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( Hua Li ),( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( Xue Ji Han ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1
Background: Opa interacting protein 5 (OIP5) encodes a 25- kDa protein with a coiled-coil domain. Previous studies havedemonstrated the elevated expression of OIP5 mRNA in human gastric and colorectal carcinomas, but no report has functionally clarified the significance of OIP5 expression in human hepatocarcinogenesis and indicated its potential as a therapeutic target. The aim of study is to examine biological role of OIP5 in hepatocarcinogenesis. Methods: OIP5 mRNA expression was confirmed by Realtime- PCR analysis. OIP5 protein expression was investigated in HCC tissue and hepatoma cells by immunoblot and immunohistochemistry analysis. Cellular localization of OIP5 was detected by immunofluorescence. For the parallel determination of the relative levels of protein phosphorylation and mitogenactivated protein pinases (MAPKs) was experimented by the human phosphor-kinase array and (Phospho-MAPK) Array. Results: OIP5 abundantly expressed in tumor compared with non-tumor HCC tissues. OIP5 was localized in the nucleus and cytoplasm. Immunohistochemistry staining demonstrated that OIP5 was absent in normal liver, present in early and advanced tumours. Knockdown of OIP5 by shRNA specific to resulted in growth inhibition of hepatoma cell lines. Colony generation detected that OIP5 overexpression was approximate 2-fold more colony number. The knockdown of OIP5 resulted in decrease of soft agar colony generation. Phospho-AKT (s437) level was increased with OIP5 overexpression. OIP5 upregulated in mitotic cell whereas OIP5 did not change in interphase. Conclusions: OIP5 may involved in hepatocarcinogenesis through Akt activation and tumor cell cycle progression.
Pei-Qing Cong,Eun-Sook Song,Eui-Sook Kim,Zhao-Hua Li,Yong-Hua Zhang,Young-Joo Yi,Chang-Sik Park 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2
This study was designed to evaluate effects of BSA, PVA, gonadotropins and follicle shell during IVM of porcine oocytes and subsequent development to the blastocyst stage after IVF. Cumulus oocyte complexes (COCs) were cultured in TCM-199 media containing 4 mg/ml BSA and 1 mg/ml PVA during IVM for 44 hr. To compare the effect of gonadotropins on oocyte maturation, COCs were cultured with FSH+LH, FSH, LH and FSH-LH-free media during IVM, respectively. Also, different number of follicle shells (0, 2, 4 and 6) was used to examine whether the presence of follicle shell in culture medium affects oocyte maturation. The percentages of fertilization and blastocyst formation, respectively, were higher in the medium containing the PVA (49.0 and 17.9%) than those containing the BSA (40.0 and 12.2%). Significantly higher rates of MII oocytes were in the presence of FSH+LH and FSH (88.6 and 85.1%) compared to other treatments (64.0 and 53.4% at LH and FSH-LH-free media). Co-culture with inverted follicle shells in 2 ml maturation medium enhanced the developmental competence of porcine oocytes. In conclusion, PVA could be used as a macromolecules instead of BSA, and FSH and follicle shell played important roles in maturation of porcine oocytes.
Pei-QingCong,Eun-Sook Song,Eui-Sook Kim,Zhao-Hua Li,Yong-Hua Zhang,Jang-Mi Lee,Young-Joo Yi,Chang-Sik Park 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2
This study was carried out to investigate the effects of cryoprotectants, warming solution and removal of lipid on open pulled straw vitrification (OPS) method of porcine embryos produced by nuclear transfer (NT) of fetal fibroblasts. All solutions used during vitrification were prepared with holding medium consisting of 25 mM Hepes buffered TCM199 medium containing 20% fetal bovine serum (FBS) at 38.5℃. The blastocysts derived from NT with or without lipid were vitrified in each medium of different concentrations of dimethyl sulfoxide (DMSO) and ethylene glycol (EG). Also, blastocysts after cryopreservation were warmed into different concentrations of sucrose in warming solution. The optimal concentrations of cryoprotectants in vitrification solution were 10% DMSO + 10% EG in vitrification solution 1 (VS1) and 20% DMSO + 20% EG in vitrification solution 2 (VS2). The optimal concentrations of sucrose were 0.3 M sucrose in warming solution 1 (WS1) and 0.15 M sucrose in warming solution 2 (WS2). Lipid removal from oocytes before NT enhanced the viability of NT embryos after vitrification. Our results show that use of the OPS method in conjunction with lipid removal provides effective cryopreservation of porcine nuclear transfer embryos.
Triterpenes with cytotoxicity from the leaves of Vernicia fordii.
