Letters to the Editor : A Case of Blastic Plasmacytoid Dendritic Cell Neoplasm

( Teresa Pinto Almeida ),( Iolanda Fernandes ),( Madalena Sanches ),( Catarina Lau ),( Margarida Lima ),( Rosario Alves ),( Manuela Selores ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.2

Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis

Leal Pinto Sandra Milena,Muehlmann Luis Alexandre,Ojeda Lucía Liliana Mantilla,Vera Arias Angélica María,Cordero Martha Viviana Roa,Santos María de Fátima Menezes Almeida,Azevedo Ricardo Bentes,Rivero 대한감염학회 2021 Infection and Chemotherapy Vol.53 No.2

Background: Photodynamic therapy (PDT) using chloroaluminium phthalocyanine (ClAlPc) and paromomycin sulfate (PM) can be effective against New World Leishmania species involved in cutaneous leishmaniasis (CL). The aim of this study is to assay the skin permeation and the antileishmanial effects of a nanoemulsion (NE) containing both ClAlPc and PM in experimental CL by Leishmania (Viannia) braziliensis. Material and Methods: Cremophor ELP/castor oil-based NEs were prepared by a low-energy method and characterized for their physicochemical parameters. The NEs were used to deliver both ClAlPc and PM to leishmania cells. The in vitro toxicity of NEs were tested in vitro against L. (V.) braziliensis and THP-1 cells. The in vivo toxicity was assessed in non-infected BALB/c mice. Ex-vivo permeation and retention studies using healthy mice skin were also conducted. Finally, the in vivo activity of NE-PM+ClAlPc after PDT was tested in BALB/c mice infected with parasites. Results: NEs are colloidally stable with average droplet diameter of 30 nm, polydispersity index (PDI) below 0.2, and zeta potential near zero. Both promastigotes and intracellular amastigotes treated with NE-PM, NE-ClAlPc and NE-PM+ClAlPc were inhibited at >50%, >95%, >88%, respectively, after PDT with a phototoxic index (PI) >1.2. No skin ClAlPc permeation was observed. In contrast, PM skin permeation was 80-fold higher using PMloaded NE formulation in comparison to aqueous PM solution. Topical treatment with NE formulations showed no signs of local toxicity or genotoxicity. In addition, concentrations of PM between 27.3 - 292.5 μM/25 mg of tissue were detected in different organs. In vivo, the NE-PM+ClAlPc treatment did not reduce skin lesions. Conclusion: The Cremophor ELP/castor oil NE formulation increases the permeation of PM through the skin and can be used to co-deliver PM plus ClAlPc for combined PDT protocols. However, the lack of efficacy in the in vivo model evidences that the therapeutical scheme has to be improved.

Facial and occlusal esthetic improvements of an adult skeletal Class III malocclusion using surgical, orthodontic, and implant treatment

Mauricio de Almeida Cardoso,Rafael Scaf de Molon,Erica Dorigatti de Avila,Fabio Pinto Guedes,Valter Antonio Ban Battilani F,Leopoldino Capelozza Filho,Marcio Aurelio Correa,Hugo Nary Filho 대한치과교정학회 2016 대한치과교정학회지 Vol.46 No.1

The aim of this clinical report is to describe the complex treatment of an adult Class III malocclusion patient who was disappointed with the outcome of a previous oral rehabilitation. Interdisciplinary treatment planning was performed with a primary indication for implant removal because of marginal bone loss and gingival recession, followed by orthodontic and surgical procedures to correct the esthetics and skeletal malocclusion. The comprehensive treatment approach included: (1) implant removal in the area of the central incisors; (2) combined orthodontic decompensation with mesial displacement and forced extrusion of the lateral incisors; (3) extraction of the lateral incisors and placement of new implants corresponding to the central incisors, which received provisional crowns; (4) orthognathic surgery for maxillary advancement to improve occlusal and facial relationships; and finally, (5) orthodontic refinement followed by definitive prosthetic rehabilitation of the maxillary central incisors and reshaping of the adjacent teeth. At the three-year follow-up, clinical and radiographic examinations showed successful replacement of the central incisors and improved skeletal and esthetic appearances. Moreover, a Class II molar relationship was obtained with an ideal overbite, overjet, and intercuspation. In conclusion, we report the successful esthetic anterior rehabilitation of a complex case in which interdisciplinary treatment planning improved facial harmony, provided gingival architecture with sufficient width and thickness, and improved smile esthetics, resulting in enhanced patient comfort and satisfaction. This clinical case report might be useful to improve facial esthetics and occlusion in patients with dentoalveolar and skeletal defects.

