The mitogen-activated protein kinase phosphatase-1 (MKP-1) gene is a potential methylation biomarker for malignancy of breast cancer

Fang-Ming Chen,Hsueh-Wei Chang,Sheau-Fang Yang,Ya-Fang Huang,Pei-Yung Nien,Yao-Tsung Yeh,Ming-Feng Hou 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.5

The mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) belongs to the MAPK cascades which are central to cell proliferation and apoptosis. The carcinogenic role of MKP-1 has been reported in many types of cancer but it has rarely been investigated in breast cancer. The present study was designed to evaluate the MKP-1 mRNA expression and its possible regulation by methylation of MKP-1 promoter in the model of several breast cancer cell lines and tissues as well as controls. Our data demonstrate MKP-1mRNA expression significantly decreased in five breast cancer cell lines compared to breast controls (P <0.01). Using the methylation-specific PCR (MSP) analysis,the unmethylated reaction (U) is dominant in both normal cell lines and benign breast tumors (100% vs. 86.2%), whereas the methylated reaction (M) is dominant in both breast cancer cell lines and invasive breast tumors (100% vs. 57.2%). In terms of methylation ratio (M/M+U), methylation level in MKP-1 promoter is significantly higher in the invasive breast tumor tissues (n = 152) than in benign breast tumor tissues (n = 29)(P < 0.0001). Assessing the methylation ratio of the promoter of MKP-1 gene to diagnose the breast malignancy (invasive vs. benign), the area under the receiver-operating characteristic (ROC) curve was 0.809(95% CI: 0.711-0.906, P < 0.001). The best performance for this prediction has a sensitivity of 76.32% and a specificity of 82.76% at the cutoff value of 0.38. Taken together, we firstly demonstrated that the promoter methylation of MKP-1 gene is a potential breast cancer biomarker for breast malignancy.

브라운필드의 지속가능한 재개발에 관한 사례 연구

배방명(Pei, Fang Ming),김신원(Kim, Shin Won) 한국디자인문화학회 2018 한국디자인문화학회지 Vol.24 No.1

최근 새로운 도시 경관의 조성에 있어서 브라운필드 재개발의 중요성이 증대되고 있다. 이에 본 연구는 브라운필드 재개발의 실태를 파악함으로써 도시 환경의 질적인 향상을 위한 브라운필드의 가치를 재조명하고, 지속가능한 브라운필드 재개발을 위한 방법을 제시하는 데에 목적을 둔다. 연구의 범위는 기존의 우수한 브라운필드 재개발의 대표적인 사례를 선정한다. 연구의 방법으로 브라운필드의 정의, 지속가능한 발전의 개념에 대해 이론적으로 고찰하였다. 브라운필드 재개발에 대해 환경적 및 사회적 인식문제를 토의하였다. 문헌연구를 통해 브라운필드의 지속가능한 발전방식과 지역사회 환경의 연계성을 향상시키고 나아가 지역발전을 기대할 수 있다는 것을 알 수 있다. 대상지를 재개발 시기에 따라 순차적으로 살펴보았으며 연구사례는 도시의 구조적, 시대적, 사회적 요건등을 기준으로 선정하였다. 선정된 대상지의 공통점과 개선점을 비교, 각 사례의 지속가능 여부를 평가하고, 향후 브라운필드 재개발의 방향성 및 브라운필드 재개발에 영향을 끼치는 주요 요인에 관해 모색하고자 하였다. 연구 결과, 활성화 교류장소를 제공하는 재개발 지역은 고도의 지속가능적 발전성을 유지할 수 있는 결과를 낳았다. 연구에서 얻은 브라운필드 재개발과 다양한 커뮤니티와의 조화라는 개념 즉, 사람과 자연환경 간의 상호 작용은 브라운필드의 지속가능한 재개발에 제시된 필요성이 있다고 판단된다. 도시공간에 긍정적인 영향을 미치는 브라운필드의 재개발 인식전환에 따라 문화적, 경제적, 역사적 재개발이 지속가능하기를 기대하며 본 논문이 향후 관련 연구에 가치있는 자료로 활용되기를 기대한다. The importance of the Brownfield redevelopment in recent years has been increasing to establish a new formation of urban landscapes. Therefore the study aims to put forward a proposal about manufacture values of brownfield through identifying the status of brownfield retrofits to improve the quality of the urban environment and to propose methods to enhance the sustainability of Brownfield redevelopment. To achieve this, three representative examples of the existing excellent brownfield redevelopment are selected in the research scope. The study was mainly performed by analyzing survey data related to the definition of Brownfield, the concept of sustainable development and it conducted a discussion of environmental and social awareness issues on brownfield redevelopment. Through the literature review, it can be seen that it is significant to improve the linkage between the sustainable development of Brownfield and the community environment to revitalizing development of the regional community. The case study was carried out based on time, and the study case was based on the requirements of city structure, period, and social requirements. The purpose of this study is to evaluate the sustainability of Brownfield redevelopment in each case by comparing common points and improvements for the research sites and to investigate the main factors influencing redevelopment direction of the Brownfield redevelopment. The results show that redevelopment areas with full of dynamic characteristic were able to maintain the high capability of the sustainable development. In conclusion, it is critical to understand the harmonization between brownfield redevelopment and various communities such as the interaction between people and the natural environment in order to provide the direction for the design approaches of the sustainable redevelopment of Brownfield. Cultural, economic, and historical redevelopment is central to revitalizing the community and have a positive impact on urban space. The researcher wish that this study will be used as the theoretical analysis data in developing sustainable redevelopment of the Brownfield.

