Survival and Performance of Two Cellulose-Degrading Microbial Systems Inoculated into Wheat Straw-Amended Soil

( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.

The Influence of New Service Means on Customer's Willingness to Buy under the Background of Artificial Intelligence Take the Marketing method of AI medical beauty APP as an example

Xiao-Pei Li(리시아오페이),Zi-Yang Liu(유자양) 한국컴퓨터정보학회 2020 韓國컴퓨터情報學會論文誌 Vol.25 No.9

본 논문의 목적은 “인공지능(AI)+의료미용 ”이라는 새로운 서비스 수단이 고객의 구매 의도에 미치는 영향하는 것이다. AI 의료뷰티 APP 마케팅 방식을 실증 연구로 한다. 본 논문은 SPSS 24.0 와 AMOS24.0 구조방정식 통계 소프트웨어를 이용하여 통계분석을 실시하였다. 분석방법은 신뢰성분석, 타당성분석, 구조방정식모형분석 등을 이용하였다. 실증연구를 통해 다음과 같은 결과를 얻었다. 1. AI의료용 뷰티 APP의 시스템품질은 유용성과 사용편의성에 긍정적인 영향을 미친다. 2. AI의료APP는 유용성과 사용편의성에 긍정적인 영향을 미친다. 3. AI의료용 뷰티 APP는 인식된 유용성과 인식된 사용편의성에 긍정적인 영향을 미친다. 4. 소비자가 인식된 사용 편의성은 인식된 유용성과 구매 의지에 긍정적인 영향을 미친다. 5. 소비자의 유용성을 알리는 것은 구매 의사에 긍정적인 영향을 미친다. The purpose of this paper is to study the influence of new service methods of "artificial intelligence (AI) + medical cosmetology" ,a new service means, on customers" purchase intentions. To AI medical beauty APP sales as an empirical study. This paper designed Likert seven scale to investigate, using SPSS 24.0 statistical analysis software and AMOS24.0 structural equation software to analyze the survey data. The analysis method uses reliability analysis, validity analysis, and construct equation model analysis. Through empirical research, the following results can be found ,1. The system quality of AI medical beauty app will have a positive impact on perceived usefulness and perceived ease of use. 2. The information quality of AI medical beauty app will have a positive impact on perceived ease of use and perceived usefulness. 3. The service quality of AI medical beauty app will have a positive impact on perceived ease of use and perceived usefulness 4. Consumers" perceived ease of use has a positive impact on perceived usefulness and purchase intention. 5. The usefulness of consumers" notification has a positive effect on purchase intention.

Aromatic Compounds from the Halotolerant Fungal Strain of Wallemia sebi PXP-89 in a Hypersaline Medium

Xiao-Ping Peng,Wei-Ming Zhu,Yi Wang,Pei-Pei Liu,Kui Hong,Hao Chen,Xia Yin 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.6

A new cyclopentanopyridine alkaloid, 3-hydroxy-5-methyl-5,6-dihydro-7H-cyclopenta[b]pyridin-7-one (1), together with 11 known aromatic compounds were isolated from the secondary metabolites of the halotolerant fungal strain Wallemia sebi PXP-89 in 10% NaCl. Their structures including the absolute configurations of (2S,3S)-1-(4-hydroxyphenyl)butane-2,3-diol (2), (2R,3S)-1-(4-hydroxyphenyl)butane-2,3-diol (3), and (S)-3-hydroxy-4-(4-hydroxyphenyl)-2-one (4) were elucidated by spectroscopic analysis and a modified Mosher’s method. Compound 1 exhibited antimicrobial activity against Enterobacter aerogenes with a MIC of 76.7 μM. The absolute configurations of compounds 2-4 were determined for the first time.

민속문화의 體現, 民間剪紙

裴小旗(Pei Xiao-qi) 중앙어문학회 2006 語文論集 Vol.35 No.-

Papercutting is an image carrier of Chinese folk culture. It is rich in history and has influenced people's life in every aspect. By means of certain art forms of papercutting such as calling-back-the-lost-soul and chichen-seizing-boy papercuttings, people express their wishes and desires for happiness and good fortune as well as the expelling of their bad luck and disasters. Besides, papercutting plays a very important part in the rituals of the folk life in China. For example, for the wedding people will cut various “happiness flowers” with bight red-paper to wish the new couple a happy life with healthy children. And at the funeral ceremony papercuttings serve as a guide for the dead to another world. Often a paradise, a reproduction of the real world, is created, as a benediction to the dead and a condolence to those whose beloved is dead. In addition, papercuttings have special significance at festivals and on holidays. At the New Year's Festival or the Lantern Festival, for example, they are used to heighten the joyous atmosphere and can reflect the psychology, aesthetic judgement and cultural orientation of the Chinese people. Papercutting, as a folk art, has become a indispensable part of people's life.