Poster Session : PS 0605 ; Pulmonology ; How Far Can We Go without Empiric Treatment?

( Lise Brosseron ),( Sara Almeida Pinto ),( Jose Ribeiro Almeida ),( Tiago Pereira ),( Judite Quintas ),( Vitor Paixao Dias ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Tuberculosis is a known cause of fever of unknown origin. Nowadays most authors consider mycobacterium tuberculosis isolation essential prior to treatment. Case Report: A 63 year-old women was admitted to our department with vespertine fever, night sweats, anorexia and weight loss in the past 4 months. She had a history of childhood pulmonary tuberculosis, with sequelar bronchiectasis. Besides paleness and low BMI no other relevant physical fi ndings were observed. Laboratory evaluation revealed anemia and elevation of CRP and ESR. Thorax-CT showed bronchiectasis on the upper and inferior right lobe. Bronchoalveolar lavage cultures were negative to bacteria, mycobacteria and fungus as well as M.tuberculosis complex DNA. Despite multiple antibiotic courses she maintained intermittent pyrexia. After excluding most relevant causes of fever of unknown origin, a PET scan confi rmed exclusive involvement of the right superior pulmonary lobe. In the second bronchofi broscopy signifi cant bronchial hemorrhage was visible, so catheter embolization of the right bronchial artery was performed. An arterio-arterial subclavian-pulmonary fi stula was detected. Two days later multiple infra and supratentorial intracerebral hemorrhages were diagnosed. In the following week she had two ischemic strokes affecting the right and left middle cerebral artery territory. Transcranial Doppler showed moderate right-to-left shunt. All those events were suggestive of embolic etiology. Despite all negative cultural and DNA results, attending to typical manifestations, exclusion of other foci and to this rapid deterioration, empiric treatment was started with isoniazid, rifampin, ethambutol and pyrazinamide. One month after treatment initiation, persistent apyrexia was obtained with normalization of all infi ammatory parameters. In the last 3 months good evolution of pulmonary lesions and signifi cant improvement of her neurologic state were observed. Conclusions: We present a bizarre case of tuberculosis with catastrophic consequences, which demonstrates that empiric treatment still has its place in modern medicine.

Effect of Babassu Mesocarp As a Food Supplement During Resistance Training

Maísa Carvalho Rezende Soares,Mayara Cristina Pinto Silva,Francisco de Assis da Silva Almeida-Junior,Johnny Ramos Nascimento,Flavia Raquel Fernandes Nascimento,Rosane Nassar Meireles Guerra 한국식품영양과학회 2021 Journal of medicinal food Vol.24 No.4

The population widely uses babassu mesocarp (Attalea speciosa) as food and medicine. This study evaluated the use of babassu mesocarp as a food supplement during resistance training (RT). Male Swiss mice, 60 days old (weight 35–40 g), were divided into four groups (n = 8): control, untreated and untrained; babassu (babassu aqueous extract [BAE]), treated orally with aqueous extract of babassu mesocarp (25 mg/kg), five times a week, for 8 weeks; training (RT), submitted to RT consisting of stair climbing with progressive loads; and resistance training treated with babassu aqueous extract (RTBAE): RT and treatment with BAE. After 8 weeks, we analyzed the biochemistry of serum, the immunological, and histological parameters. The RT group showed maximum strength after the second week. A reduction in body weight, retroperitoneal and interstitial fat deposits, and activated helper T lymphocytes (TCD4+ CD69+) occurred in RT and RTBAE groups. The RTBAE group showed increased levels of aspartate aminotransferase, alanine aminotransferase, and macrophage and helper T lymphocyte count, whereas a reduction occurred in triglyceride levels and the total number of lymphocytes. Supplementation with BAE always reduced cholesterol and the population of activated macrophages but increased activated B lymphocytes and interleukin-6 levels. The combination of supplementation and RT resulted in a decreased production of tumor necrosis factor-α. We propose the use of babassu mesocarp as a food supplement during exercise because of its immunomodulatory effect on lymphocyte and macrophage populations and cytokine production. The additional impact on the control of cholesterol and triglyceride levels suggests its use, particularly for the treatment of dyslipidemias.