Monitoring of genetically close Tsaiya duck populations using novel microsatellite markers with high polymorphism

Lai, Fang-Yu,Chang, Yi-Ying,Chen, Yi-Chen,Lin, En-Chung,Liu, Hsiu-Chou,Huang, Jeng-Fang,Ding, Shih-Torng,Wang, Pei-Hwa Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.6

Objective: A set of microsatellite markers with high polymorphism from Tsaiya duck were used for the genetic monitoring and genetic structure analysis of Brown and White Tsaiya duck populations in Taiwan. Methods: The synthetic short tandem repeated probes were used to isolate new microsatellite markers from the genomic DNA of Tsaiya ducks. Eight populations, a total of 566 samples, sourced from Ilan Branch, Livestock Research Institute were genotyped through novel and known markers. The population genetic variables were calculated using optional programs in order to describe and monitor the genetic variability and the genetic structures of these Tsaiya duck populations. Results: In total 24 primer pairs, including 17 novel microsatellite loci from this study and seven previously known loci, were constructed for the detection of genetic variations in duck populations. The average values for the allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.29, 5.370, 0.591, 0.746, and 0.708, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting Brown Tsaiya duck cluster and a spreading White Tsaiya duck cluster. The Brown Tsaiya ducks and the White Tsaiya ducks with Pekin ducks were just split to six clusters and three clusters when K was set equal to 6 and 3 in the Bayesian cluster analysis. The individual phylogenetic tree revealed eight taxa, and each individual was assigned to its own population. Conclusion: According to our study, the 24 novel microsatellite markers exhibited a high capacity to analyze relationships of inter- and intra-population in those populations with a relatively limited degree of genetic diversity. We suggest that duck farms in Taiwan could use the new (novel) microsatellite set to monitor the genetic characteristics and structures of their Tsaiya duck populations at various intervals in order to ensure quality breeding and conservation strategies.

The Effect of Agomelatine in Behavioral and Psychological Symptoms of Dementia

Carol Sheei-Meei Wang(Carol Sheei-Meei Wang ),Kuo-Sheng Cheng(Kuo-Sheng Cheng),Chia-Hung Tang(Chia-Hung Tang),Ming-Chyi Pai(Ming-Chyi Pai),Pai-Lien Chen(Pai-Lien Chen),Pei-Fang Chien(Pei-Fang Chien) 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.4

Objective: Moderate and severe behavioral and psychological symptoms of dementia (BPSD) often need medical treatment to improve symptoms. Agomelatine is a selective melatonergic (MT1/MT2) agonist that has normalizing effects on disturbed circadian rhythms and disrupted sleep−wake cycles. Its activity of 5HT-2C receptor antagonism is associated with lessening depression and anxiety and increasing slow-wave sleep. Based on past clinical records and current findings it suggests that agomelatine can improve BPSD for patients. This retrospective cohort study was designed to compare the BPSD before and after using agomelatine. Methods: Records of dementia cases who had ever received agomelatine treatment for BPSD in a general hospital setting during the past 2.5 years were identified and reviewed. Scores from before and after 3 months of treatment with agomelatine were collected for Neuropsychiatric Inventory (NPI), Brief Psychiatric Rating Scale (BPRS), and Clinical Global Impression (CGI) to compare and analyze the difference of psychological and behavioral symptoms pre- and post-agomelatine used. Results: Records of 144 cases of dementia with BPSD who had ever used agomelatine from January 2015 to June 2017 were collected. All of the 112 cases had BPRS and CGI scores, of which 75 cases had additional NPI scores. Among these 112 cases, the BPRS and CGI scores were significantly improved in all types of dementia. NPI scores indicated that the use of agomelatine alleviated obvious symptoms and decreased overall distress, especially in the depression/poor mood, anxiety, and sleep/night behavior. Conclusion: It is consistent with an effective result of agomelatine in improving BPSD.

