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Harvesting of Chlorella vulgaris by using chitosan as bioflocculant
( Prabuddha Gupta ),최희정,이승목 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Recently, there has been increasing interest for production of biofuels from algae biomass as raw material. Owning to small size and low concentration of microalgae, harvesting of microalgae is major challenge and requires high-energy input for harvesting biomass. In order to make commercial microalgal biodiesel production economically feasible, cost effective dewatering techniques are necessary. In this study, we evaluated the potential of chitosan as a flocculant for harvesting microalgae. Chitosan was found to be efficient flocculant for harvesting freshwater microalgae Chlorella vulgaris. Chitosan was effective in pH ranging from 4 to 8. The optimal chitosan concentration required to effect maximum flocculation depended on the pH of solution, concentration of algal cells, ionic strength, and culture volume. For effective flocculation, lowest amount of chitosan concentration (7 mg) was obtained with 90% recovery efficiency at pH 6.3.
Prabuddha Manjula,조성현,서국진,서동원,이준헌 한국가금학회 2018 韓國家禽學會誌 Vol.45 No.4
TBC1D1 gene has known functional effects on body energy homeostasis and glucose uptake pathway in skeletal muscle tissue. This biological function is reported to have significant effects on traits of growth and meat quality in chicken. In this study, we focused on two single nucleotide polymorphisms (SNPs) (g.70179137A>G and g.70175861T>C) identified through SNP annotation information of Korean native chicken and previous literature for TBC1D1 in chicken. Association of SNPs in TBC1D1 with growth and serum clinical-chemical traits were evaluated. A total of 584 male and female birds from five Korean native chicken lines were used in the study. The SNP1 (g.70179137A>G) is located in intron 11 and SNP2 (g.70175861T>C) is a non-synonymous missense mutation in exon 10, responsible for the amino acid change from Methionine to Valine. The A allele of SNP1 and T allele of SNP2 had the highest allele frequencies. Both SNPs indicated moderate polymorphism information content values (0.25<PIC<0.5). Association results shown that SNP1 was significantly associated with BW00, BW20, GR14-16 and carcass weight (P<0.05). The AA genotype had higher value for all these traits except BW00. Whereas, genotype GG have lower values for BW20, GR14-16, GR18-20 and carcass weight. The sGOT level was significant with SNP1 but not with the SNP2. The GG genotype had the highest sGOT value and AG reported the lowest. BW20 was significantly associated with SNP2 (P<0.05). The body weight and carcass weight values of CC and TC genotypes were higher than those of TT genotype.
Comparative Study on Growth Patterns of 25 Commercial Strains of Korean Native Chicken
Prabuddha Manjula,Hee-Bok Park,Jaehong Yoo,Samiru Wickramasuriya,Dong-Won Seo,Nu-Ri Choi,Chong Dae Kim,Bo-Seok Kang,Ki-Seok Oh,Sea-Hwan Sohn,Jung-Min Heo,Jun-Heon Lee 한국가금학회 2016 韓國家禽學會誌 Vol.43 No.1
Prediction of growth patterns of commercial chicken strains is important. It can provide visual assessment of growth as function of time and prediction body weight (BW) at a specific age. The aim of current study is to compare the three nonlinear functions (i.e., Logistic, Gompertz, and von Betalanffy) for modeling the growth of twenty five commercial Korean native chicken (KNC) strains reared under a battery cage system until 32 weeks of age and to evaluate the three models with regard to their ability to describe the relationship between BW and age. A clear difference in growth pattern among 25 strains were observed and classified in to the groups according to their growth patterns. The highest and lowest estimated values for asymptotic body weight (C) for 3H and 5W were given by von Bertalanffy and Logistic model 4629.7 g for 2197.8 g respectively. The highest estimated parameter for maturating rate (b) was given by Logistic model 0.249 corresponds to the 2F and lowest in von Bertalanffy model 0.094 for 4Y. According to the coefficient of determination (R2) and mean square of error (MSE), Gompertz and von Bertalanffy models were suitable to describe the growth of Korean native chicken. Moreover, von Bertalannfy model was well described the most of KNC growth with biologically meaningful parameter compared to Gompertz model.
