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( Pritish Chandra Pal ),( Ashish Bali ),( Ramanarayana Boyapati ),( Sangita Show ),( Kanikanti Siva Tejaswi ),( Sourabh Khandelwal ) 영남대학교 의과대학 2022 Yeungnam University Journal of Medicine Vol.39 No.4
Background: The combined use of biomaterials for regeneration may have great biological relevance. This study aimed to compare the regenerative potential of biphasic calcium phosphate (BCP) alone and with growth factor enamel matrix derivatives (EMDs) for the regeneration of intrabony defects at 1 year. Methods: This randomized controlled trial included 40 sites in 29 patients with stage II/III periodontitis and 2/3 wall intrabony defects that were treated with BCP alone (control group) or a combination of BCP and EMD (test group). BCP alloplastic bone grafts provide better bio-absorbability and accelerate bone formation. EMDs are commercially available amelogenins. Mean values and standard deviations were calculated for the following parameters: plaque index (PI), papillary bleeding index (PBI), vertical probing pocket depth (V-PPD), vertical clinical attachment level (V-CAL), and radiographic defect depth (RDD). Student paired and unpaired t-tests were used to compare the data from baseline to 12 months for each group and between the groups, respectively. The results were considered statistically significant at p< 0.05. Results: At 12 months, the PI and PBI scores of the control and test groups were not significantly different (p >0.05). The mean V-PPD difference, V-CAL gain, and RDD difference were statistically significant in both groups at 12 months (p<0.001 for all parameters). Intergroup comparisons showed that the mean V-PPD reduction (2.13±1.35 mm), V-CAL gain (2.53±1.2 mm), and RDD fill (1.33±1.0 mm) were statistically significant between the groups at 12 months (p<0.001 for all parameters). Conclusion: BCP and EMDs combination is a promising modality for the regeneration of intrabony defects.
Epigenomic Hard Drive Imprinting: A Hidden Code Beyond the Biological Death of Cancer Patients
Pritish Nilendu,Nilesh Kumar Sharma 대한암예방학회 2017 Journal of cancer prevention Vol.22 No.4
Several genetic and epigenetic theories have been suggested to explain the intricacies of life and death. However, several questions remain unsettled regarding cellular death events, particularly of living tissue in the case of cancer patients, such as the fate and adaptation of cancer cells after biological death. It is possible that cancer cells can display the intent to communicate with the external environment after biological death by means of molecular, genetic, and epigenetic pathways. Whether these cancer cells contain special information in the form of coding that may help them survive beyond the biological death of cancer patients is unknown. To understand these queries in the cancer field, we hypothesize the epigenomic hard drive (EHD) as a cellular component to record and store global epigenetic events in cancerous and non-cancerous tissues of cancer patients. This mini-review presents the novel concept of EHD that is reinforced with the existing knowledge of genetic and epigenetic events in cancer. Further, we summarize the EHD understanding that may impart much potential and interest for basic and clinical scientists to unravel mechanisms of carcinogenesis, therapeutic markers, and differential drug responses.
Unnati Pitale,Pritish Chandra Pal,Ramanarayana Boyapati,Ashish Bali,Manish Varma,Shaleen Khetarpal 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.6
Objectives: Loss of the interdental papilla is multi-factorial and creates a multitude of problems. Autogenous connective tissue/biomaterial-based regen-eration has been attempted for decades to reconstitute the black space created due to the loss of papilla. The aim of this present study was to regenerate papillary recession defects using an amnion-chorion membrane (ACM) allograft and to evaluate the clinical outcome up to six months postoperatively. Materials and Methods: Twenty patients with 25 Nordland and Tarnow’s Class I/II interdental papillary recession defects were treated with ACM and coronal advancement of the gingivo-papillary unit via a semilunar incision on the labial aspect followed by a sulcular incision in the area of inter-est. A photographic image analysis was carried out using the GNU Image Manipulation software program from the baseline to three and six months postoperatively. The black triangle height (BTH) and the black triangle width (BTW) were calculated using the pixel size and were then converted into millimeters. The mean and standard deviation values were determined at baseline and then again at three and six months postoperatively. The prob-ability values (P˂0.05 and P≤0.01) were considered statistically significant and highly significant, respectively. An analysis of variance and post hoc Bonferroni test were carried out to compare the mean values. Results: Our evaluation of the BTH and BTW showed a statistically and highly significant difference from the baseline until both three and six months postoperatively (P=0.01). A post hoc Bonferroni test disclosed a statistically significant variance from the baseline until three and six months postoperatively (P˂0.05) and a non-significant difference from three to six months after the procedure (P≥0.05). Conclusion: An ACM allograft in conjunction with a coronally advanced flap could be a suitable minimally invasive alternative for papillary regeneration.