Survey of genetic structure of geese using novel microsatellite markers

Fang-Yu Lai,Po-An Tu,Shih-Torng Ding,Min-Jung Lin,Shen-Chang Chang,En-Chung Lin,Ling-Ling Lo,Pei-Hwa Wang 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: The aim of this study was to create a set of microsatellite markers with high polymorphism for the genetic monitoring and genetic structure analysis of local goose populations. Methods: Novel microsatellite markers were isolated from the genomic DNA of white Roman geese using short tandem repeated probes. The DNA segments, including short tandem repeats, were tested for their variability among four populations of geese from the Changhua Animal Propagation Station (CAPS). The selected microsatellite markers could then be used to monitor genetic variability and study the genetic structures of geese from local geese farms. Results: 14 novel microsatellite loci were isolated. In addition to seven known loci, two multiplex sets were constructed for the detection of genetic variations in geese populations. The average of allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.09, 5.145, 0.499, 0.745, and 0.705, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting white Roman cluster and a spreading Chinese cluster. In white Roman populations, the CAPS populations were depleted to roughly two clusters when K was set equal to 6 in the Bayesian cluster analysis. The founders of private farm populations had a similar genetic structure. Among the Chinese geese populations, the CAPS populations and private populations represented different clads of the phylogenetic tree and individuals from the private populations had uneven genetic characteristics according to various analyses. Conclusion: Based on this study’s analyses, we suggest that the CAPS should institute a proper breeding strategy for white Roman geese to avoid further clustering. In addition, for preservation and stable quality, the Chinese geese in the CAPS and the aforementioned proper breeding scheme should be introduced to geese breeders.

Evaluation of expression of the Wnt signaling components in canine mammary tumors via RT2 Profiler PCR Array and immunochemistry assays

Fang Yu,Roberta Rasotto,Hong Zhang,Shimin Pei,Bin Zhou,Xu Yang,Yipeng Jin,Di Zhang,Degui Lin 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.3

The Wnt signaling pathway and its key component b-catenin have critical roles in the development of diseases such as tumors in mammals. However, little has been reported about involvement of the Wnt/b-catenin signaling pathway in canine mammary tumors (CMTs). The present study detected expression of 30 Wnt signaling pathway-related genes in CMTs; the results are potentially useful for molecular-based diagnosis of CMTs and the development of new targeted therapies. Significant upregulations of dickkopf-1 protein, secreted frizzled-related sequence protein 1 (SFRP1), frizzled 3, b-catenin, and lymphoid enhancer-binding factor 1 (LEF1) were detected in highly malignant CMTs compared to levels in normal mammary gland tissues; moreover, highly significant upregulation of WNT5A was observed in low malignancy CMTs. Downregulation was only detected for SFRP4 in malignant CMT samples. The subcellular location of b-catenin and cyclin D1 in 100 CMT samples was investigated via immunohistochemical analysis, and significantly increased expressions of b-catenin in cytoplasm and cyclin D1 in nuclei were revealed. Western blotting analysis revealed that the expression of b-catenin and LEF1 increased in in the majority of CMT samples. Taken together, the results provide important evidence of the activation status of the Wnt pathway in CMTs and valuable clues to identifying biomarkers for molecular-based diagnosis of CMT.