Enhancing efficiency of constructed wetlands by using biochar
( Prabuddha Gupta ),안태웅,이승목 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
Constructed wetlands are established efficient technologies and provide sustainable solution for wastewater treatment. SimilarlyBiochar, which is an organic material, produced by means of pyrolysis, offers simple and low cost techniques to treat water and reduce carbon footprint. Combining both of these technologies can greatly augment the efficiency of the system. The objective of this study was to evaluate the efficiency of constructed wetlands by combining it with biochar. Horizontal wetland beds were prepared using gravels, biochar and cultivatedwith Cannaspecies. These beds were saturated with wastewater with a retention time of 5 days. Performance parameters were assessed by estimating reduction of COD, TN, NH<sub>3</sub>, NO<sub>3</sub>-N, TP, PO<sub>4</sub>-P between controlled and experimental wetland beds. The study revealed that wetland with biochar was more efficient as compared to wetland with gravels alone.
( Prabuddha Gupta ),최희정,이승목 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
The challenges in overcoming the nutrient sourcing and biomass production can be achieved by employing wastewaters as nutrients by means of mixotrophy. In this study, nutrient removal from municipal wastewater by Chlorella vulgaris andNannochloropsisoculata was investigated by using mixotrophic mode of nutrition using glycerol as carbon source. The study comprises of mixotrophic growth of C. vulgaris and N. oculata under different concentration of glycerol (0.2 to 2.0 g/L) supplemented with municipal wastewater. Performance parameters were assessed by estimating reduction of TN, TP, biomass growth, chlorophyll content and lipid yield. Mixotrophic culture of C. vulgaris showed an increase of 2.42 fold of biomass productivity as compared to the photoautotrophic culture. Similarly highest TN removal of 88.36% and TP removal 47.48% was observed inC. vulgarisculture with 2 g L<sup>-1</sup> glycerol.
( Prabuddha Gupta ),최희정,이승목 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Mixotrophic cultivation is a significant method to enhance microalgal biomass. In current study, mixotrophic growth condition of microalgae Chlorella vulgaris was evaluated under various carbon sources (glucose, acetate, and glycerol) in wastewater. Under optimized condition i.e. 5 g/L glucose C. vulgaris produced 2.04 g/L dry cell weight containing 17.3% total lipid and 39.68% carbohydrate. Addition of glucose enhances the rapid cell growth and helps in waste water nutrient removal TN (58%) and TP (74%). This study shows potential application of mixotrophically grown microalgae for bioethanol and biodiesel production feed stock along with nutrient removal from wastewater.
( Prabuddha Gupta ),최희정,이승목 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.1
Among numerous challenges faced in the commercial cultivation of microalgae, providing a cost effective water and nutrients source is crucial. Our study aimed to utilize the municipal wastewater as nutrient feedstock for mixotrophic microalgal cultivation by using glycerol as carbon source.In present study, nutrient removal from municipal wastewater by Chlorella vulgaris and Nannochloropsis oculata was investigated by using mixotrophic cultivation with glycerol (0 to 5 g L-1). Performance parameters were assessed by estimating the removal of total nitrogen, total phosphorus, biomass growth, chlorophyll content, lipid yield and fatty acids. With the addition of 2 g L-1glycerol, a maximum biomass productivity of 0.056 g L-1d-1 was achieved in the mixotrophic culture of C. vulgaris within 12 days. The mixotrophic culture showed a 30 fold increase in biomass productivity compared to the photoautotrophic culture. However, the highest total nitrogen removal (80.62%) and total phosphate removal (60.72%) was observed in the N. oculata culture supplemented with 3 and 5 g L-1 glycerol respectively. These results suggest that mixotrophic cultivation using glycerol offers great potential in the production of renewable biomass, waste water treatment and consequent production of high-value microalgal oil.