關于對中國文學史的宏觀與微關硏究

章培恒 中國語文硏究會 1998 中國語文論叢 Vol.15 No.-

림프질 전이가 있는 하인두암과 하부식도암의 진단에 활용된 내시경초음파 유도하 Trucut 생검 1예

광명옥,이정환,문정섭,김유선,류수형,총배천,김형훈,김현태 仁濟大學校 白病院 2010 仁濟醫學 Vol.31 No.-

The incidence of double primary cancer is about 10% in all kinds of cancers. Double primary cancer is usually accompanied by gastrointestinal cancer. Esophageal cancer is supposed to be frequently accompanied by squamous cell carcinoma of the head and neck or the upper respiratory tract, because of such common carcinogens as smoking and alcohol ingestion. Esophageal cancer and hypopharyngeal cancer are usually diagnosed by esophagogastric endoscopy with biopsy. We performed endoscopic ultrasound-guided Trucut biopsy(EUS-TCB) to diagnose a mediastinal mass in a 49 year-old patient, who visited our hospital because of voice change. During procedure, we accidently found enlarged celiac lymph node, and obtained tissue from the mediastinal lymph node and the celiac lymph node. We confirmed double primary cancer of squamous cell carcinoma in the esophagus and the hypopharynx via esophagogastric endoscopy.

Basic, HCC basic : PE-108 ; Progenitor cell-derived hepatocytes and their characteristics in human

( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( In Hee Kim ),( Dae Ghon Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background: Hepatic progenitor cells (HPCs) are capable of differentiating along the hepatic lineage into hepatocytes or cholangiocytes (bile duct cells), hence play a critical role in the process of liver regeneration. Their biological discrimination and characterization are critical for therapeutic potential. Aims of this study is to establish progenitor cell-derived hepatocytes and to characterize their specific markers. Methods: Potential liver progenitor cells (HNK-1) were established and their various HPC protein expressions were investigated by immunoblotting, immunofluorescence and fluorescence-activated cell sorting (FACS) analyses, compared with those of other HCC cells. Immunohistochemistry was performed to detect these HPC antigen expression in the tissues of hepatic cirrhosis. Anchorage-independent growth and tumorigenicity were determined using soft agar and xenograft assay. Results: The HNK-1 cells highly expressed HPC markers such as EpCAM, CK7, CK19, AFP, CK8, CK18, EFNA1, and Thy1. Whereas, CD133 was barely expressed. In contrast, malignant Hep3B cells were positive in both EpCAM and CD133. Ductular reactions at the periphery of the cirrhotic nodules were immunohistochemically positive for these HPC markers. Sodium butyrate could induce hepatocyte-like morphological changes in HNK-1 cells, accompanying down-regulation of the hepatic progenitor cell markers (EpCAM, CK7, CK19, and EFNA1) and up-regulation of mature hepatocyte markers (albumin, CK8, and CK18) in both dose-dependent and time- dependent manners. Colony formation in vitro and tumorigenesis in vivo showed that there were no tumorigenesis capacity in EpCAM (+)/CD133(-) HNK1cells at the 0-2nd, 10th, 25th, and 50th passages, while the positive control EpCAM (+)/CD133(+) Hep3B cells could induce tumor in the mice model. Conclusions: Taken together, our results suggest that HNK1 cells are progenitor cell-derived hepatocytes and their stemmness- related markers EpCAM (+)/CD133(-) may be a distinguished marker for nonmalignant, progenitor cell-derived hepatocytes.