Colorectal Cancer Mortality Characteristics and Predictions in China, 1991-2011

Fang, Jia-Ying,Dong, Hong-Li,Sang, Xue-Jin,Xie, Bin,Wu, Ku-Sheng,Du, Pei-Ling,Xu, Zhen-Xi,Jia, Xiao-Yue,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: To identify the epidemiological characteristics of colorectal cancer mortality in China during the period of 1991-2011, and forecast the future five-year trend. Materials and Methods: Mortality data for colorectal cancer in China from 1991 to 2011 was used to describe epidemiological characteristics in terms of age group, gender, and rural/urban residence. Trend surface analysis was performed to analyze the geographical distribution of colorectal cancer. Four models including curve estimation, time series modeling, gray modeling and joinpoint regression were applied to forecast the trends for the future five years. Results: Since 1991 the colorectal cancer mortality rate increased yearly, and our results showed that the trend would continue to increase in the ensuing 5 years. The mortality rate in males was higher than that of females and the rate in urban areas was higher than in rural areas. The mortality rate was relatively low for individuals less than 60 years of age, but increased dramatically afterwards. People living in the northeastern China provinces or in eastern China had a higher mortality rate for colorectal cancer than those living in middle or western China provinces. Conclusions: The steadily increasing mortality of colorectal cancer in China will become a substantial public health burden in the foreseeable future. For this increasing trend to be controlled, further efforts should concentrate on educating the general public to increase prevention and early detection by screening. More effective prevention and management strategies are needed in higher mortality areas (Eastern parts of China) and high-risk populations (60+ years old).

Characteristics and Prediction of Lung Cancer Mortality in China from 1991 to 2013

Fang, Jia-Ying,Dong, Hong-Li,Wu, Ku-Sheng,Du, Pei-Ling,Xu, Zhen-Xi,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Objective: To describe and analyze the epidemiological characteristics of lung cancer mortality in China from 1991 to 2013, forecast the future five-year trend and provide scientific evidence for prevention and management of lung cancer. Materials and Methods: Mortality data for lung cancer in China from 1991 to 2013 were used to describe epidemiological characteristics. Trend surface analysis was applied to analyze the geographical distribution of lung cancer. Four models, curve estimation, time series modeling, gray modeling (GM) and joinpoint regression, were performed to forecast the trend for the future. Results: Since 1991 the mortality rate of lung cancer increased yearly. The rate for males was higher than that for females and rates in urban areas were higher than in rural areas. In addition, our results showed that the trend will continue to increase in the ensuing 5 years. The mortality rate increased from age 45-50 and peaked in the group of 85 years old. Geographical analysis indicated that people living in northeast China provinces and the coastal provinces in eastern China had a higher mortality rate for lung cancer than those living in the centre or western Chinese provinces. Conclusions: The standardized mortality rate of lung cancer has constantly increased from 1991 to 2013, and been predicted to continue in the ensuing 5 years. Further efforts should be concentrated on education of the general public to increase prevention and early detection. Much better prevention and management is needed in high mortality areas (northeastern and eastern parts of China) and high risk populations (45-50-year-olds).

The Case Studies of Small Intestine Transplantation

Pei-Ling Hsieh,Yu-Wen Fang,Mei-Hua Wang 한국간호과학회 2011 한국간호과학회 학술대회 Vol.2011 No.10

Liver Cancer Mortality Characteristics and Trends in China from 1991 to 2012

Fang, Jia-Ying,Wu, Ku-Sheng,Zeng, Yang,Tang, Wen-Rui,Du, Pei-Ling,Xu, Zhen-Xi,Xu, Xiao-Ling,Luo, Jia-Yi,Lin, Kun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Purpose: To investigate the distribution of liver cancer mortality as well as its developing trend from 1991 to 2012, forecast the future five-year trend, and provide a basis for the comprehensive prevention and management. Materials and Methods: Mortality data for liver cancer in China from 1991 to 2012 were used to describe characteristics and distribution of liver cancer mortality. Trend surface analysis was used to study the geographical distribution of liver cancer mortality. Curve estimation, time series modeling, gray modeling (GM) and joinpoint regression were used to predict and forecast future trends. Results: The mortality rate of liver cancer has constantly increased in China since 1991. Rates in rural areas are higher than in urban areas, and in males are higher than in females. In addition, our data predicted that the trend will continue to increase in the next 5 years. The age-specific mortality of liver cancer increases with age and peaks in the group of 80-84 years old. Geographical analysis showed the liver mortality rate was higher in the southeast provinces, such as Jiangsu, Zhejiang and Guangdong, and southwest regions like Guangxi Province. Conclusions: The standardized mortality rate of liver cancer in China has consistently increased from 1991 to 2012, and the upward trend is predicted to continue in the future. Much better prevention and management of liver cancer is needed in high mortality areas (the southwestern and southeastern parts of China) and high mortality age groups (80- to 84-year-olds), especially in rural areas.