chinensis is limited, and the functional gene expression changes during host alternation remain obscure. In this study, the transcriptome of the horned gall aphid was sequenced using high‐throughput sequencing technology. Approximately 49 million reads were obtained and were assembled into 99 366 transcripts and 47 400 unigenes. Annotation was performed using a BLAST search against primary databases such as NCBI nr and Swiss‐Prot, as well as enrichment analysis by GO, KOG and KEGG. Differentially expressed genes between spring and autumn migrant aphids were compared, and the expression levels of many genes related to detoxification, digestion and other functional genes were found to have changed, reflecting the adaptation ability of horned gall aphids in different seasons and hosts. Functional genes in wax biosynthesis were upregulated in spring migrant aphids. This study analyzed the expression profile difference between spring and autumn migrant aphids to approach the real functional genes change during host alternation. The transcriptome provides a basis to understanding the functional genes during gall aphid host alternation and the related physiological and molecular characteristics.

Hydrothermal Synthesis, Crystal Structure and EPR Property of Tetranuclear Copper(II) Cluster [Cu4OCl6(C14H12N2)4]

Fang-fang Jian*,Pu-su Zhao,Huan-xiang Wang,Lu-de Lu 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.5