Raj, Utkarsh,Kumar, Himansu,Gupta, Saurabh,Varadwaj, Pritish Kumar Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
Background: The human protein methyl-transferase DOT1L catalyzes the methylation of histone H3 on lysine 79 (H3K79) at homeobox genes and is also involved in a number of significant processes ranging from gene expression to DNA-damage response and cell cycle progression. Inhibition of DOT1L activity by shRNA or small-molecule inhibitors has been established to prevent proliferation of various MLL-rearranged leukemia cells in vitro, establishing DOT1L an attractive therapeutic target for mixed lineage leukemia (MLL). Most of the drugs currently in use for the MLL treatment are reported to have low efficacy, hence this study focused on various natural compounds which exhibit minimal toxic effects and high efficacy for the target receptor. Materials and Methods: Structures of human protein methyl-transferase DOT1L and natural compound databases were downloaded from various sources. Virtual screening, molecular docking, dynamics simulation and drug likeness studies were performed for those natural compounds to evaluate and analyze their anti-cancer activity. Results: The top five screened compounds possessing good binding affinity were identified as potential high affinity inhibitors against DOT1L's active site. The top ranking molecule amongst the screened ligands had a Glide g-score of -10.940 kcal/mol and Glide e-model score of -86.011 with 5 hydrogen bonds and 12 hydrophobic contacts. This ligand's behaviour also showed consistency during the simulation of protein-ligand complex for 20000 ps, which is indicative of its stability in the receptor pocket. Conclusions: The ligand obtained out of this screening study can be considered as a potential inhibitor for DOT1L and further can be treated as a lead for the drug designing pipeline.
( Veerendra Chadachan ),( Yi Lin Teh ),( Pritish Kumar Gehlot ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Carbimazole is the standard antithyroid drug treatment for patients with thyrotoxicosis, especially in young women of reproductive age. A serious side effect of antithyroid drug use, however, is agranulocytosis. Management of a severely thyrotoxic patient who has antithyroid drug induced agranulocytosis is challenging. Methods: We report here a young female who presented with fever, cough and severe neutropenia after she was started on carbimazole for thyrotoxicosis. She complainted of intermittent episodes of palpitations and diarrhoea. Physical examination revealed a diffuse goitre and multiple oral ulcers. She was tachycardic, but hemodynamically stable. Rest of the physical examination was unremarkable. Investigations revealed she had severe neutropenia, and markedly elevated free T4 and T3 hormone levels. Results: She was started on broad spectrum antibiotics. The administration of granulocyte colony stimulating factor resulted in an improvement in the white blood cell count only after seven days. With carbimazole being stopped, her thyrotoxicosis went on worsening, and she had features of high output cardiac failure. She underwent 3 cycles of plasmapheresis, with which her thyroid function tests stabilised. However, she developed hypotension and hypoxia during the fourth cycle of plasmapheresis due to cardiac failure. Once her thyroid function tests stabilised, she was transferred to receive radio-active iodine. Conclusions: Antithyroid drug-induced agranulocytosis is a potentially lethal condition but is completely reversible when recognised early and when prompt treatment is offered. Management of such a patient who is severely thyrotoxic is very difficult. We will discuss here the literature review on carbimazole-induced agranulocytosis and illustrate the challenges we face in managing such situations and discuss what are the strategies in managing such patients.
Saurabh Gupta,Vikas Gupta,Vishal Singh,Pritish Kumar Varadwaj 한국유전학회 2018 Genes & Genomics Vol.40 No.9
A wide range of plant species growth influenced when they exposed to solar UV-B radiation. Leaves of the plant are highly affected by UV-B radiation lead to the reduction in the growth of the plant. Current work demonstrates the comparative transcriptional changes and visible symptoms occurred in the maize leaf growth zone (GZ). Primary objective of this study was to identify differentially expressed genes (DEGs) responsible for leaf growth and their association in the transcriptional regulatory network under UV-B stress. Whole transcriptomic data was analysed and the quality check was tested for each sample and further genome-wide mapping and DEGs were performed. Gene Ontology (GO) based functional annotation, associated transcriptional networks and molecular pathways were annotated. Reduction in cell production due to UV-B stress causes a decrease in leaf’s length and size was observed. Further, the specific role of the DEGs, in UV-B signalling pathways and other molecular functions responsible for leaf cell death was discovered. Results also infer that the major changes occurred in the cell cycle, transcriptional regulation, post-transcriptional modification, phytohormones, flavonoids biosynthesis, and chromatin remodeling. UV-B signalling pathways and the transcriptional regulatory networks infer the different molecular steps along with downstream transcriptional and post-transcriptional control of metabolic enzymes used in long-term memory adoption and attainment resistance to UV-B stress identified. Effects of UV-B radiation on leaf growth was noted in this study. UV-B stress response genes and associated transcriptional regulatory networks were identified, can be used in developing the marker assist UB-B stress tolerant genotypes of the maize.