Pei, Yi-Hua,Kwon, Ok-Kyoung,Lee, Ji-Seon,Cha, Hyuk-Jin,Ahn, Kyung-Seop,Oh, Sei-Ryang,Lee, Hyeong-Kyu,Chin, Young-Won Pharmaceutical Society of Japan 2013 Chemical & pharmaceutical bulletin Vol.61 No.6
<P>Two new triterpenes, (1α,3β,8α,9β,10α,13α,14β)-9,10-dimethyl-25,26-dinorolean-5-en-1,3-diol (1) and (1α,3β,6β)-olean-12-en-1,3,6-triol (2) were isolated from the leaves of Aleurites fordii, together with five known triterpenes. The structures of isolates were established by one dimensional (1D)- and 2D-NMR spectroscopic data along with MS analysis. Of the isolated compounds, 1, 2 and 4 (daturadiol) displayed moderate cytotoxicities against two or more human cancer cell lines in HepG2 (hepatocellular carcinoma), SK-OV-3 (ovarian carcinoma), A-549 (lung carcinoma) and SNU-1 (gastric carcinoma).</P>
Computer simulation of stereochemical structure of biodegradable poly(d,l-lactide-co-l-lactide)
Pei-Hua He,Yan Wu,Ruo-Feng Wu 한국물리학회 2007 Current Applied Physics Vol.7 No.s1
poly(D,L-lactide-co-L-lactide) (PDLLA). The Monte Carlo model is set up according to the reactivity probabilities of two kinds of isomerRR and SS during ring-opening chain propagation. The simulation results for Tetrads and Hexads intensities are in good agreement withexperimental results for13C NMR. Some other important parameters for the stereochemical structure of PDLLA are also obtained fromRun numbers. The inuence of reactivity ratiorand feed ratiop on the stereochemical structure is analyzed and discussed in detail. Thiscomputer simulation method has proved to be a convenient method for establishing structureproperties relationships of certain poly-mers whose stereoregularity can be adjusted, such as PDLLA.
( Hua Li ),( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( Xue Ji Han ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-
Background: Opa interacting protein 5 (OIP5) encodes a 25- kDa protein with a coiled-coil domain. Previous studies have demonstrated the elevated expression of OIP5 mRNA in human gastric and colorectal carcinomas, but no report has functionally clarified the significance of OIP5 expression in human hepatocarcinogenesis and indicated its potential as a therapeutic target. The aim of study is to examine biological role of OIP5 in hepatocarcinogenesis. Methods: OIP5 mRNA expression was confirmed by Realtime- PCR analysis. OIP5 protein expression was investigated in HCC tissue and hepatoma cells by immunoblot and immunohistochemistry analysis. Cellular localization of OIP5 was detected by immunofluorescence. For the parallel determination of the relative levels of protein phosphorylation and mitogen- activated protein pinases (MAPKs) was experimented by the human phosphor-kinase array and (Phospho-MAPK) Array. Results: OIP5 abundantly expressed in tumor compared with non-tumor HCC tissues. OIP5 was localized in the nucleus and cytoplasm. Immunohistochemistry staining demonstrated that OIP5 was absent in normal liver, present in early and advanced tumours. Knockdown of OIP5 by shRNA specific to resulted in growth inhibition of hepatoma cell lines. Colony generation detected that OIP5 overexpression was approximate 2-fold more colony number. The knockdown of OIP5 resulted in decrease of soft agar colony generation. Phospho-AKT (s437) level was increased with OIP5 overexpression. OIP5 up- regulated in mitotic cell whereas OIP5 did not change in interphase. Conclusions: OIP5 may involved in hepatocarcinogenesis through Akt activation and tumor cell cycle progression.
( Pei-hua Chen ),( Rou-yun Chen ),( Jui-yu Chou ) 한국균학회 2018 Mycobiology Vol.46 No.1
Gray mold (Botrytis cinerea) is one of the most common diseases of strawberries (Fragaria×ananassa Duchesne) worldwide. Although many chemical fungicides are used for controlling the growth of B. cinerea, the risk of the fungus developing chemical resistance together with consumer demand for reducing the use of chemical fungicides have necessitated an alternative method to control this pathogen. Various naturally occurring microbes aggressively attack plant pathogens and benefit plants by suppressing diseases; these microbes are referred to as biocontrol agents. However, screening of potent biocontrol agents is essential for their further development and commercialization. In this study, 24 strains of yeast with antagonistic ability against gray mold were isolated, and the antifungal activity of the volatile and diffusible metabolites was evaluated. Putative mechanisms of action associated with the biocontrol capacity of yeast strains against B. cinerea were studied through in vitro and in vivo assays. The volatile organic compounds produced by the Galactomyces candidum JYC1146 could be useful in the biological control of plant pathogens and therefore are potential alternative fungicides with low environmental impact.
Zhang Pei-Pei,Liang Su-Xia,Wang Hua-Lun,Yang Kun,Nie Shao-Chen,Zhang Tong-Mei,Tian Yuan-Yuan,Xu Zhao-Yuan,Chen Wei,Yan Ying-Bin 한국통합생물학회 2021 Animal cells and systems Vol.25 No.5
The aim of this study was to compare the functional characteristics of mesenchymal stromal cells (MSCs) from a sheep model of traumatic temporomandibular joint (TMJ) fibrous and bony ankylosis. A sheep model of bilateral TMJ trauma-induced fibrous ankylosis on one side and bony ankylosis on the contralateral side was used. MSCs from fibrous ankylosed callus (FAMSCs) or bony ankylosed callus (BA-MSCs) at weeks 1, 2, 4, and 8 after surgery were isolated and cultured. MSCs derived from the bone marrow of the mandibular condyle (BM-MSCs) were used as controls. The MSCs from the different sources were characterized morphologically, phenotypically, and functionally. Adherence and trilineage differentiation potential were presented in the ovine MSCs. These cell populations highly positively expressed MSC-associated specific markers, namely CD29, CD44, and CD166, but lacked CD31 and CD45 expressions. The BA-MSCs had higher clonogenic and proliferative potentials than the FA-MSCs. The BA-MSCs also showed higher osteogenic and chondrogenic potentials, but lower adipogenic capacity than the FA-MSCs. In addition, the BA-MSCs demonstrated higher chondrogenic, but lower osteogenic capacity than the BM-MSCs. Our study suggests that inhibition of the osteogenic and chondrogenic differentiations of MSCs might be a promising strategy for preventing bony ankylosis in the future.