中國舞蹈敎育現狀分析

Pei Pei Tian 한국무용연구학회 2010 한국무용연구 Vol.28 No.1

Thanks to popularization of dance education and its spread over institutions such as art centers, culture centers, palaces for the youth(the organizations for political and cultural activities), universities, and middle schools, people of all social classes in China have formed a complete education system for dance and strengthened it for a few years. This study analyzes the current condition of dance education in China, based on Beijing dance academy and Beijing university of teaching, suggests the features of dance education in both schools; it investigates dance, classification and curriculum in the middle/high schools attached to Beijing dance academy and explores the dance education in primary schools and outside schools. Beijing dance academy had been founded in 1954 and was ratified in 1978 by Ministry of Culture. Currently, it has five majors (acting, choreography, dance studies, visual art design of acting, and management of public utilities), 9 dance departments, a center for life-long education, a center for training teachers, dance troupe of the youth, and a secondary dance school. The academy has an authority to grant a master degree of dance and qualification for training students with a master degree of research in 36 fields. Each department has different purpose and training course; there is a distinction between Chinese traditional dance and dance of Chinese people in educational objective. Beijing university of teaching was established in 1954; dance department belongs to Music School in the university; the school is designed to develop talent, competent students, by means of continuous dance performance, courses for creating dance movement, basic course, and courses for dance theories. The major classes consist of ballet, Chinese dance, dance of Chinese people, modern dance, dance choreography, production, creation, repertoire, dance theories, and sports dance; the courses of dance theories are composed of analysis of works, the history of dance in China and foreign countries, dance esthetics, a methodology of dance for Chinese people, an introduction to art, and the department of business administration for dance art. Dance in China is largely divided higher into vocational education, aiming to develop capable dancers; they are encouraged to realize their full potentials, based on acquisition of a vast basics and in-depth knowledge. Looking into the subject classification and curricular assignment in the middle, high schools attached to Beijing dance academy, dance education in China is mostly focused on training both amateurs in universities and professionals of the organizations concerned; there are about 108 secondary dance schools in a city. China is changing rapidly in the 21st century; it is believed that science has a important role in China's prosperity, with education being fundamental; organization, integration of dance education has come as a new challenge. Faced with the era of innovation, development, the dance business in China is said to be the age of science. For improvement in China dance, more systematic approach to it is required.

Dynamics of Idiosyncratic Volatility and Market Volatility: An Emerging Market Perspective

Pei Pei Tan,Don U.A. Galagedera 연세대학교 동서문제연구원 2015 Global economic review Vol.44 No.1

Estimating idiosyncratic volatility (IVOL) using various model-dependent and model-independent measures, we investigate the characteristics of aggregate IVOL in Malaysia over the period 1990–2008. The IVOL estimated in all models have similar patterns and has no trend over the sample period. There is evidence of episodic phenomenon. During financial crisis periods, market volatility is relatively higher than IVOL – a plausible reason is high correlation between firms’ returns. Small firms and low-priced stocks appear to influence IVOL more than large firms and high-priced stocks. In Malaysia, market volatility and IVOL may predict GDP growth.

PEI Induced Aggregation of Silver nanoparticles (AgNPs) Enhanced Fluorescence of Europium-Tetracycline (EuTc) complex for Selective and Sensitive Detection of Cu<SUP>2+</SUP> in Water

Pei LI,Hyun Gyu PARK 한국생물공학회 2018 한국생물공학회 학술대회 Vol.2018 No.10

Survival and Performance of Two Cellulose-Degrading Microbial Systems Inoculated into Wheat Straw-Amended Soil

( Pei Pei Li ),( Dong Dong Zhang ),( Xiao Juan Wang ),( Xiao Fen Wang ),( Zong Jun Cui1 ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1

A cellulose-degrading composite microbial system containing a mixture of microbes was previously shown to demonstrate a high straw-degrading capacity. To estimate its potential utilization as an inoculant to accelerate straw biodegradation after returning straw to the field, two cellulose-degrading composite microbial systems named ADS3 and WSD5 were inoculated into wheat straw-amended soil in the laboratory. The microbial survival of the inoculant was confirmed by a denaturing gradient gel electrophoresis (DGGE) analysis, whereas the enhancement of straw degradation in soil was assessed by measuring the mineralization of the soil organic matter and the soil cellulase activity. The results indicated that most of the DGGE bands from ADS3 were detected after inoculation into straw-amended autoclaved soil, yet only certain bands from ADS3 and WSD5 were detected after inoculation into straw-amended non-autoclaved soil during five weeks of incubation; some bands were detected during the first two weeks after inoculation, and then disappeared in later stages. Organic matter mineralization was significantly higher in the soil inoculants ADS3 and WSD5 than in the uninoculated controls during the first week, yet the enhanced degradation did not persist during the subsequent incubation. Similar to the increase in soil organic matter, the cellulase activity also increased during the first week in the ADS3 and WSD5 treatments, yet decreased during the remainder of the incubation period. Thus, it was concluded that, although the survival and performance of the two inoculants did not persist in the soil, a significant enhancement of degradation was present during the early stage of incubation.