Plaster body wrap: effects on abdominal fat

Juliana Santos Moreira,Ana Sofia Carneiro Pinto de Melo,Andreia Noites,Miriam Faria Couto,Cristina Argel de Melo,Nuno Carvalho Freire de Almeida Adubeiro 한국한의학연구원 2013 Integrative Medicine Research Vol.2 No.4

Background: Abdominal fat is associated with metabolic disorders, leading to cardiovascular risk factors and numerous diseases. This study aimed to analyze the effect of plaster body wrap in combination with aerobic exercise on abdominal fat. Methods: Nineteen female volunteers were randomly divided into intervention group (IG; n = 10) performing aerobic exercise with plaster body wrap, and control group (CG; n = 9) performing only exercise. Subcutaneous and visceral fat were measured using ultrasound; subcutaneous fat was also estimated on analysis of skinfolds and abdominal perimeters. Results: At the end of the 10-sessions protocol, the IG demonstrated a significant decrease (p ≤ 0.05) in subcutaneous fat at the left anterior superior iliac spine (ASIS) level and in iliac crest perimeter measurements. A large intervention effect size strength (0.80) was found in subcutaneous fat below the navel and a moderate effect size strength on the vertical abdominal skinfold (0.62) and the perimeter of the most prominent abdominal point (0.57). Comparing the initial and final data of each group, the IG showed a significant decrease in numerous variables including visceral and subcutaneous fat above and below the navel measured by ultrasound (p ≤ 0.05). Conclusion: Plaster body wrap in combination with aerobic exercise seems to be effective for abdominal fat reduction.

Pectin Solubility and Water Relations during Vase Life of Cut Flowers

Maria Helena Teixeira Gomes,Susana Maria Pinto de Carvalho,Domingos Paulo Ferreira de Almeida 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.4

Pectic polymers are major components of primary cell walls having a high water-binding ability. Pectin levels and solubility were quantified in stems and petals of rose (Rosa hybrida L.), chrysanthemum (Chrysanthemum morifolium Ramat.), carnation (Dianthus caryophyllus L.), and snapdragon (Anthirrhinum majus L.) and related to the rate of solution uptake and to the rate of fresh weight increase during the vase life of these cut flowers. Total pectins in stems ranged from 176 ㎍ㆍ㎎?¹ in chrysanthemum to 203 ㎍ㆍ㎎?¹ in carnation. Water soluble pectin accounted for only 1.9 to 3.6% of total stem pectins, in rose and snapdragon, respectively, whereas chelator-soluble pectins ranged from 3.8% of total in chrysanthemum to 10.1% in snapdragon. Petals of each species had a higher proportion of water- and chelator-soluble pectins than stems. Water uptake rate during vase life was 0.16 gㆍg?¹ㆍday?¹ for carnation, 0.35 gㆍg?¹ㆍday?¹ for chrysanthemum, 0.52 gㆍg?¹ㆍday?¹ for rose, and 0.62 gㆍg?¹ㆍday?¹ for snapdragon. In the absence of salts, maximum fresh weight increase in relation to the initial value varied from 15% in carnation to 33% in chrysanthemum. Addition of KCl or CaCl₂ to the vase solution depressed the uptake rate but prolonged the period of increasing fresh weight and delayed the fresh weight decline. Significant positive linear relationships were found between the solubility of stem pectins and the rate of fresh weight increase in the four cut flowers and between the solution uptake rate and the amount of CDTA-soluble pectins present in the petals. These results provide correlative evidence for a role of pectins in the water relations of cut flowers.

Adhesive systems applied to dentin substrate under electric current; systematic review

Maciel Carolina Menezes,Souto Tatiane Cristina Vieira,Pinto Bárbara de Almeida,Silva-Concilio Laís Regiane,Baroudi Kusai,Vitti Rafael Pino 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4