Monosomal Karyotypes among 1147 Chinese Patients with Acute Myeloid Leukemia: Prevalence, Features and Prognostic Impact

Yang, Xiao-Fei,Sun, Ai-Ning,Yin, Jia,Cai, Cheng-Sen,Tian, Xiao-Peng,Qian, Jun,Chen, Su-Ning,Wu, De-Pei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

A monosomal karyotype (MK), defined as ${\geq}2$ autosomal monosomies or a single monosomy in the presence of additional structural abnormalities, was recently identified as an independent prognostic factor conveying an extremely poor prognosis in patients with acute myeloid leukemia (AML). In the present study, after excluding patients with t(15;17), t(8;21), inv(16) and normal karyotypes, 324 AML patients with cytogenetic abnormalities were the main subject of analysis. The incidences of MK were 13% in patients aged 15 to 60 years and 18% in those between 15 and 88 years old. MK was much more prevalent among elderly patients (p < 0.001) and was significantly associated with the presence of -7, -5, del(5q), abn12p, abn17p, -18 or 18q-, -20 or 20q- and CK (for all p < 0.001 except for abn12p p=0.009), and +8 or +8q was less frequent in MK+ AML(p=0.007). No correlation was noted between monosomal karyotype and FAB subtype (p > 0.05); MK remained significantly associated with worse overall survival among patients with complex karyotype (p=0.032); A single autosomal monosomy contributed an additional negative effect in OS of patients with structural cytogenetic abnormalities (P=0.008). This report presents the prevalence, feature and prognostic impact of MK among a large series of Chinese AML patients from a single center for the first time.

Effects of Oxygen Content on the Simultaneous Microbial Removal of SO2 and NOx in Biotrickling Towers

Xing Chun Wang,Xiao Yi Bi,Pei Shi Sun,Jin Quan Chen,Ping Zou,Xiao Ming Ma,Jing Zhang,Hai Yu Wang,Xiao Yi Xu 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.5

This study uses microbial methods to research the influence of oxygen (O2) content on the removal efficiency of sulfur dioxide (SO2) and nitrogen oxides (NOx) in a tandem twin-towers desulfurization and denitrification process system. Oxygen can play a significant role in biotrickling towers. Other important factors had already been optimized prior to the study, including inlet concentration, gas flow rate, and temperature. SO2 and NOx were prepared by a chemical method. A gas flow meter was used to regulate nitrogen (N2) that had been stored in steel cylinders. In this way, the O2 content was adjusted in the biotrickling towers by controlling the N2 flow rate. Five gradients of O2 content were selected for investigation, namely 4, 8, 12, 16, and 20%. Results indicated that the SO2 removal efficiency from mixed gas (SO2 and NOx) can reach 100%, from all of the five O2 gradients, in biotrickling towers. In a tandem twin-towers desulfurization and denitrification process system, the NOx removal efficiency and the inlet concentration of nitrogen dioxide (NO2) gradually increased as the O2 content increased. Specifically, the average removal efficiency of NOx increased from 49.28 to 80.85% as the O2 content changed from 4 to 20%. The oxygen levels influenced the removal of NOx but the SO2 removal efficiency in mixed gas was always stable.

Characteristics of Morphological and Physiological Changes during the Autolysis Process of Saccharomyces cerevisiae FX-2

( Xiao Li ),( Xiaodan Shi ),( Man Zou ),( Yudi Luo ),( Yali Tan ),( Yexu Wu ),( Lin Chen ),( Pei Li ) 한국미생물생명공학회(구 한국산업미생물학회) 2015 한국미생물·생명공학회지 Vol.43 No.3

In this paper, the autolysis process of Saccharomyces cerevisiae FX-2 (S. cerevisiae FX-2) via, a variety of endogenous enzyme, was investigated systematically by analyzing changes in physicochemical parameters in autolysate, surface morphology and the internal structure of the yeast cells. As an explicit conclusion, the arisen autolysis depended on the pH and the optimal pH was found to be 5.5. Based on the experimental data and the characteristics of mycelia morphology, a hypothesis is put forward that simple proteins in yeast vacuolar are firstly degraded for utilization, and then more membrane- bound proteins are hydrolyzed to release hydrolytic enzymes, which arouse an enzymatic reaction to induce the collapse of the cell wall into the cytoplasm.

Asymmetric Synthesis of the Sex Pheromones of Pine Sawflies and Other Semichemicals

Pei-Qiang Huang,Hong-Qiao Lan,Xiao Zheng,Yuan-Ping Ruan 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

Distributed Dual-rate Consensus Predictive Control of Looper Tension System in Hot Rolling Mills

Xiao-Dong Zhang,Shao-Shu Gao,Xin-Ping Liu,Ting-Pei Huang 제어·로봇·시스템학회 2018 International Journal of Control, Automation, and Vol.16 No.2

This paper considers a dual-rate distributed predictive control strategy for the looper tension system in hot rolling mills, which is a typical multi-agent system with directed communication topology. First, we establish an interconnected model for looper tension control system and the disturbances from the neighbors are considered effectively. Second, the consensus control protocol is developed based on the proposed control strategy to improve the robustness and stability of the multi-agent, and the sufficient conditions for consensus are developed. We update and implement all the agent controllers sequentially in one output sampling period and begin a new cycle at the next sampling instant, which leads the multi-agent control system is of fast control updating rate and slow output sampling rate. The control inputs of neighbors can be obtained to compensate the coupling effects, and the cooperation of controllers are improved. Finally, simulation results verify the proposed control strategy and corresponding results.