Basic, Research : Isolation of EpCAM+/CD133? Hepatic Progenitor Cells

( Pei Pei Hao ),( Mi Jin Lee ),( Goung Ran Yu ),( In Hee Kim ),( Dae Ghon Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background/Aim: Hepatic progenitor cells (HPCs) are capable of differentiating along the hepatic lineage into hepatocytes or cholangiocytes. Progenitor cell-derived hepatocytes are critical for hepatocyte replenishment. Therefore, we have established human hepatic progenitor cells (HNK1) and determined their biological characteristics for experimental and therapeutic applications. Methods: Potential liver progenitor cells (HNK1) were established and their various HPC protein expressions were investigated by immunoblotting, immunofluorescence and fluorescence- activated cell sorting (FACS) analyses, compared with those of other HCC cells. Immunohistochemistry was performed to detect these HPC antigen expression in the tissues of hepatic cirrhosis. Albumin, ureagenesis and CYP450 activity were measured. Anchorage-independent growth and tumorigenicity were determined using soft agar and xenograft assay. Genetic constitution of the HNK1 was examined by karyotyping. Chromaosomal rearrangements at metaphase were detedted by Giemsa banding. Results: The HNK1 cells highly expressed HPC markers such as EpCAM, CK7, CK19, AFP, CK8, CK18, EFNA1, and Thy1. Whereas, CD133 was barely expressed. In contrast, malignant Hep3B cells were positive in both EpCAM and CD133. Ductular reactions at the periphery of the cirrhotic nodules were immunohistochemically positive for these HPC markers. Sodium butyrate could induce hepatocyte-like morphological changes in HNK1 cells, accompanying down-regulation of the hepatic progenitor cell markers (EpCAM, CK7, CK19, and EFNA1) and up-regulation of mature hepatocyte markers (albumin, CK8, and CK18). Albumin, ureagenesis, and CYP450 activity were also significantly increased by serial passages after treatment with sodium butyrate. Colony formation in vitro and tumorigenesis in vivo showed that there were no tumorigenesis capacity in EpCAM (+)/CD133(-) HNK1 cells at the 0-2nd,10th,25th,and 50th passages, while the positive co ntrol EpCAM (+)/CD133(+) Hep3B cells could induce tumor in the mice model. Conclusions: HNK1 cells were found to be EpCAM+/CD133? hepatic progenitor cells without spontaneous malignant transformation ability that could be useful for experimental and therapeutic applications. Moreover, EFNA1 should be recognized as an HPC marker.

Hepatic differentiation of the human hepatic progenitor cells (HPCs) by sodium butyrate (초)

( Pei Pei Hao ),( Xiang Dan Cui ),( Mi Jin Lee ),( Gyung Ran Yu ),( Dae Ghon Kim ) 대한간학회 2010 Clinical and Molecular Hepatology(대한간학회지) Vol.16 No.3(S)

Improvement of Biomineralization of Sporosarcina pasteurii as Biocementing Material for Concrete Repair by Atmospheric and Room Temperature Plasma Mutagenesis and Response Surface Methodology

( Pei-pei Han ),( Wen-ji Geng ),( Meng-nan Li ),( Shi-ru Jia ),( Ji-long Yin ),( Run-ze Xue ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.9

Microbially induced calcium carbonate precipitation (MICP) has recently become an intelligent and environmentally friendly method for repairing cracks in concrete. To improve on this ability of microbial materials concrete repair, we applied random mutagenesis and optimization of mineralization conditions to improve the quantity and crystal form of microbially precipitated calcium carbonate. Sporosarcina pasteurii ATCC 11859 was used as the starting strain to obtain the mutant with high urease activity by atmospheric and room temperature plasma (ARTP) mutagenesis. Next, we investigated the optimal biomineralization conditions and precipitation crystal form using Plackett-Burman experimental design and response surface methodology (RSM). Biomineralization with 0.73 mol/l calcium chloride, 45 g/l urea, reaction temperature of 45°C, and reaction time of 22 h, significantly increased the amount of precipitated calcium carbonate, which was deposited in the form of calcite crystals. Finally, the repair of concrete using the optimized biomineralization process was evaluated. A comparison of water absorption and adhesion of concrete specimens before and after repairs showed that concrete cracks and surface defects could be efficiently repaired. This study provides a new method to engineer biocementing material for concrete repair.