Objectives The purpose of this systematic review was to collect and discuss the technique of adhesive systems application on dentin substrate under electric current. Materials and Methods The first search strategy was based on data available at PubMed, LILACS, Scielo, Scopus, and Cochrane Library, using a combination of descriptors such as “dentin bond agents OR adhesive system AND electric current OR electrobond” or “dentin bonding agents OR dentin bonding agent application OR adhesive system AND electric current OR electrobond”, with no limit regarding the publication year. The second search strategy was based on the articles' references found previously. An additional search strategy was applied that concerned the proposed theme in the SBU-UNICAMP (Unicamp's Library System Institutional Repository). Results Twelve studies published between 2006 and 2020 were found. The analyses of the selected studies showed that the use of electric current during adhesive systems application on dentin, whether conventional or self-conditioning, increases resinous monomer infiltration in the dentin substrate, which improves the hybridization processes and the bond strength of the restorative material to dentin. Conclusions Despite the favorable results related to the use of this technique, there is still no specific protocol for the application of adhesive systems under electric current. Objectives The purpose of this systematic review was to collect and discuss the technique of adhesive systems application on dentin substrate under electric current. Materials and Methods The first search strategy was based on data available at PubMed, LILACS, Scielo, Scopus, and Cochrane Library, using a combination of descriptors such as “dentin bond agents OR adhesive system AND electric current OR electrobond” or “dentin bonding agents OR dentin bonding agent application OR adhesive system AND electric current OR electrobond”, with no limit regarding the publication year. The second search strategy was based on the articles' references found previously. An additional search strategy was applied that concerned the proposed theme in the SBU-UNICAMP (Unicamp's Library System Institutional Repository). Results Twelve studies published between 2006 and 2020 were found. The analyses of the selected studies showed that the use of electric current during adhesive systems application on dentin, whether conventional or self-conditioning, increases resinous monomer infiltration in the dentin substrate, which improves the hybridization processes and the bond strength of the restorative material to dentin. Conclusions Despite the favorable results related to the use of this technique, there is still no specific protocol for the application of adhesive systems under electric current.

Physical-chemical and biological characterization of different preparations of equine chorionic gonadotropin

Rafael Herrera Alvarez,Fabio Luis Nogueira Natal,Maria Teresa Carvalho Pinto Ribela,Beatriz Elane de Almeida,João Ezequiel de Oliveira,Paolo Bartolini 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4

Ovarian stimulation with commercial preparations of equine chorionic gonadotropin (eCG) produces extremely variable responses indomestic animals, ranging from excessive stimulation to practically no stimulation, when applied on the basis of their declared unitage. Thisstudy was conducted to analyze four commercial preparations from different manufacturers via reversed-phase HPLC (RP-HPLC) incomparison with a reference preparation and an official International Standard from the World Health Organization. The peaks obtained bythis qualitative and quantitative physical–chemical analysis were compared using an in vivo bioassay based on the ovarian weight gain ofprepubertal female rats. The RP-HPLC data showed one or two peaks close to a main peak (tR = 27.9 min), which were related to the in vivobioactivity. Commercial preparations that have this altered peak showed very little or no in vivo activity, as demonstrated by rat ovarian weightand in peripubertal gilts induced to ovulate. Overall, these findings indicate that RP-HPLC can be a rapid and reliable tool to reveal changesin the physicochemical profile of commercial eCG that is apparently related to decreased biological activity of this hormone.

Effect of sodium bicarbonate supplementation on two different performance indicators in sports: a systematic review with meta-analysis

( Ramon Souza Lino ),( Laura Souza Lagares ),( Caio Victor Coutinho Oliveira ),( Ciro Oliveira Queiroz ),( Lélia Lessa Teixeira Pinto ),( Luiz Alberto Bastos Almeida ),( Eric Simas Bonfim ),( Clarcson 한국운동영양학회 2021 Physical Activity and Nutrition (Phys Act Nutr) Vol.25 No.1

[Purpose] Sodium bicarbonate shows ergogenic potential in physical exercise and sports activities, although there is no strong evidence which performance markers show the greatest benefit from this supplement. This study evaluated the effects of sodium bicarbonate supplementation on time trial performance and time to exhaustion in athletes and sports practitioners. [Methods] A systematic review was conducted using three databases, including 17 clinical trials. Among these clinical trials, 11 were considered eligible for the meta-analysis according to the criteria for the assessment of methodological quality using the PEDro Scale. Time to exhaustion was assessed in six studies, while time trial performance was evaluated in five studies. [Results] A significant beneficial effect of supplementation on time to exhaustion was found in a random effects model (1.48; 95% confidence interval [CI], 0.49 to 2.48). There was no significant effect of supplementation on time trial performance in a fixed effects model (slope = -0.75; 95% CI, -2.04 to 0.55) relative to a placebo group. [Conclusion] Sodium bicarbonate has the potential to improve sports performance in general, especially in terms of time to exhaustion.